Mr Leonard Ndwiga

  • Research Assistant (Virology)

Biography

In 2024, I joined the MRC-Centre for Virus Research - Genomics Unit as a Research Assistant, where I contribute to various genomic lab projects and bioinformatics. My previous role as a Research Officer, for KEMRI-Wellcome Trust, focused on the genomics of the Plasmodium falciparum parasite to understand antimalarial drug resistance in Kenya. I then advanced to the role of Head of Sequencing, overseeing projects on viruses, bacteria, and vector surveillance, and providing training to researchers. Throughout my career, I have gained proficiency in Oxford Nanopore and Illumina sequencing technologies, as well as bioinformatics analysis.

Research interests

My research interests are: Bioinformatics, viral genomics, host transcriptomics, Single-cell sequencing, training researchers in genomics, development and optimisation of sequencing protocols.

Publications

List by: Type | Date

Jump to: 2024 | 2023 | 2022 | 2021 | 2020 | 2019
Number of items: 15.

2024

Wamae, K. et al. (2024) Targeted amplicon deep sequencing of ama1 and mdr1 to track within-host P. falciparum diversity throughout treatment in a clinical drug trial. Wellcome Open Research, 9, 75. (doi: 10.12688/wellcomeopenres.17736.2) (PMID:37456906) (PMCID:PMC10349275)

2023

Okanda, D., Ndwiga, L. , Osoti, V., Achieng, N., Wambua, J., Ngetsa, C., Lubell-Doughtie, P., Shankar, A., Bejon, P. and Ochola-Oyier, L. I. (2023) Low frequency of Plasmodium falciparum hrp2/3 deletions from symptomatic infections at a primary healthcare facility in Kilifi, Kenya. Frontiers in Epidemiology, 3, 1083114. (doi: 10.3389/fepid.2023.1083114) (PMID:38455911) (PMCID:PMC10910971)

2022

Lucinde, R. K. et al. (2022) Sero-surveillance for IgG to SARS-CoV-2 at antenatal care clinics in three Kenyan referral hospitals: Repeated cross-sectional surveys 2020-21. PLoS ONE, 17(10), e0265478. (doi: 10.1371/journal.pone.0265478) (PMID:36240176) (PMCID:PMC9565697)

Wamae, K. et al. (2022) Amplicon sequencing as a potential surveillance tool for complexity of infection and drug resistance markers in plasmodium falciparum asymptomatic infections. Journal of Infectious Diseases, 226(1), 920–927-920–927. (doi: 10.1093/infdis/jiac144) (PMID:35429395) (PMCID:PMC7613600)

Kimenyi, K. M., Wamae, K., Ngoi, J. M., de Laurent, Z. R., Ndwiga, L. , Osoti, V., Obiero, G., Abdi, A. i., Bejon, P. and Ochola-Oyier, L. I. (2022) Maintenance of high temporal Plasmodium falciparum genetic diversity and complexity of infection in asymptomatic and symptomatic infections in Kilifi, Kenya from 2007 to 2018. Malaria Journal, 21, 192. (doi: 10.1186/s12936-022-04213-7) (PMID:35725456) (PMCID:PMC9207840)

Mwita Morobe, J. et al. (2022) Genomic epidemiology of SARS-CoV-2 in Seychelles, 2020-2021. Viruses, 14(6), 1318. (doi: 10.3390/v14061318) (PMID:35746789) (PMCID:PMC9231335)

Osoti, V. et al. (2022) Targeted amplicon deep sequencing for monitoring antimalarial resistance markers in Western Kenya. Antimicrobial Agents and Chemotherapy, 66(4), e0194521. (doi: 10.1128/aac.01945-21) (PMID:PMC9017353)

Said Mohammed, K. et al. (2022) An optimization of four SARS-CoV-2 qRT-PCR assays in a Kenyan laboratory to support the national COVID-19 rapid response teams. Wellcome Open Research, 5, 162. (doi: 10.12688/wellcomeopenres.16063.2) (PMID:35330938) (PMCID:PMC8921690)

