Dr Steven Magennis
- Reader in Physical Chemistry (School of Chemistry)
telephone:
+44 (0) 141 330 7339
email:
Steven.Magennis@glasgow.ac.uk
pronouns:
He/him/his
C5-19, School of Chemistry, Joseph Black Building, Glasgow, G12 8QQ
Research interests
Research Interests
We use and develop single-molecule fluorescence techniques to probe the structure, dynamics and reactivity of DNA.
Details of the group's research can be found here.
Research groups
Publications
2024
Magennis, S. W. , Bailie, A. E., Sansom, H. G., Fisher, R. S.S., Watabe, R., Tor, Y. and Jones, A. C. (2024) Ultrasensitive detection of a responsive fluorescent thymidine analogue in DNA via pulse-shaped two-photon excitation. Physical Chemistry Chemical Physics, (doi: 10.1039/D4CP03391D) (In Press)
Cadden, G. M., Wilken, S. J. and Magennis, S. W. (2024) A single CAA interrupt in a DNA three-way junction containing a CAG repeat hairpin results in parity-dependent trapping. Nucleic Acids Research, 52(15), pp. 9317-9327. (doi: 10.1093/nar/gkae644) (PMID:39041420) (PMCID:PMC11347167)
Ploetz, E. et al. (2024) A new twist on PIFE: photoisomerisation-related fluorescence enhancement. Methods and Applications in Fluorescence, 12(1), 012001. (doi: 10.1088/2050-6120/acfb58) (PMID:37726007) (PMCID:PMC10570931)
2023
Dodds, A. C., Sansom, H. G., Magennis, S. W. and Sutherland, A. (2023) Synthesis of Thiazoloindole α‑Amino Acids: Chromophores Amenable to One- and Two-Photon Induced Fluorescence. Organic Letters, 25(49), pp. 8942-8946. (doi: 10.1021/acs.orglett.3c03851) (PMID:38055619) (PMCID:PMC10729019)
Nobis, D., Sansom, H. G. and Magennis, S. W. (2023) Pulse-shaped broadband multiphoton excitation for single-molecule fluorescence detection in the far field. Methods and Applications in Fluorescence, 11(1), 017001. (doi: 10.1088/2050-6120/aca87f)
2022
Bianco, S. , Hu, T., Henrich, O. and Magennis, S. W. (2022) Heterogeneous migration routes of DNA triplet repeat slip-outs. Biophysical Reports, 2(3), 100070. (doi: 10.1016/j.bpr.2022.100070) (PMID:36299495) (PMCID:PMC9586884)
2021
Samaan, G. N., Wyllie, M. K., Cizmic, J. M., Needham, L.-M., Nobis, D., Ngo, K., Andersen, S., Magennis, S. W. , Lee, S. F. and Purse, B. W. (2021) Single-molecule fluorescence detection of a tricyclic nucleoside analogue. Chemical Science, 12(7), pp. 2623-2628. (doi: 10.1039/D0SC03903A) (PMID:34164030) (PMCID:PMC8179283)
Hu, T., Morten, M. J. and Magennis, S. M. (2021) Conformational and migrational dynamics of slipped-strand DNA three-way junctions containing trinucleotide repeats. Nature Communications, 12, 204. (doi: 10.1038/s41467-020-20426-3) (PMID:33420051) (PMCID:PMC7794359)
2020
Morten, M. J., Steinmark, E. and Magennis, S. W. (2020) Probing DNA dynamics: stacking-induced fluorescence increase (SIFI) versus FRET. ChemPhotoChem, 4(9), pp. 664-667. (doi: 10.1002/cptc.202000069)
Berrocal-Martin, R., Sanchez-Cano, C., Chiu, C. K.C., Needham, R. J., Sadler, P. J. and Magennis, S. W. (2020) Metallation‐induced heterogeneous dynamics of DNA revealed by single‐molecule FRET. Chemistry: A European Journal, 26(22), pp. 4980-4987. (doi: 10.1002/chem.202000458) (PMID:31999015)
Berrones‐Reyes, J. C., Muñoz‐Flores, B. M., Uscanga‐Palomeque, A. C., Santillán, R., Del Angel‐Mosqueda, C., Nobis, D., Cochrane, M. A., Magennis, S. and Jiménez‐Pérez, V. M. (2020) Two‐photon detection of organotin Schiff base complexes in cancer cells. ChemistrySelect, 5(5), pp. 1623-1627. (doi: 10.1002/slct.201904816)
