Dr Craig Lygate

Reader in Myocardial Biology (Cardiovascular & Metabolic Health)

telephone: 01413303325
email: Craig.Lygate@glasgow.ac.uk
pronouns: He/him/his

Room 335, School of Cardiovascular Health, BHF GCRC, 126 University Place, Glasgow, G12 8TA

Biography

Craig Lygate originally trained as a pharmacist at the University of Strathclyde and the Royal Infirmary of Edinburgh, before obtaining a Masters of Research at the University of Manchester and a PhD in cardiovascular physiology at the University of Glasgow. He moved to Oxford University in 2000 to study cardiac energetics in the laboratory of Prof. Stefan Neubauer, where he established new cardiac phenotyping facilities and developed extensive expertise in the assessment of in vivo cardiac function and experimental models of hypertrophy, ischaemia and heart failure. From 2014-2024, he was Associate Professor of Cardiovascular Physiology in the Department of Cardiovascular Medicine, University of Oxford, where he ran a research group funded by the British Heart Foundation to study the interaction between creatine and homoarginine metabolism. In summer 2024, Craig moved his laboratory to the University of Glasgow, where he has been appointed Reader in Myocardial Biology.

Research interests

My research aims to understand the contribution of cellular energy metabolism to cardiac function in health and disease. In particular, to explore the therapeutic potential of augmenting cardiac energetics in the setting of acute ischaemia and chronic heart failure. In this context, the creatine kinase (CK) system is of special interest, since it acts as an energy buffer and transport system in the heart. We have studied the importance of a fully-functioning CK system using knockout models, e.g. hearts without creatine or CK. These hearts have a reduced ability to increase their workload at times of high energy demand and show impaired recovery following acute myocardial ischaemia. The reverse is also true, whereby augmentation of the CK system, e.g. by genetic overexpression of CK or the creatine transporter (SLC6A8), enhances functional recovery and reduces tissue damage in the ischaemic heart. Understanding the biochemical regulation of the creatine transporter is therefore a key focus of our current research alongside the identification of small molecule modulators, which we are pursuing in collaboration with AstraZeneca under their 'Open Innovation' platform.
Another key focus of my current research is the metabolite homoarginine, which shares a biosynthetic enzyme with creatine. There is no known biochemical requirement for homoarginine, yet low plasma homoarginine is associated with adverse outcomes in human populations. In preclinical studies, we have shown that low levels of homoarginine impairs the ability of the heart to contract and relax, while elevated levels help maintain contractile function in the setting of chronic heart failure. Understanding the mechanisms behind these observations are a major focus of my current research.
Most recently, I have been working with Dr Tanveer Tabish at the University of Oxford on a multi-disciplinary project to develop graphene-based nanoparticles for the therapeutic delivery of nitric oxide for use in cardiovascular applications. This has led to a UK patent application which describes a novel NO-generating coating for vascular stents to prevent restenosis and promote vascular healing.

Research groups

Publications

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Number of items: 105.

2024

Zervou, S., McAndrew, D. J., Lake, H. A., Kuznecova, E., Preece, C., Davies, B., Neubauer, S. and Lygate, C. A. (2024) Cardiac function and energetics in mice with combined genetic augmentation of creatine and creatine kinase activity. Journal of Molecular and Cellular Cardiology, 196, pp. 105-114. (doi: 10.1016/j.yjmcc.2024.09.007) (PMID:39276853)

Tabish, T. A. et al. (2024) Synthesis and characterization of amine-functionalized graphene as a nitric oxide-generating coating for vascular stents. Applied Physics Reviews, 11(3), 031422. (doi: 10.1063/5.0192379) (PMID:39355510) (PMCID:PMC7616654)

Tabish, T. A. et al. (2024) pH-sensitive release of nitric oxide gas using peptide-graphene co-assembled hybrid nanosheets. Nitric Oxide, 147, pp. 42-50. (doi: 10.1016/j.niox.2024.04.008) (PMID:38631610)

Tabish, T. A. et al. (2024) S-nitrosocysteamine-functionalised porous graphene oxide nanosheets as nitric oxide delivery vehicles for cardiovascular applications. Redox Biology, 72, 103144. (doi: 10.1016/j.redox.2024.103144) (PMID:38613920) (PMCID:PMC11026843)

