LASER SCANNING CONFOCAL MICROSCOPES (LSCM)
Zeiss 880
Zeiss 880 LSCM
Orientation: Inverted, objectives are positioned below the stage where your sample is placed.
Location: This microscope rig is located in the new Cellular Analysis Facility, Rm L113 - basement of the Cardiovascular Building.
Booking access: Approved users (after induction/training) can book this instrument (SMB LSM880) online by accessing the facility's online booking system (BookMyLab) at https://www.cvr-sgdb-bookings.org/.
User training:
The suite have dedicated staff available for support-:
Leandro.LemgruberSoares@glasgow.ac.uk; Susan.Baillie@glasgow.ac.uk; Anthony.Dornan@ glasgow.ac.uk.
Cost details: Email Anthony Dornan direct for details at Anthony.Dornan@glasgow.ac.uk
Laser lines: 405, 458, 488, 514, 561, 594 & 633 nm.
Main beam splitters: 405, 445, 458, 458/514, 458/561, 458/514/594, 488, 488/561, 488/594, 488/561/633.
Secondary: T80/R20, Plate.
Tuneable spectral fluorescence detectors: Zeiss 880 has been fitted with a 3 channel QUASOR emission tuneable spectral detector unit for filter free spectral emission detection over the wavelength range from 400-800 nm. The channel 1 side located spectral detector is a green sensitive PMT and the opposing side channel detector is red sensitive PMT. A central tuneable GaAsP detector, (channel 2), is also available for detecting weak fluorescence signals emitted from dim fluorescence samples. The red sensitive side PMT and GaAsP detector can also record fluorescence as a read out of single photons as well as analog mode. A transmitted light PMT is also available for performing bright field/DIC imaging.
Fluorophores: system is ideal for exciting dyes whose excitation peak match the laser lines available e.g. DAPI, HOECHST, mECFP, mCerulean, mTeal, mEGFP, mEYFP, mVenus, Alexa Fluor 488/546 /633/635/647. Rhodamine, Texas Red,).
Eye piece turret viewing epi-fluorescence filter sets-: DAPI/HOECHST Zeiss Filter set 49; EGFP/FITC-Green Zeiss filter set 38; DsRed Red Zeiss Filter set 43.
Microscope: Zeiss Axio Observer Z1, (invert configuration) with adjustable intensity metal halide lamp for wide field eyepiece fluorescence illumination viewing. Stage holder sample carrier available for fitting slides, Labtek coverglass chambers and petri dishes (maximum diameter 60 mm).
Objective lens: 10x Plan-Aprochromat, NA = 0.45, working distance = 2.0mm, 20x Plan- Apochromat. NA = 0.80, air, working distance = 0.55mm, coverglass; 40x Plan-Apochromat, NA = 1.30, oil, DIC, working distance = 0.21mm; 63x Plan-Apochromat, NA: 1.40, oil, DIC, working distance = 0.14 mm. Optional 5x Epiplan Neoflour air, NA = 0.13, working distance = 14.5 mm.
Capabilities: Ideal for automated high resolution timelapse z-stack multifluorescence imaging. Motorised stage can be used for multi position tile scanning. Scan speeds up to 13 fps possible for 512 x 512 acquisition. Lasers can be used for ROI defined photoactication of PA-dyes or to bleach regions of interest to perform Acceptor photobleaching FRET or FRAP experiments. Laser beam scanning modes available are line, frame, fast frame and spot. Lambda spectral scan image collection can also be set up. Fully motorised XY stage for multi-position imaging, tiling and mosaic acquisition.
Computer: HP Z840 workstation fitted with dual 3GHz Xeon processors, 128 Gb RAM and 2 Gb graphics card. Operating system is Microsoft Windows 10 Professional. Zeiss acquisition software is Zen Black Software, release version 2.1. Zen Blue software (2.1) is also available for offline image analysis.
Please find the user guide for this system here: Final Zeiss 880 start up
Leica TCS SP8
Leica TCS SP8 LSCM
Orientation: Inverted, objective lenses are positioned below the stage where your sample is placed.
Location: This instrument is located in the Bower Building. Access to the instrument via the online booking system will be granted once you have completed your training session to gain approved user status.
Training: To arrange user training email Prof. Micharl Blatt direct: (michael.blatt@glasgow.ac.uk). Training is available for PhD students and staff only. We will not support undergraduate use of the microscope except when accompanied by a registered user (in which case, it is the registered user who will operate the system).
Booking access: For approved users, book online, during normal working hours (9-5, Mon-Fri).
Cost & contact details: £35 per hour. Prior credit purchase required before use. Credit charges on a discounted sliding scale. Email Professor Michael Blatt for discount details.
Continous wave laser lines (nm): 405, 485, 514, 552 & 633.
Pulsed laser lines (nm): 440 & 470. Pulse frequency (0.3125 to 40 MHz) and intensity output is software controllable.
Tuneable spectral fluorescence detectors: 2HyD GaAsp detectors (45% QE @ 500nm) and 1PMT (30% QE @ 500 nm) are available for filter free spectral emission detection over the wavelength range from 400-800 nm in 1 nm steps. The HyD detectors can record fluorescence as a read out of photons as well as analog standard mode. A transmitted light PMT is also available for performing bright field imaging.
Fluorophores: System is ideal for exciting dyes whose excitation peak match the laser lines available e.g. DAPI, HOECHST, mEGFP, mEYFP, mVenus, Alexa Fluor 488/546/555/633/635/647, Rhodamine, Texas Red,).
Microscope: Leica DMI 6000, (invert configuration) with adjustable intensity metal halide lamp for wide field eyepiece fluorescence illumination viewing. Stage sample carrier holder available for fitting slides. Fully motorised XY stage for multi-position for tile imaging. System also has a galvanometer stage for rapid Z-focusing.
Objective lens: Air =5x, 10x, & 20x. Oil immersion = 40x & 60x.
Capabilities: FRET, FRET-FLIM, lambda spectral scan image acquisition, automated high resolution timelapse z-stack multifluorescence imaging. Motorised x-y stage can be used for multi position tile scanning. Lasers can be used to photoactivate PA-dyes or to bleach regions of interest to perform Acceptor photobleaaching FRET or FRAP experiments.