Bell, J. D., Harkiss, A. H., Nobis, D., Malcolm, E., Knuhtsen, A., Wellaway, C. R., Jamieson, A. G. , Magennis, S. W. and Sutherland, A. (2020) Conformationally rigid pyrazoloquinazoline α-amino acids: one- and two-photon induced fluorescence. Chemical Communications, 56(12), pp. 1887-1890. (doi: 10.1039/C9CC09064A) (PMID:31956866)
2019
Nobis, D., Fisher, R. S., Simmermacher, M., Hopkins, P. A., Tor, Y., Jones, A. C. and Magennis, S. W. (2019) Single-molecule detection of a fluorescent nucleobase analog via multiphoton excitation. Journal of Physical Chemistry Letters, 10(17), pp. 5008-5012. (doi: 10.1021/acs.jpclett.9b02108) (PMID:31397575)
2018
Morten, M. J., Lopez, S. G., Steinmark, I. E., Rafferty, A. and Magennis, S. W. (2018) Stacking-induced fluorescence increase reveals allosteric interactions through DNA. Nucleic Acids Research, 46(21), pp. 11618-11626. (doi: 10.1093/nar/gky887) (PMID:30277520) (PMCID:PMC6265455)
Bood, M. et al. (2018) Pentacyclic adenine: a versatile and exceptionally bright fluorescent DNA base analog. Chemical Science, 9(14), pp. 3494-3502. (doi: 10.1039/C7SC05448C) (PMID:29780479) (PMCID:PMC5934695)
Baltierra Jasso, L., Morten, M. and Magennis, S. W. (2018) Sub-ensemble monitoring of DNA strand displacement using multiparameter single-molecule FRET. ChemPhysChem, 19(5), pp. 551-555. (doi: 10.1002/cphc.201800012) (PMID:29316151)
Fisher, R. S., Nobis, D., Füchtbauer, A. F., Bood, M., Grøtli, M., Wilhelmsson, L. M., Jones, A. C. and Magennis, S. W. (2018) Pulse-shaped two-photon excitation of a fluorescent base analogue approaches single-molecule sensitivity. Physical Chemistry Chemical Physics, 20(45), pp. 28487-28498. (doi: 10.1039/c8cp05496g) (PMID:30412214)
2017
Venkatesh, V. et al. (2017) Mitochondria-targeted spin-labelled luminescent iridium anticancer complexes. Chemical Science, 8(12), pp. 8271-8278. (doi: 10.1039/C7SC03216A)
Magennis, S. , Toulmin, A., Baltierra-Jasso, L. E., Morten, M. J., Sabir, T., McGlynn, P., Schröder, G. F., Smith, B. O. and Magennis, S. W. (2017) Conformational heterogeneity in a fully-complementary DNA three-way junction with a GC-rich branchpoint. Biochemistry, 56(37), pp. 4985-4991. (doi: 10.1021/acs.biochem.7b00677) (PMID:28820590)
Quinn, S. D. and Magennis, S. W. (2017) Optical detection of gadolinium(III) ions via quantum dot aggregation. RSC Advances, 7(40), pp. 24730-24735. (doi: 10.1039/C7RA03969G) (PMID:29308186) (PMCID:PMC5735353)
2016
Dalton, C. E., Quinn, S. D. , Rafferty, A., Morten, M. J., Gardiner, J. M. and Magennis, S. W. (2016) Single-molecule fluorescence detection of a synthetic heparan sulfate disaccharide. ChemPhysChem, 17(21), pp. 3442-3446. (doi: 10.1002/cphc.201600750) (PMID:27538128) (PMCID:PMC5111599)
Quinn, S. D. , Rafferty, A., Dick, E., Morten, M. J., Kettles, F. J., Knox, C., Murrie, M. and Magennis, S. W. (2016) Surface charge control of quantum dot blinking. Journal of Physical Chemistry C, 120(34), pp. 19487-19491. (doi: 10.1021/acs.jpcc.6b07779)
Baltierra-Jasso, L. E., Morten, M., Laflör, L., Quinn, S. D. and Magennis, S. W. (2016) DNA hairpin dynamics under molecular crowding conditions. Biophysical Journal, 110(3 S1), 634a-635a. (doi: 10.1016/j.bpj.2015.11.3397)
Quinn, S. D. , Dalton, C. E., Jeanneret, R. A., Gardiner, J. M. and Magennis, S. W. (2016) Single-molecule studies of fluorescently-labelled polysaccharides. Biophysical Journal, 110(3 S1), 636a-636a.
