Professor Nikolaj Gadegaard
- Professor (Biomedical Engineering)
telephone:
01413305243
email:
Nikolaj.Gadegaard@glasgow.ac.uk
School of Engineering, Rankine Building, Room 622, Glasgow G12 8LT
https://orcid.org/0000-0002-3396-846XResearch interests
Biography
I am an interdisciplinary researcher by nature with a focus on nanoscale engineering and biological systems, having more than 20 years of experience in the area. I graduated from Copenhagen University in 1998 with a BSc in chemistry and MSc in physics. After a brief position as a research assistant at Risø National Laboratory in Denmark, I continued my PhD studies in biophysics, graduating in 2002. This was followed by a brief post doc in the Centre for Cell Engineering at the University of Glasgow. In 2003 I received a personal fellowship from the Royal Society of Edinburgh and was appointed lecturer in 2006, senior lecturer in 2010, reader in 2012 and full professor in 2014. During 2013-2015 I was visiting professor at University of California Santa Barbara. In 2015 I was awarded an ERC Consolidator Award (FAKIR) to develop a mechanistic understanding of the interplay between engineered biomaterials and cell function/fate. Since 2018 I have had academic leadership as the Director of Research in the School of Engineering.
I was ellected Fellow of the Royal Society of Edinburgh in 2021.
I have published more than 150 papers in peer reviewed journals, many of significant impact (Nature and its sister journals, Advanced Materials, ACS Nano, etc.). Most notably is the work on the interplay between nanotopography and stem cells published in a series of papers in Nature Materials together with Prof. Matthew Dalby:
- The control of human mesenchymal cell differentiation using nanoscale symmetry and disorder
- Nanoscale surfaces for the long-term maintenance of mesenchymal stem cell phenotype and multipotency
- Harnessing nanotopography and integrin–matrix interactions to influence stem cell fate
Research directions
I lead a highly interdisciplinary research group of engineers, physicists, chemists, computer scientists and biologists. The group focuses on the following 4 connected activities:
Nanofabrication and manufacturing
In the past 20 years I have pioneered the use of nanoscale lithography and injection moulding to produce micro- and nanopatterned materials for biological applications. The majority of the research has focused on engineering new biomaterials for stem cell and regenerative medicine applications. More recently we have expanded the research, in collaboration with Prof Malcolm Kadodwala, to chiral plasmonic biosensors.
Group expertise: Large area nanopatterning and injection moulding of nanoscale materials. We are developing new tooling methods allowing us to design, manufacture and test new tools within one day. Uniquely, all resources are available in our lab
Mechanobiology
We are interestedin gaining a deeper understand of the nanoscale interplay between our materials and the biological response. This is dominated by interaction between the materials, through the focal adhesions in the cells and relayed to intracellular pathways. Here we have developed correlative super resolution and electron microscopy to visualise the molecular interactions of the cells with the engineered nanoscale materials.
Group expertise: Super resolution microscopy, correlated light and electron microscopy, FRET force sensors. These techniques are applied to cancer and stem cells.
Big data
We are collecting increasingly larger datasets, primarily though high-content microscopy. We have a number of high-content microscopes in our lab which we use to collect “traditional” fluorescent micrographs. At the same time, we are developing tools for high-content traction force microscopy enabling a new dimension of mechanobiology. In collaboration with Computing Science, we develop new pipelines for data analysis including machine learning and convoluted neural networks for deep learning.
Group expertise: High-content imaging og biomaterials, image analysis of large datasets, high-content mechanobiology analysis, advanced image and data analysis tools.
Organ-on-a-chip
The group is developing novel chips for the organ-on-a-chip community. We are developing novel approaches for chip design. This includes rapid prototyping as well as the potential to mass produce devices. In collaboration with partners we integrate organoids in the chip as well as developing enhanced differentiation protocols.
Group expertise: Rapid prototyping of microfluidic devices, integration of iPSC cells in the area of cardiac, liver, fat.
Publications
2024
Khandelwal, G. , John, D. A., Vivekananthan, V., Gadegaard, N. , Mulvihill, D. M. and Kim, S.-J. (2024) Growth of metal-organic framework ZIF-67 on cellulosic substrates for triboelectric nanogenerators. Nanoscale, (Accepted for Publication)
Young, P., Greer, A. I.M. , Smith, C.-A. , Silverwood, R., Tsimbouri, P. M. , Meek, D., Goodyear, C., Gadegaard, N. and Dalby, M. J. (2024) Titanium surface synergy: strontium incorporation and controlled disorder nanotopography optimise osteoinduction. ACS Applied Materials and Interfaces, (doi: 10.1021/acsami.4c04117) (Early Online Publication)
Menezes, P. D., Hunter, A., Dickson, T., Hecht, S., Kumar, C. , Busek, M., Krauss, S. and Gadegaard, N. (2024) Scalable, transparent and micro: 3D printed rapid tooling for injection moulded microfluidics. Advanced Engineering Materials, 26(20), 2400276. (doi: 10.1002/adem.202400276)
Kumar, R. et al. (2024) Electromagnetic enantiomer: chiral nanophotonic cavities for inducing chemical asymmetry. ACS Nano, 18(3), pp. 22220-22232. (doi: 10.1021/acsnano.4c05861) (PMID:39107108)
Lalaguna, P. L. et al. (2024) Spatial control of 2D nanomaterial electronic properties using chiral light beams. ACS Nano, (doi: 10.1021/acsnano.4c04506) (Early Online Publication)
Chaubey, S. K. et al. (2024) Ultrasensitive Raman detection of biomolecular conformation at the attomole scale using chiral nanophotonics. Small, (doi: 10.1002/smll.202404536) (Early Online Publication)
Aizenshtadt, A. et al. (2024) Pump-less, recirculating Organ-on-Chip (rOoC) platform to model the metabolic crosstalk between islets and liver. Advanced Healthcare Materials, 13(13), 2303785. (doi: 10.1002/adhm.202303785) (PMID:38221504)
Menezes, P.D., Hecht, S., Hunter, A. and Gadegaard, N. (2024) A membrane’s blueprint: in silico investigation of fluid flow and molecular transport as a function of membrane design parameters in organ-on-a-chip. Chemical Engineering Journal, 481, 148189. (doi: 10.1016/j.cej.2023.148189)
Schneider, J. , Basak, S., Hou, Y., Koo, J. H., Wardle, B. L., Gadegaard, N. and Kumar, S. (2024) 3D printed polyetheretherketone smart polymer nanocomposite scaffolds: mechanical, self-sensing, and biological attributes. Advanced Engineering Materials, 26(4), 2301659. (doi: 10.1002/adem.202301659)
Samuel, A. K., Faqeeh, A. H., Li, W., Ertekin, Z. , Wang, Y., Zhang, J., Gadegaard, N. , Moran, D. A.J. , Symes, M. D. and Ganin, A. Y. (2024) Assessing challenges of 2D-molybdenum ditelluride for efficient hydrogen generation in a full-scale proton exchange membrane (PEM) water electrolyzer. ACS Sustainable Chemistry and Engineering, 12(3), pp. 1276-1285. (doi: 10.1021/acssuschemeng.3c06616)
Koyroytsaltis-McQuire, D. J.P., Kumar, R., Javorfi, T., Siligardi, G., Gadegaard, N. and Kadodwala, M. (2024) Tuning dipolar and multipolar resonances of chiral silicon nanostructures for control of near field superchirality. Nanoscale, 16, pp. 110-122. (doi: 10.1039/d3nr05285k) (PMID:38063462)
2023
Peringath, A. R., Bayan, M. A.H., Beg, M., Jain, A., Pierini, F., Gadegaard, N. , Hogg, R. and Manjakkal, L. (2023) Chemical synthesis of polyaniline and polythiophene electrodes with excellent performance in supercapacitors. Journal of Energy Storage, 73(Part A), 108811. (doi: 10.1016/j.est.2023.108811)
Wallace, S. et al. (2023) Multiplexed biosensing of proteins and virions with disposable plasmonic assays. ACS Sensors, 8(9), pp. 3338-3348. (doi: 10.1021/acssensors.2c02238) (PMID:37610841) (PMCID:PMC10521139)
Kumar, C. , Laughtland, K., Bairagi, S., Gadegaard, N. and Mulvihill, D. (2023) Mechanics of Sliding Triboelectric Nanogenerators for Sustainable Energy Harvesting. 17th International Conference on Advances in Experimental Mechanics, Glasgow University Union, University of Glasgow, UK, 30 August 2023 - 01 September 2023.
Kartau, M. et al. (2023) Chiral metafilms and surface enhanced Raman scattering for enantiomeric discrimination of helicoid nanoparticles. Advanced Optical Materials, 11(9), 2202991. (doi: 10.1002/adom.202202991)
Guselnikova, O., Elashnikov, R., Svorcik, V., Kartau, M., Gilroy, C., Gadegaard, N. , Kadodwala, M. , Karimullah, A. S. and Lyutakov, O. (2023) Coupling of plasmonic hot spots with shurikens for superchiral SERS-based enantiomer recognition. Nanoscale Horizons, 8(4), pp. 499-508. (doi: 10.1039/D3NH00008G) (PMID:36752733)
Kumar, C. , Perris, J., Bairagi, S., Min, G., Xu, Y. , Gadegaard, N. and Mulvihill, D. M. (2023) Multiscale in-situ quantification of the role of surface roughness and contact area using a novel Mica-PVS triboelectric nanogenerator. Nano Energy, 107, 108122. (doi: 10.1016/j.nanoen.2022.108122)
Olender, J., Perris, J., Xu, Y. , Young, C. , Mulvihill, D. and Gadegaard, N. (2023) Gecko-inspired dry adhesives for heritage conservation – tackling the surface roughness with empirical testing and finite element modelling. Journal of Adhesion Science and Technology, 37(6), pp. 1091-1116. (doi: 10.1080/01694243.2022.2061153)
Ross, E. A. et al. (2023) Nanotopography reveals metabolites that maintain the immunomodulatory phenotype of mesenchymal stromal cells. Nature Communications, 14, 753. (doi: 10.1038/s41467-023-36293-7) (PMID:36765065) (PMCID:PMC9918539)
Bin Jaber, S., Xu, Y. , Kartal, M. E., Gadegaard, N. and Mulvihill, D. M. (2023) The static friction peak in reciprocating sliding. Tribology International, 180, 108240. (doi: 10.1016/j.triboint.2023.108240)
Hamilton, A., Xu, Y., Kartal, M. E., Kumar, S. , Gadegaard, N. and Mulvihill, D. M. (2023) Optimisation of interlocking microstructured adhesive joints via finite element modelling, design of experiments and 3D printing. International Journal of Adhesion and Adhesives, 120, 103292. (doi: 10.1016/j.ijadhadh.2022.103292)
Perris, J., Kumar, C. , Xu, Y. , Tassieri, M. , Kartal, M. E., Gadegaard, N. and Mulvihill, D. M. (2023) 3D printing and rapid replication of advanced numerically generated rough surface topographies in numerous polymers. Advanced Engineering Materials, 25(1), 2200832. (doi: 10.1002/adem.202200832)
2022
Koester, A. M., Geiser, A., Bowman, P. R.T., van de Linde, S., Gadegaard, N. , Bryant, N. J. and Gould, G. W. (2022) GLUT4 translocation and dispersal operate in multiple cell types and are negatively correlated with cell size in adipocytes. Scientific Reports, 12, 20535. (doi: 10.1038/s41598-022-24736-y) (PMID:36446811) (PMCID:PMC9708847)
Hawkes, W. et al. (2022) Regulation of cardiomyocyte adhesion and mechanosignalling through distinct nanoscale behaviour of integrin ligands mimicking healthy or fibrotic extracellular matrix. Philosophical Transactions of the Royal Society B: Biological Sciences, 377(1864), 20220021. (doi: 10.1098/rstb.2022.0021) (PMID:36189804)
Tabouillot, V. et al. (2022) Near-field probing of optical superchirality with plasmonic circularly polarized luminescence for enhanced bio-detection. ACS Photonics, 9(11), pp. 3617-3624. (doi: 10.1021/acsphotonics.2c01073) (PMID:36411820)
Koester, A. M. et al. (2022) EFR3 and phosphatidylinositol 4-kinase IIIα regulate insulin-stimulated glucose transport and GLUT4 dispersal in 3T3-L1 adipocytes. Bioscience Reports, 42(7), BSR20221181. (doi: 10.1042/BSR20221181) (PMID:35735144) (PMCID:PMC9272592)
Xu, Y., Scheibert, J., Gadegaard, N. and Mulvihill, D. M. (2022) An asperity-based statistical model for the adhesive friction of elastic nominally flat rough contact interfaces. Journal of the Mechanics and Physics of Solids, 164, 104878. (doi: 10.1016/j.jmps.2022.104878)
Kallas, P., Valen, H., Hulander, M., Gadegaard, N. , Stormonth-Darling, J. , O'Reilly, P., Thiede, B., Andersson, M. and Haugen, H. J. (2022) Protein-coated nanostructured surfaces affect the adhesion of Escherichia coli. Nanoscale, 14(20), pp. 7736-7746. (doi: 10.1039/D2NR00976E) (PMID:35579413) (PMCID:PMC9135173)
Huethorst, E. , Mortensen, P., Simitev, R. D. , Gao, H. , Pohjolainen, L., Talman, V., Ruskoaho, H., Burton, F. L., Gadegaard, N. and Smith, G. L. (2022) Conventional rigid 2D substrates cause complex contractile signals in monolayers of human induced pluripotent stem cell derived cardiomyocytes. Journal of Physiology, 600(3), pp. 483-507. (doi: 10.1113/JP282228) (PMID:34761809) (PMCID:PMC9299844)
Gilroy, C., Koyroytsaltis-McQuire, D. J. P., Gadegaard, N. , Karimullah, A. S. and Kadodwala, M. (2022) Superchiral hot-spots in “real” chiral plasmonic structures. Materials Advances, 3(1), pp. 346-354. (doi: 10.1039/D1MA00831E)
Koyroytsaltis-McQuire, D. J.P., Gilroy, C., Barron, L. D., Gadegaard, N. , Karimullah, A. S. and Kadodwala, M. (2022) Detecting antibody–antigen interactions with chiral plasmons: factors influencing chiral plasmonic sensing. Advanced Photonics Research, 3(1), 2100155. (doi: 10.1002/adpr.202100155)
2021
Hajji, M. et al. (2021) A chiral quantum metamaterial for hypersensitive biomolecule detection. ACS Nano, 15(12), pp. 19905-19916. (doi: 10.1021/acsnano.1c07408) (PMID:34846858)
Convery, N., Samardzhieva, I., Stormonth-Darling, J. M. , Harrison, S., Sullivan, G. J. and Gadegaard, N. (2021) 3D printed tooling for injection molded microfluidics. Macromolecular Materials and Engineering, 306(11), 2100464. (doi: 10.1002/mame.202100464)
Murphy, K. J. et al. (2021) Intravital imaging technology guides FAK-mediated priming in pancreatic cancer precision medicine according to Merlin status. Science Advances, 7(40), eabh0363. (doi: 10.1126/sciadv.abh0363) (PMID:34586840) (PMCID:PMC8480933)
Hamilton, A., Perris, J., Convery, N., Mulvihill, D. M. and Gadegaard, N. (2021) Flexible inserts for injection molding of complex micro-structured polymer components. Macromolecular Materials and Engineering, 306(9), 2100223. (doi: 10.1002/mame.202100223)
Perris, J., Xu, Y. , Kartal, M. E., Gadegaard, N. and Mulvihill, D. M. (2021) Tailorable and repeatable normal contact stiffness via micropatterned interfaces. Tribology Letters, 69, 106. (doi: 10.1007/s11249-021-01473-3)
Menezes, P. D., Gadegaard, N. , Natal Jorge, R. M. and Pinto, S. I. S. (2021) Modelling human liver microphysiology on a chip through a finite element based design approach. International Journal for Numerical Methods in Biomedical Engineering, 37(5), e3445. (doi: 10.1002/cnm.3445) (PMID:33522149)
Min, G., Xu, Y. , Cochran, P., Gadegaard, N. , Mulvihill, D. M. and Dahiya, R. (2021) Origin of the contact force-dependent response of triboelectric nanogenerators. Nano Energy, 83, 105829. (doi: 10.1016/j.nanoen.2021.105829)
Hamilton, A., Xu, Y. , Kartal, M. E., Gadegaard, N. and Mulvihill, D. M. (2021) Enhancing strength and toughness of adhesive joints via micro-structured mechanical interlocking. International Journal of Adhesion and Adhesives, 105, 102775. (doi: 10.1016/j.ijadhadh.2020.102775)
Mordue, J., O'Boyle, N., Gadegaard, N. and Roe, A. J. (2021) The force awakens: the dark side of mechanosensing in bacterial pathogens. Cellular Signalling, 78, 109867. (doi: 10.1016/j.cellsig.2020.109867) (PMID:33279672)
Bin Jaber, S., Hamilton, A., Xu, Y. , Kartal, M. E., Gadegaard, N. and Mulvihill, D. M. (2021) Friction of flat and micropatterned interfaces with nanoscale roughness. Tribology International, 153, 106563. (doi: 10.1016/j.triboint.2020.106563)
Gilroy, C., McKay, K., Devine, M., Webster, R. W.H., Gadegaard, N. , Karimullah, A. , Maclaren, D. and Kadodwala, M. (2021) Active chiral plasmonics: flexoelectric control of nanoscale chirality. Advanced Photonics Research, 2(1), 2000062. (doi: 10.1002/adpr.202000062)
2020
Kakkar, T. et al. (2020) Superchiral near fields detect virus structure. Light: Science and Applications, 9(1), 195. (doi: 10.1038/s41377-020-00433-1) (PMID:33298854) (PMCID:PMC7705013)
Greer, A. I.M. , Barbour, E., Cutiongco, M. F. , Stormonth-Darling, J. M. , Convery, N., Alsaigh, R. E., Lavery, M. P.J. and Gadegaard, N. (2020) Large volume nanoscale 3D printing: Nano-3DP. Applied Materials Today, 21, 100782. (doi: 10.1016/j.apmt.2020.100782)
Kallas, P., Haugen, H. J., Gadegaard, N. , Stormonth-Darling, J. , Hulander, M., Andersson, M. and Valen, H. (2020) Adhesion of Escherichia coli to nanostructured surfaces and the role of type 1 fimbriae. Nanomaterials, 10(11), 2247. (doi: 10.3390/nano10112247) (PMID:33198386) (PMCID:PMC7696039)
Kelly, C. et al. (2020) Controlling the symmetry of inorganic ionic nanofilms with optical chirality. Nature Communications, 11, 5169. (doi: 10.1038/s41467-020-18869-9) (PMID:33057000) (PMCID:PMC7560753)
Morgan, K. et al. (2020) Oxygen plasma treated substrates and specific nanopattern promote early differentiation and function of HepaRG progenitor cells. Tissue Engineering Part A, 26(19-20), pp. 1064-1076. (doi: 10.1089/ten.TEA.2019.0241) (PMID:32292123)
Xu, Y. , Min, G., Gadegaard, N. , Dahiya, R. and Mulvihill, D. M. (2020) A unified contact force-dependent model for triboelectric nanogenerators accounting for surface roughness. Nano Energy, 76, 105067. (doi: 10.1016/j.nanoen.2020.105067)
Ivanova, Y., Aitchison, I., Cannon, P. , Butler, D., Gadegaard, N. and Keith, N. (2020) Mapping of University Research Output and Impact to the United Nations Sustainable Development Goals. Project Report. OSF. (doi: 10.17605/OSF.IO/UFYTA).