Tabish, T. A., Crabtree, M. J., Townley, H. E., Winyard, P. G. and Lygate, C. A. (2024) Nitric oxide releasing nanomaterials for cardiovascular applications. JACC: Basic to Translational Science, 9(5), pp. 691-709. (doi: 10.1016/j.jacbts.2023.07.017) (PMID:38984042) (PMCID:PMC11228123)

Lygate, C. A. (2024) Maintaining energy provision in the heart: the creatine kinase system in ischaemia–reperfusion injury and chronic heart failure. Clinical Science, 138(8), pp. 491-514. (doi: 10.1042/CS20230616) (PMID:38639724) (PMCID:PMC11040329)

2023

Tabish, T. A., Zhu, Y., Shukla, S., Kadian, S., Sangha, G. S., Lygate, C. A. and Narayan, R. J. (2023) Graphene nanocomposites for real-time electrochemical sensing of nitric oxide in biological systems. Applied Physics Reviews, 10, 041310. (doi: 10.1063/5.0162640) (PMID:38229764) (PMCID:PMC7615530)

Farzi, M., Coveney, S., Afzali, M., Zdora, M.‐C., Lygate, C. A. , Rau, C., Frangi, A. F., Dall'Armellina, E., Teh, I. and Schneider, J. E. (2023) Measuring cardiomyocyte cellular characteristics in cardiac hypertrophy using diffusion‐weighted MRI. Magnetic Resonance in Medicine, 90(5), pp. 2144-2157. (doi: 10.1002/mrm.29775) (PMID:37345727) (PMCID:PMC10962572)

Maguire, M. L., McAndrew, D. J., Lake, H. A., Ostrowski, P. J., Zervou, S., Neubauer, S., Lygate, C. A. and Schneider, J. E. (2023) Synergistic effect on cardiac energetics by targeting the creatine kinase system: in vivo application of high-resolution 31P-CMRS in the mouse. Journal of Cardiovascular Magnetic Resonance, 25(1), p. 6. (doi: 10.1186/s12968-023-00911-6) (PMID:36740688) (PMCID:PMC9900916)

McAndrew, D. J., Lake, H. A., Zervou, S., Schwedhelm, E., Schneider, J. E., Neubauer, S. and Lygate, C. A. (2023) Homoarginine and creatine deficiency do not exacerbate murine ischaemic heart failure. ESC Heart Failure, 10(1), pp. 189-199. (doi: 10.1002/ehf2.14183) (PMID:36178450) (PMCID:PMC9871656)

Tabish, T. A. and Lygate, C. A. (2023) Mitochondria-targeted nanomedicines for cardiovascular applications. Nanomedicine, 18(28), pp. 2101-2104. (doi: 10.2217/nnm-2023-0321) (PMID:38059500)

2022

Monga, S., Valkovič, L., Tyler, D., Lygate, C. A. , Rider, O., Myerson, S. G., Neubauer, S. and Mahmod, M. (2022) Insights into the metabolic aspects of aortic stenosis with the use of magnetic resonance imaging. JACC: Cardiovascular Imaging, 15(12), pp. 2112-2126. (doi: 10.1016/j.jcmg.2022.04.025) (PMID:36481080) (PMCID:PMC9722407)

Nitz, K. et al. (2022) The amino acid homoarginine inhibits atherogenesis by modulating T-cell function. Circulation Research, 131(8), pp. 701-712. (doi: 10.1161/CIRCRESAHA.122.321094) (PMID:36102188)

Lygate, C. A. , Lake, H. A., McAndrew, D. J., Neubauer, S. and Zervou, S. (2022) Influence of homoarginine on creatine accumulation and biosynthesis in the mouse. Frontiers in Nutrition, 9, 969702. (doi: 10.3389/fnut.2022.969702) (PMID:36017222) (PMCID:PMC9395972)

2021

Farzi, M., Mcclymont, D., Whittington, H., Zdora, M.-C., Khazin, L., Lygate, C. A. , Rau, C., Dall'Armellina, E., Teh, I. and Schneider, J. E. (2021) Assessing myocardial microstructure with biophysical models of diffusion MRI. IEEE Transactions on Medical Imaging, 40(12), pp. 3775-3786. (doi: 10.1109/TMI.2021.3097907) (PMID:34270420)

Lygate, C. A. (2021) The pitfalls of in vivo cardiac physiology in genetically modified mice – lessons learnt the hard way in the creatine kinase system. Frontiers in Physiology, 12, 685064. (doi: 10.3389/fphys.2021.685064) (PMID:34054587) (PMCID:PMC8160301)