Nyagwange, J. et al. (2022) Epidemiology of COVID-19 infections on routine polymerase chain reaction (PCR) and serology testing in Coastal Kenya. Wellcome Open Research, 7, 69. (doi: 10.12688/wellcomeopenres.17661.1) (PMID:35505772) (PMCID:PMC9034174)

Lambisia, A. W. et al. (2022) Optimization of the SARS-CoV-2 ARTIC Network V4 Primers and Whole Genome Sequencing Protocol. Frontiers in Medicine, 9, 836728. (doi: 10.3389/fmed.2022.836728) (PMID:35252269) (PMCID:PMC8891481)

Etyang, A. O. et al. (2022) Seroprevalence of antibodies to Severe Acute Respiratory Syndrome Coronavirus 2 among healthcare workers in Kenya. Clinical Infectious Diseases, 74(2), pp. 288-293. (doi: 10.1093/cid/ciab346) (PMID:33893491) (PMCID:PMC8135298)

2021

Ndwiga, L. et al. (2021) A review of the frequencies of Plasmodium falciparum Kelch 13 artemisinin resistance mutations in Africa. International Journal for Parasitology. Drugs and Drug Resistance, 16, pp. 155-161. (doi: 10.1016/j.ijpddr.2021.06.001) (PMID:34146993) (PMCID:PMC8219943)

Ndwiga, L. , Osoti, V., Ochwedo, K. O., Wamae, K., Bejon, P., Rayner, J. C., Githinji, G. and Ochola-Oyier, L. I. (2021) The Plasmodium falciparum Rh5 invasion protein complex reveals an excess of rare variant mutations. Malaria Journal, 20, 278. (doi: 10.1186/s12936-021-03815-x) (PMID:34162366) (PMCID:PMC8220363)

2020

Uyoga, S. et al. (2020) Seroprevalence of anti-SARS-CoV-2 IgG antibodies in Kenyan blood donors. Science, 371(6524), pp. 79-82. (doi: 10.1126/science.abe1916) (PMID:33177105) (PMCID:PMC7877494)

2019

Wamae, K., Okanda, D., Ndwiga, L. , Osoti, V., Kimeny, K. M., Abdi, A. I., Bejon, P., Sutherland, C. and Ochola-Oyier, L. I. (2019) No evidence of P. falciparum K13 artemisinin conferring mutations over a 24-year analysis in Coastal Kenya, but a near complete reversion to chloroquine wild type parasites. Antimicrobial Agents and Chemotherapy, 63(12), 01067-19. (doi: 10.1128/aac.01067-19) (PMID:31591113) (PMCID:PMC6879256)

This list was generated on Tue Nov 19 02:38:36 2024 GMT.
Jump to: Articles
Number of items: 15.

Articles

Wamae, K. et al. (2024) Targeted amplicon deep sequencing of ama1 and mdr1 to track within-host P. falciparum diversity throughout treatment in a clinical drug trial. Wellcome Open Research, 9, 75. (doi: 10.12688/wellcomeopenres.17736.2) (PMID:37456906) (PMCID:PMC10349275)

Okanda, D., Ndwiga, L. , Osoti, V., Achieng, N., Wambua, J., Ngetsa, C., Lubell-Doughtie, P., Shankar, A., Bejon, P. and Ochola-Oyier, L. I. (2023) Low frequency of Plasmodium falciparum hrp2/3 deletions from symptomatic infections at a primary healthcare facility in Kilifi, Kenya. Frontiers in Epidemiology, 3, 1083114. (doi: 10.3389/fepid.2023.1083114) (PMID:38455911) (PMCID:PMC10910971)

Lucinde, R. K. et al. (2022) Sero-surveillance for IgG to SARS-CoV-2 at antenatal care clinics in three Kenyan referral hospitals: Repeated cross-sectional surveys 2020-21. PLoS ONE, 17(10), e0265478. (doi: 10.1371/journal.pone.0265478) (PMID:36240176) (PMCID:PMC9565697)

Wamae, K. et al. (2022) Amplicon sequencing as a potential surveillance tool for complexity of infection and drug resistance markers in plasmodium falciparum asymptomatic infections. Journal of Infectious Diseases, 226(1), 920–927-920–927. (doi: 10.1093/infdis/jiac144) (PMID:35429395) (PMCID:PMC7613600)