Laux, L., Cutiongco, M. F.A. , Gadegaard, N. and Jensen, B. S. (2020) Interactive machine learning for fast and robust cell profiling. PLoS ONE, 15(9), e0237972. (doi: 10.1371/journal.pone.0237972) (PMID:32915784) (PMCID:PMC7485821)
Greer, A. et al. (2020) Nanopatterned titanium implants accelerate bone formation in vivo. ACS Applied Materials and Interfaces, 12(30), pp. 33541-33549. (doi: 10.1021/acsami.0c10273) (PMID:32633478) (PMCID:PMC7467557)
Greer, A. , Moodie, D., Kerr, G. and Gadegaard, N. (2020) Sol-gel coatings for subaquatic self-cleaning windows. Crystals, 10(5), 375. (doi: 10.3390/cryst10050375)
Cutiongco, M. F.A. , Jensen, B. S. , Reynolds, P. M. and Gadegaard, N. (2020) Predicting gene expression using morphological cell responses to nanotopography. Nature Communications, 11, 1384. (doi: 10.1038/s41467-020-15114-1) (PMID:32170111) (PMCID:PMC7070086)
Morris, S. et al. (2020) Characterisation of GLUT4 trafficking in HeLa cells: comparable kinetics and orthologous trafficking mechanisms to 3T3-L1 adipocytes. PeerJ, 8, e8751. (doi: 10.7717/peerj.8751) (PMID:32185116) (PMCID:PMC7060922)
Huethorst, E. , Cutiongco, M. F.A. , Campbell, F. A., Saeed, A. , Love, R., Reynolds, P. M. , Dalby, M. J. and Gadegaard, N. (2020) Customizable, engineered substrates for rapid screening of cellular cues. Biofabrication, 12(2), 025009. (doi: 10.1088/1758-5090/ab5d3f) (PMID:31783378) (PMCID:PMC7655147)
Rodier, M., Keijzer, C., Milner, J. , Karimullah, A. S. , Barron, L., Gadegaard, N. , Lapthorn, A. J. and Kadodwala, M. (2020) Biomacromolecular charge chirality detected using chiral plasmonic nanostructures. Nanoscale Horizons, 5, pp. 336-344. (doi: 10.1039/C9NH00525K)
2019
Juin, A. et al. (2019) N-WASP control of LPAR1 trafficking establishes response to self-generated LPA gradients to promote pancreatic cancer cell metastasis. Developmental Cell, 51(4), 431-445.e7. (doi: 10.1016/j.devcel.2019.09.018) (PMID:31668663) (PMCID:PMC6863394)
Convery, N. and Gadegaard, N. (2019) 30 years of microfluidics. Micro and Nano Engineering, 2, pp. 76-91. (doi: 10.1016/j.mne.2019.01.003)
Hawkes, W., Huang, D., Reynolds, P. , Hammond, L., Ward, M., Gadegaard, N. , Marshall, J. F., Iskratch, T. and Palma, M. (2019) Probing the nanoscale organisation and multivalency of cell surface receptors: DNA origami nanoarrays for cellular studies with single-molecule control. Faraday Discussions, 219, pp. 203-219. (doi: 10.1039/C9FD00023B) (PMID:31314021)
Rodier, M., Keijzer, C., Milner, J. , Karimullah, A. , Barron, L., Gadegaard, N. , Lapthorn, A. and Kadodwala, M. (2019) Probing specificity of protein-protein interactions with chiral plasmonic nanostructures. Journal of Physical Chemistry Letters, 10, pp. 6105-6111. (doi: 10.1021/acs.jpclett.9b02288) (PMID:31549842)
2018
Reynolds, P. M. , Holzmann Rasmussen, C., Hansson, M., Dufva, M., Riehle, M. O. and Gadegaard, N. (2018) Controlling fluid flow to improve cell seeding uniformity. PLoS ONE, 13(11), e0207211. (doi: 10.1371/journal.pone.0207211) (PMID:30440053) (PMCID:PMC6237340)
Bergholt, N. L., Foss, M., Saeed, A. , Gadegaard, N. , Lysdahl, H., Lind, M. and Foldager, C. B. (2018) Surface chemistry, substrate, and topography guide the behavior of human articular chondrocytes cultured in vitro. Journal of Biomedical Materials Research Part A, 106(11), pp. 2805-2816. (doi: 10.1002/jbm.a.36467) (PMID:29907992)
Francone, A., Kehoe, T., Obieta, I., Saez-Martinez, V., Bilbao, L., Khokhar, A., Gadegaard, N. , Simao, C., Kehagias, N. and Sotomayor Torres, C. (2018) Integrated 3D hydrogel waveguide out-coupler by step-and-repeat thermal nanoimprint lithography: a promising sensor device for water and pH. Sensors, 18(10), 3240. (doi: 10.3390/s18103240) (PMID:30261650) (PMCID:PMC6210174)
Newton, J., Ramage, G. , Gadegaard, N. , Zachs, W., Rogers, S. , Barrett, M. P. , Carruthers, G. and Burgess, K. (2018) Minimally-destructive atmospheric ionisation mass spectrometry authenticates authorship of historical manuscripts. Scientific Reports, 8, 10944. (doi: 10.1038/s41598-018-28810-2) (PMID:30050048) (PMCID:PMC6062563)
Kelly, C., Tullius, R., Lapthorn, A. J. , Gadegaard, N. , Cooke, G. , Barron, L. D., Karimullah, A. S. , Rotello, V. M. and Kadodwala, M. (2018) Chiral plasmonic fields probe structural order of biointerfaces. Journal of the American Chemical Society, 140(27), pp. 8509-8517. (doi: 10.1021/jacs.8b03634) (PMID:29909628)
Alakpa, E. V., Saeed, A. , Chung, P., Riehle, M. O. , Gadegaard, N. , Dalby, M. J. and Cusack, M. (2018) The prismatic topography of Pinctada maxima shell retains stem cell multipotency and plasticity in vitro. Advanced Biosystems, 2(6), 1800012. (doi: 10.1002/adbi.201800012)
Rath, N. et al. (2018) Rho kinase inhibition by AT13148 blocks pancreatic ductal adenocarinoma invasion and tumor growth. Cancer Research, 78(12), pp. 3321-3336. (doi: 10.1158/0008-5472.CAN-17-1339) (PMID:29669760) (PMCID:PMC6005347)
Alalwan, H., Nile, C. J. , Rajendran, R., McKerlie, R. , Reynolds, P. , Gadegaard, N. and Ramage, G. (2018) Nanoimprinting of biomedical polymers reduces candidal physical adhesion. Nanomedicine: Nanotechnology, Biology and Medicine, 14(3), pp. 1045-1049. (doi: 10.1016/j.nano.2018.01.011) (PMID:29408656)
Kelly, C., Khorashad, L. K., Gadegaard, N. , Barron, L. D., Govorov, A. O., Karimullah, A. S. and Kadodwala, M. (2018) Controlling metamaterial transparency with superchiral fields. ACS Photonics, 5(2), pp. 535-543. (doi: 10.1021/acsphotonics.7b01071)
Allan, C., Ker, A., Smith, C.-A. , Tsimbouri, P. , Borsoi, J., O'Neill, S., Gadegaard, N. , Dalby, M. J. and Meek, D. (2018) Osteoblast response to disordered nanotopography. Journal of Tissue Engineering, 9, pp. 1-7. (doi: 10.1177/2041731418784098) (PMID:30034770) (PMCID:PMC6048666)
2017
Tullius, R. et al. (2017) Superchiral plasmonic phase sensitivity for fingerprinting of protein interface structure. ACS Nano, 11(12), pp. 12049-12056. (doi: 10.1021/acsnano.7b04698) (PMID:29220155)
Alakpa, E. V., Burgess, K. E.V., Chung, P., Riehle, M. O. , Gadegaard, N. , Dalby, M. J. and Cusack, M. (2017) Nacre topography produces higher crystallinity in bone than chemically induced osteogenesis. ACS Nano, 11(7), pp. 6717-6727. (doi: 10.1021/acsnano.7b01044) (PMID:28665112)
Sharma, S., Saeed, A. , Johnson, C., Gadegaard, N. and Cass, A. E.G. (2017) Rapid, low cost prototyping of transdermal devices for personal healthcare monitoring. Sensing and Bio-Sensing Research, 13, pp. 104-108. (doi: 10.1016/j.sbsr.2016.10.004) (PMID:28424755) (PMCID:PMC5384990)
Lee, L. et al. (2017) Nanotopography controls cell cycle changes involved with skeletal stem cell self-renewal and multipotency. Biomaterials, 116, pp. 10-20. (doi: 10.1016/j.biomaterials.2016.11.032) (PMID:27914982) (PMCID:PMC5226065)
Donnelly, H. , Smith, C.-A. , Sweeten, P. E., Gadegaard, N. , Meek, D., D'Este, M., Mata, A., Eglin, D. and Dalby, M. J. (2017) Bone and cartilage differentiation of a single stem cell population driven by material interface. Journal of Tissue Engineering, 8, pp. 1-10. (doi: 10.1177/2041731417705615) (PMID:28567273) (PMCID:PMC5438107)
Messerschmidt, M., Greer, A. , Schlachter, F., Barnett, J., Thesen, M. W., Gadegaard, N. , Grutzner, G. and Schleunitz, A. (2017) New organic photo-curable nanoimprint resist ≪mr-NIL210≫ for high volume fabrication applying soft PDMS-based stamps. Journal of Photopolymer Science and Technology, 30(5), pp. 605-611. (doi: 10.2494/photopolymer.30.605)
Rostam, H. M., Reynolds, P. M. , Alexander, M. R., Gadegaard, N. and Ghaemmaghami, A. M. (2017) Image based machine learning for identification of macrophage subsets. Scientific Reports, 7, 3521. (doi: 10.1038/s41598-017-03780-z) (PMID:28615717) (PMCID:PMC5471192)
2016
Huethorst, E., Hortigon, M. , Zamora-Rodriguez, V., Reynolds, P.M. , Burton, F., Smith, G. and Gadegaard, N. (2016) Enhanced human-induced pluripotent stem cell derived cardiomyocyte maturation using a dual microgradient substrate. ACS Biomaterials Science and Engineering, 2(12), pp. 2231-2239. (doi: 10.1021/acsbiomaterials.6b00426) (PMID:27990488) (PMCID:PMC5155309)
Greer, A. I.M. , Della-Rosa, B., Khokhar, A. Z. and Gadegaard, N. (2016) Step-and-repeat nanoimprint-, photo- and laser-lithography from one customised CNC machine. Nanoscale Research Letters, 11, 129. (doi: 10.1186/s11671-016-1341-9) (PMID:26956598) (PMCID:PMC4783314)
Young, P.S., Greer, A.I.M. , Tsimbouri, P.M. , Meek, R.M.D., Gadegaard, N. and Dalby, M.J. (2016) Precision-Engineered Nanotopographical Surfaces to Control Osteoclast Differentiation. In: The British Orthopaedic Research Society (BORS) Annual Conference, Glasgow, UK, 5-6 Sept 2016,
Connell, A., Reynolds, P. M. , Saeed, A. and Gadegaard, N. (2016) Fabrication of mesoscale topographical gradients in bulk titanium and their use in injection moulding. Microelectronic Engineering, 164, pp. 36-42. (doi: 10.1016/j.mee.2016.07.004)
Colquhoun, R., Gadegaard, N. , Healy, D. M. and Tanner, K. E. (2016) Characterisation of CorGlaes® Pure 107 fibres for biomedical applications. Journal of Materials Science: Materials in Medicine, 27, 149. (doi: 10.1007/s10856-016-5752-z)
Jack, C., Karimullah, A. S. , Leyman, R., Tullius, R., Rotello, V. M., Cooke, G. , Gadegaard, N. , Barron, L. D. and Kadodwala, M. (2016) Biomacromolecular stereostructure mediates mode hybridization in chiral plasmonic nanostructures. Nano Letters, 16(9), pp. 5806-5814. (doi: 10.1021/acs.nanolett.6b02549) (PMID:27547978)
Aguayo, S., Strange, A., Gadegaard, N. , Dalby, M.J. and Bozec, L. (2016) Influence of biomaterial nanotopography on the adhesive and elastic properties of Staphylococcus aureus cells. RSC Advances, 6(92), pp. 89347-89355. (doi: 10.1039/C6RA12504B)
Stormonth-Darling, J. M. , Saeed, A. , Reynolds, P. M. and Gadegaard, N. (2016) Injection molding micro- and nanostructures in thermoplastic elastomers. Macromolecular Materials and Engineering, 301(8), pp. 964-971. (doi: 10.1002/mame.201600011) (PMID:31217727) (PMCID:PMC6563430)
Ngandu Mpoyi, E., Cantini, M. , Reynolds, P. M. , Gadegaard, N. , Dalby, M. J. and Salmerón-Sánchez, M. (2016) Protein adsorption as a key mediator in the nanotopographical control of cell behavior. ACS Nano, 10(7), pp. 6638-6647. (doi: 10.1021/acsnano.6b01649) (PMID:27391047) (PMCID:PMC4980054)
Greer, A. I.M. , Vasiev, I., Della-Rosa, B. and Gadegaard, N. (2016) Fluorinated ethylene-propylene: a complementary alternative to PDMS for nanoimprint stamps. Nanotechnology, 27(15), 155301. (doi: 10.1088/0957-4484/27/15/155301) (PMID:26938810)
Jack, C. et al. (2016) Spatial control of chemical processes on nanostructures through nano-localized water heating. Nature Communications, 7, 10946. (doi: 10.1038/ncomms10946) (PMID:26961708) (PMCID:PMC4792951)
Greer, A. I. M. , Lim, T. S., Brydone, A. S. and Gadegaard, N. (2016) Mechanical compatibility of sol–gel annealing with titanium for orthopaedic prostheses. Journal of Materials Science: Materials in Medicine, 27, 21. (doi: 10.1007/s10856-015-5611-3) (PMID:26691162) (PMCID:PMC4686541)
2015
Holzmann Rasmussen, C., Reynolds, P. , Petersen, D. R., Hansson, M., McMeeking, R. M., Dufva, M. and Gadegaard, N. (2015) Enhanced differentiation of human embryonic stem cells towards definitive endoderm on ultrahigh aspect ratio nanopillars. Advanced Functional Materials, 26(6), pp. 815-823. (doi: 10.1002/adfm.201504204)
Karimullah, A. S. , Jack, C., Tullius, R., Rotello, V. M., Cooke, G. , Gadegaard, N. , Barron, L. D. and Kadodwala, M. (2015) Disposable plasmonics: plastic templated plasmonic metamaterials with tunable chirality. Advanced Materials, 27(37), pp. 5610-5616. (doi: 10.1002/adma.201501816)
Tullius, R., Karimullah, A. S. , Rodier, M., Fitzpatrick, B., Gadegaard, N. , Barron, L. D., Rotello, V. M., Cooke, G. , Lapthorn, A. and Kadodwala, M. (2015) "Superchiral” spectroscopy: detection of protein higher order hierarchical structure with chiral plasmonic nanostructures. Journal of the American Chemical Society, 137(26), pp. 8380-8383. (doi: 10.1021/jacs.5b04806) (PMID:26102606)
Gupta, S., Hannah, S., Watson, C.P., Šutta, P., Pedersen, R.H., Gadegaard, N. and Gleskova, H. (2015) Ozone oxidation methods for aluminum oxide formation: Application to low-voltage organic transistors. Organic Electronics, 21, pp. 132-137. (doi: 10.1016/j.orgel.2015.03.007)
