Professor Sheila Graham
- Professor of Molecular Virology (Virology)
telephone:
01413306256
email:
Sheila.Graham@glasgow.ac.uk
Centre for Virus Research, 464 Bearsden Road, Garscube Estate G61 1QH
Research interests
Publications
2024
Scott, H. , Martin, P. E. and Graham, S. V. (2024) Modulation of connexin 43 in viral infections. Tumour Virus Research, (Accepted for Publication)
Kaba, G. et al. (2024) Analysis of human papillomavirus-HPV-18 and HPV-45 type-specific variants in a contemporary cohort of individuals with cervical disease in Ghana. Ghana Medical Journal, (Accepted for Publication)
Iamborwornkun, N., Kitkumthorn, N., Stevenson, A., Kirk, A., Graham, S. V. and Chuen-im, T. (2024) Identifying regulatory elements and their RNA-binding proteins in the 3’ untranslated regions of papillomavirus late mRNAs. Biomedical Reports, 21(2), 125. (doi: 10.3892/br.2024.1813) (PMID:39006509) (PMCID:PMC11240274)
Graham, S. V. (2024) HPV and RNA binding proteins: what we know and what remains to be discovered. Viruses, 16(5), 783. (doi: 10.3390/v16050783)
White, M. P.J., Stevenson, A., Elasifer, H., Davies, C., Nomikou, K., Cuschieri, K. and Graham, S. V. (2024) Integrity of RNA in long-term-stored cervical liquid-based cytology samples: implications for biomarker research. BioTechniques, (doi: 10.2144/btn-2023-0112) (Early Online Publication)
Kirk, A. and Graham, S. (2024) The human Papillomavirus late life cycle and links to keratinocyte differentiation. Journal of Medical Virology, 96(2), e29461. (doi: 10.1002/jmv.29461) (PMID:38345171)
2023
Scott, H. , Dong, L., Stevenson, A., MacDonald, A. I., Srinivasan, S., Massimi, P., Banks, L., Martin, P. E., Johnstone, S. R. and Graham, S. V. (2023) The human Discs large protein (Dlg1) interacts with and maintains Connexin 43 at the plasma membrane in keratinocytes. Journal of Cell Science, 136(11), jcs259984. (doi: 10.1242/jcs.259984) (PMID:37288673) (PMCID:PMC10309592)
Kaba, G., Stevenson, A., Sakyi, S. A., Konney, T. O., Bhatia, R., Titiloye, N. A., Oppong, S. A., Agyemang-Yeboah, F., Cuschieri, K. and Graham, S. V. (2023) Diversity of cervicovaginal human papillomavirus (HPV) genotypes and naturally occurring E6/E7 DNA polymorphisms of HPV-16 in Ghana. Tumour Virus Research, 15, 200261. (doi: 10.1016/j.tvr.2023.200261) (PMID:37179021) (PMCID:PMC10209332)
Conley, M. J. , Epifano, I. , Kirk, A., Stevenson, A. and Graham, S. V. (2023) Microwave hyperthermia represses human papillomavirus oncoprotein activity and induces cell death due to cell stress in 3D tissue models of anogenital precancers and cancers. EBioMedicine, 91, 104577. (doi: 10.1016/j.ebiom.2023.104577) (PMID:37068348) (PMCID:PMC10130467)
2022
Guerendiain, D., Grigorescu, R., Kirk, A., Stevenson, A., Holden, M. T.G., Pan, J., Kavanagh, K., Graham, S. V. and Cuschieri, K. (2022) HPV status and HPV16 viral load in anal cancer and its association with clinical outcome. Cancer Medicine, 11(22), pp. 4193-4203. (doi: 10.1002/cam4.4771) (PMID:35785486) (PMCID:PMC9678095)
2021
Herder, V. et al. (2021) Elevated temperature inhibits SARS-CoV-2 replication in respiratory epithelium independently of IFN-mediated innate immune defences. PLoS Biology, 19(12), e3001065. (doi: 10.1371/journal.pbio.3001065) (PMID:34932557) (PMCID:PMC8765667)
Cuschieri, K., Pan, J., O Donnell, M., Kirkwood, K., Kavanagh, K., Pollock, K.G., Bhatia, R., Graham, S.V. and Wakeham, K. (2021) Penile cancer and the HPV attributable fraction in Scotland; a retrospective cohort study. Journal of Clinical Virology, 134, 104717. (doi: 10.1016/j.jcv.2020.104717) (PMID:33360857)
2020
Stevenson, A., Wakeham, K., Pan, J., Kavanagh, K., Millan, D., Bell, S., McLellan, D., Graham, S.V. and Cuschieri, K. (2020) Droplet digital PCR quantification suggests that higher viral load correlates with improved survival in HPV-positive oropharyngeal tumours. Journal of Clinical Virology, 129, 104505. (doi: 10.1016/j.jcv.2020.104505) (PMID:32604039)
Graham, S. , Mole, S., Faizo, A. A. A., Hernandez-Lopez, H., Griffiths, M. , Stevenson, A. and Roberts, S. (2020) Human papillomavirus type 16 infection activates the host serine arginine protein kinase 1 (SRPK1) - splicing factor axis. Journal of General Virology, 101(5), pp. 523-532. (doi: 10.1099/jgv.0.001402) (PMID:32182205)
2019
Wakeham, K. et al. (2019) A prospective cohort study of human papillomavirus-driven oropharyngeal cancers: implications for prognosis and immunisation. Clinical Oncology, 31(9), e132-e142. (doi: 10.1016/j.clon.2019.05.010) (PMID:31248692)
Pan, J. et al. (2019) Increased risk of HPV-associated genital cancers in men and women as a consequence of pre-invasive disease. International Journal of Cancer, 145(2), pp. 427-434. (doi: 10.1002/ijc.32126) (PMID:30650180)
Aasen, T., Leithe, E., Graham, S. V. , Kameritsch, P., Mayán, M. D., Mesnil, M., Pogoda, K. and Tabernero, A. (2019) Connexins in cancer: bridging the gap to the clinic. Oncogene, 38(23), pp. 4429-4451. (doi: 10.1038/s41388-019-0741-6) (PMID:30814684) (PMCID:PMC6555763)
Saiz-Ros, N. et al. (2019) Host vesicle fusion protein VAPB contributes to the nuclear egress stage of herpes simplex virus type-1 (HSV-1) replication. Cells, 8(2), 120. (doi: 10.3390/cells8020120)
2018