Kimenyi, K. M., Wamae, K., Ngoi, J. M., de Laurent, Z. R., Ndwiga, L. , Osoti, V., Obiero, G., Abdi, A. i., Bejon, P. and Ochola-Oyier, L. I. (2022) Maintenance of high temporal Plasmodium falciparum genetic diversity and complexity of infection in asymptomatic and symptomatic infections in Kilifi, Kenya from 2007 to 2018. Malaria Journal, 21, 192. (doi: 10.1186/s12936-022-04213-7) (PMID:35725456) (PMCID:PMC9207840)

Mwita Morobe, J. et al. (2022) Genomic epidemiology of SARS-CoV-2 in Seychelles, 2020-2021. Viruses, 14(6), 1318. (doi: 10.3390/v14061318) (PMID:35746789) (PMCID:PMC9231335)

Osoti, V. et al. (2022) Targeted amplicon deep sequencing for monitoring antimalarial resistance markers in Western Kenya. Antimicrobial Agents and Chemotherapy, 66(4), e0194521. (doi: 10.1128/aac.01945-21) (PMID:PMC9017353)

Said Mohammed, K. et al. (2022) An optimization of four SARS-CoV-2 qRT-PCR assays in a Kenyan laboratory to support the national COVID-19 rapid response teams. Wellcome Open Research, 5, 162. (doi: 10.12688/wellcomeopenres.16063.2) (PMID:35330938) (PMCID:PMC8921690)

Nyagwange, J. et al. (2022) Epidemiology of COVID-19 infections on routine polymerase chain reaction (PCR) and serology testing in Coastal Kenya. Wellcome Open Research, 7, 69. (doi: 10.12688/wellcomeopenres.17661.1) (PMID:35505772) (PMCID:PMC9034174)

Lambisia, A. W. et al. (2022) Optimization of the SARS-CoV-2 ARTIC Network V4 Primers and Whole Genome Sequencing Protocol. Frontiers in Medicine, 9, 836728. (doi: 10.3389/fmed.2022.836728) (PMID:35252269) (PMCID:PMC8891481)

Etyang, A. O. et al. (2022) Seroprevalence of antibodies to Severe Acute Respiratory Syndrome Coronavirus 2 among healthcare workers in Kenya. Clinical Infectious Diseases, 74(2), pp. 288-293. (doi: 10.1093/cid/ciab346) (PMID:33893491) (PMCID:PMC8135298)

Ndwiga, L. et al. (2021) A review of the frequencies of Plasmodium falciparum Kelch 13 artemisinin resistance mutations in Africa. International Journal for Parasitology. Drugs and Drug Resistance, 16, pp. 155-161. (doi: 10.1016/j.ijpddr.2021.06.001) (PMID:34146993) (PMCID:PMC8219943)

Ndwiga, L. , Osoti, V., Ochwedo, K. O., Wamae, K., Bejon, P., Rayner, J. C., Githinji, G. and Ochola-Oyier, L. I. (2021) The Plasmodium falciparum Rh5 invasion protein complex reveals an excess of rare variant mutations. Malaria Journal, 20, 278. (doi: 10.1186/s12936-021-03815-x) (PMID:34162366) (PMCID:PMC8220363)

Uyoga, S. et al. (2020) Seroprevalence of anti-SARS-CoV-2 IgG antibodies in Kenyan blood donors. Science, 371(6524), pp. 79-82. (doi: 10.1126/science.abe1916) (PMID:33177105) (PMCID:PMC7877494)

Wamae, K., Okanda, D., Ndwiga, L. , Osoti, V., Kimeny, K. M., Abdi, A. I., Bejon, P., Sutherland, C. and Ochola-Oyier, L. I. (2019) No evidence of P. falciparum K13 artemisinin conferring mutations over a 24-year analysis in Coastal Kenya, but a near complete reversion to chloroquine wild type parasites. Antimicrobial Agents and Chemotherapy, 63(12), 01067-19. (doi: 10.1128/aac.01067-19) (PMID:31591113) (PMCID:PMC6879256)

This list was generated on Tue Nov 19 02:38:36 2024 GMT.

A head and shoulders shot of Leonard Ndwiga