Chong, D.S.T., Turner, L.-A., Gadegaard, N. , Seifalian, A.M., Dalby, M.J. and Hamilton, G. (2015) Nanotopography and plasma treatment: redesigning the surface for vascular graft endothelialisation. European Journal of Vascular and Endovascular Surgery, 49(3), pp. 335-343. (doi: 10.1016/j.ejvs.2014.12.008) (PMID:25579872)
Pedersen, R. H., Xu, Q., Stormonth-Darling, J. M. and Gadegaard, N. (2015) Strategies for high quality injection moulding of polymer nanopillars. Macromolecular Materials and Engineering, 300(2), pp. 172-180. (doi: 10.1002/mame.201400134)
Greer, A. I. M. and Gadegaard, N. (2015) A novel, organic, UV-sensitive resist ideal for nanoimprint-, photo- and laser lithography in an air atmosphere. Electronic Materials Letters, 11(4), pp. 544-551. (doi: 10.1007/s13391-015-4401-x)
Pemberton, G. D., Childs, P., Reid, S., Nikukar, H., Tsimbouri, M. , Gadegaard, N. , Curtis, A. S. and Dalby, M. J. (2015) Nanoscale stimulation of osteoblastogenesis from mesenchymal stem cells: nanotopography and nanokicking. Nanomedicine, 10(4), pp. 547-560. (doi: 10.2217/nnm.14.134) (PMID:25723089)
Reynolds, P. M. and Gadegaard, N. (2015) Polymer gradient surfaces for biomedical applications. In: Rodríguez-Hernández,, J. and Cortajarena, A. L. (eds.) Design of Polymeric Platforms for Selective Biorecognition. Springer International Publishing, pp. 93-122. ISBN 9783319170602 (doi: 10.1007/978-3-319-17061-9_5)
Young, P.S., Tsimbouri, P.M. , Gadegaard, N. , Meek, R.M.D. and Dalby, M.J. (2015) Osteoclastogenesis/osteoblastogenesis using human bone marrow-derived cocultures on nanotopographical polymer surfaces. Nanomedicine, 10(6), pp. 949-957. (doi: 10.2217/nnm.14.146) (PMID:25867859)
2014
Wang, J. R., Ahmed, S. F. , Gadegaard, N. , Meek, R.M. D., Dalby, M. J. and Yarwood, S. J. (2014) Nanotopology potentiates growth hormone signalling and osteogenesis of mesenchymal stem cells. Growth Hormone and IGF Research, 24(6), pp. 245-250. (doi: 10.1016/j.ghir.2014.10.003) (PMID:25466909)
Busche, C. et al. (2014) Design and fabrication of memory devices based on nanoscale polyoxometalate clusters. Nature, 515(7528), pp. 545-549. (doi: 10.1038/nature13951) (PMID:25409147)
Yang, J., McNamara, L. E., Gadegaard, N. , Alakpa, E. V., Burgess, K. V., Meek, D. and Dalby, M. J. (2014) Nanotopographical induction of osteogenesis through adhesion, bone morphogenic protein co-signaling and regulation of microRNAs. ACS Nano, 8(10), pp. 9941-9953. (doi: 10.1021/nn504767g) (PMID:25227207)
Palacios-Cuesta, M., Vasiev, I., Gadegaard, N. , Rodríguez-Hernández, J. and García, O. (2014) Direct micrometer patterning and functionalization of polymer blend surfaces by using hot embossing. European Polymer Journal, 59, pp. 333-340. (doi: 10.1016/j.eurpolymj.2014.07.020)
Donoghue, P. S., Sun, T., Gadegaard, N. , Riehle, M. O. and Barnett, S. C. (2014) Development of a novel 3D culture system for screening features of a complex implantable device for CNS repair. Molecular Pharmaceutics, 11(7), pp. 2143-2150. (doi: 10.1021/mp400526n) (PMID:24279373) (PMCID:PMC4087043)
Grenci, G., Sovernigo, E., Khokhar, A. Z., Gadegaard, N. , Prasciolu, M. and Tormen, M. (2014) Microfabrication of sharp blazed gratings by a two-step height amplification process based on soft and deep X-ray lithography. Sensors and Actuators A: Physical, 205, pp. 111-118. (doi: 10.1016/j.sna.2013.09.036)
Dalby, M. J. , Gadegaard, N. and Oreffo, R. O.C. (2014) Harnessing nanotopography and integrin–matrix interactions to influence stem cell fate. Nature Materials, 13(6), pp. 558-569. (doi: 10.1038/NMAT3980)
Stormonth-Darling, J. M. , Pedersen, R. H., How, C. and Gadegaard, N. (2014) Injection moulding of ultra high aspect ratio nanostructures using coated polymer tooling. Journal of Micromechanics and Microengineering, 24(7), 075019. (doi: 10.1088/0960-1317/24/7/075019)
2013
Kadodwala, M. , Abdulrahman, N., Syme, C. D. , Jack, C., Barron, L. D., Karimullah, A. and Gadegaard, N. (2013) The origin of off–resonance non-linear optical activity of a gold chiral nanomaterial. Nanoscale, 5(24), pp. 12651-12657. (doi: 10.1039/c3nr04746f)
Greer, A. I.M. , Seunarine, K., Khokhar, A. Z., MacLaren, I. , Brydone, A. S., Moran, D. A.J. and Gadegaard, N. (2013) Increased efficiency of direct nanoimprinting on planar and curved bulk titanium through surface modification. Microelectronic Engineering, 112, pp. 67-73. (doi: 10.1016/j.mee.2013.05.016)
Hansen, A.M., Sewell, A.L., Pedersen, R.H., Long, D.-L. , Gadegaard, N. and Marquez, R. (2013) Tunable BODIPY derivatives amenable to "click" and peptide chemistry. Tetrahedron, 69(39), pp. 8527-8533. (doi: 10.1016/j.tet.2013.05.037)
Vasiev, I., Greer, A.I.M. , Khokhar, A.Z., Stormonth-Darling, J. , Tanner, K.E. and Gadegaard, N. (2013) Self-folding nano- and micropatterned hydrogel tissue engineering scaffolds by single step photolithographic process. Microelectronic Engineering, 108, pp. 76-81. (doi: 10.1016/j.mee.2013.04.003)
Kingham, E., White, K., Gadegaard, N. , Dalby, M.J. and Oreffo, R.O.C. (2013) Nanotopographical cues augment mesenchymal differentiation of human embryonic stem cells. Small, 9(12), pp. 2140-2151. (doi: 10.1002/smll.201202340)
Tsimbouri, P. M. , Murawski, K., Hamilton, G., Herzyk, P. , Oreffo, R. O.C., Gadegaard, N. and Dalby, M. J. (2013) A genomics approach in determining nanotopographical effects on MSC phenotype. Biomaterials, 34(9), pp. 2177-2184. (doi: 10.1016/j.biomaterials.2012.12.019) (PMID:23312853) (PMCID:PMC3573234)
Reynolds, P.M. , Pedersen, R.H., Stormonth-Darling, J., Dalby, M.J. , Riehle, M.O. and Gadegaard, N. (2013) Label-free segmentation of co-cultured cells on a nanotopographical gradient. Nano Letters, 13(2), pp. 570-576. (doi: 10.1021/nl304097p)
Donoghue, P.S., Lamond, R., Boomkamp, S.D., Tao, S., Gadegaard, N. , Riehle, M.O. and Barnett, S.C. (2013) The development of a ε-polycaprolactone (PCL) scaffold for CNS repair. Tissue Engineering Part A, 19(3), (doi: 10.1089/ten.TEA.2012.0382) (PMID:22994455)
Karimullah, A. S. , Cumming, D. R.S. , Riehle, M. and Gadegaard, N. (2013) Development of a conducting polymer cell impedance sensor. Sensors and Actuators B: Chemical, 176, pp. 667-674. (doi: 10.1016/j.snb.2012.09.075)
Roach, P., Parker, T., Gadegaard, N. and Alexander, M. R. (2013) A bio-inspired neural environment to control neurons comprising radial glia, substrate chemistry and topography. Biomaterials Science, 1, pp. 83-93. (doi: 10.1039/C2BM00060A)
Sun, T., Donoghue, P.S., Higginson, J.R., Gadegaard, N. , Barnett, S.C. and Riehle, M.O. (2013) A miniaturized bioreactor system for the evaluation of cell interaction with designed substrates in perfusion culture. Journal of Tissue Engineering and Regenerative Medicine, 6(S3), S4-S14. (doi: 10.1002/term.510) (PMID:22170765)
2012
Tsimbouri, P.M. , McMurray, R.J., Burgess, K.V., Alakpa, E.V., Reynolds, P. , Murawski, K., Kingham, E., Oreffo, R.O.C., Gadegaard, N. and Dalby, M.J. (2012) Using nanotopography and metabolomics to identify biochemical effectors of multipotency. ACS Nano, 6(11), pp. 10239-10249. (doi: 10.1021/nn304046m)
Stormonth-Darling, J.M. and Gadegaard, N. (2012) Injection moulding difficult nanopatterns with hybrid polymer inlays. Macromolecular Materials and Engineering, 297(11), pp. 1075-1080. (doi: 10.1002/mame.201100397)
Pedersen, R.H., Kustanovich, K. and Gadegaard, N. (2012) Single-step 3D nanolithography using plasma polymerized hexane films. Microelectronic Engineering, 98, pp. 167-170. (doi: 10.1016/j.mee.2012.07.054)
Reynolds, P.M. , Pedersen, R.H., Riehle, M.O. and Gadegaard, N. (2012) A dual gradient assay for the parametric analysis of cell-surface interactions. Small, 8(16), pp. 2541-2547. (doi: 10.1002/smll.201200235)
Pedersen, R.H., Scurr, D.J., Roach, P., Alexander, M.R. and Gadegaard, N. (2012) Full-thickness characterization of plasma polymerized hexane films irradiated by an electron beam. Plasma Processes and Polymers, 9(1), pp. 22-27. (doi: 10.1002/ppap.201100067)
Abdulrahman, N.A., Fan, Z., Tonooka, T., Kelly, S.M. , Gadegaard, N. , Hendry, E., Govorov, A.O. and Kadodwala, M. (2012) Induced chirality through electromagnetic coupling between chiral molecular layers and plasmonic nanostructures. Nano Letters, 12(2), pp. 977-983. (doi: 10.1021/nl204055r)
Greer, A. I. M. , Seunarine, K., Khokhar, A. Z., Li, X. , Moran, D. A. J. and Gadegaard, N. (2012) Direct Nano-Patterning of Commercially Pure Titanium with Ultra-Nanocrystalline Diamond Stamps. In: SBDD XVII Diamond Workshop, Hasselt, Belgium, March 2012,
Greer, A.I.M. , Seunarine, K., Khokhar, A., Li, X. , Moran, D. and Gadegaard, N. (2012) Direct nanopatterning of commercially pure titanium with ultra-nanocrystalline diamond stamps. Physica Status Solidi A: Applications and Materials Science, 209(9), pp. 1721-1725. (doi: 10.1002/pssa.201200057)
Reboud, V. et al. (2012) Enhanced light extraction in ITO-free OLEDs using double-sided printed electrodes. Nanoscale, 4(11), p. 3495. (doi: 10.1039/c2nr12068b)
2011
Kirchhof, K., Andar, A., Yin, H.B. , Gadegaard, N. , Riehle, M.O. and Groth, T. (2011) Polyelectrolyte multilayers generated in a microfluidic device with pH gradients direct adhesion and movement of cells. Lab on a Chip, 11(19), pp. 3326-3335. (doi: 10.1039/C1LC20408D)
McMurray, R.J., Gadegaard, N. , Tsimbouri, P.M. , Burgess, K.V., McNamara, L.E., Tare, R., Murawski, K., Kingham, E., Oreffo, R.O.C. and Dalby, M.J. (2011) Nanoscale surfaces for the long-term maintenance of mesenchymal stem cell phenotype and multipotency. Nature Materials, 10(8), pp. 637-644. (doi: 10.1038/nmat3058) (PMID:21765399)
Kantawong, F., Robertson, M.E., Gadegaard, N. , Oreffo, R.O.C., Burchmore, R.J. and Dalby, M.J. (2011) Protein expression of STRO-1 cells in response to different topographic features. Journal of Tissue Engineering, 2011, 534603. (doi: 10.4061/2011/534603) (PMID:21772957) (PMCID:PMC3136090)
Kingham, E.J., Tsimbouri, P.M. , Gadegaard, N. , Dalby, M.J. and Oreffo, R.O.C. (2011) Nanotopography induced osteogenic differentiation of human stem cells. Bone, 48(Sup. 2), S108-S109. (doi: 10.1016/j.bone.2011.03.177)
Sun, T., Donoghue, P.S., Higginson, J.R., Gadegaard, N. , Barnett, S.C. and Riehle, M.O. (2011) The interactions of astrocytes and fibroblasts with defined pore structures in static and perfusion cultures. Biomaterials, 32(8), pp. 2021-2031. (doi: 10.1016/j.biomaterials.2010.11.046) (PMID:21163522) (PMCID:PMC3440599)
Khokhar, A.Z., Gaston, A., Obieta, I. and Gadegaard, N. (2011) Compact LED based nanoimprinter for UV-NIL. Microelectronic Engineering, 88(11), pp. 3347-3352. (doi: 10.1016/j.mee.2011.06.023)
Khokhar, A.Z., Tsimbouri, P.M. , Dalby, M.J. and Gadegaard, N. (2011) Development of ultra-hard stamps for titanium patterning. In: 37th International Conference on Micro and Nano Engineering: MNE 2011, Berlin, 19 - 23 September 2011,
Obieta, I., Bilbao, L., Corres, A., Gadegaard, N. , Khokhar, A.Z. and Gadegaard, N. (2011) 3D nanopatterning of photosensitive hydrogels. In: NanoSpain Conference 2011, Bilbao, Spain, 11-14 April 2011,
Tsimbouri, P. , Gadegaard, N. , Burgess, K., Kingham, E., Oreffo, R.O.C. and Dalby, M.J. (2011) Mechanotransduction and skeletal stem cell differentiation in response to bioactive nanotopography. Histology and Histopathology: Cellular and Molecular Biology, 26(S1), 31.012.
Tsimbouri, P. , Gadegaard, N. , Oreffo, R.O.C. and Dalby, M.J. (2011) Effects of nanotopography on cell adhesion, morphology and differentiation. European Cells and Materials, 22(S2), p. 14.
2010
Csaderova, L., Martines, E., Seunarine, K., Gadegaard, N. , Wilkinson, C.D.W. and Riehle, M.O. (2010) A biodegradable and biocompatible regular nanopattern for large-scale selective cell growth. Small, 6(23), pp. 2755-2761. (doi: 10.1002/smll.201000193)
Hendry, E., Carpy, T., Johnston, J., Popland, M., Mikhaylovskiy, R.V., Lapthorn, A. , Kelly, S.M., Barron, L.D., Gadegaard, N. and Kadodwala, M. (2010) Ultrasensitive detection and characterization of biomolecules using superchiral fields. Nature Nanotechnology, 5(11), pp. 783-787. (doi: 10.1038/nnano.2010.209)
Altomare, L., Gadegaard, N. , Visai, L., Tanzi, M.C. and Fare, S. (2010) Biodegradable microgrooved polymeric surfaces obtained by photolithography for skeletal muscle cell orientation and myotube development. Acta Biomaterialia, 6(6), pp. 1948-1957. (doi: 10.1016/j.actbio.2009.12.040)
Altomare, L., Riehle, M.O. , Gadegaard, N. , Tanzi, M.C. and Farè, S. (2010) Microcontact printing of fibronectin on a biodegradable polymeric surface for skeletal muscle cell orientation. International Journal of Artificial Organs, 33(8), pp. 535-543.