Stevenson, A., Kavanagh, K., Pan, J., Stevenson, L., Griffin, H., Doorbar, J., Scott, E., Deeny, M., Cuschieri, K. and Graham, S. V. (2018) Risk stratification of cervical disease using detection of human papillomavirus (HPV) E4 protein and cellular MCM protein in clinical liquid based cytology samples. Journal of Clinical Virology, 108, pp. 19-25. (doi: 10.1016/j.jcv.2018.08.011) (PMID:30218891) (PMCID:PMC6224362)
Graham, S. V. , Jiang, J. X. and Mesnil, M. (2018) Connexins and pannexins: important players in tumorigenesis, metastasis and potential therapeutics. International Journal of Molecular Sciences, 19(6), 1645. (doi: 10.3390/ijms19061645) (PMID:29865195)
2017
Klymenko, T. et al. (2017) RNA-Seq analysis of differentiated keratinocytes reveals a massive response to late events during human papillomavirus 16 infection, including loss of epithelial barrier function. Journal of Virology, 91(24), e01001-17. (doi: 10.1128/JVI.01001-17) (PMID:29021401) (PMCID:PMC5709591)
Graham, S. V. (2017) The human papillomavirus replication cycle, and its links to cancer progression: a comprehensive review. Clinical Science, 131(17), pp. 2201-2221. (doi: 10.1042/CS20160786) (PMID:28798073)
Graham, S. V. (2017) Keratinocyte differentiation-dependent human papillomavirus gene regulation. Viruses, 9(9), 245. (doi: 10.3390/v9090245) (PMID:28867768) (PMCID:PMC5618011)
Smith, R. W.P., Anderson, R. C., Larralde, O., Smith, J. W.S., Gorgoni, B., Richardson, W. A., Malik, P., Graham, S. V. and Gray, N. K. (2017) Viral and cellular mRNA-specific activators harness PABP and eIF4G to promote translation initiation downstream of cap binding. Proceedings of the National Academy of Sciences of the United States of America, 114(24), pp. 6310-6315. (doi: 10.1073/pnas.1610417114) (PMID:28559344) (PMCID:PMC5474791)
Graham, S. and Faizo, A. A. A. (2017) Control of human papillomavirus gene expression by alternative splicing. Virus Research, 231, pp. 83-95. (doi: 10.1016/j.virusres.2016.11.016) (PMID:27867028) (PMCID:PMC5335905)
Wakeham, K., Kavanagh, K., Cuschieri, K., Millan, D., Pollock, K. G., Bell, S., Burton, K., Reed, N. S. and Graham, S. V. (2017) HPV status and favorable outcome in vulvar squamous cancer. International Journal of Cancer, 140(5), pp. 1134-1146. (doi: 10.1002/ijc.30523) (PMID:27864932)
2016
Graham, S. V. (2016) Human papillomavirus E2 protein: linking replication, transcription, and RNA processing. Journal of Virology, 90(19), pp. 8384-8388. (doi: 10.1128/JVI.00502-16) (PMID:27412596)
Klymenko, T., Hernandez-Lopez, H., MacDonald, A.I., Bodily, J. M. and Graham, S. V. (2016) Human papillomavirus E2 regulates SRSF3 (SRp20) to promote capsid protein expression in infected differentiated keratinocytes. Journal of Virology, 90(10), pp. 5047-5058. (doi: 10.1128/JVI.03073-15) (PMID:26962216)
2015
Sun, P., Dong, L., MacDonald, A. I., Akbari, S., Edward, M., Hodgins, M. B., Johnstone, S. R. and Graham, S. V. (2015) HPV16 E6 controls the gap junction protein Cx43 in cervical tumour cells. Viruses, 7(10), pp. 5243-5256. (doi: 10.3390/v7102871) (PMID:26445057) (PMCID:PMC4632379)
McFarlane, M. , MacDonald, A. I., Stevenson, A. and Graham, S. V. (2015) Human papillomavirus type 16 oncoprotein expression is controlled by the cellular splicing factor SRSF2 (SC35). Journal of Virology, 89(10), pp. 5276-5287. (doi: 10.1128/JVI.03434-14) (PMID:25717103)
2014
Prescott, E. L., Brimacombe, C. L., Hartley, M., Bell, I., Graham, S. and Roberts, S. (2014) Human papillomavirus type 1 E1^E4 protein is a potent inhibitor of the Serine-Arginine (SR) protein kinase SRPK1 and inhibits phosphorylation of host SR proteins and of the viral transcription and replication regulator E2. Journal of Virology, 88(21), pp. 12599-12611. (doi: 10.1128/JVI.02029-14)
Chambers, G., Millan, D., Cuschieri, K., Cubie, H.A. and Graham, S.V. (2014) Assessing the detection of human papillomavirus late mRNA in liquid base cytology samples for risk stratification of cervical disease. Journal of Medical Virology, 86(4), pp. 627-633. (doi: 10.1002/jmv.23793) (PMID:24142394) (PMCID:PMC4282440)
2013
Braidwood, L., Graham, S.V. , Graham, A. and Conner, J. (2013) Oncolytic herpes viruses, chemotherapeutics, and other cancer drugs. Oncolytic Virotherapy, 2, pp. 57-74. (doi: 10.2147/OV.S52601)
McCusker, S.M., Macqueen, I., Lough, G., MacDonald, A.I., Campbell, C. and Graham, S.V. (2013) Gaps in detailed knowledge of human papillomavirus (HPV) and the HPV vaccine among medical students in Scotland. BMC Public Health, 13(264), (doi: 10.1186/1471-2458-13-264)
2012
Malik, P., Tabarraei, A., Kehlenbach, R. H., Korfali, N., Iwasawa, R., Graham, S. and Schirmer, E. C. (2012) Herpes simplex virus ICP27 protein directly interacts with the nuclear pore complex through NUP62, inhibiting host nucleocytoplasmic transport pathways. Journal of Biological Chemistry, 287(15), pp. 12277-12292. (doi: 10.1074/jbc.M111.331777)
Hernandez-Lopez, H. and Graham, S. (2012) Alternative splicing in tumour viruses: a therapeutic target? Biochemical Journal, 445, pp. 145-156. (doi: 10.1042/BJ20120413) (PMID:22738337)
Hernandez-Lopez, H.R. and Graham, S.V. (2012) Alternative splicing in human tumour viruses: a therapeutic target? Biochemical Journal, 445(2), pp. 145-156.