Zervou, S. et al. (2021) Subtle role for adenylate kinase 1 in maintaining normal basal contractile function and metabolism in the murine heart. Frontiers in Physiology, 12, 623969. (doi: 10.3389/fphys.2021.623969) (PMID:33867998) (PMCID:PMC8044416)

Carnicer, R. et al. (2021) BH4 increases nNOS activity and preserves left ventricular function in diabetes. Circulation Research, 128(5), pp. 585-601. (doi: 10.1161/CIRCRESAHA.120.316656) (PMID:33494625) (PMCID:PMC7612785)

Branovets, J., Karro, N., Barsunova, K., Laasmaa, M., Lygate, C. A. , Vendelin, M. and Birkedal, R. (2021) Cardiac expression and location of hexokinase changes in a mouse model of pure creatine deficiency. American Journal of Physiology: Heart and Circulatory Physiology, 320(2), H613-H629. (doi: 10.1152/AJPHEART.00188.2020)

Laasmaa, M., Branovets, J., Barsunova, K., Karro, N., Lygate, C. A. , Birkedal, R. and Vendelin, M. (2021) Altered calcium handling in cardiomyocytes from arginine-glycine amidinotransferase-knockout mice is rescued by creatine. American Journal of Physiology: Heart and Circulatory Physiology, 320(2), H805-H825. (doi: 10.1152/AJPHEART.00300.2020) (PMID:33275525)

2020

Peterzan, M. A. et al. (2020) Cardiac energetics in patients with aortic stenosis and preserved versus reduced ejection fraction. Circulation, 141(24), pp. 1971-1985. (doi: 10.1161/circulationaha.119.043450) (PMID:32438845) (PMCID:PMC7294745)

Aksentijević, D., Zervou, S., Eykyn, T. R., McAndrew, D. J., Wallis, J., Schneider, J. E., Neubauer, S. and Lygate, C. A. (2020) Age-dependent decline in cardiac function in Guanidinoacetate-N-methyltransferase knockout mice. Frontiers in Physiology, 10, 1535. (doi: 10.3389/fphys.2019.01535) (PMID:32038270) (PMCID:PMC6985570)

Cao, F., Maguire, M. L., McAndrew, D. J., Lake, H. A., Neubauer, S., Zervou, S., Schneider, J. E. and Lygate, C. A. (2020) Overexpression of mitochondrial creatine kinase preserves cardiac energetics without ameliorating murine chronic heart failure. Basic Research in Cardiology, 115(2), 12. (doi: 10.1007/s00395-020-0777-3) (PMID:31925563) (PMCID:PMC6954138)

2019

Valli, A. et al. (2019) Adaptation to HIF1α deletion in hypoxic cancer cells by upregulation of GLUT14 and creatine metabolism. Molecular Cancer Research, 17(7), pp. 1531-1544. (doi: 10.1158/1541-7786.MCR-18-0315) (PMID:30885992)

Clarke, W. T. et al. (2019) Localized rest and stress human cardiac creatine kinase reaction kinetics at 3 T. NMR in Biomedicine, 32(6), e4085. (doi: 10.1002/nbm.4085) (PMID:30920054) (PMCID:PMC6542687)

2018

Cao, F., Zervou, S. and Lygate, C. A. (2018) The creatine kinase system as a therapeutic target for myocardial ischaemia–reperfusion injury. Biochemical Society Transactions, 46(5), pp. 1119-1127. (doi: 10.1042/BST20170504) (PMID:30242115) (PMCID:PMC6195634)

Stockebrand, M. et al. (2018) A mouse model of creatine transporter deficiency reveals impaired motor function and muscle energy metabolism. Frontiers in Physiology, 9, 773. (doi: 10.3389/fphys.2018.00773) (PMID:30013483) (PMCID:PMC6036259)

Whittington, H. J. et al. (2018) Over-expression of mitochondrial creatine kinase in the murine heart improves functional recovery and protects against injury following ischaemia–reperfusion. Cardiovascular Research, 114(6), pp. 858-869. (doi: 10.1093/cvr/cvy054) (PMID:29509881) (PMCID:PMC5909653)

Faller, K. M. E. et al. (2018) Impaired cardiac contractile function in arginine:glycine amidinotransferase knockout mice devoid of creatine is rescued by homoarginine but not creatine. Cardiovascular Research, 114(3), pp. 417-430. (doi: 10.1093/cvr/cvx242) (PMID:29236952) (PMCID:PMC5982714)