Gaston, A., Khokhar, A.Z., Bilbao, L., Saez-Martinez, V., Corres, A., Obieta, I. and Gadegaard, N. (2010) Nanopatterned UV curable hydrogels for biomedical applications. Microelectronic Engineering, 87(5-8), pp. 1057-1061. (doi: 10.1016/j.mee.2009.11.089)
Ji, B., Cusack, M. , Freer, A., Dobson, P.S. , Gadegaard, N. and Yin, H. (2010) Control of crystal polymorph in microfluidics using molluscan 28 kDa Ca2+-binding protein. Integrative Biology, 2, pp. 528-535. (doi: 10.1039/c0ib00007h) (PMID:20820629)
Majani, R., Zelzer, M., Gadegaard, N. , Rose, F.R. and Alexander, M.R. (2010) Preparation of Caco-2 cell sheets using plasma polymerised acrylic acid as a weak boundary layer. Biomaterials, 31(26), pp. 6764-6771. (doi: 10.1016/j.biomaterials.2010.05.049)
McMurray, R.J., Gadegaard, N. , Dalby, M.J. , Tsimbouri, P.M. , Maclaine, S., Meek, D., McNamara, L.E., Child, H. and Berry, C.C. (2010) Research highlights. Nanomedicine, 5(10), pp. 1495-1499. (doi: 10.2217/nnm.10.119)
Pedersen, R.H., Hamzah, M., Thoms, S. , Roach, P., Alexander, M.R. and Gadegaard, N. (2010) Electron beam lithography using plasma polymerized hexane as resist. Microelectronic Engineering, 87(5-8), pp. 1112-1114. (doi: 10.1016/j.mee.2009.11.043)
Roach, P., Parker, T., Gadegaard, N. and Alexander, M.R. (2010) Surface strategies for control of neuronal cell adhesion: A review. Surface Science Reports, 65(6), pp. 145-173. (doi: 10.1016/j.surfrep.2010.07.001)
2009
Kantawong, F., Burchmore, R.J.S. , Gadegaard, N. , Oreffo, R.O.C. and Dalby, M.J. (2009) Proteomic analysis of human osteoprogenitor response to disordered nanotopography. Journal of the Royal Society: Interface, (doi: 10.1098/rsif.2008.0447)
Biggs, M.J.P., Richards, R.G., Gadegaard, N. , McMurray, R.J., Affrosman, S., Wilkinson, C.D., Oreffo, R.O.C. and Dalby, M.J. (2009) Interactions with nanoscale topography: adhesion quantification and signal transduction in cells of osteogenic and multipotent lineage. Journal of Biomedical Materials Research Part A, 91A(1), pp. 195-208. (doi: 10.1002/jbm.a.32196)
Biggs, M.J.P., Richards, R.G., Gadegaard, N. , Wilkinson, C.D.W., Oreffo, R.O.C. and Dalby, M.J. (2009) The use of nanoscale topography to modulate the dynamics of adhesion formation in primary osteoblasts and ERK/MAPK signalling in STRO-1+ enriched skeletal stem cells. Biomaterials, 30(28), pp. 5094-5103. (doi: 10.1016/j.biomaterials.2009.05.049)
Malm, J., Giannaras, D., Riehle, M.O. , Gadegaard, N. and Sjovall, P. (2009) Fixation and drying protocols for the preparation of cell samples for time-of-flight secondary ion mass spectrometry analysis. Analytical Chemistry, 81(17), pp. 7107-7205. (doi: 10.1021/ac900636v)
Kantawong, F., Burgess, K.E.V., Jayawardena, K., Hart, A., Burchmore, R.J. , Gadegaard, N. , Oreffo, R.O.C. and Dalby, M.J. (2009) Whole proteome analysis of osteoprogenitor differentiation induced by disordered nanotopography and mediated by ERK signalling. Biomaterials, 30(27), pp. 4723-4731. (doi: 10.1016/j.biomaterials.2009.05.040)
Seunarine, K., Curtis, A.S.G., Meredith, D.O., Wilkinson, C.D.W., Riehle, M.O. and Gadegaard, N. (2009) A hierarchical response of cells to perpendicular micro- and nanometric textural cues. IEEE Transactions on NanoBioscience, 8(3), pp. 219-225. (doi: 10.1109/TNB.2009.2016477)
Streb, C., Tsunashima, R., MacLaren, D.A. , McGlone, T., Akutagawa, T., Nakamura, T., Scandurra, A., Pignataro, B., Gadegaard, N. and Cronin, L. (2009) Supramolecular silver polyoxometalate architectures direct the growth of composite semiconducting nanostructures. Angewandte Chemie (International Edition), 48(35), pp. 6490-6493. (doi: 10.1002/anie.200901650)
Rossi, L., Riehle, M.O. , McNamara, L.E., Burchmore, R. , Dalby, M.J. , McMurray, R.J., Gadegaard, N. , Ahmed, S. and Tsimbouri, P.M. (2009) Research highlights. Nanomedicine, 4(6), pp. 613-617. (doi: 10.2217/nnm.09.46)
Glidle, A., Hillman, A.R., Rydeo, K.S., Smith, E.L., Cooper, J.M. , Gadegaard, N. , Webster, J.R.P., Dalgliesh, R. and Cubitt, R. (2009) Use of neutron reflectivity to measure the dynamics of solvation and structural changes in polyvinylferrocene films during electrochemically controlled redox cycling. Langmuir, 25(7), pp. 4093-4103. (doi: 10.1021/la803234e)
Krasa, D., Wilkinson, C.D.W., Gadegaard, N. , Kong, X., Zhou, H., Roberts, A.P., Muxworthy, A.R. and Williams, W. (2009) Nanofabrication of two-dimensional arrays of magnetite particles for fundamental rock magnetic studies. Journal of Geophysical Research: Solid Earth, 114, B02104. (doi: 10.1029/2008JB006017)
Lamond, R., Donoghue, P., Gadegaard, N. , Riehle, M. and Barnett, S. (2009) Developing a biodegradable construct to enhance axonal alignment following a spinal cord injury. Glia, 57(13), S162-S162.
MacLaren, D.A. , Johnston, J., Duncan, D.A., Marchetto, H., Dhesi, S.S., Gadegaard, N. and Kadodwala, M. (2009) Asymmetric photoelectron transmission through chirally-sculpted, polycrystalline gold. Physical Chemistry Chemical Physics, 11(38), pp. 8413-8416. (doi: 10.1039/b911447e)
Song, Y.F., McMillan, N., Long, D.L. , Kane, S., Malm, J., Riehle, M. , Pradeep, C.P., Gadegaard, N. and Cronin, L. (2009) Micropatterned Surfaces with Covalently Grafted Unsymmetrical Polyoxometalate-Hybrid Clusters Lead to Selective Cell Adhesion. Journal of the American Chemical Society, 131(4), pp. 1340-1341. (doi: 10.1021/ja807091v)
Yang, J., Rose, F.R.A.J., Gadegaard, N. and Alexander, M. (2009) A high-throughput assay of cell-surface interactions using topographical and chemical gradients. Advanced Materials, 21(3), pp. 300-304. (doi: 10.1002/adma.200801942)
Yang, J., Rose, F.R.A.J., Gadegaard, N. and Alexander, M.R. (2009) Effect of sessile drop volume on the wetting anisotropy observed on grooved surfaces. Langmuir, 25(5), pp. 2567-2571. (doi: 10.1021/la803942h)
Yin, H. , Bozhi, J., Dobson, P.S. , Mosbahi, K., Glidle, A., Gadegaard, N. , Freer, A., Cooper, J.M. and Cusack, M. (2009) Screening of biomineralization using microfluidics. Analytical Chemistry, 81(1), pp. 473-478. (doi: 10.1021/ac801980b) (PMID:19117468)
Zelzer, M., Scurr, D., Abdullah, B., Urquhart, A.J., Gadegaard, N. , Bradley, J.W. and Alexander, M.R. (2009) Influence of the plasma sheath on plasma polymer deposition in advance of a mask and down pores. Journal of Physical Chemistry B, 113(25), pp. 8487-8494. (doi: 10.1021/jp902137y)
2008
Dalby, M.J. , McNamara, L.E., Riehle, M.O. , Burchmore, R.J.S. , Kuntanawat, P., McMurray, R.J., Gadegaard, N. and Andar, A. (2008) Research highlights. Nanomedicine, 3(4), pp. 411-414. (doi: 10.2217/17435889.3.4.411)
Fleming, C., Long, D. L. , McMillan, N., Johnston, J., Bovet, N., Dhanak, V., Gadegaard, N. , Kogerler, P., Cronin, L. and Kadodwala, M. (2008) Reversible electron-transfer reactions within a nanoscale metal oxide cage mediated by metallic substrates. Nature Nanotechnology, 3(4), pp. 229-233. (doi: 10.1038/nnano.2008.66)
Song, Y.-F., McMillan, N., Long, D.-L. , Thiel, J., Ding, Y., Chen, H., Gadegaard, N. and Cronin, L. (2008) Design of hydrophobic polyoxometalate hybrid assemblies beyond surfactant encapsulation. Chemistry: A European Journal, 14(8), pp. 2349-2354. (doi: 10.1002/chem.200701629)
Dalby, M.J. , Gadegaard, N. and Wilkinson, C.D.W. (2008) The response of fibroblasts to hexagonal nanotopography fabricated by electron beam lithography. Journal of Biomedical Materials Research Part A, 84A(4), pp. 973-979. (doi: 10.1002/jbm.a.31409)
Gadegaard, N. , Chen, X.Y., Rutten, F.J.M. and Alexander, M.R. (2008) High-energy electron beam lithography of octadecylphosphonic acid monolayers on aluminum. Langmuir, 24(5), pp. 2057-2063. (doi: 10.1021/la701733n)
Gadegaard, N. , Dalby, M.J. , Riehle, M.O. and Wilkinson, C.D.W. (2008) Optimizing substrate disorder for bone tissue engineering of mesenchymal stem cells. Journal of Vacuum Science and Technology B: Microelectronics and Nanometer Structures, 26(6), pp. 2554-2557. (doi: 10.1116/1.2978407)
Gadegaard, N. , Seunarine, K., Smith, D.J.A., Meredith, D., Wilkinson, C.D.W. and Riehle, M.O. (2008) A hybrid three-dimensional nanofabrication method for producing vascular tissue engineering scaffold. Japanese Journal of Applied Physics, 47(9), pp. 7415-7419. (doi: 10.1143/JAPP.47.7415)
Seunarine, K., Meredith, D.O., Riehle, M.O. , Wilkinson, C.D.W. and Gadegaard, N. (2008) Biodegradable polymer tubes with litho graphically controlled 3D micro- and nanotopography. Microelectronic Engineering, 85(5-6), pp. 1350-1354. (doi: 10.1016/j.mee.2008.02.002)
2007
Bovet, N., McMillan, N., Gadegaard, N. and Kadodwala, M. (2007) Supramolecular assembly facilitating adsorbate-induced chiral electronic states in a metal surface. Journal of the American Chemical Society, 111(33), pp. 10005-10011. (doi: 10.1021/jp074056s)
Biggs, M.J.P., Richards, R.G., Gadegaard, N. , Wilkinson, C.D.W. and Dalby, M.J. (2007) The effects of nanoscale pits on primary human osteoblast adhesion formation and cellular spreading. Journal of Materials Science: Materials in Medicine, 18, pp. 399-404. (doi: 10.1007/s10856-006-0705-6)
Biggs, M.J.P., Richards, R.G., Gadegaard, N. , Wilkinson, C.D.W. and Dalby, M.J. (2007) Regulation of implant surface cell adhesion: Characterization and quantification of S-phase primary osteoblast adhesions on biomimetic nanoscale substrates. Journal of Orthopaedic Research, 25, pp. 273-282. (doi: 10.1002/jor.20319)
Dalby, M.J. , Biggs, M.J.P., Gadegaard, N. , Kalna, G., Wilkinson, C.D.W. and Curtis, A.S.G. (2007) Nanotopographical stimulation of mechanotransduction and changes in interphase centromere positioning. Journal of Cellular Biochemistry, 100, pp. 326-338. (doi: 10.1002/jcb.21058)
Dalby, M.J. , Gadegaard, N. , Herzyk, P. , Agheli, H., Sutherland, D.S. and Wilkinson, C.D.W. (2007) Group analysis of regulation of fibroblast genome on low-adhesion nanostructures. Biomaterials, 28, pp. 1761-1769. (doi: 10.1016/j.biomaterials.2006.11.049)
Dalby, M.J. , Gadegaard, N. , Tare, R., Andar, A., Riehle, M.O. , Herzyk, P. , Wilkinson, C.D.W. and Oreffo, R.O.C. (2007) The control of human mesenchymal cell differentiation using nanoscale symmetry and disorder. Nature Materials, 6(12), pp. 997-1003. (doi: 10.1038/nmat2013)
McMillan, N., Smith, L.V., de la Fuente, J.M., Parenty, A.D.C., Gadegaard, N. , Pitt, A.R., Thomson, K., MacKenzie, C., Kelly, S. and Cronin, L. (2007) Incorporation of N-heterocyclic cations into proteins with a highly directed chemical modification. Chemical Communications, 25, pp. 2581-2583. (doi: 10.1039/b702575k)
Song, Y.-F., Abbas, H., Ritchie, C., McMillian, N., Long, D.-L. , Gadegaard, N. and Cronin, L. (2007) From polyoxometalate building blocks to polymers and materials: the silver connection. Journal of Materials Chemistry, 17, pp. 1903-1908. (doi: 10.1039/b617830h)
2006
Curtis, A.S.G., Dalby, M.J. and Gadegaard, N. (2006) Cell signaling arising from nanotopography: implications for nanomedical devices. Nanomedicine, 1, pp. 67-72. (doi: 10.2217/17435889.1.1.67)
Gadegaard, N. (2006) Atomic force microscopy in biology: technology and techniques. Biotechnic and Histochemistry, 81, pp. 87-97. (doi: 10.1080/10520290600783143)
Pranov, H., Rasmussen, H., Larsen, N. and Gadegaard, N. (2006) On the injection molding of nanostructured polymer surfaces. Polymer Engineering and Science, 46, pp. 160-171. (doi: 10.1002/pen.20459)
Seunarine, K., Gadegaard, N. , Tormen, M., O Meredith, D., O Riehle, M. and Wilkinson, C. (2006) 3D polymer scaffolds for tissue engineering. Nanomedicine, 1, pp. 281-296. (doi: 10.2217/17435889.1.3.281)
Seunarine, K., Tormen, M., Gadegaard, N. , Riehle, M. , Wilkinson, C.D.W., Businaro, L. and Romanato, F. (2006) Progress towards tubes with regular nanopatterned inner surfaces. Journal of Vacuum Science and Technology B: Microelectronics and Nanometer Structures, 24(6), pp. 3258-3262. (doi: 10.1116/1.2357970)
2005
Glidle, A., Hadyoon, C.S., Gadegaard, N. , Cooper, J.M. , Hillman, A.R., Wilson, A.R., Ryder, K.S., Webster, J.R.P. and Cubitt, R. (2005) Evaluating the influence of deposition conditions on solvation of reactive conducting polymers with neutron reflectivity. Journal of Physical Chemistry B, 109(30), pp. 14335-14343. (doi: 10.1021/jp0515030)
2004
Gadegaard, N. , Dalby, M.J. , Riehle, M.O. , Curtis, A.S.G. and Affrossman, S. (2004) Tubes with controllable internal nanotopography. Advanced Materials, 20, pp. 1857-1860. (doi: 10.1002/adma.200400408)
Dalby, M.J. , Gadegaard, N. , Riehle, M.O. , Wilkinson, C.D.W. and Curtis, A.S.G. (2004) Investigating filopodia sensing using arrays of defined nano-pits down to 35 nm diameter in size. International Journal of Biochemistry and Cell Biology, 36(10), pp. 2005-2015. (doi: 10.1016/j.biocel.2004.03.001)
Dalby, M.J. , Giannaras, D., Riehle, M.O. , Gadegaard, N. , Affrossman, S. and Curtis, A.S.G. (2004) Rapid fibroblast adhesion to 27 nm high polymer demixed nano-topography. Biomaterials, 25(1), pp. 77-83. (doi: 10.1016/S0142-9612(03)00475-7)
Cooper, J.M. , Cubitt, R., Dalgliesh, R.M., Gadegaard, N. , Glidle, A., Hillman, A.R., Mortimer, R.J., Ryder, K.S. and Smith, E.L. (2004) Dynamic in situ electrochemical neutron reflectivity measurements. Journal of the American Chemical Society, 126(47), pp. 15362-15363. (doi: 10.1021/ja044682s)
Curtis, A.S.G., Gadegaard, N. , Dalby, M.J. , Riehle, M.O. , Wilkinson, C.D.W. and Aitchison, G. (2004) Cells react to nanoscale order and symmetry in their surroundings. IEEE Transactions on NanoBioscience, 3(1), pp. 61-65. (doi: 10.1109/TNB.2004.824276)
2003
Gadegaard, N. , Thoms, S., Macintyre, D., Mcghee, K., Gallagher, J., Casey, B. and Wilkinson, C. (2003) Arrays of nano-dots for cellular engineering. Microelectronic Engineering, 67-8, pp. 162-168. (doi: 10.1016/S0167-9317(03)00067-4)
Wilkinson, C.D.W., Thoms, S. , Macintyre, D., Curtis, A.S.G., Riehle, M. and Gadegaard, N. (2003) Nanofabrication of structures for cell engineering. Proceedings of the SPIE: The International Society for Optical Engineering, 5220, pp. 1-9. (doi: 10.1117/12.509749)
2002
Hillman, A.R., Bailey, L., Glidle, A., Cooper, J.M. , Gadegaard, N. and Webster, J.R.P. (2002) Spatial distributions of polymer and mobile species in poly(o-toluidine) films. Journal of Electroanalytical Chemistry, 532, pp. 269-276.