Klymenko, T. and Graham, S. (2012) Human papillomavirus gene expression is controlled by host cell splicing factors. Biochemical Society Transactions, 40, pp. 773-777. (doi: 10.1042/BST20120079) (PMID:22817732)
MacDonald, A.I. et al. (2012) A functional interaction between the MAGUK protein hDlg and the gap junction protein Connexin 43 in cervical tumour cells. Biochemical Journal, 446(1), pp. 9-21. (doi: 10.1042/BJ20111144)
2011
Burgess, H. M., Richardson, W. A., Anderson, R. C., Salaun, C., Graham, S. V. and Gray, N. K. (2011) Nuclear relocalisation of cytoplasmic poly(A)-binding proteins PABP1 and PABP4 in response to UV irradiation reveals mRNA-dependent export of metazoan PABPs. Journal of Cell Science, 124(19), pp. 3344-3355. (doi: 10.1242/jcs.087692) (PMID:21940797) (PMCID:PMC3178455)
2010
Salaun, C., MacDonald, A.I., Larralde, O., Howard, L., Lochtie, K., Burgess, H.M., Brook, M., Malik, P., Gray, N.K. and Graham, S.V. (2010) Poly(A)-binding protein 1 (PABP1) partially relocalises to the nucleus during HSV-1 infection in an ICP27-independent manner and does not inhibit virus replication. Journal of Virology, 84(17), pp. 8539-8548. (doi: 10.1128/JVI.00668-10) (PMID:20573819) (PMCID:PMC2919032)
Salaun, C., MacDonald, A.I., Larralde, O., Howard, L., Lochtie, K., Burgess, H.M., Brook, M., Malik, P., Gray, N.K. and Graham, S.V. (2010) Poly(A)-binding protein 1 partially relocalizes to the nucleus during herpes simplex virus type 1 infection in an ICP27-independent manner and does not inhibit virus replication. Journal of Virology, 84(17), pp. 8539-8548. (doi: 10.1128/JVI.00668-10)
Chuen-Im, T., Areekijseree, M., Chongthammakun, S. and Graham, S.V. (2010) Aerobic bacterial infections in captive juvenile green turtles (Chelonia mydas) and hawksbill turtles (Eretmochelys imbricata) from Thailand. Chelonian Conservation and Biology, 9(1), pp. 135-142. (doi: 10.2744/CCB-0808.1)
Barbaresi, S., Cortese, M. S., Quinn, J., Ashrafi, G. H., Graham, S.V. and Campo, M.S. (2010) Effects of human papillomavirus type 16 E5 deletion mutants on epithelial morphology: functional characterization of each transmembrane domain. Journal of General Virology, 91(2), pp. 521-530. (doi: 10.1099/vir.0.016295-0)
Campo, M.S., Graham, S.V. , Cortese, M.S., Ashrafi, G.H., Araibi, E.H., Dornan, E.S., Miners, K., Nunes, C. and Man, S. (2010) HPV-16 E5 down-regulates expression of surface HLA class I and reduces recognition by CD8 T cells. Virology, 407(1), pp. 137-142. (doi: 10.1016/j.virol.2010.07.044)
Chuen-Im, T., Areekijseree, M., Chongthammakun, S. and Graham, S.V. (2010) Aerobic Bacterial Infections in Captive Juvenile Green Turtles (Chelonia mydas) and Hawksbill Turtles (Eretmochelys imbricata) from Thailand. Chelonian Conservation and Biology, 9(1), pp. 135-142. (doi: 10.2744/CCB-0808.1)
Graham, S.V. (2010) Human papillomavirus: gene expression, regulation and prospects for novel diagnostic methods and antiviral therapies. Future Microbiology, 5(10), pp. 1493-1506. (doi: 10.2217/fmb.10.107)
McFarlane, M. and Graham, S.V. (2010) Human papillomavirus regulation of SR proteins. Biochemical Society Transactions, 38(4), pp. 1116-1121. (doi: 10.1042/BST0381116)
2009
Johansson, C., Graham, S.V. , Dornan, E.S. and Morgan, I.M. (2009) The human papillomavirus 16 E2 protein is stabilised in S phase. Virology, 394(2), pp. 194-199. (doi: 10.1016/j.virol.2009.08.046) (PMID:19781729)
Mole, S., McFarlane, M. , Chuen-Im, T., Milligan, S.G., Millan, D. and Graham, S.V. (2009) RNA splicing factors regulated by HPV16 during cervical tumour progression. Journal of Pathology, 219(3), pp. 383-391. (doi: 10.1002/path.2608) (PMID:19718710) (PMCID:PMC2779514)
Cumming, S.A., Chuen-Im, T., Zhang, J. and Graham, S.V. (2009) The RNA stability regulator HuR regulates L1 protein expression in vivo in differentiating cervical epithelial cells. Virology, 383(1), pp. 142-149. (doi: 10.1016/j.virol.2008.10.003)
Cumming, S.A., Chuen-Im, T., Zhang, J. and Graham, S.V. (2009) The RNA stability regulator HuR regulates L1 protein expression in vivo in differentiating cervical epithelial cells. Virology, 383(1), pp. 142-149. (doi: 10.1016/j.virol.2008.%U 10.003)
Mole, S., Milligan, S.G. and Graham, S.V. (2009) Human Papillomavirus Type 16 E2 Protein Transcriptionally Activates the Promoter of a Key Cellular Splicing Factor, SF2/ASF. Journal of Virology, 83(1), pp. 357-367. (doi: 10.1128/JVI.01414-08) (PMID:18945764) (PMCID:PMC2612322)
2008
Smith, R. W.P., Graham, S. V. and Gray, N. K. (2008) Regulation of translation initiation by herpesviruses. Biochemical Society Transactions, 36(4), pp. 701-707. (doi: 10.1042/BST0360701)
Cheunim, T., Zhang, J., Milligan, S.G., McPhillips, M.G. and Graham, S.V. (2008) The alternative splicing factor hnRNP A1 is up-regulated during virus-infected epithelial cell differentiation and binds the human papillomavirus type 16 late regulatory element. Virus Research, 131(2), pp. 189-198. (doi: 10.1016/j.virusres.2007.09.006)
Cumming, S. A., Cheun-Im, T., Milligan, S. G. and Graham, S. V. (2008) Human papillomavirus type 16 late gene expression is regulated by cellular RNA processing factors in response to epithelial differentiation. Biochemical Society Transactions, 36(3), pp. 522-524. (doi: 10.1042/BST0360522)
Graham, S.V. (2008) Papillomavirus 3' UTR regulatory elements. Frontiers in Bioscience, 13(1), pp. 5646-5663. (doi: 10.2741/3107)
2007
Milligan, S., Veerapraditsin, T., Ahamet, B., Mole, S. and Graham, S. (2007) Analysis of novel human papillomavirus type 16 late mRNAs in differentiated W12 cervical epithelial cells. Virology, 360, pp. 172-181. (doi: 10.1016/j.virol.2006.10.012)
2006
Mole, S., Veerapraditsin, T., McPhillips, M. and Graham, S. (2006) Regulation of splicing-associated SR proteins by HPV-16. Biochemical Society Transactions, 34, pp. 1145-1147.