Chuaiphichai, S., Rashbrook, V. S., Hale, A. B., Trelfa, L., Patel, J., McNeill, E., Lygate, C. A. , Channon, K. M. and Douglas, G. (2018) Endothelial cell tetrahydrobiopterin modulates sensitivity to ang (angiotensin) II–induced vascular remodeling, blood pressure, and abdominal aortic aneurysm. Hypertension, 72(1), pp. 128-138. (doi: 10.1161/HYPERTENSIONAHA.118.11144) (PMID:29844152) (PMCID:PMC6012043)

2017

Peterzan, M. A., Lygate, C. A. , Neubauer, S. and Rider, O. J. (2017) Metabolic remodeling in hypertrophied and failing myocardium: a review. American Journal of Physiology: Heart and Circulatory Physiology, 313(3), H597-H616. (doi: 10.1152/ajpheart.00731.2016)

Gallyas, F., Zervou, S., Whittington, H. J., Ostrowski, P. J., Cao, F., Tyler, J., Lake, H. A., Neubauer, S. and Lygate, C. A. (2017) Increasing creatine kinase activity protects against hypoxia / reoxygenation injury but not against anthracycline toxicity in vitro. PLoS ONE, 12(8), e0182994. (doi: 10.1371/journal.pone.0182994) (PMID:28806770) (PMCID:PMC5555628)

Lygate, C. A. (2017) Metabolic arithmetic: do two wrongs make a right? Cardiovascular Research, 113(10), pp. 1093-1095. (doi: 10.1093/cvr/cvx121) (PMID:28641382)

Carnicer, R., Suffredini, S., Liu, X., Reilly, S., Simon, J. N., Surdo, N. C., Zhang, Y. H., Lygate, C. A. , Channon, K. M. and Casadei, B. (2017) The subcellular localization of neuronal nitric oxide synthase determines the downstream effects of NO on myocardial function. Cardiovascular Research, 113(3), pp. 321-331. (doi: 10.1093/cvr/cvx002) (PMID:28158509) (PMCID:PMC5408949)

Atzler, D., McAndrew, D. J., Cordts, K., Schneider, J. E., Zervou, S., Schwedhelm, E., Neubauer, S. and Lygate, C. A. (2017) Dietary supplementation with homoarginine preserves cardiac function in a murine model of post-myocardial infarction heart failure. Circulation, 135(4), pp. 400-402. (doi: 10.1161/CIRCULATIONAHA.116.025673) (PMID:28115416)

Smart, N. et al. (2017) Aberrant developmental titin splicing and dysregulated sarcomere length in Thymosin β4 knockout mice. Journal of Molecular and Cellular Cardiology, 102, pp. 94-107. (doi: 10.1016/j.yjmcc.2016.10.010) (PMID:27914791) (PMCID:PMC5319848)

2016

Teh, I., McClymont, D., Zdora, M.-C., Whittington, H. J., Davidoiu, V., Lee, J., Lygate, C. A. , Rau, C., Zanette, I. and Schneider, J. E. (2016) Validation of diffusion tensor MRI measurements of cardiac microstructure with structure tensor synchrotron radiation imaging. Journal of Cardiovascular Magnetic Resonance, 19(1), 31. (doi: 10.1186/s12968-017-0342-x) (PMID:28279178) (PMCID:PMC5345150)

Zak, J. et al. (2016) ASPP2 deficiency causes features of 1q41q42 microdeletion syndrome. Cell Death and Differentiation, 23(12), pp. 1973-1984. (doi: 10.1038/cdd.2016.76) (PMID:27447114) (PMCID:PMC5136487)

Teh, I., McClymont, D., Burton, R. A. B., Maguire, M. L., Whittington, H. J., Lygate, C. A. , Kohl, P. and Schneider, J. E. (2016) Resolving fine cardiac structures in rats with high-resolution diffusion tensor imaging. Scientific Reports, 6, 30573. (doi: 10.1038/srep30573) (PMID:27466029) (PMCID:PMC4964346)

Bertrand, L., Whittington, H. J., McAndrew, D. J., Cross, R. L., Neubauer, S. and Lygate, C. A. (2016) Protective effect of creatine elevation against ischaemia reperfusion injury is retained in the presence of co-morbidities and during cardioplegia. PLoS ONE, 11(1), e0146429. (doi: 10.1371/journal.pone.0146429) (PMID:26765737) (PMCID:PMC4713158)