Articles
Khandelwal, G. , John, D. A., Vivekananthan, V., Gadegaard, N. , Mulvihill, D. M. and Kim, S.-J. (2024) Growth of metal-organic framework ZIF-67 on cellulosic substrates for triboelectric nanogenerators. Nanoscale, (Accepted for Publication)
Young, P., Greer, A. I.M. , Smith, C.-A. , Silverwood, R., Tsimbouri, P. M. , Meek, D., Goodyear, C., Gadegaard, N. and Dalby, M. J. (2024) Titanium surface synergy: strontium incorporation and controlled disorder nanotopography optimise osteoinduction. ACS Applied Materials and Interfaces, (doi: 10.1021/acsami.4c04117) (Early Online Publication)
Menezes, P. D., Hunter, A., Dickson, T., Hecht, S., Kumar, C. , Busek, M., Krauss, S. and Gadegaard, N. (2024) Scalable, transparent and micro: 3D printed rapid tooling for injection moulded microfluidics. Advanced Engineering Materials, 26(20), 2400276. (doi: 10.1002/adem.202400276)
Kumar, R. et al. (2024) Electromagnetic enantiomer: chiral nanophotonic cavities for inducing chemical asymmetry. ACS Nano, 18(3), pp. 22220-22232. (doi: 10.1021/acsnano.4c05861) (PMID:39107108)
Lalaguna, P. L. et al. (2024) Spatial control of 2D nanomaterial electronic properties using chiral light beams. ACS Nano, (doi: 10.1021/acsnano.4c04506) (Early Online Publication)
Chaubey, S. K. et al. (2024) Ultrasensitive Raman detection of biomolecular conformation at the attomole scale using chiral nanophotonics. Small, (doi: 10.1002/smll.202404536) (Early Online Publication)
Aizenshtadt, A. et al. (2024) Pump-less, recirculating Organ-on-Chip (rOoC) platform to model the metabolic crosstalk between islets and liver. Advanced Healthcare Materials, 13(13), 2303785. (doi: 10.1002/adhm.202303785) (PMID:38221504)
Menezes, P.D., Hecht, S., Hunter, A. and Gadegaard, N. (2024) A membrane’s blueprint: in silico investigation of fluid flow and molecular transport as a function of membrane design parameters in organ-on-a-chip. Chemical Engineering Journal, 481, 148189. (doi: 10.1016/j.cej.2023.148189)
Schneider, J. , Basak, S., Hou, Y., Koo, J. H., Wardle, B. L., Gadegaard, N. and Kumar, S. (2024) 3D printed polyetheretherketone smart polymer nanocomposite scaffolds: mechanical, self-sensing, and biological attributes. Advanced Engineering Materials, 26(4), 2301659. (doi: 10.1002/adem.202301659)
Samuel, A. K., Faqeeh, A. H., Li, W., Ertekin, Z. , Wang, Y., Zhang, J., Gadegaard, N. , Moran, D. A.J. , Symes, M. D. and Ganin, A. Y. (2024) Assessing challenges of 2D-molybdenum ditelluride for efficient hydrogen generation in a full-scale proton exchange membrane (PEM) water electrolyzer. ACS Sustainable Chemistry and Engineering, 12(3), pp. 1276-1285. (doi: 10.1021/acssuschemeng.3c06616)
Koyroytsaltis-McQuire, D. J.P., Kumar, R., Javorfi, T., Siligardi, G., Gadegaard, N. and Kadodwala, M. (2024) Tuning dipolar and multipolar resonances of chiral silicon nanostructures for control of near field superchirality. Nanoscale, 16, pp. 110-122. (doi: 10.1039/d3nr05285k) (PMID:38063462)
Peringath, A. R., Bayan, M. A.H., Beg, M., Jain, A., Pierini, F., Gadegaard, N. , Hogg, R. and Manjakkal, L. (2023) Chemical synthesis of polyaniline and polythiophene electrodes with excellent performance in supercapacitors. Journal of Energy Storage, 73(Part A), 108811. (doi: 10.1016/j.est.2023.108811)
Wallace, S. et al. (2023) Multiplexed biosensing of proteins and virions with disposable plasmonic assays. ACS Sensors, 8(9), pp. 3338-3348. (doi: 10.1021/acssensors.2c02238) (PMID:37610841) (PMCID:PMC10521139)
Kartau, M. et al. (2023) Chiral metafilms and surface enhanced Raman scattering for enantiomeric discrimination of helicoid nanoparticles. Advanced Optical Materials, 11(9), 2202991. (doi: 10.1002/adom.202202991)
Guselnikova, O., Elashnikov, R., Svorcik, V., Kartau, M., Gilroy, C., Gadegaard, N. , Kadodwala, M. , Karimullah, A. S. and Lyutakov, O. (2023) Coupling of plasmonic hot spots with shurikens for superchiral SERS-based enantiomer recognition. Nanoscale Horizons, 8(4), pp. 499-508. (doi: 10.1039/D3NH00008G) (PMID:36752733)
Kumar, C. , Perris, J., Bairagi, S., Min, G., Xu, Y. , Gadegaard, N. and Mulvihill, D. M. (2023) Multiscale in-situ quantification of the role of surface roughness and contact area using a novel Mica-PVS triboelectric nanogenerator. Nano Energy, 107, 108122. (doi: 10.1016/j.nanoen.2022.108122)
Olender, J., Perris, J., Xu, Y. , Young, C. , Mulvihill, D. and Gadegaard, N. (2023) Gecko-inspired dry adhesives for heritage conservation – tackling the surface roughness with empirical testing and finite element modelling. Journal of Adhesion Science and Technology, 37(6), pp. 1091-1116. (doi: 10.1080/01694243.2022.2061153)
Ross, E. A. et al. (2023) Nanotopography reveals metabolites that maintain the immunomodulatory phenotype of mesenchymal stromal cells. Nature Communications, 14, 753. (doi: 10.1038/s41467-023-36293-7) (PMID:36765065) (PMCID:PMC9918539)
Bin Jaber, S., Xu, Y. , Kartal, M. E., Gadegaard, N. and Mulvihill, D. M. (2023) The static friction peak in reciprocating sliding. Tribology International, 180, 108240. (doi: 10.1016/j.triboint.2023.108240)
Hamilton, A., Xu, Y., Kartal, M. E., Kumar, S. , Gadegaard, N. and Mulvihill, D. M. (2023) Optimisation of interlocking microstructured adhesive joints via finite element modelling, design of experiments and 3D printing. International Journal of Adhesion and Adhesives, 120, 103292. (doi: 10.1016/j.ijadhadh.2022.103292)
Perris, J., Kumar, C. , Xu, Y. , Tassieri, M. , Kartal, M. E., Gadegaard, N. and Mulvihill, D. M. (2023) 3D printing and rapid replication of advanced numerically generated rough surface topographies in numerous polymers. Advanced Engineering Materials, 25(1), 2200832. (doi: 10.1002/adem.202200832)
Koester, A. M., Geiser, A., Bowman, P. R.T., van de Linde, S., Gadegaard, N. , Bryant, N. J. and Gould, G. W. (2022) GLUT4 translocation and dispersal operate in multiple cell types and are negatively correlated with cell size in adipocytes. Scientific Reports, 12, 20535. (doi: 10.1038/s41598-022-24736-y) (PMID:36446811) (PMCID:PMC9708847)
Hawkes, W. et al. (2022) Regulation of cardiomyocyte adhesion and mechanosignalling through distinct nanoscale behaviour of integrin ligands mimicking healthy or fibrotic extracellular matrix. Philosophical Transactions of the Royal Society B: Biological Sciences, 377(1864), 20220021. (doi: 10.1098/rstb.2022.0021) (PMID:36189804)
Tabouillot, V. et al. (2022) Near-field probing of optical superchirality with plasmonic circularly polarized luminescence for enhanced bio-detection. ACS Photonics, 9(11), pp. 3617-3624. (doi: 10.1021/acsphotonics.2c01073) (PMID:36411820)
Koester, A. M. et al. (2022) EFR3 and phosphatidylinositol 4-kinase IIIα regulate insulin-stimulated glucose transport and GLUT4 dispersal in 3T3-L1 adipocytes. Bioscience Reports, 42(7), BSR20221181. (doi: 10.1042/BSR20221181) (PMID:35735144) (PMCID:PMC9272592)
Xu, Y., Scheibert, J., Gadegaard, N. and Mulvihill, D. M. (2022) An asperity-based statistical model for the adhesive friction of elastic nominally flat rough contact interfaces. Journal of the Mechanics and Physics of Solids, 164, 104878. (doi: 10.1016/j.jmps.2022.104878)
Kallas, P., Valen, H., Hulander, M., Gadegaard, N. , Stormonth-Darling, J. , O'Reilly, P., Thiede, B., Andersson, M. and Haugen, H. J. (2022) Protein-coated nanostructured surfaces affect the adhesion of Escherichia coli. Nanoscale, 14(20), pp. 7736-7746. (doi: 10.1039/D2NR00976E) (PMID:35579413) (PMCID:PMC9135173)
Huethorst, E. , Mortensen, P., Simitev, R. D. , Gao, H. , Pohjolainen, L., Talman, V., Ruskoaho, H., Burton, F. L., Gadegaard, N. and Smith, G. L. (2022) Conventional rigid 2D substrates cause complex contractile signals in monolayers of human induced pluripotent stem cell derived cardiomyocytes. Journal of Physiology, 600(3), pp. 483-507. (doi: 10.1113/JP282228) (PMID:34761809) (PMCID:PMC9299844)
Gilroy, C., Koyroytsaltis-McQuire, D. J. P., Gadegaard, N. , Karimullah, A. S. and Kadodwala, M. (2022) Superchiral hot-spots in “real” chiral plasmonic structures. Materials Advances, 3(1), pp. 346-354. (doi: 10.1039/D1MA00831E)
Koyroytsaltis-McQuire, D. J.P., Gilroy, C., Barron, L. D., Gadegaard, N. , Karimullah, A. S. and Kadodwala, M. (2022) Detecting antibody–antigen interactions with chiral plasmons: factors influencing chiral plasmonic sensing. Advanced Photonics Research, 3(1), 2100155. (doi: 10.1002/adpr.202100155)
Hajji, M. et al. (2021) A chiral quantum metamaterial for hypersensitive biomolecule detection. ACS Nano, 15(12), pp. 19905-19916. (doi: 10.1021/acsnano.1c07408) (PMID:34846858)
Convery, N., Samardzhieva, I., Stormonth-Darling, J. M. , Harrison, S., Sullivan, G. J. and Gadegaard, N. (2021) 3D printed tooling for injection molded microfluidics. Macromolecular Materials and Engineering, 306(11), 2100464. (doi: 10.1002/mame.202100464)
Murphy, K. J. et al. (2021) Intravital imaging technology guides FAK-mediated priming in pancreatic cancer precision medicine according to Merlin status. Science Advances, 7(40), eabh0363. (doi: 10.1126/sciadv.abh0363) (PMID:34586840) (PMCID:PMC8480933)
Hamilton, A., Perris, J., Convery, N., Mulvihill, D. M. and Gadegaard, N. (2021) Flexible inserts for injection molding of complex micro-structured polymer components. Macromolecular Materials and Engineering, 306(9), 2100223. (doi: 10.1002/mame.202100223)
Perris, J., Xu, Y. , Kartal, M. E., Gadegaard, N. and Mulvihill, D. M. (2021) Tailorable and repeatable normal contact stiffness via micropatterned interfaces. Tribology Letters, 69, 106. (doi: 10.1007/s11249-021-01473-3)
Menezes, P. D., Gadegaard, N. , Natal Jorge, R. M. and Pinto, S. I. S. (2021) Modelling human liver microphysiology on a chip through a finite element based design approach. International Journal for Numerical Methods in Biomedical Engineering, 37(5), e3445. (doi: 10.1002/cnm.3445) (PMID:33522149)
Min, G., Xu, Y. , Cochran, P., Gadegaard, N. , Mulvihill, D. M. and Dahiya, R. (2021) Origin of the contact force-dependent response of triboelectric nanogenerators. Nano Energy, 83, 105829. (doi: 10.1016/j.nanoen.2021.105829)
Hamilton, A., Xu, Y. , Kartal, M. E., Gadegaard, N. and Mulvihill, D. M. (2021) Enhancing strength and toughness of adhesive joints via micro-structured mechanical interlocking. International Journal of Adhesion and Adhesives, 105, 102775. (doi: 10.1016/j.ijadhadh.2020.102775)
Mordue, J., O'Boyle, N., Gadegaard, N. and Roe, A. J. (2021) The force awakens: the dark side of mechanosensing in bacterial pathogens. Cellular Signalling, 78, 109867. (doi: 10.1016/j.cellsig.2020.109867) (PMID:33279672)
Bin Jaber, S., Hamilton, A., Xu, Y. , Kartal, M. E., Gadegaard, N. and Mulvihill, D. M. (2021) Friction of flat and micropatterned interfaces with nanoscale roughness. Tribology International, 153, 106563. (doi: 10.1016/j.triboint.2020.106563)
Gilroy, C., McKay, K., Devine, M., Webster, R. W.H., Gadegaard, N. , Karimullah, A. , Maclaren, D. and Kadodwala, M. (2021) Active chiral plasmonics: flexoelectric control of nanoscale chirality. Advanced Photonics Research, 2(1), 2000062. (doi: 10.1002/adpr.202000062)
Kakkar, T. et al. (2020) Superchiral near fields detect virus structure. Light: Science and Applications, 9(1), 195. (doi: 10.1038/s41377-020-00433-1) (PMID:33298854) (PMCID:PMC7705013)
Greer, A. I.M. , Barbour, E., Cutiongco, M. F. , Stormonth-Darling, J. M. , Convery, N., Alsaigh, R. E., Lavery, M. P.J. and Gadegaard, N. (2020) Large volume nanoscale 3D printing: Nano-3DP. Applied Materials Today, 21, 100782. (doi: 10.1016/j.apmt.2020.100782)
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Convery, N. and Gadegaard, N. (2019) 30 years of microfluidics. Micro and Nano Engineering, 2, pp. 76-91. (doi: 10.1016/j.mne.2019.01.003)
Hawkes, W., Huang, D., Reynolds, P. , Hammond, L., Ward, M., Gadegaard, N. , Marshall, J. F., Iskratch, T. and Palma, M. (2019) Probing the nanoscale organisation and multivalency of cell surface receptors: DNA origami nanoarrays for cellular studies with single-molecule control. Faraday Discussions, 219, pp. 203-219. (doi: 10.1039/C9FD00023B) (PMID:31314021)
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Reynolds, P. M. , Holzmann Rasmussen, C., Hansson, M., Dufva, M., Riehle, M. O. and Gadegaard, N. (2018) Controlling fluid flow to improve cell seeding uniformity. PLoS ONE, 13(11), e0207211. (doi: 10.1371/journal.pone.0207211) (PMID:30440053) (PMCID:PMC6237340)
Bergholt, N. L., Foss, M., Saeed, A. , Gadegaard, N. , Lysdahl, H., Lind, M. and Foldager, C. B. (2018) Surface chemistry, substrate, and topography guide the behavior of human articular chondrocytes cultured in vitro. Journal of Biomedical Materials Research Part A, 106(11), pp. 2805-2816. (doi: 10.1002/jbm.a.36467) (PMID:29907992)
Francone, A., Kehoe, T., Obieta, I., Saez-Martinez, V., Bilbao, L., Khokhar, A., Gadegaard, N. , Simao, C., Kehagias, N. and Sotomayor Torres, C. (2018) Integrated 3D hydrogel waveguide out-coupler by step-and-repeat thermal nanoimprint lithography: a promising sensor device for water and pH. Sensors, 18(10), 3240. (doi: 10.3390/s18103240) (PMID:30261650) (PMCID:PMC6210174)
Newton, J., Ramage, G. , Gadegaard, N. , Zachs, W., Rogers, S. , Barrett, M. P. , Carruthers, G. and Burgess, K. (2018) Minimally-destructive atmospheric ionisation mass spectrometry authenticates authorship of historical manuscripts. Scientific Reports, 8, 10944. (doi: 10.1038/s41598-018-28810-2) (PMID:30050048) (PMCID:PMC6062563)
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Alakpa, E. V., Saeed, A. , Chung, P., Riehle, M. O. , Gadegaard, N. , Dalby, M. J. and Cusack, M. (2018) The prismatic topography of Pinctada maxima shell retains stem cell multipotency and plasticity in vitro. Advanced Biosystems, 2(6), 1800012. (doi: 10.1002/adbi.201800012)
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Alalwan, H., Nile, C. J. , Rajendran, R., McKerlie, R. , Reynolds, P. , Gadegaard, N. and Ramage, G. (2018) Nanoimprinting of biomedical polymers reduces candidal physical adhesion. Nanomedicine: Nanotechnology, Biology and Medicine, 14(3), pp. 1045-1049. (doi: 10.1016/j.nano.2018.01.011) (PMID:29408656)
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Allan, C., Ker, A., Smith, C.-A. , Tsimbouri, P. , Borsoi, J., O'Neill, S., Gadegaard, N. , Dalby, M. J. and Meek, D. (2018) Osteoblast response to disordered nanotopography. Journal of Tissue Engineering, 9, pp. 1-7. (doi: 10.1177/2041731418784098) (PMID:30034770) (PMCID:PMC6048666)
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Alakpa, E. V., Burgess, K. E.V., Chung, P., Riehle, M. O. , Gadegaard, N. , Dalby, M. J. and Cusack, M. (2017) Nacre topography produces higher crystallinity in bone than chemically induced osteogenesis. ACS Nano, 11(7), pp. 6717-6727. (doi: 10.1021/acsnano.7b01044) (PMID:28665112)