2005
Aasen, T., Edward, M., Graham, S. and Hodgins, M. (2005) Reduced expression of multiple gap junction proteins is a feature of cervical dysplasia. Molecular Cancer, 4, (doi: 10.1186/1476-4598-4-31)
Daly, N.L., Arvanitis, D.A., Fairley, J.A., Gomez-Roman, N. , Morton, J.P. , Graham, S.V., Spandidos, D.A. and White, R.J. (2005) Deregulation of RNA polymerase III transcription in cervical epithelium in response to high-risk human papillomavirus. Oncogene, 24, pp. 880-888. (doi: 10.1038/sj.onc.1208031)
Graham, S. (2005) Analysis of regulatory motifs within HPV transcripts. Methods in Molecular Medicine, 119(.), pp. 291-315.
2004
McPhillips, M. G., Veerapraditsin, T., Cumming, S. A., Karali, D., Milligan, S. G., Boner, W. , Morgan, I. M. and Graham, S. V. (2004) SF2/ASF binds the human papillomavirus type 16 late RNA control element and is regulated during differentiation of virus-infected epithelial cells. Journal of Virology, 78, pp. 10598-10605. (doi: 10.1128/JVI.78.19.10598-10605.2004) (PMID:15367627) (PMCID:PMC516382)
2003
Aasen, T., Edward, M., Graham, S. and Hodgins, M. (2003) The relationship between connexins, gap junctions, tissue architecture and tumour invasion, as studied in a novel in vitro model of HPV-16-associated cervical cancer progression. Oncogene, 22, pp. 7969-7980. (doi: 10.1038/sj.onc.1206709)
Cumming, S., McPhillips, M., Veerapraditsin, T., Milligan, S. and Graham, S. (2003) Activity of the human papillomavirus type 16 late negative regulatory element is partly due to four weak consensus 5 ' splice sites that bind a U1 snRNP-like complex. Journal of Virology, 77, pp. 5167-5177. (doi: 10.1128/JVI.77.9.5167-5177.2003)
Graham, S. (2003) Nonsense-mediated decay breaks the circle? Biochemical Journal, 373(Pt 3), e5-e6.
2002
Attrill, H., Cumming, S., Clements, J. and Graham, S. (2002) The herpes simplex virus type 1 US11 protein binds the coterminal UL12, UL13, and UL14 RNAs and regulates UL13 expression in vivo. Journal of Virology, 76, pp. 8090-8100. (doi: 10.1128/JVI.76.16.8090-8100.2002)
Cumming, S., Repellin, C., McPhillips, M., Radford, J., Clements, J. and Graham, S. (2002) The human papillomavirus type 31 late 3 ' untranslated region contains a complex bipartite negative regulatory element. Journal of Virology, 76, pp. 5993-6003. (doi: 10.1128/JVI.76.12.5993-6003.2002)
2001
Adamson, R., Lyons, K., Sharrard, M., Kinnaird, J., Swan, D., Graham, S., Shiels, B. and Hall, R. (2001) Transient transfection of Theileria annulata. Molecular and Biochemical Parasitology, 114, pp. 53-61. (PMID:11356513)
Articles
Scott, H. , Martin, P. E. and Graham, S. V. (2024) Modulation of connexin 43 in viral infections. Tumour Virus Research, (Accepted for Publication)
Kaba, G. et al. (2024) Analysis of human papillomavirus-HPV-18 and HPV-45 type-specific variants in a contemporary cohort of individuals with cervical disease in Ghana. Ghana Medical Journal, (Accepted for Publication)
Iamborwornkun, N., Kitkumthorn, N., Stevenson, A., Kirk, A., Graham, S. V. and Chuen-im, T. (2024) Identifying regulatory elements and their RNA-binding proteins in the 3’ untranslated regions of papillomavirus late mRNAs. Biomedical Reports, 21(2), 125. (doi: 10.3892/br.2024.1813) (PMID:39006509) (PMCID:PMC11240274)
Graham, S. V. (2024) HPV and RNA binding proteins: what we know and what remains to be discovered. Viruses, 16(5), 783. (doi: 10.3390/v16050783)
White, M. P.J., Stevenson, A., Elasifer, H., Davies, C., Nomikou, K., Cuschieri, K. and Graham, S. V. (2024) Integrity of RNA in long-term-stored cervical liquid-based cytology samples: implications for biomarker research. BioTechniques, (doi: 10.2144/btn-2023-0112) (Early Online Publication)
Kirk, A. and Graham, S. (2024) The human Papillomavirus late life cycle and links to keratinocyte differentiation. Journal of Medical Virology, 96(2), e29461. (doi: 10.1002/jmv.29461) (PMID:38345171)
Scott, H. , Dong, L., Stevenson, A., MacDonald, A. I., Srinivasan, S., Massimi, P., Banks, L., Martin, P. E., Johnstone, S. R. and Graham, S. V. (2023) The human Discs large protein (Dlg1) interacts with and maintains Connexin 43 at the plasma membrane in keratinocytes. Journal of Cell Science, 136(11), jcs259984. (doi: 10.1242/jcs.259984) (PMID:37288673) (PMCID:PMC10309592)
Kaba, G., Stevenson, A., Sakyi, S. A., Konney, T. O., Bhatia, R., Titiloye, N. A., Oppong, S. A., Agyemang-Yeboah, F., Cuschieri, K. and Graham, S. V. (2023) Diversity of cervicovaginal human papillomavirus (HPV) genotypes and naturally occurring E6/E7 DNA polymorphisms of HPV-16 in Ghana. Tumour Virus Research, 15, 200261. (doi: 10.1016/j.tvr.2023.200261) (PMID:37179021) (PMCID:PMC10209332)
Conley, M. J. , Epifano, I. , Kirk, A., Stevenson, A. and Graham, S. V. (2023) Microwave hyperthermia represses human papillomavirus oncoprotein activity and induces cell death due to cell stress in 3D tissue models of anogenital precancers and cancers. EBioMedicine, 91, 104577. (doi: 10.1016/j.ebiom.2023.104577) (PMID:37068348) (PMCID:PMC10130467)