Lygate, C. A. (2016) Energetics in the hypertrophied and failing heart. In: Schwarzer, M. and Doenst, T. (eds.) The Scientist's Guide to Cardiac Metabolism. Elsevier (Academic Press), pp. 183-190. ISBN 9780128023945 (doi: 10.1016/B978-0-12-802394-5.00013-3)

Zervou, S., Whittington, H. J., Russell, A. J. and Lygate, C. A. (2016) Augmentation of creatine in the heart. Mini-Reviews in Medicinal Chemistry, 16(1), pp. 19-28. (doi: 10.2174/1389557515666150722102151) (PMID:26202199) (PMCID:PMC4634222)

Zervou, S. et al. (2016) Proteomic and metabolomic changes driven by elevating myocardial creatine suggest novel metabolic feedback mechanisms. Amino Acids, 48(8), pp. 1969-1981. (doi: 10.1007/s00726-016-2236-x) (PMID:27143170) (PMCID:PMC4974297)

2015

Ruparelia, N. et al. (2015) Acute myocardial infarction activates distinct inflammation and proliferation pathways in circulating monocytes, prior to recruitment, and identified through conserved transcriptional responses in mice and humans. European Heart Journal, 36(29), pp. 1923-1934. (doi: 10.1093/eurheartj/ehv195) (PMID:25982896) (PMCID:PMC4571177)

Douglas, G., Hale, A. B., Crabtree, M. J., Ryan, B. J., Hansler, A., Watschinger, K., Gross, S. S., Lygate, C. A. , Alp, N. J. and Channon, K. M. (2015) A requirement for Gch1 and tetrahydrobiopterin in embryonic development. Developmental Biology, 399, pp. 129-138. (doi: 10.1016/j.ydbio.2014.12.025) (PMID:25557619) (PMCID:PMC4347993)

Faller, K. M.E., McAndrew, D. J., Schneider, J. E. and Lygate, C. A. (2015) Refinement of analgesia following thoracotomy and experimental myocardial infarction using the Mouse Grimace Scale. Experimental Physiology, 100(2), pp. 164-172. (doi: 10.1113/expphysiol.2014.083139) (PMID:25480160) (PMCID:PMC4340041)

Maguire, M. L., Geethanath, S., Lygate, C. A. , Kodibagkar, V. D. and Schneider, J. E. (2015) Compressed sensing to accelerate magnetic resonance spectroscopic imaging: evaluation and application to 23Na-imaging of mouse hearts. Journal of Cardiovascular Magnetic Resonance, 17(1), 45. (doi: 10.1186/s12968-015-0149-6) (PMID:26073300) (PMCID:PMC4466859)

2014

Zazueta, C., Aksentijević, D., Zervou, S., Faller, K. M.E., McAndrew, D. J., Schneider, J. E., Neubauer, S. and Lygate, C. A. (2014) Myocardial creatine levels do not influence response to acute oxidative stress in isolated perfused heart. PLoS ONE, 9(10), e109021. (doi: 10.1371/journal.pone.0109021) (PMID:25272153) (PMCID:PMC4182806)

Aksentijević, D. et al. (2014) Cardiac dysfunction and peri-weaning mortality in malonyl-coenzyme A decarboxylase (MCD) knockout mice as a consequence of restricting substrate plasticity. Journal of Molecular and Cellular Cardiology, 75, pp. 76-87. (doi: 10.1016/j.yjmcc.2014.07.008) (PMID:25066696) (PMCID:PMC4169183)

Lygate, C. A. and Neubauer, S. (2014) The myocardial creatine kinase system in the normal, ischaemic and failing heart. In: Lopaschuk, G. D. and Dhalla, N. S. (eds.) Cardiac Energy Metabolism in Health and Disease. Series: Advances in biochemistry in health and disease (11). Springer: New York, NY, pp. 155-168. (doi: 10.1007/978-1-4939-1227-8_10)

Masoud, W. G.T., Ussher, J. R., Wang, W., Jaswal, J. S., Wagg, C. S., Dyck, J. R., Lygate, C. A. , Neubauer, S., Clanachan, A. S. and Lopaschuk, G. D. (2014) Failing mouse hearts utilize energy inefficiently and benefit from improved coupling of glycolysis and glucose oxidation. Cardiovascular Research, 101(1), pp. 30-38. (doi: 10.1093/cvr/cvt216) (PMID:24048945)