Sharma, S., Saeed, A. , Johnson, C., Gadegaard, N. and Cass, A. E.G. (2017) Rapid, low cost prototyping of transdermal devices for personal healthcare monitoring. Sensing and Bio-Sensing Research, 13, pp. 104-108. (doi: 10.1016/j.sbsr.2016.10.004) (PMID:28424755) (PMCID:PMC5384990)
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Donnelly, H. , Smith, C.-A. , Sweeten, P. E., Gadegaard, N. , Meek, D., D'Este, M., Mata, A., Eglin, D. and Dalby, M. J. (2017) Bone and cartilage differentiation of a single stem cell population driven by material interface. Journal of Tissue Engineering, 8, pp. 1-10. (doi: 10.1177/2041731417705615) (PMID:28567273) (PMCID:PMC5438107)
Messerschmidt, M., Greer, A. , Schlachter, F., Barnett, J., Thesen, M. W., Gadegaard, N. , Grutzner, G. and Schleunitz, A. (2017) New organic photo-curable nanoimprint resist ≪mr-NIL210≫ for high volume fabrication applying soft PDMS-based stamps. Journal of Photopolymer Science and Technology, 30(5), pp. 605-611. (doi: 10.2494/photopolymer.30.605)
Rostam, H. M., Reynolds, P. M. , Alexander, M. R., Gadegaard, N. and Ghaemmaghami, A. M. (2017) Image based machine learning for identification of macrophage subsets. Scientific Reports, 7, 3521. (doi: 10.1038/s41598-017-03780-z) (PMID:28615717) (PMCID:PMC5471192)
Huethorst, E., Hortigon, M. , Zamora-Rodriguez, V., Reynolds, P.M. , Burton, F., Smith, G. and Gadegaard, N. (2016) Enhanced human-induced pluripotent stem cell derived cardiomyocyte maturation using a dual microgradient substrate. ACS Biomaterials Science and Engineering, 2(12), pp. 2231-2239. (doi: 10.1021/acsbiomaterials.6b00426) (PMID:27990488) (PMCID:PMC5155309)
Greer, A. I.M. , Della-Rosa, B., Khokhar, A. Z. and Gadegaard, N. (2016) Step-and-repeat nanoimprint-, photo- and laser-lithography from one customised CNC machine. Nanoscale Research Letters, 11, 129. (doi: 10.1186/s11671-016-1341-9) (PMID:26956598) (PMCID:PMC4783314)
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Colquhoun, R., Gadegaard, N. , Healy, D. M. and Tanner, K. E. (2016) Characterisation of CorGlaes® Pure 107 fibres for biomedical applications. Journal of Materials Science: Materials in Medicine, 27, 149. (doi: 10.1007/s10856-016-5752-z)
Jack, C., Karimullah, A. S. , Leyman, R., Tullius, R., Rotello, V. M., Cooke, G. , Gadegaard, N. , Barron, L. D. and Kadodwala, M. (2016) Biomacromolecular stereostructure mediates mode hybridization in chiral plasmonic nanostructures. Nano Letters, 16(9), pp. 5806-5814. (doi: 10.1021/acs.nanolett.6b02549) (PMID:27547978)
Aguayo, S., Strange, A., Gadegaard, N. , Dalby, M.J. and Bozec, L. (2016) Influence of biomaterial nanotopography on the adhesive and elastic properties of Staphylococcus aureus cells. RSC Advances, 6(92), pp. 89347-89355. (doi: 10.1039/C6RA12504B)
Stormonth-Darling, J. M. , Saeed, A. , Reynolds, P. M. and Gadegaard, N. (2016) Injection molding micro- and nanostructures in thermoplastic elastomers. Macromolecular Materials and Engineering, 301(8), pp. 964-971. (doi: 10.1002/mame.201600011) (PMID:31217727) (PMCID:PMC6563430)
Ngandu Mpoyi, E., Cantini, M. , Reynolds, P. M. , Gadegaard, N. , Dalby, M. J. and Salmerón-Sánchez, M. (2016) Protein adsorption as a key mediator in the nanotopographical control of cell behavior. ACS Nano, 10(7), pp. 6638-6647. (doi: 10.1021/acsnano.6b01649) (PMID:27391047) (PMCID:PMC4980054)
Greer, A. I.M. , Vasiev, I., Della-Rosa, B. and Gadegaard, N. (2016) Fluorinated ethylene-propylene: a complementary alternative to PDMS for nanoimprint stamps. Nanotechnology, 27(15), 155301. (doi: 10.1088/0957-4484/27/15/155301) (PMID:26938810)
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Greer, A. I. M. , Lim, T. S., Brydone, A. S. and Gadegaard, N. (2016) Mechanical compatibility of sol–gel annealing with titanium for orthopaedic prostheses. Journal of Materials Science: Materials in Medicine, 27, 21. (doi: 10.1007/s10856-015-5611-3) (PMID:26691162) (PMCID:PMC4686541)
Holzmann Rasmussen, C., Reynolds, P. , Petersen, D. R., Hansson, M., McMeeking, R. M., Dufva, M. and Gadegaard, N. (2015) Enhanced differentiation of human embryonic stem cells towards definitive endoderm on ultrahigh aspect ratio nanopillars. Advanced Functional Materials, 26(6), pp. 815-823. (doi: 10.1002/adfm.201504204)
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Gupta, S., Hannah, S., Watson, C.P., Šutta, P., Pedersen, R.H., Gadegaard, N. and Gleskova, H. (2015) Ozone oxidation methods for aluminum oxide formation: Application to low-voltage organic transistors. Organic Electronics, 21, pp. 132-137. (doi: 10.1016/j.orgel.2015.03.007)
Chong, D.S.T., Turner, L.-A., Gadegaard, N. , Seifalian, A.M., Dalby, M.J. and Hamilton, G. (2015) Nanotopography and plasma treatment: redesigning the surface for vascular graft endothelialisation. European Journal of Vascular and Endovascular Surgery, 49(3), pp. 335-343. (doi: 10.1016/j.ejvs.2014.12.008) (PMID:25579872)
Pedersen, R. H., Xu, Q., Stormonth-Darling, J. M. and Gadegaard, N. (2015) Strategies for high quality injection moulding of polymer nanopillars. Macromolecular Materials and Engineering, 300(2), pp. 172-180. (doi: 10.1002/mame.201400134)
Greer, A. I. M. and Gadegaard, N. (2015) A novel, organic, UV-sensitive resist ideal for nanoimprint-, photo- and laser lithography in an air atmosphere. Electronic Materials Letters, 11(4), pp. 544-551. (doi: 10.1007/s13391-015-4401-x)
Pemberton, G. D., Childs, P., Reid, S., Nikukar, H., Tsimbouri, M. , Gadegaard, N. , Curtis, A. S. and Dalby, M. J. (2015) Nanoscale stimulation of osteoblastogenesis from mesenchymal stem cells: nanotopography and nanokicking. Nanomedicine, 10(4), pp. 547-560. (doi: 10.2217/nnm.14.134) (PMID:25723089)
Young, P.S., Tsimbouri, P.M. , Gadegaard, N. , Meek, R.M.D. and Dalby, M.J. (2015) Osteoclastogenesis/osteoblastogenesis using human bone marrow-derived cocultures on nanotopographical polymer surfaces. Nanomedicine, 10(6), pp. 949-957. (doi: 10.2217/nnm.14.146) (PMID:25867859)
Wang, J. R., Ahmed, S. F. , Gadegaard, N. , Meek, R.M. D., Dalby, M. J. and Yarwood, S. J. (2014) Nanotopology potentiates growth hormone signalling and osteogenesis of mesenchymal stem cells. Growth Hormone and IGF Research, 24(6), pp. 245-250. (doi: 10.1016/j.ghir.2014.10.003) (PMID:25466909)
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Yang, J., McNamara, L. E., Gadegaard, N. , Alakpa, E. V., Burgess, K. V., Meek, D. and Dalby, M. J. (2014) Nanotopographical induction of osteogenesis through adhesion, bone morphogenic protein co-signaling and regulation of microRNAs. ACS Nano, 8(10), pp. 9941-9953. (doi: 10.1021/nn504767g) (PMID:25227207)
Palacios-Cuesta, M., Vasiev, I., Gadegaard, N. , Rodríguez-Hernández, J. and García, O. (2014) Direct micrometer patterning and functionalization of polymer blend surfaces by using hot embossing. European Polymer Journal, 59, pp. 333-340. (doi: 10.1016/j.eurpolymj.2014.07.020)
Donoghue, P. S., Sun, T., Gadegaard, N. , Riehle, M. O. and Barnett, S. C. (2014) Development of a novel 3D culture system for screening features of a complex implantable device for CNS repair. Molecular Pharmaceutics, 11(7), pp. 2143-2150. (doi: 10.1021/mp400526n) (PMID:24279373) (PMCID:PMC4087043)
Grenci, G., Sovernigo, E., Khokhar, A. Z., Gadegaard, N. , Prasciolu, M. and Tormen, M. (2014) Microfabrication of sharp blazed gratings by a two-step height amplification process based on soft and deep X-ray lithography. Sensors and Actuators A: Physical, 205, pp. 111-118. (doi: 10.1016/j.sna.2013.09.036)
Dalby, M. J. , Gadegaard, N. and Oreffo, R. O.C. (2014) Harnessing nanotopography and integrin–matrix interactions to influence stem cell fate. Nature Materials, 13(6), pp. 558-569. (doi: 10.1038/NMAT3980)
Stormonth-Darling, J. M. , Pedersen, R. H., How, C. and Gadegaard, N. (2014) Injection moulding of ultra high aspect ratio nanostructures using coated polymer tooling. Journal of Micromechanics and Microengineering, 24(7), 075019. (doi: 10.1088/0960-1317/24/7/075019)
Kadodwala, M. , Abdulrahman, N., Syme, C. D. , Jack, C., Barron, L. D., Karimullah, A. and Gadegaard, N. (2013) The origin of off–resonance non-linear optical activity of a gold chiral nanomaterial. Nanoscale, 5(24), pp. 12651-12657. (doi: 10.1039/c3nr04746f)
Greer, A. I.M. , Seunarine, K., Khokhar, A. Z., MacLaren, I. , Brydone, A. S., Moran, D. A.J. and Gadegaard, N. (2013) Increased efficiency of direct nanoimprinting on planar and curved bulk titanium through surface modification. Microelectronic Engineering, 112, pp. 67-73. (doi: 10.1016/j.mee.2013.05.016)
Hansen, A.M., Sewell, A.L., Pedersen, R.H., Long, D.-L. , Gadegaard, N. and Marquez, R. (2013) Tunable BODIPY derivatives amenable to "click" and peptide chemistry. Tetrahedron, 69(39), pp. 8527-8533. (doi: 10.1016/j.tet.2013.05.037)
Vasiev, I., Greer, A.I.M. , Khokhar, A.Z., Stormonth-Darling, J. , Tanner, K.E. and Gadegaard, N. (2013) Self-folding nano- and micropatterned hydrogel tissue engineering scaffolds by single step photolithographic process. Microelectronic Engineering, 108, pp. 76-81. (doi: 10.1016/j.mee.2013.04.003)
Kingham, E., White, K., Gadegaard, N. , Dalby, M.J. and Oreffo, R.O.C. (2013) Nanotopographical cues augment mesenchymal differentiation of human embryonic stem cells. Small, 9(12), pp. 2140-2151. (doi: 10.1002/smll.201202340)
Tsimbouri, P. M. , Murawski, K., Hamilton, G., Herzyk, P. , Oreffo, R. O.C., Gadegaard, N. and Dalby, M. J. (2013) A genomics approach in determining nanotopographical effects on MSC phenotype. Biomaterials, 34(9), pp. 2177-2184. (doi: 10.1016/j.biomaterials.2012.12.019) (PMID:23312853) (PMCID:PMC3573234)
Reynolds, P.M. , Pedersen, R.H., Stormonth-Darling, J., Dalby, M.J. , Riehle, M.O. and Gadegaard, N. (2013) Label-free segmentation of co-cultured cells on a nanotopographical gradient. Nano Letters, 13(2), pp. 570-576. (doi: 10.1021/nl304097p)
Donoghue, P.S., Lamond, R., Boomkamp, S.D., Tao, S., Gadegaard, N. , Riehle, M.O. and Barnett, S.C. (2013) The development of a ε-polycaprolactone (PCL) scaffold for CNS repair. Tissue Engineering Part A, 19(3), (doi: 10.1089/ten.TEA.2012.0382) (PMID:22994455)
Karimullah, A. S. , Cumming, D. R.S. , Riehle, M. and Gadegaard, N. (2013) Development of a conducting polymer cell impedance sensor. Sensors and Actuators B: Chemical, 176, pp. 667-674. (doi: 10.1016/j.snb.2012.09.075)
Roach, P., Parker, T., Gadegaard, N. and Alexander, M. R. (2013) A bio-inspired neural environment to control neurons comprising radial glia, substrate chemistry and topography. Biomaterials Science, 1, pp. 83-93. (doi: 10.1039/C2BM00060A)
Sun, T., Donoghue, P.S., Higginson, J.R., Gadegaard, N. , Barnett, S.C. and Riehle, M.O. (2013) A miniaturized bioreactor system for the evaluation of cell interaction with designed substrates in perfusion culture. Journal of Tissue Engineering and Regenerative Medicine, 6(S3), S4-S14. (doi: 10.1002/term.510) (PMID:22170765)
Tsimbouri, P.M. , McMurray, R.J., Burgess, K.V., Alakpa, E.V., Reynolds, P. , Murawski, K., Kingham, E., Oreffo, R.O.C., Gadegaard, N. and Dalby, M.J. (2012) Using nanotopography and metabolomics to identify biochemical effectors of multipotency. ACS Nano, 6(11), pp. 10239-10249. (doi: 10.1021/nn304046m)
Stormonth-Darling, J.M. and Gadegaard, N. (2012) Injection moulding difficult nanopatterns with hybrid polymer inlays. Macromolecular Materials and Engineering, 297(11), pp. 1075-1080. (doi: 10.1002/mame.201100397)
Pedersen, R.H., Kustanovich, K. and Gadegaard, N. (2012) Single-step 3D nanolithography using plasma polymerized hexane films. Microelectronic Engineering, 98, pp. 167-170. (doi: 10.1016/j.mee.2012.07.054)
Reynolds, P.M. , Pedersen, R.H., Riehle, M.O. and Gadegaard, N. (2012) A dual gradient assay for the parametric analysis of cell-surface interactions. Small, 8(16), pp. 2541-2547. (doi: 10.1002/smll.201200235)
Pedersen, R.H., Scurr, D.J., Roach, P., Alexander, M.R. and Gadegaard, N. (2012) Full-thickness characterization of plasma polymerized hexane films irradiated by an electron beam. Plasma Processes and Polymers, 9(1), pp. 22-27. (doi: 10.1002/ppap.201100067)
Abdulrahman, N.A., Fan, Z., Tonooka, T., Kelly, S.M. , Gadegaard, N. , Hendry, E., Govorov, A.O. and Kadodwala, M. (2012) Induced chirality through electromagnetic coupling between chiral molecular layers and plasmonic nanostructures. Nano Letters, 12(2), pp. 977-983. (doi: 10.1021/nl204055r)
Greer, A.I.M. , Seunarine, K., Khokhar, A., Li, X. , Moran, D. and Gadegaard, N. (2012) Direct nanopatterning of commercially pure titanium with ultra-nanocrystalline diamond stamps. Physica Status Solidi A: Applications and Materials Science, 209(9), pp. 1721-1725. (doi: 10.1002/pssa.201200057)
Reboud, V. et al. (2012) Enhanced light extraction in ITO-free OLEDs using double-sided printed electrodes. Nanoscale, 4(11), p. 3495. (doi: 10.1039/c2nr12068b)
Kirchhof, K., Andar, A., Yin, H.B. , Gadegaard, N. , Riehle, M.O. and Groth, T. (2011) Polyelectrolyte multilayers generated in a microfluidic device with pH gradients direct adhesion and movement of cells. Lab on a Chip, 11(19), pp. 3326-3335. (doi: 10.1039/C1LC20408D)
McMurray, R.J., Gadegaard, N. , Tsimbouri, P.M. , Burgess, K.V., McNamara, L.E., Tare, R., Murawski, K., Kingham, E., Oreffo, R.O.C. and Dalby, M.J. (2011) Nanoscale surfaces for the long-term maintenance of mesenchymal stem cell phenotype and multipotency. Nature Materials, 10(8), pp. 637-644. (doi: 10.1038/nmat3058) (PMID:21765399)
Kantawong, F., Robertson, M.E., Gadegaard, N. , Oreffo, R.O.C., Burchmore, R.J. and Dalby, M.J. (2011) Protein expression of STRO-1 cells in response to different topographic features. Journal of Tissue Engineering, 2011, 534603. (doi: 10.4061/2011/534603) (PMID:21772957) (PMCID:PMC3136090)
Kingham, E.J., Tsimbouri, P.M. , Gadegaard, N. , Dalby, M.J. and Oreffo, R.O.C. (2011) Nanotopography induced osteogenic differentiation of human stem cells. Bone, 48(Sup. 2), S108-S109. (doi: 10.1016/j.bone.2011.03.177)
Sun, T., Donoghue, P.S., Higginson, J.R., Gadegaard, N. , Barnett, S.C. and Riehle, M.O. (2011) The interactions of astrocytes and fibroblasts with defined pore structures in static and perfusion cultures. Biomaterials, 32(8), pp. 2021-2031. (doi: 10.1016/j.biomaterials.2010.11.046) (PMID:21163522) (PMCID:PMC3440599)
Khokhar, A.Z., Gaston, A., Obieta, I. and Gadegaard, N. (2011) Compact LED based nanoimprinter for UV-NIL. Microelectronic Engineering, 88(11), pp. 3347-3352. (doi: 10.1016/j.mee.2011.06.023)
Tsimbouri, P. , Gadegaard, N. , Burgess, K., Kingham, E., Oreffo, R.O.C. and Dalby, M.J. (2011) Mechanotransduction and skeletal stem cell differentiation in response to bioactive nanotopography. Histology and Histopathology: Cellular and Molecular Biology, 26(S1), 31.012.