Guerendiain, D., Grigorescu, R., Kirk, A., Stevenson, A., Holden, M. T.G., Pan, J., Kavanagh, K., Graham, S. V. and Cuschieri, K. (2022) HPV status and HPV16 viral load in anal cancer and its association with clinical outcome. Cancer Medicine, 11(22), pp. 4193-4203. (doi: 10.1002/cam4.4771) (PMID:35785486) (PMCID:PMC9678095)
Herder, V. et al. (2021) Elevated temperature inhibits SARS-CoV-2 replication in respiratory epithelium independently of IFN-mediated innate immune defences. PLoS Biology, 19(12), e3001065. (doi: 10.1371/journal.pbio.3001065) (PMID:34932557) (PMCID:PMC8765667)
Cuschieri, K., Pan, J., O Donnell, M., Kirkwood, K., Kavanagh, K., Pollock, K.G., Bhatia, R., Graham, S.V. and Wakeham, K. (2021) Penile cancer and the HPV attributable fraction in Scotland; a retrospective cohort study. Journal of Clinical Virology, 134, 104717. (doi: 10.1016/j.jcv.2020.104717) (PMID:33360857)
Stevenson, A., Wakeham, K., Pan, J., Kavanagh, K., Millan, D., Bell, S., McLellan, D., Graham, S.V. and Cuschieri, K. (2020) Droplet digital PCR quantification suggests that higher viral load correlates with improved survival in HPV-positive oropharyngeal tumours. Journal of Clinical Virology, 129, 104505. (doi: 10.1016/j.jcv.2020.104505) (PMID:32604039)
Graham, S. , Mole, S., Faizo, A. A. A., Hernandez-Lopez, H., Griffiths, M. , Stevenson, A. and Roberts, S. (2020) Human papillomavirus type 16 infection activates the host serine arginine protein kinase 1 (SRPK1) - splicing factor axis. Journal of General Virology, 101(5), pp. 523-532. (doi: 10.1099/jgv.0.001402) (PMID:32182205)
Wakeham, K. et al. (2019) A prospective cohort study of human papillomavirus-driven oropharyngeal cancers: implications for prognosis and immunisation. Clinical Oncology, 31(9), e132-e142. (doi: 10.1016/j.clon.2019.05.010) (PMID:31248692)
Pan, J. et al. (2019) Increased risk of HPV-associated genital cancers in men and women as a consequence of pre-invasive disease. International Journal of Cancer, 145(2), pp. 427-434. (doi: 10.1002/ijc.32126) (PMID:30650180)
Aasen, T., Leithe, E., Graham, S. V. , Kameritsch, P., Mayán, M. D., Mesnil, M., Pogoda, K. and Tabernero, A. (2019) Connexins in cancer: bridging the gap to the clinic. Oncogene, 38(23), pp. 4429-4451. (doi: 10.1038/s41388-019-0741-6) (PMID:30814684) (PMCID:PMC6555763)
Saiz-Ros, N. et al. (2019) Host vesicle fusion protein VAPB contributes to the nuclear egress stage of herpes simplex virus type-1 (HSV-1) replication. Cells, 8(2), 120. (doi: 10.3390/cells8020120)
Stevenson, A., Kavanagh, K., Pan, J., Stevenson, L., Griffin, H., Doorbar, J., Scott, E., Deeny, M., Cuschieri, K. and Graham, S. V. (2018) Risk stratification of cervical disease using detection of human papillomavirus (HPV) E4 protein and cellular MCM protein in clinical liquid based cytology samples. Journal of Clinical Virology, 108, pp. 19-25. (doi: 10.1016/j.jcv.2018.08.011) (PMID:30218891) (PMCID:PMC6224362)
Graham, S. V. , Jiang, J. X. and Mesnil, M. (2018) Connexins and pannexins: important players in tumorigenesis, metastasis and potential therapeutics. International Journal of Molecular Sciences, 19(6), 1645. (doi: 10.3390/ijms19061645) (PMID:29865195)
Klymenko, T. et al. (2017) RNA-Seq analysis of differentiated keratinocytes reveals a massive response to late events during human papillomavirus 16 infection, including loss of epithelial barrier function. Journal of Virology, 91(24), e01001-17. (doi: 10.1128/JVI.01001-17) (PMID:29021401) (PMCID:PMC5709591)
Graham, S. V. (2017) The human papillomavirus replication cycle, and its links to cancer progression: a comprehensive review. Clinical Science, 131(17), pp. 2201-2221. (doi: 10.1042/CS20160786) (PMID:28798073)
Graham, S. V. (2017) Keratinocyte differentiation-dependent human papillomavirus gene regulation. Viruses, 9(9), 245. (doi: 10.3390/v9090245) (PMID:28867768) (PMCID:PMC5618011)
Smith, R. W.P., Anderson, R. C., Larralde, O., Smith, J. W.S., Gorgoni, B., Richardson, W. A., Malik, P., Graham, S. V. and Gray, N. K. (2017) Viral and cellular mRNA-specific activators harness PABP and eIF4G to promote translation initiation downstream of cap binding. Proceedings of the National Academy of Sciences of the United States of America, 114(24), pp. 6310-6315. (doi: 10.1073/pnas.1610417114) (PMID:28559344) (PMCID:PMC5474791)
Graham, S. and Faizo, A. A. A. (2017) Control of human papillomavirus gene expression by alternative splicing. Virus Research, 231, pp. 83-95. (doi: 10.1016/j.virusres.2016.11.016) (PMID:27867028) (PMCID:PMC5335905)
Wakeham, K., Kavanagh, K., Cuschieri, K., Millan, D., Pollock, K. G., Bell, S., Burton, K., Reed, N. S. and Graham, S. V. (2017) HPV status and favorable outcome in vulvar squamous cancer. International Journal of Cancer, 140(5), pp. 1134-1146. (doi: 10.1002/ijc.30523) (PMID:27864932)
Graham, S. V. (2016) Human papillomavirus E2 protein: linking replication, transcription, and RNA processing. Journal of Virology, 90(19), pp. 8384-8388. (doi: 10.1128/JVI.00502-16) (PMID:27412596)