2015
Baltierra-Jasso, L. E., Morten, M. J., Laflor, L., Quinn, S. D. and Magennis, S. W. (2015) Crowding-induced hybridization of single DNA hairpins. Journal of the American Chemical Society, 137(51), pp. 16020-16023. (doi: 10.1021/jacs.5b11829) (PMID:26654490)
2014
Lane, R. S. K., Jones, R., Sinkeldam, R. W., Tor, Y. and Magennis, S. W. (2014) Two-photon-induced fluorescence of isomorphic nucleobase analogs. ChemPhysChem, 15(5), pp. 867-871. (doi: 10.1002/cphc.201400031)
2013
Pudney, C.R., Lane, R.S.K., Fielding, A.J., Magennis, S.W. , Hay, S. and Scrutton, N.S. (2013) Enzymatic single-molecule kinetic isotope effects. Journal of the American Chemical Society, 135(10), pp. 3855-3864. (doi: 10.1021/ja309286r)
2012
Sabir, T., Toulmin, A., Ma, L., Jones, A.C., McGlynn, P., Schröder, G.F. and Magennis, S.W. (2012) Branchpoint expansion in a fully complementary three-way DNA junction. Journal of the American Chemical Society, 134(14), pp. 6280-6285. (doi: 10.1021/ja211802z)
2011
Sabir, T., Schröder, G.F., Toulmin, A., McGlynn, P. and Magennis, S.W. (2011) Global structure of forked DNA in solution revealed by high-resolution single-molecule FRET. Journal of the American Chemical Society, 133(5), pp. 1188-1191. (doi: 10.1021/ja108626w)
2008
Channon, K.J., Devlin, G.L., Magennis, S.W. , Finlayson, C.E., Tickler, A.K., Silva, C. and MacPhee, C.E. (2008) Modification of fluorophore photophysics through peptide-driven self-assembly. Journal of the American Chemical Society, 130(16), pp. 5487-5491. (doi: 10.1021/ja710310c)
Articles
Magennis, S. W. , Bailie, A. E., Sansom, H. G., Fisher, R. S.S., Watabe, R., Tor, Y. and Jones, A. C. (2024) Ultrasensitive detection of a responsive fluorescent thymidine analogue in DNA via pulse-shaped two-photon excitation. Physical Chemistry Chemical Physics, (doi: 10.1039/D4CP03391D) (In Press)
Cadden, G. M., Wilken, S. J. and Magennis, S. W. (2024) A single CAA interrupt in a DNA three-way junction containing a CAG repeat hairpin results in parity-dependent trapping. Nucleic Acids Research, 52(15), pp. 9317-9327. (doi: 10.1093/nar/gkae644) (PMID:39041420) (PMCID:PMC11347167)
Ploetz, E. et al. (2024) A new twist on PIFE: photoisomerisation-related fluorescence enhancement. Methods and Applications in Fluorescence, 12(1), 012001. (doi: 10.1088/2050-6120/acfb58) (PMID:37726007) (PMCID:PMC10570931)
Dodds, A. C., Sansom, H. G., Magennis, S. W. and Sutherland, A. (2023) Synthesis of Thiazoloindole α‑Amino Acids: Chromophores Amenable to One- and Two-Photon Induced Fluorescence. Organic Letters, 25(49), pp. 8942-8946. (doi: 10.1021/acs.orglett.3c03851) (PMID:38055619) (PMCID:PMC10729019)
Nobis, D., Sansom, H. G. and Magennis, S. W. (2023) Pulse-shaped broadband multiphoton excitation for single-molecule fluorescence detection in the far field. Methods and Applications in Fluorescence, 11(1), 017001. (doi: 10.1088/2050-6120/aca87f)
Bianco, S. , Hu, T., Henrich, O. and Magennis, S. W. (2022) Heterogeneous migration routes of DNA triplet repeat slip-outs. Biophysical Reports, 2(3), 100070. (doi: 10.1016/j.bpr.2022.100070) (PMID:36299495) (PMCID:PMC9586884)
Samaan, G. N., Wyllie, M. K., Cizmic, J. M., Needham, L.-M., Nobis, D., Ngo, K., Andersen, S., Magennis, S. W. , Lee, S. F. and Purse, B. W. (2021) Single-molecule fluorescence detection of a tricyclic nucleoside analogue. Chemical Science, 12(7), pp. 2623-2628. (doi: 10.1039/D0SC03903A) (PMID:34164030) (PMCID:PMC8179283)
Hu, T., Morten, M. J. and Magennis, S. M. (2021) Conformational and migrational dynamics of slipped-strand DNA three-way junctions containing trinucleotide repeats. Nature Communications, 12, 204. (doi: 10.1038/s41467-020-20426-3) (PMID:33420051) (PMCID:PMC7794359)
Morten, M. J., Steinmark, E. and Magennis, S. W. (2020) Probing DNA dynamics: stacking-induced fluorescence increase (SIFI) versus FRET. ChemPhotoChem, 4(9), pp. 664-667. (doi: 10.1002/cptc.202000069)