Tsimbouri, P. , Gadegaard, N. , Oreffo, R.O.C. and Dalby, M.J. (2011) Effects of nanotopography on cell adhesion, morphology and differentiation. European Cells and Materials, 22(S2), p. 14.
Csaderova, L., Martines, E., Seunarine, K., Gadegaard, N. , Wilkinson, C.D.W. and Riehle, M.O. (2010) A biodegradable and biocompatible regular nanopattern for large-scale selective cell growth. Small, 6(23), pp. 2755-2761. (doi: 10.1002/smll.201000193)
Hendry, E., Carpy, T., Johnston, J., Popland, M., Mikhaylovskiy, R.V., Lapthorn, A. , Kelly, S.M., Barron, L.D., Gadegaard, N. and Kadodwala, M. (2010) Ultrasensitive detection and characterization of biomolecules using superchiral fields. Nature Nanotechnology, 5(11), pp. 783-787. (doi: 10.1038/nnano.2010.209)
Altomare, L., Gadegaard, N. , Visai, L., Tanzi, M.C. and Fare, S. (2010) Biodegradable microgrooved polymeric surfaces obtained by photolithography for skeletal muscle cell orientation and myotube development. Acta Biomaterialia, 6(6), pp. 1948-1957. (doi: 10.1016/j.actbio.2009.12.040)
Altomare, L., Riehle, M.O. , Gadegaard, N. , Tanzi, M.C. and Farè, S. (2010) Microcontact printing of fibronectin on a biodegradable polymeric surface for skeletal muscle cell orientation. International Journal of Artificial Organs, 33(8), pp. 535-543.
Gaston, A., Khokhar, A.Z., Bilbao, L., Saez-Martinez, V., Corres, A., Obieta, I. and Gadegaard, N. (2010) Nanopatterned UV curable hydrogels for biomedical applications. Microelectronic Engineering, 87(5-8), pp. 1057-1061. (doi: 10.1016/j.mee.2009.11.089)
Ji, B., Cusack, M. , Freer, A., Dobson, P.S. , Gadegaard, N. and Yin, H. (2010) Control of crystal polymorph in microfluidics using molluscan 28 kDa Ca2+-binding protein. Integrative Biology, 2, pp. 528-535. (doi: 10.1039/c0ib00007h) (PMID:20820629)
Majani, R., Zelzer, M., Gadegaard, N. , Rose, F.R. and Alexander, M.R. (2010) Preparation of Caco-2 cell sheets using plasma polymerised acrylic acid as a weak boundary layer. Biomaterials, 31(26), pp. 6764-6771. (doi: 10.1016/j.biomaterials.2010.05.049)
McMurray, R.J., Gadegaard, N. , Dalby, M.J. , Tsimbouri, P.M. , Maclaine, S., Meek, D., McNamara, L.E., Child, H. and Berry, C.C. (2010) Research highlights. Nanomedicine, 5(10), pp. 1495-1499. (doi: 10.2217/nnm.10.119)
Pedersen, R.H., Hamzah, M., Thoms, S. , Roach, P., Alexander, M.R. and Gadegaard, N. (2010) Electron beam lithography using plasma polymerized hexane as resist. Microelectronic Engineering, 87(5-8), pp. 1112-1114. (doi: 10.1016/j.mee.2009.11.043)
Roach, P., Parker, T., Gadegaard, N. and Alexander, M.R. (2010) Surface strategies for control of neuronal cell adhesion: A review. Surface Science Reports, 65(6), pp. 145-173. (doi: 10.1016/j.surfrep.2010.07.001)
Kantawong, F., Burchmore, R.J.S. , Gadegaard, N. , Oreffo, R.O.C. and Dalby, M.J. (2009) Proteomic analysis of human osteoprogenitor response to disordered nanotopography. Journal of the Royal Society: Interface, (doi: 10.1098/rsif.2008.0447)
Biggs, M.J.P., Richards, R.G., Gadegaard, N. , McMurray, R.J., Affrosman, S., Wilkinson, C.D., Oreffo, R.O.C. and Dalby, M.J. (2009) Interactions with nanoscale topography: adhesion quantification and signal transduction in cells of osteogenic and multipotent lineage. Journal of Biomedical Materials Research Part A, 91A(1), pp. 195-208. (doi: 10.1002/jbm.a.32196)
Biggs, M.J.P., Richards, R.G., Gadegaard, N. , Wilkinson, C.D.W., Oreffo, R.O.C. and Dalby, M.J. (2009) The use of nanoscale topography to modulate the dynamics of adhesion formation in primary osteoblasts and ERK/MAPK signalling in STRO-1+ enriched skeletal stem cells. Biomaterials, 30(28), pp. 5094-5103. (doi: 10.1016/j.biomaterials.2009.05.049)
Malm, J., Giannaras, D., Riehle, M.O. , Gadegaard, N. and Sjovall, P. (2009) Fixation and drying protocols for the preparation of cell samples for time-of-flight secondary ion mass spectrometry analysis. Analytical Chemistry, 81(17), pp. 7107-7205. (doi: 10.1021/ac900636v)
Kantawong, F., Burgess, K.E.V., Jayawardena, K., Hart, A., Burchmore, R.J. , Gadegaard, N. , Oreffo, R.O.C. and Dalby, M.J. (2009) Whole proteome analysis of osteoprogenitor differentiation induced by disordered nanotopography and mediated by ERK signalling. Biomaterials, 30(27), pp. 4723-4731. (doi: 10.1016/j.biomaterials.2009.05.040)
Seunarine, K., Curtis, A.S.G., Meredith, D.O., Wilkinson, C.D.W., Riehle, M.O. and Gadegaard, N. (2009) A hierarchical response of cells to perpendicular micro- and nanometric textural cues. IEEE Transactions on NanoBioscience, 8(3), pp. 219-225. (doi: 10.1109/TNB.2009.2016477)
Streb, C., Tsunashima, R., MacLaren, D.A. , McGlone, T., Akutagawa, T., Nakamura, T., Scandurra, A., Pignataro, B., Gadegaard, N. and Cronin, L. (2009) Supramolecular silver polyoxometalate architectures direct the growth of composite semiconducting nanostructures. Angewandte Chemie (International Edition), 48(35), pp. 6490-6493. (doi: 10.1002/anie.200901650)
Rossi, L., Riehle, M.O. , McNamara, L.E., Burchmore, R. , Dalby, M.J. , McMurray, R.J., Gadegaard, N. , Ahmed, S. and Tsimbouri, P.M. (2009) Research highlights. Nanomedicine, 4(6), pp. 613-617. (doi: 10.2217/nnm.09.46)
Glidle, A., Hillman, A.R., Rydeo, K.S., Smith, E.L., Cooper, J.M. , Gadegaard, N. , Webster, J.R.P., Dalgliesh, R. and Cubitt, R. (2009) Use of neutron reflectivity to measure the dynamics of solvation and structural changes in polyvinylferrocene films during electrochemically controlled redox cycling. Langmuir, 25(7), pp. 4093-4103. (doi: 10.1021/la803234e)
Krasa, D., Wilkinson, C.D.W., Gadegaard, N. , Kong, X., Zhou, H., Roberts, A.P., Muxworthy, A.R. and Williams, W. (2009) Nanofabrication of two-dimensional arrays of magnetite particles for fundamental rock magnetic studies. Journal of Geophysical Research: Solid Earth, 114, B02104. (doi: 10.1029/2008JB006017)
Lamond, R., Donoghue, P., Gadegaard, N. , Riehle, M. and Barnett, S. (2009) Developing a biodegradable construct to enhance axonal alignment following a spinal cord injury. Glia, 57(13), S162-S162.
MacLaren, D.A. , Johnston, J., Duncan, D.A., Marchetto, H., Dhesi, S.S., Gadegaard, N. and Kadodwala, M. (2009) Asymmetric photoelectron transmission through chirally-sculpted, polycrystalline gold. Physical Chemistry Chemical Physics, 11(38), pp. 8413-8416. (doi: 10.1039/b911447e)
Song, Y.F., McMillan, N., Long, D.L. , Kane, S., Malm, J., Riehle, M. , Pradeep, C.P., Gadegaard, N. and Cronin, L. (2009) Micropatterned Surfaces with Covalently Grafted Unsymmetrical Polyoxometalate-Hybrid Clusters Lead to Selective Cell Adhesion. Journal of the American Chemical Society, 131(4), pp. 1340-1341. (doi: 10.1021/ja807091v)
Yang, J., Rose, F.R.A.J., Gadegaard, N. and Alexander, M. (2009) A high-throughput assay of cell-surface interactions using topographical and chemical gradients. Advanced Materials, 21(3), pp. 300-304. (doi: 10.1002/adma.200801942)
Yang, J., Rose, F.R.A.J., Gadegaard, N. and Alexander, M.R. (2009) Effect of sessile drop volume on the wetting anisotropy observed on grooved surfaces. Langmuir, 25(5), pp. 2567-2571. (doi: 10.1021/la803942h)
Yin, H. , Bozhi, J., Dobson, P.S. , Mosbahi, K., Glidle, A., Gadegaard, N. , Freer, A., Cooper, J.M. and Cusack, M. (2009) Screening of biomineralization using microfluidics. Analytical Chemistry, 81(1), pp. 473-478. (doi: 10.1021/ac801980b) (PMID:19117468)
Zelzer, M., Scurr, D., Abdullah, B., Urquhart, A.J., Gadegaard, N. , Bradley, J.W. and Alexander, M.R. (2009) Influence of the plasma sheath on plasma polymer deposition in advance of a mask and down pores. Journal of Physical Chemistry B, 113(25), pp. 8487-8494. (doi: 10.1021/jp902137y)
Dalby, M.J. , McNamara, L.E., Riehle, M.O. , Burchmore, R.J.S. , Kuntanawat, P., McMurray, R.J., Gadegaard, N. and Andar, A. (2008) Research highlights. Nanomedicine, 3(4), pp. 411-414. (doi: 10.2217/17435889.3.4.411)
Fleming, C., Long, D. L. , McMillan, N., Johnston, J., Bovet, N., Dhanak, V., Gadegaard, N. , Kogerler, P., Cronin, L. and Kadodwala, M. (2008) Reversible electron-transfer reactions within a nanoscale metal oxide cage mediated by metallic substrates. Nature Nanotechnology, 3(4), pp. 229-233. (doi: 10.1038/nnano.2008.66)
Song, Y.-F., McMillan, N., Long, D.-L. , Thiel, J., Ding, Y., Chen, H., Gadegaard, N. and Cronin, L. (2008) Design of hydrophobic polyoxometalate hybrid assemblies beyond surfactant encapsulation. Chemistry: A European Journal, 14(8), pp. 2349-2354. (doi: 10.1002/chem.200701629)
Dalby, M.J. , Gadegaard, N. and Wilkinson, C.D.W. (2008) The response of fibroblasts to hexagonal nanotopography fabricated by electron beam lithography. Journal of Biomedical Materials Research Part A, 84A(4), pp. 973-979. (doi: 10.1002/jbm.a.31409)
Gadegaard, N. , Chen, X.Y., Rutten, F.J.M. and Alexander, M.R. (2008) High-energy electron beam lithography of octadecylphosphonic acid monolayers on aluminum. Langmuir, 24(5), pp. 2057-2063. (doi: 10.1021/la701733n)
Gadegaard, N. , Dalby, M.J. , Riehle, M.O. and Wilkinson, C.D.W. (2008) Optimizing substrate disorder for bone tissue engineering of mesenchymal stem cells. Journal of Vacuum Science and Technology B: Microelectronics and Nanometer Structures, 26(6), pp. 2554-2557. (doi: 10.1116/1.2978407)
Gadegaard, N. , Seunarine, K., Smith, D.J.A., Meredith, D., Wilkinson, C.D.W. and Riehle, M.O. (2008) A hybrid three-dimensional nanofabrication method for producing vascular tissue engineering scaffold. Japanese Journal of Applied Physics, 47(9), pp. 7415-7419. (doi: 10.1143/JAPP.47.7415)
Seunarine, K., Meredith, D.O., Riehle, M.O. , Wilkinson, C.D.W. and Gadegaard, N. (2008) Biodegradable polymer tubes with litho graphically controlled 3D micro- and nanotopography. Microelectronic Engineering, 85(5-6), pp. 1350-1354. (doi: 10.1016/j.mee.2008.02.002)
Bovet, N., McMillan, N., Gadegaard, N. and Kadodwala, M. (2007) Supramolecular assembly facilitating adsorbate-induced chiral electronic states in a metal surface. Journal of the American Chemical Society, 111(33), pp. 10005-10011. (doi: 10.1021/jp074056s)
Biggs, M.J.P., Richards, R.G., Gadegaard, N. , Wilkinson, C.D.W. and Dalby, M.J. (2007) The effects of nanoscale pits on primary human osteoblast adhesion formation and cellular spreading. Journal of Materials Science: Materials in Medicine, 18, pp. 399-404. (doi: 10.1007/s10856-006-0705-6)
Biggs, M.J.P., Richards, R.G., Gadegaard, N. , Wilkinson, C.D.W. and Dalby, M.J. (2007) Regulation of implant surface cell adhesion: Characterization and quantification of S-phase primary osteoblast adhesions on biomimetic nanoscale substrates. Journal of Orthopaedic Research, 25, pp. 273-282. (doi: 10.1002/jor.20319)
Dalby, M.J. , Biggs, M.J.P., Gadegaard, N. , Kalna, G., Wilkinson, C.D.W. and Curtis, A.S.G. (2007) Nanotopographical stimulation of mechanotransduction and changes in interphase centromere positioning. Journal of Cellular Biochemistry, 100, pp. 326-338. (doi: 10.1002/jcb.21058)
Dalby, M.J. , Gadegaard, N. , Herzyk, P. , Agheli, H., Sutherland, D.S. and Wilkinson, C.D.W. (2007) Group analysis of regulation of fibroblast genome on low-adhesion nanostructures. Biomaterials, 28, pp. 1761-1769. (doi: 10.1016/j.biomaterials.2006.11.049)
Dalby, M.J. , Gadegaard, N. , Tare, R., Andar, A., Riehle, M.O. , Herzyk, P. , Wilkinson, C.D.W. and Oreffo, R.O.C. (2007) The control of human mesenchymal cell differentiation using nanoscale symmetry and disorder. Nature Materials, 6(12), pp. 997-1003. (doi: 10.1038/nmat2013)
McMillan, N., Smith, L.V., de la Fuente, J.M., Parenty, A.D.C., Gadegaard, N. , Pitt, A.R., Thomson, K., MacKenzie, C., Kelly, S. and Cronin, L. (2007) Incorporation of N-heterocyclic cations into proteins with a highly directed chemical modification. Chemical Communications, 25, pp. 2581-2583. (doi: 10.1039/b702575k)
Song, Y.-F., Abbas, H., Ritchie, C., McMillian, N., Long, D.-L. , Gadegaard, N. and Cronin, L. (2007) From polyoxometalate building blocks to polymers and materials: the silver connection. Journal of Materials Chemistry, 17, pp. 1903-1908. (doi: 10.1039/b617830h)
Curtis, A.S.G., Dalby, M.J. and Gadegaard, N. (2006) Cell signaling arising from nanotopography: implications for nanomedical devices. Nanomedicine, 1, pp. 67-72. (doi: 10.2217/17435889.1.1.67)
Gadegaard, N. (2006) Atomic force microscopy in biology: technology and techniques. Biotechnic and Histochemistry, 81, pp. 87-97. (doi: 10.1080/10520290600783143)
Pranov, H., Rasmussen, H., Larsen, N. and Gadegaard, N. (2006) On the injection molding of nanostructured polymer surfaces. Polymer Engineering and Science, 46, pp. 160-171. (doi: 10.1002/pen.20459)
Seunarine, K., Gadegaard, N. , Tormen, M., O Meredith, D., O Riehle, M. and Wilkinson, C. (2006) 3D polymer scaffolds for tissue engineering. Nanomedicine, 1, pp. 281-296. (doi: 10.2217/17435889.1.3.281)
Seunarine, K., Tormen, M., Gadegaard, N. , Riehle, M. , Wilkinson, C.D.W., Businaro, L. and Romanato, F. (2006) Progress towards tubes with regular nanopatterned inner surfaces. Journal of Vacuum Science and Technology B: Microelectronics and Nanometer Structures, 24(6), pp. 3258-3262. (doi: 10.1116/1.2357970)
Glidle, A., Hadyoon, C.S., Gadegaard, N. , Cooper, J.M. , Hillman, A.R., Wilson, A.R., Ryder, K.S., Webster, J.R.P. and Cubitt, R. (2005) Evaluating the influence of deposition conditions on solvation of reactive conducting polymers with neutron reflectivity. Journal of Physical Chemistry B, 109(30), pp. 14335-14343. (doi: 10.1021/jp0515030)
Gadegaard, N. , Dalby, M.J. , Riehle, M.O. , Curtis, A.S.G. and Affrossman, S. (2004) Tubes with controllable internal nanotopography. Advanced Materials, 20, pp. 1857-1860. (doi: 10.1002/adma.200400408)
Dalby, M.J. , Gadegaard, N. , Riehle, M.O. , Wilkinson, C.D.W. and Curtis, A.S.G. (2004) Investigating filopodia sensing using arrays of defined nano-pits down to 35 nm diameter in size. International Journal of Biochemistry and Cell Biology, 36(10), pp. 2005-2015. (doi: 10.1016/j.biocel.2004.03.001)
Dalby, M.J. , Giannaras, D., Riehle, M.O. , Gadegaard, N. , Affrossman, S. and Curtis, A.S.G. (2004) Rapid fibroblast adhesion to 27 nm high polymer demixed nano-topography. Biomaterials, 25(1), pp. 77-83. (doi: 10.1016/S0142-9612(03)00475-7)