Klymenko, T., Hernandez-Lopez, H., MacDonald, A.I., Bodily, J. M. and Graham, S. V. (2016) Human papillomavirus E2 regulates SRSF3 (SRp20) to promote capsid protein expression in infected differentiated keratinocytes. Journal of Virology, 90(10), pp. 5047-5058. (doi: 10.1128/JVI.03073-15) (PMID:26962216)
Sun, P., Dong, L., MacDonald, A. I., Akbari, S., Edward, M., Hodgins, M. B., Johnstone, S. R. and Graham, S. V. (2015) HPV16 E6 controls the gap junction protein Cx43 in cervical tumour cells. Viruses, 7(10), pp. 5243-5256. (doi: 10.3390/v7102871) (PMID:26445057) (PMCID:PMC4632379)
McFarlane, M. , MacDonald, A. I., Stevenson, A. and Graham, S. V. (2015) Human papillomavirus type 16 oncoprotein expression is controlled by the cellular splicing factor SRSF2 (SC35). Journal of Virology, 89(10), pp. 5276-5287. (doi: 10.1128/JVI.03434-14) (PMID:25717103)
Prescott, E. L., Brimacombe, C. L., Hartley, M., Bell, I., Graham, S. and Roberts, S. (2014) Human papillomavirus type 1 E1^E4 protein is a potent inhibitor of the Serine-Arginine (SR) protein kinase SRPK1 and inhibits phosphorylation of host SR proteins and of the viral transcription and replication regulator E2. Journal of Virology, 88(21), pp. 12599-12611. (doi: 10.1128/JVI.02029-14)
Chambers, G., Millan, D., Cuschieri, K., Cubie, H.A. and Graham, S.V. (2014) Assessing the detection of human papillomavirus late mRNA in liquid base cytology samples for risk stratification of cervical disease. Journal of Medical Virology, 86(4), pp. 627-633. (doi: 10.1002/jmv.23793) (PMID:24142394) (PMCID:PMC4282440)
Braidwood, L., Graham, S.V. , Graham, A. and Conner, J. (2013) Oncolytic herpes viruses, chemotherapeutics, and other cancer drugs. Oncolytic Virotherapy, 2, pp. 57-74. (doi: 10.2147/OV.S52601)
McCusker, S.M., Macqueen, I., Lough, G., MacDonald, A.I., Campbell, C. and Graham, S.V. (2013) Gaps in detailed knowledge of human papillomavirus (HPV) and the HPV vaccine among medical students in Scotland. BMC Public Health, 13(264), (doi: 10.1186/1471-2458-13-264)
Malik, P., Tabarraei, A., Kehlenbach, R. H., Korfali, N., Iwasawa, R., Graham, S. and Schirmer, E. C. (2012) Herpes simplex virus ICP27 protein directly interacts with the nuclear pore complex through NUP62, inhibiting host nucleocytoplasmic transport pathways. Journal of Biological Chemistry, 287(15), pp. 12277-12292. (doi: 10.1074/jbc.M111.331777)
Hernandez-Lopez, H. and Graham, S. (2012) Alternative splicing in tumour viruses: a therapeutic target? Biochemical Journal, 445, pp. 145-156. (doi: 10.1042/BJ20120413) (PMID:22738337)
Hernandez-Lopez, H.R. and Graham, S.V. (2012) Alternative splicing in human tumour viruses: a therapeutic target? Biochemical Journal, 445(2), pp. 145-156.
Klymenko, T. and Graham, S. (2012) Human papillomavirus gene expression is controlled by host cell splicing factors. Biochemical Society Transactions, 40, pp. 773-777. (doi: 10.1042/BST20120079) (PMID:22817732)
MacDonald, A.I. et al. (2012) A functional interaction between the MAGUK protein hDlg and the gap junction protein Connexin 43 in cervical tumour cells. Biochemical Journal, 446(1), pp. 9-21. (doi: 10.1042/BJ20111144)
Burgess, H. M., Richardson, W. A., Anderson, R. C., Salaun, C., Graham, S. V. and Gray, N. K. (2011) Nuclear relocalisation of cytoplasmic poly(A)-binding proteins PABP1 and PABP4 in response to UV irradiation reveals mRNA-dependent export of metazoan PABPs. Journal of Cell Science, 124(19), pp. 3344-3355. (doi: 10.1242/jcs.087692) (PMID:21940797) (PMCID:PMC3178455)
Salaun, C., MacDonald, A.I., Larralde, O., Howard, L., Lochtie, K., Burgess, H.M., Brook, M., Malik, P., Gray, N.K. and Graham, S.V. (2010) Poly(A)-binding protein 1 (PABP1) partially relocalises to the nucleus during HSV-1 infection in an ICP27-independent manner and does not inhibit virus replication. Journal of Virology, 84(17), pp. 8539-8548. (doi: 10.1128/JVI.00668-10) (PMID:20573819) (PMCID:PMC2919032)
Salaun, C., MacDonald, A.I., Larralde, O., Howard, L., Lochtie, K., Burgess, H.M., Brook, M., Malik, P., Gray, N.K. and Graham, S.V. (2010) Poly(A)-binding protein 1 partially relocalizes to the nucleus during herpes simplex virus type 1 infection in an ICP27-independent manner and does not inhibit virus replication. Journal of Virology, 84(17), pp. 8539-8548. (doi: 10.1128/JVI.00668-10)
Chuen-Im, T., Areekijseree, M., Chongthammakun, S. and Graham, S.V. (2010) Aerobic bacterial infections in captive juvenile green turtles (Chelonia mydas) and hawksbill turtles (Eretmochelys imbricata) from Thailand. Chelonian Conservation and Biology, 9(1), pp. 135-142. (doi: 10.2744/CCB-0808.1)
Barbaresi, S., Cortese, M. S., Quinn, J., Ashrafi, G. H., Graham, S.V. and Campo, M.S. (2010) Effects of human papillomavirus type 16 E5 deletion mutants on epithelial morphology: functional characterization of each transmembrane domain. Journal of General Virology, 91(2), pp. 521-530. (doi: 10.1099/vir.0.016295-0)
Campo, M.S., Graham, S.V. , Cortese, M.S., Ashrafi, G.H., Araibi, E.H., Dornan, E.S., Miners, K., Nunes, C. and Man, S. (2010) HPV-16 E5 down-regulates expression of surface HLA class I and reduces recognition by CD8 T cells. Virology, 407(1), pp. 137-142. (doi: 10.1016/j.virol.2010.07.044)