Berrocal-Martin, R., Sanchez-Cano, C., Chiu, C. K.C., Needham, R. J., Sadler, P. J. and Magennis, S. W. (2020) Metallation‐induced heterogeneous dynamics of DNA revealed by single‐molecule FRET. Chemistry: A European Journal, 26(22), pp. 4980-4987. (doi: 10.1002/chem.202000458) (PMID:31999015)
Berrones‐Reyes, J. C., Muñoz‐Flores, B. M., Uscanga‐Palomeque, A. C., Santillán, R., Del Angel‐Mosqueda, C., Nobis, D., Cochrane, M. A., Magennis, S. and Jiménez‐Pérez, V. M. (2020) Two‐photon detection of organotin Schiff base complexes in cancer cells. ChemistrySelect, 5(5), pp. 1623-1627. (doi: 10.1002/slct.201904816)
Bell, J. D., Harkiss, A. H., Nobis, D., Malcolm, E., Knuhtsen, A., Wellaway, C. R., Jamieson, A. G. , Magennis, S. W. and Sutherland, A. (2020) Conformationally rigid pyrazoloquinazoline α-amino acids: one- and two-photon induced fluorescence. Chemical Communications, 56(12), pp. 1887-1890. (doi: 10.1039/C9CC09064A) (PMID:31956866)
Nobis, D., Fisher, R. S., Simmermacher, M., Hopkins, P. A., Tor, Y., Jones, A. C. and Magennis, S. W. (2019) Single-molecule detection of a fluorescent nucleobase analog via multiphoton excitation. Journal of Physical Chemistry Letters, 10(17), pp. 5008-5012. (doi: 10.1021/acs.jpclett.9b02108) (PMID:31397575)
Morten, M. J., Lopez, S. G., Steinmark, I. E., Rafferty, A. and Magennis, S. W. (2018) Stacking-induced fluorescence increase reveals allosteric interactions through DNA. Nucleic Acids Research, 46(21), pp. 11618-11626. (doi: 10.1093/nar/gky887) (PMID:30277520) (PMCID:PMC6265455)
Bood, M. et al. (2018) Pentacyclic adenine: a versatile and exceptionally bright fluorescent DNA base analog. Chemical Science, 9(14), pp. 3494-3502. (doi: 10.1039/C7SC05448C) (PMID:29780479) (PMCID:PMC5934695)
Baltierra Jasso, L., Morten, M. and Magennis, S. W. (2018) Sub-ensemble monitoring of DNA strand displacement using multiparameter single-molecule FRET. ChemPhysChem, 19(5), pp. 551-555. (doi: 10.1002/cphc.201800012) (PMID:29316151)
Fisher, R. S., Nobis, D., Füchtbauer, A. F., Bood, M., Grøtli, M., Wilhelmsson, L. M., Jones, A. C. and Magennis, S. W. (2018) Pulse-shaped two-photon excitation of a fluorescent base analogue approaches single-molecule sensitivity. Physical Chemistry Chemical Physics, 20(45), pp. 28487-28498. (doi: 10.1039/c8cp05496g) (PMID:30412214)
Venkatesh, V. et al. (2017) Mitochondria-targeted spin-labelled luminescent iridium anticancer complexes. Chemical Science, 8(12), pp. 8271-8278. (doi: 10.1039/C7SC03216A)
Magennis, S. , Toulmin, A., Baltierra-Jasso, L. E., Morten, M. J., Sabir, T., McGlynn, P., Schröder, G. F., Smith, B. O. and Magennis, S. W. (2017) Conformational heterogeneity in a fully-complementary DNA three-way junction with a GC-rich branchpoint. Biochemistry, 56(37), pp. 4985-4991. (doi: 10.1021/acs.biochem.7b00677) (PMID:28820590)
Quinn, S. D. and Magennis, S. W. (2017) Optical detection of gadolinium(III) ions via quantum dot aggregation. RSC Advances, 7(40), pp. 24730-24735. (doi: 10.1039/C7RA03969G) (PMID:29308186) (PMCID:PMC5735353)
Dalton, C. E., Quinn, S. D. , Rafferty, A., Morten, M. J., Gardiner, J. M. and Magennis, S. W. (2016) Single-molecule fluorescence detection of a synthetic heparan sulfate disaccharide. ChemPhysChem, 17(21), pp. 3442-3446. (doi: 10.1002/cphc.201600750) (PMID:27538128) (PMCID:PMC5111599)
Quinn, S. D. , Rafferty, A., Dick, E., Morten, M. J., Kettles, F. J., Knox, C., Murrie, M. and Magennis, S. W. (2016) Surface charge control of quantum dot blinking. Journal of Physical Chemistry C, 120(34), pp. 19487-19491. (doi: 10.1021/acs.jpcc.6b07779)
Baltierra-Jasso, L. E., Morten, M., Laflör, L., Quinn, S. D. and Magennis, S. W. (2016) DNA hairpin dynamics under molecular crowding conditions. Biophysical Journal, 110(3 S1), 634a-635a. (doi: 10.1016/j.bpj.2015.11.3397)
Quinn, S. D. , Dalton, C. E., Jeanneret, R. A., Gardiner, J. M. and Magennis, S. W. (2016) Single-molecule studies of fluorescently-labelled polysaccharides. Biophysical Journal, 110(3 S1), 636a-636a.