Cooper, J.M. , Cubitt, R., Dalgliesh, R.M., Gadegaard, N. , Glidle, A., Hillman, A.R., Mortimer, R.J., Ryder, K.S. and Smith, E.L. (2004) Dynamic in situ electrochemical neutron reflectivity measurements. Journal of the American Chemical Society, 126(47), pp. 15362-15363. (doi: 10.1021/ja044682s)
Curtis, A.S.G., Gadegaard, N. , Dalby, M.J. , Riehle, M.O. , Wilkinson, C.D.W. and Aitchison, G. (2004) Cells react to nanoscale order and symmetry in their surroundings. IEEE Transactions on NanoBioscience, 3(1), pp. 61-65. (doi: 10.1109/TNB.2004.824276)
Gadegaard, N. , Thoms, S., Macintyre, D., Mcghee, K., Gallagher, J., Casey, B. and Wilkinson, C. (2003) Arrays of nano-dots for cellular engineering. Microelectronic Engineering, 67-8, pp. 162-168. (doi: 10.1016/S0167-9317(03)00067-4)
Wilkinson, C.D.W., Thoms, S. , Macintyre, D., Curtis, A.S.G., Riehle, M. and Gadegaard, N. (2003) Nanofabrication of structures for cell engineering. Proceedings of the SPIE: The International Society for Optical Engineering, 5220, pp. 1-9. (doi: 10.1117/12.509749)
Hillman, A.R., Bailey, L., Glidle, A., Cooper, J.M. , Gadegaard, N. and Webster, J.R.P. (2002) Spatial distributions of polymer and mobile species in poly(o-toluidine) films. Journal of Electroanalytical Chemistry, 532, pp. 269-276.
Book Sections
Reynolds, P. M. and Gadegaard, N. (2015) Polymer gradient surfaces for biomedical applications. In: Rodríguez-Hernández,, J. and Cortajarena, A. L. (eds.) Design of Polymeric Platforms for Selective Biorecognition. Springer International Publishing, pp. 93-122. ISBN 9783319170602 (doi: 10.1007/978-3-319-17061-9_5)
Research Reports or Papers
Ivanova, Y., Aitchison, I., Cannon, P. , Butler, D., Gadegaard, N. and Keith, N. (2020) Mapping of University Research Output and Impact to the United Nations Sustainable Development Goals. Project Report. OSF. (doi: 10.17605/OSF.IO/UFYTA).
Conference or Workshop Item
Kumar, C. , Laughtland, K., Bairagi, S., Gadegaard, N. and Mulvihill, D. (2023) Mechanics of Sliding Triboelectric Nanogenerators for Sustainable Energy Harvesting. 17th International Conference on Advances in Experimental Mechanics, Glasgow University Union, University of Glasgow, UK, 30 August 2023 - 01 September 2023.
Conference Proceedings
Young, P.S., Greer, A.I.M. , Tsimbouri, P.M. , Meek, R.M.D., Gadegaard, N. and Dalby, M.J. (2016) Precision-Engineered Nanotopographical Surfaces to Control Osteoclast Differentiation. In: The British Orthopaedic Research Society (BORS) Annual Conference, Glasgow, UK, 5-6 Sept 2016,
Greer, A. I. M. , Seunarine, K., Khokhar, A. Z., Li, X. , Moran, D. A. J. and Gadegaard, N. (2012) Direct Nano-Patterning of Commercially Pure Titanium with Ultra-Nanocrystalline Diamond Stamps. In: SBDD XVII Diamond Workshop, Hasselt, Belgium, March 2012,
Khokhar, A.Z., Tsimbouri, P.M. , Dalby, M.J. and Gadegaard, N. (2011) Development of ultra-hard stamps for titanium patterning. In: 37th International Conference on Micro and Nano Engineering: MNE 2011, Berlin, 19 - 23 September 2011,
Obieta, I., Bilbao, L., Corres, A., Gadegaard, N. , Khokhar, A.Z. and Gadegaard, N. (2011) 3D nanopatterning of photosensitive hydrogels. In: NanoSpain Conference 2011, Bilbao, Spain, 11-14 April 2011,
Grants
FAKIR, ERC; 2015-2020
Mapping the mesoscale structural landscape using "sculpted" chiral plasmonic fields, EPSRC; 2016-2020
NWCAM-North West Centre for Advanced Manufacturing, European Commission, 2017-2022
Hybrid Technology Hub, Norwegian Research Council, 2017-2027
Microengineered joints, Leverhulme Trust, 2018-2022
Cellular Mechanics, CRUK, 2018-2020
Meta-chemistry, EPSRC, 2019-2022
Meta-smart, EPSRC, 2019-2022
Mega FLIM, EPSRC, 2019-2022
Supervision
Research Assistants
Marie Cutiongco
Badri Aekbote
Chris Syme
Andrew Greer
Miriam Rol Garcia
PhD students
Emma Barbour
Neil Convery
Fraser Campbell
Eline Huethorst
Anna Koester
Alice Main
Alex Hamilton
James Mordue
Jack Perris
Roderick McNeill
Iliyana Samardzhieva
- Lyall, Maia
Single cell forces as a marker in discovery and treatment of vascular diseases
Teaching
Biosensors and Diagnostics 4 (ENG4036) - Course coordinator
Scaffolds and Tissues M (ENG5282) - Course coordinator
Introduction to Research in Nanoscience and Nanotechnology (ENG5144)
Research datasets
2020
Ross, E., Turner, L.-A., Saeed, A., DONNELLY, H., Burgess, K., Blackburn, G., Reynolds, P., Wells, J., Mountford, J., Gadegaard, N. , Oreffo, R., Salmeron-Sanchez, M. and Dalby, M. (2020) Nanotopography reveals metabolites that maintain the immunomodulatory phenotype of mesenchymal stromal cells during large-scale expansion. [Data Collection]
Jensen, B. , Cutiongco, M., Reynolds, P. and Gadegaard, N. (2020) Predicting gene expression using morphological cell responses to nanotopography. [Data Collection]
2019
DONNELLY, H., Smith, C.-A., Sweeten, P., Gadegaard, N. , Meek, R. M. D., D'Este, M., Mata, A., Eglin, E. and Dalby, M. (2019) Bone and cartilage differentiation of a single stem cell population driven by material interface. [Data Collection]
2017
Alakpa, E. V., Burgess, K., Chung, P., Riehle, M. , Gadegaard, N. , Dalby, M. and Cusack, M. (2017) Nacre topography produces higher crystallinity in bone than chemically-induced osteogenesis. [Data Collection]
2016
Lee, L., Gadegaard, N. , Andrés, M. C. d., Turner, L.-A., Burgess, K., Yarwood, S., Wells, J., Mpoyi, E., Salmerón-Sánchez, M., Meek, R. D., Oreffo, R. and Dalby, M. (2016) Nanotopography controls cell cycle changes involved with skeletal stem cell self-renewal and multipotency. [Data Collection] (Unpublished)
Ngandu Mpoyi, E., Cantini, M. , Reynolds, P. , Gadegaard, N. , Dalby, M. and Salmerón-Sánchez, M. (2016) Protein adsorption as a key mediator in the nanotopographical control of cell behavior. [Data Collection]
Additional information
Potential PhD projects
Development of a microfluidic probe for high-content single cell analysis
The golden standard in assessment of cellular function and state is by biochemical analysis such as qPCR, ELISA etc. Usually these measurements are based on whole cell populations of 1000s to 1000000s of cells. As there is a natural variance between cells, it is therefore of interest to analyse samples from smaller populations to single cells. As the analysis generally is acquired from a Petri dish or similar it is difficult to reduce the number of cells without reducing the concentration of the analysis too. However, with the use of a microfluidic probe, it is possible to sample directly in a Petri dish from a select population. Using this method it will be possible to sample from micro-array samples for higher throughput.
In this project you will develop a microfluidic probe platform based on an inverted microscope. This will involve the design of new probes and manipulation mechanics. Once assembled, it will be applied on a range of cell system to validate the process.
The project has partners at IBM Zurich.
Imaging Plasmonic Polarimetry
We have demonstrated the ability to measure the structure of biological molecules using nanopatterned surfaces with unique plasmonic properties. When these plasmonic surfaces are exposed to light, they generate optical activity which is highly sensitive to the surrounding environment. These fields are capable of detecting binding events to proteins and are also able to detect the structural nature of the proteins. We are now able to measure these changes using a reflective polarimeter. In this project the candidate will build and test an imaging system for superpolarimetry which will enable spatial detection from a patterned substrate. Once the instrument has been build it will be calibrated against more established methods and finally tested with known biological samples.
Developing a functional hepatic niche using nanopatterning and hepatic stem cells
Conventional hepatic culture models for drug discovery assays mostly use rodent primary hepatocytes or human immortalized cell lines, which rapidly lose polarity and phenotype and are not representative of normal liver tissue. These models often lack the functional repertoire of primary human hepatocytes (PHHs) including the ability to metabolize drugs (CYP450 activity). However, PHHs have a short culture life-span, exhibit phenotypic variability and instability in culture with intermittent supply and high unit costs. Hence alternative culture models are in great demand and a promising direction is the recent development of HepaRG cells. These are progenitors with the potential to differentiate into the different cell types found in the liver.
The aim of this project is to study the effect of micro- and nanopatterning on a hepatocyte stem cell line, HepaRG. These are human derived cells and are available in a proliferative and differentiated state, depending on what we would like to study. The cells will be cultured and imaged on the difference nanotopographies as well as novel mechanically compliant substrates and their size, density and shape measure together with P450 activity as well as other makers. These parameters will then be correlated with metabolic activity measured using mass spectrometry.
Nanopatterns identified to provide stable hepatocyte cultures will then be used to study the effect paracetamol toxity, another benchmarking compound. The impact on the cultures will be assessed is a similar manner as above. Successful patterns will then be taken forward to study influence on HepaRG cells.
Superresolution and correlative microscopy to study cell adhesion on nanopatterned substrates
As cells adhere to surfaces, they for focal adhesions which link the cells mechanically to their surroundings but also provide important signalling too. With the invention of superresolution microscopy it has become possible to study the proteins involved in these adhesions at a molecular level. The improvement in detail is typically 10 times with a resolution of about 20 nm.
At the same time semiconductor technologies have made it possible to control patterns with a precision similar to the size of the proteins. This opens up the possibilities to potentially manipulate the adhesions at a molecular level. These interactions can be visualised with a combination of superresolution and scanning electron microscopy, known as correlative microscopy.
In this project you will use advanced microscopy and genetically engineered cells to investigate the adhesion formation on a range of nanopatterned surfaces. The aim will be to correlate the formation of the focal adhesions with the design parameters with a view to produce engineered materials for regenerative medicine. This project is part of an ERC funded 5 year programme.
Measuring nanoNewton forces from cancer cells during metastasis
As cells migrate in the body, they exert force on their environment. The magnitude of such forces is in the nanoNewton range and can be measure in vitro (in a Petri dish) in various ways. One method is to have a bed of regularly spaced flexible pillars on which the cells are migrating. By tracking the movement of these pillars and knowing their mechanical properties it is possible to calculate direction and magnitude of forces the cells are exerting on the substrates. This is highly relevant for cancer cells during metastasis.
In this project, the aim is to manufacture such micropillars and study the forces from different cancer cell types. Here cells with varying degrees of malignancy will be measured and correlated to the forces measured. You will also construct a model for intravasation, mimicking the metastasis from the tumour, and measure the force as the cells “squeeze” through the endothelium.
Collaborators on the project are the Beatson Institute, Columbia University (New York) and the Mechanobiology Institute in Singapore.
Development of nanoscale mechanotaxis arrays for cell engineering
There is clear evidence that eukaryotic cells are influence by surface topography. Their response goes from the micrometer range, comparable to a single cell, down into the nanometer range. Over the past 10 years or more, we have demonstrated that highly ordered nanopatterns prepared by electron beam lithography profoundly influence cell adhesion, migration and proteome. More recently we discovered that bone marrow derived stem cells can be made to either differentiate to bone forming cells or retain multipotency in prolonged culture. This is driven by minute changes in the surface topography (<50 nm) and we have shown that the metabolic profiles for the cells on the different surfaces are distinctly different.
The aim of this project will be to develop new substrates with the emphasis on mechanical properties. It is well-known that the mechanical properties of the substrate on which stem cells are seeded will influence their fate. Where most work has been carried out on gels or elastomers, this project will develop a process whereby solid substrate can be manufactured to have similar mechanical properties at the surface.
The project will involve a close collaboration with Columbia University in New York City.
Advanced manufacturing of medical implants and devices
Injection moulding is the preferred technology used to make large quantities of plastic parts - often with complex shape and surface texture. One of the reasons behind the success of the injection moulding process is the speed of manufacture where parts can be made in seconds in a fully automatic process. This is driven by the rapid cooling of the injected polymer into the tool cavity. However, as the complexity of the parts increases, the process is challenged as the polymer may freeze too rapidly to take the shape or dimensions of the tool. As a means to compensate for the rapid cooling yet still benefitting from the automatic process variothermal injection moulding has been developed. Here the tooling is kept about the Tg (glass transition temperature) of the polymer which maintains the liquid state of the polymer. Once the tool is filled it is then cooled below Tg before the parts is removed. This adds additional processing time but enables the manufacture of complex parts.
This project will develop tooling technologies for faster mastering through the use of 3D printing and direct laser writing. The project will also develop variothermal heating on the injection moulding equipment available within the research group. This project will involve collaboration with several companies and international partners.