Chuen-Im, T., Areekijseree, M., Chongthammakun, S. and Graham, S.V. (2010) Aerobic Bacterial Infections in Captive Juvenile Green Turtles (Chelonia mydas) and Hawksbill Turtles (Eretmochelys imbricata) from Thailand. Chelonian Conservation and Biology, 9(1), pp. 135-142. (doi: 10.2744/CCB-0808.1)
Graham, S.V. (2010) Human papillomavirus: gene expression, regulation and prospects for novel diagnostic methods and antiviral therapies. Future Microbiology, 5(10), pp. 1493-1506. (doi: 10.2217/fmb.10.107)
McFarlane, M. and Graham, S.V. (2010) Human papillomavirus regulation of SR proteins. Biochemical Society Transactions, 38(4), pp. 1116-1121. (doi: 10.1042/BST0381116)
Johansson, C., Graham, S.V. , Dornan, E.S. and Morgan, I.M. (2009) The human papillomavirus 16 E2 protein is stabilised in S phase. Virology, 394(2), pp. 194-199. (doi: 10.1016/j.virol.2009.08.046) (PMID:19781729)
Mole, S., McFarlane, M. , Chuen-Im, T., Milligan, S.G., Millan, D. and Graham, S.V. (2009) RNA splicing factors regulated by HPV16 during cervical tumour progression. Journal of Pathology, 219(3), pp. 383-391. (doi: 10.1002/path.2608) (PMID:19718710) (PMCID:PMC2779514)
Cumming, S.A., Chuen-Im, T., Zhang, J. and Graham, S.V. (2009) The RNA stability regulator HuR regulates L1 protein expression in vivo in differentiating cervical epithelial cells. Virology, 383(1), pp. 142-149. (doi: 10.1016/j.virol.2008.10.003)
Cumming, S.A., Chuen-Im, T., Zhang, J. and Graham, S.V. (2009) The RNA stability regulator HuR regulates L1 protein expression in vivo in differentiating cervical epithelial cells. Virology, 383(1), pp. 142-149. (doi: 10.1016/j.virol.2008.%U 10.003)
Mole, S., Milligan, S.G. and Graham, S.V. (2009) Human Papillomavirus Type 16 E2 Protein Transcriptionally Activates the Promoter of a Key Cellular Splicing Factor, SF2/ASF. Journal of Virology, 83(1), pp. 357-367. (doi: 10.1128/JVI.01414-08) (PMID:18945764) (PMCID:PMC2612322)
Smith, R. W.P., Graham, S. V. and Gray, N. K. (2008) Regulation of translation initiation by herpesviruses. Biochemical Society Transactions, 36(4), pp. 701-707. (doi: 10.1042/BST0360701)
Cheunim, T., Zhang, J., Milligan, S.G., McPhillips, M.G. and Graham, S.V. (2008) The alternative splicing factor hnRNP A1 is up-regulated during virus-infected epithelial cell differentiation and binds the human papillomavirus type 16 late regulatory element. Virus Research, 131(2), pp. 189-198. (doi: 10.1016/j.virusres.2007.09.006)
Cumming, S. A., Cheun-Im, T., Milligan, S. G. and Graham, S. V. (2008) Human papillomavirus type 16 late gene expression is regulated by cellular RNA processing factors in response to epithelial differentiation. Biochemical Society Transactions, 36(3), pp. 522-524. (doi: 10.1042/BST0360522)
Graham, S.V. (2008) Papillomavirus 3' UTR regulatory elements. Frontiers in Bioscience, 13(1), pp. 5646-5663. (doi: 10.2741/3107)
Milligan, S., Veerapraditsin, T., Ahamet, B., Mole, S. and Graham, S. (2007) Analysis of novel human papillomavirus type 16 late mRNAs in differentiated W12 cervical epithelial cells. Virology, 360, pp. 172-181. (doi: 10.1016/j.virol.2006.10.012)
Mole, S., Veerapraditsin, T., McPhillips, M. and Graham, S. (2006) Regulation of splicing-associated SR proteins by HPV-16. Biochemical Society Transactions, 34, pp. 1145-1147.
Aasen, T., Edward, M., Graham, S. and Hodgins, M. (2005) Reduced expression of multiple gap junction proteins is a feature of cervical dysplasia. Molecular Cancer, 4, (doi: 10.1186/1476-4598-4-31)
Daly, N.L., Arvanitis, D.A., Fairley, J.A., Gomez-Roman, N. , Morton, J.P. , Graham, S.V., Spandidos, D.A. and White, R.J. (2005) Deregulation of RNA polymerase III transcription in cervical epithelium in response to high-risk human papillomavirus. Oncogene, 24, pp. 880-888. (doi: 10.1038/sj.onc.1208031)
Graham, S. (2005) Analysis of regulatory motifs within HPV transcripts. Methods in Molecular Medicine, 119(.), pp. 291-315.
McPhillips, M. G., Veerapraditsin, T., Cumming, S. A., Karali, D., Milligan, S. G., Boner, W. , Morgan, I. M. and Graham, S. V. (2004) SF2/ASF binds the human papillomavirus type 16 late RNA control element and is regulated during differentiation of virus-infected epithelial cells. Journal of Virology, 78, pp. 10598-10605. (doi: 10.1128/JVI.78.19.10598-10605.2004) (PMID:15367627) (PMCID:PMC516382)
Aasen, T., Edward, M., Graham, S. and Hodgins, M. (2003) The relationship between connexins, gap junctions, tissue architecture and tumour invasion, as studied in a novel in vitro model of HPV-16-associated cervical cancer progression. Oncogene, 22, pp. 7969-7980. (doi: 10.1038/sj.onc.1206709)
Cumming, S., McPhillips, M., Veerapraditsin, T., Milligan, S. and Graham, S. (2003) Activity of the human papillomavirus type 16 late negative regulatory element is partly due to four weak consensus 5 ' splice sites that bind a U1 snRNP-like complex. Journal of Virology, 77, pp. 5167-5177. (doi: 10.1128/JVI.77.9.5167-5177.2003)
Graham, S. (2003) Nonsense-mediated decay breaks the circle? Biochemical Journal, 373(Pt 3), e5-e6.