Baltierra-Jasso, L. E., Morten, M. J., Laflor, L., Quinn, S. D. and Magennis, S. W. (2015) Crowding-induced hybridization of single DNA hairpins. Journal of the American Chemical Society, 137(51), pp. 16020-16023. (doi: 10.1021/jacs.5b11829) (PMID:26654490)
Lane, R. S. K., Jones, R., Sinkeldam, R. W., Tor, Y. and Magennis, S. W. (2014) Two-photon-induced fluorescence of isomorphic nucleobase analogs. ChemPhysChem, 15(5), pp. 867-871. (doi: 10.1002/cphc.201400031)
Pudney, C.R., Lane, R.S.K., Fielding, A.J., Magennis, S.W. , Hay, S. and Scrutton, N.S. (2013) Enzymatic single-molecule kinetic isotope effects. Journal of the American Chemical Society, 135(10), pp. 3855-3864. (doi: 10.1021/ja309286r)
Sabir, T., Toulmin, A., Ma, L., Jones, A.C., McGlynn, P., Schröder, G.F. and Magennis, S.W. (2012) Branchpoint expansion in a fully complementary three-way DNA junction. Journal of the American Chemical Society, 134(14), pp. 6280-6285. (doi: 10.1021/ja211802z)
Sabir, T., Schröder, G.F., Toulmin, A., McGlynn, P. and Magennis, S.W. (2011) Global structure of forked DNA in solution revealed by high-resolution single-molecule FRET. Journal of the American Chemical Society, 133(5), pp. 1188-1191. (doi: 10.1021/ja108626w)
Channon, K.J., Devlin, G.L., Magennis, S.W. , Finlayson, C.E., Tickler, A.K., Silva, C. and MacPhee, C.E. (2008) Modification of fluorophore photophysics through peptide-driven self-assembly. Journal of the American Chemical Society, 130(16), pp. 5487-5491. (doi: 10.1021/ja710310c)
Research datasets
2024
Cadden, G., Schloetel, J.-G., McKenzie, G., Boocock, M., Magennis, S. and Stark, M. (2024) Direct observation of subunit rotation during DNA strand exchange by serine recombinases. [Data Collection]
Cadden, G., Wilken, S. and Magennis, S. (2024) A single CAA interrupt in a DNA three-way junction containing a CAG repeat hairpin results in parity-dependent trapping. [Data Collection]
2022
Magennis, S. and Heinrich, O. (2022) Heterogeneous migration routes of DNA triplet repeat slip-outs. [Data Collection]
2020
Hu, T., Morten, M. J. and Magennis, S. (2020) Conformational and migrational dynamics of slipped-strand DNA three-way junctions containing trinucleotide repeats. [Data Collection]
2019
Nobis, D., Fisher, R. S., Simmermacher, M., Hopkins, P. A., Tor, Y., Jones, A. C. and Magennis, S. (2019) Single-molecule detection of a fluorescent nucleobase analog via multiphoton excitation. [Data Collection]
2018
Morten, M. J., Lopez, S., Steinmark, I. E., Rafferty, A. and Magennis, S. (2018) Stacking-induced fluorescence increase reveals allosteric interactions through DNA. [Data Collection]