Attrill, H., Cumming, S., Clements, J. and Graham, S. (2002) The herpes simplex virus type 1 US11 protein binds the coterminal UL12, UL13, and UL14 RNAs and regulates UL13 expression in vivo. Journal of Virology, 76, pp. 8090-8100. (doi: 10.1128/JVI.76.16.8090-8100.2002)
Cumming, S., Repellin, C., McPhillips, M., Radford, J., Clements, J. and Graham, S. (2002) The human papillomavirus type 31 late 3 ' untranslated region contains a complex bipartite negative regulatory element. Journal of Virology, 76, pp. 5993-6003. (doi: 10.1128/JVI.76.12.5993-6003.2002)
Adamson, R., Lyons, K., Sharrard, M., Kinnaird, J., Swan, D., Graham, S., Shiels, B. and Hall, R. (2001) Transient transfection of Theileria annulata. Molecular and Biochemical Parasitology, 114, pp. 53-61. (PMID:11356513)
Grants
Grants and Awards listed are those received whilst working with the University of Glasgow.
- Risk stratification of HPV in self taken vaginal samples - a potential molecular solution to screening
Office of the Chief Scientific Adviser
2023 - 2025
- BioMARSC: RNA Biomarker Matrix for Risk Stratification of Cervical Disease
Medical Research Council
2022 - 2022
- MiTAG- Microwave treatment for anogenital disease
Chief Scientist Office
2019 - 2021
- MiTAG- Microwave treatment for anogenital disease
Office of the Chief Scientific Adviser
2019 - 2022
- Microwaves destroying HPV infection as a new therapy against HPV-associated diseases
Innovate UK
2017 - 2018
- Quinquennial Core Funds
Medical Research Council
2016 - 2021
- A three dimensional tissue model for examining the mechanism of oncolytic herpes virus activity and synergy with chemotherapeutic agents in epithelial tumour cells.
Medical Research Scotland
2015 - 2019
- Confidence in Concept: Biomarker development for use in liquid based cytology samples
Medical Research Council
2014 - 2015
- Cell transcriptome profiling of cervical smear samples in search for correlation between cellular gene expression signatures and cervical disease stage
Tenovus Scotland
2012 - 2012
- Centre for Virus Research
Medical Research Council
2010 - 2016
- Human papillomavirus E2 regulation of splicing factors: consequences for the virus life cycle
Wellcome Trust
2010 - 2012
- Centre for Integrated Virology
Medical Research Council
2010 - 2015
- Investigating high risk HPV late mRNAs present in tissues isolated from patients with cervical lesions for development of new diagnostic procedures
Scottish Executive Health Department
2009 - 2010
- The role of the hD1g, ZO-1 and hScrib tumour suppressors in control of Cx43 gap junctional intercellular communication in HPV-associated tumourigenesis.
Association for International Cancer Research
2008 - 2011
- TopBP1 as therapeutic target for human papillomavirus infection.
Medical Research Scotland
2007 - 2011
- A therapeutic strategy for improving treatment of virally induced gynaecological cancers and pre-maglignant tumours
Wellbeing
2006 - 2008
- A family of multifunctional proteins that orchestrates RNA metabolism in herpesvirus infected cells
Medical Research Council
2005 - 2008
- Confocal Microscope
Wellcome Trust
2002 - 2007
- Dissecting HPV-16 late gene expression during epithelial cell differentiation
Biotechnology and Biological Sciences Research Council
2002 - 2005
Additional information
Editorial Board
- 2005 - 2008: Biochemical Society Transactions - Advisory Board
- 1999 - present: Biochemical Journal - Deputy Chair from end of 2004
Invited International Presentations
- 2010: Madison, Wisconsin, USA - Invited Chair at the 2010 Molecular Biology of DNA Tumour Viruses Conference
- 2009: Glasgow, UK - Invited Chair at the 13th Tenovus Symposium
- 2009: Oxford, UK - Invited Chair at DNA Tumour Virus Meeting
- 2008: Edinburgh, Scotland - Invited speaker Virus Group Symposium
- 2007: Glasgow, Scotland - Member of the Scientific Programme Committee for LifeSciences 2007
- 2007: Manchester, England - Organiser of the workshop on “Tumour viruses” Society for General Microbiology, Spring Meeting
- 2007: Manchester, England - Chair, Virus Group Symposium "Molecular Aspects of Virus Replication From Entry Through to Exit". Society for General Microbiology, Spring Meeting
- 2006: University of Warwick, UK - Society for General Microbiology Spring Meeting 2006 Chair, "Virus interactions with subcellular structures"
- 2005: University of Uppsala, Sweden - "Regulation of HPV16 late gene expression in response to epithelial differentiation."
- 2004: University of Edinburgh, UK - Chair, Splicing Session 1 RNA Structure and Function- a joint meeting of the Biochemical Society and the Royal Society of Chemistry
- 2004: University of Bath, UK - Chair, UK Papillomavirus Workshop
- 2004: Glasgow, UK - Chair, Nuclear RNA Splicing and Processing Minisymposium BioScience 2004
Professional Learned Society
- 2005 - present: Society for General Microbiology - Member. Elected to the Virus Group Committee 2005-2007
- 2004 - present: Tenovus - Committee Secretary. Co-Chair Symposium Committee, 2009-
- 2001 - present: American Society for Microbiology - Member
- 2000 - 2007: Royal Society of Chemistry - Committee Member, Nucleic Acids Group
- 2000 - 2007: International Papillomavirus Society - Member
- 1997 - present: RNA Society - Member
- 1995 - present: Biochemical Society - Chair, Genes Theme Panel 2003-2006; Vice Chair, Meetings Committee 2003-2006 ; Member of Council 2004-2006
- 1987 - 2007: Genetics Society - Member
- 1980 - 2007: British Society for Cell Biology - Member
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