2013

Faller, K. M.E., Lygate, C. A. , Neubauer, S. and Schneider, J. E. (2013) 1H-MR spectroscopy for analysis of cardiac lipid and creatine metabolism. Heart Failure Reviews, 18(5), pp. 657-668. (doi: 10.1007/s10741-012-9341-z) (PMID:22945240) (PMCID:PMC3782658)

Zervou, S., Ray, T., Sahgal, N., Sebag-Montefiore, L., Cross, R., Medway, D. J., Ostrowski, P. J., Neubauer, S. and Lygate, C. A. (2013) A role for thioredoxin-interacting protein (Txnip) in cellular creatine homeostasis. American Journal of Physiology: Endocrinology and Metabolism, 305(2), E263-E270. (doi: 10.1152/ajpendo.00637.2012) (PMID:23715727) (PMCID:PMC3725544)

Faller, K. M.E., Medway, D. J., Aksentijevic, D., Sebag-Montefiore, L., Schneider, J. E., Lygate, C. A. and Neubauer, S. (2013) Ribose supplementation alone or with elevated creatine does not preserve high energy nucleotides or cardiac function in the failing mouse heart. PLoS ONE, 8(6), e66461. (doi: 10.1371/journal.pone.0066461) (PMID:23823183) (PMCID:PMC3688916)

Branovets, J., Sepp, M., Kotlyarova, S., Jepihhina, N., Sokolova, N., Aksentijevic, D., Lygate, C. A. , Neubauer, S., Vendelin, M. and Birkedal, R. (2013) Unchanged mitochondrial organization and compartmentation of high-energy phosphates in creatine-deficient GAMT−/−mouse hearts. American Journal of Physiology: Heart and Circulatory Physiology, 305(4), H506-H520. (doi: 10.1152/ajpheart.00919.2012) (PMID:23792673) (PMCID:PMC3891243)

Ruparelia, N., Digby, J. E., Jefferson, A., Medway, D. J., Neubauer, S., Lygate, C. A. and Choudhury, R. P. (2013) Myocardial infarction causes inflammation and leukocyte recruitment at remote sites in the myocardium and in the renal glomerulus. Inflammation Research, 62(5), pp. 515-525. (doi: 10.1007/s00011-013-0605-4) (PMID:23471223) (PMCID:PMC3625409)

Lygate, C. A. , Schneider, J. E. and Neubauer, S. (2013) Investigating cardiac energetics in heart failure. Experimental Physiology, 98(3), pp. 601-605. (doi: 10.1113/expphysiol.2012.064709) (PMID:22983996)

Lygate, C. A. et al. (2013) Living without creatine: unchanged exercise capacity and response to chronic myocardial infarction in creatine-deficient mice. Circulation Research, 112(6), pp. 945-955. (doi: 10.1161/CIRCRESAHA.112.300725) (PMID:23325497) (PMCID:PMC4182017)

Lygate, C. A. and Neubauer, S. (2013) Metabolic flux as a predictor of heart failure prognosis. Circulation Research, 114(8), pp. 1228-1230. (doi: 10.1161/circresaha.114.303551) (PMID:24723654) (PMCID:PMC4556065)

2012

Lygate, C. A. et al. (2012) Moderate elevation of intracellular creatine by targeting the creatine transporter protects mice from acute myocardial infarction. Cardiovascular Research, 96(3), pp. 466-475. (doi: 10.1093/cvr/cvs272) (PMID:22915766) (PMCID:PMC3500046)

Lygate, C. , Medway, D. J., Ostrowski, P. J., Aksentijevic, D., Sebag-Montefiore, L., Hunyor, I., Zervou, S., Schneider, J. E. and Neubauer, S. (2012) Chronic creatine kinase deficiency eventually leads to congestive heart failure, but severity is dependent on genetic background, gender and age. Basic Research in Cardiology, 107(5), 276. (doi: 10.1007/s00395-012-0276-2) (PMID:22760499) (PMCID:PMC3442167)

Ashrafian, H. et al. (2012) Fumarate is cardioprotective via activation of the Nrf2 antioxidant pathway. Cell Metabolism, 15(3), pp. 361-371. (doi: 10.1016/j.cmet.2012.01.017) (PMID:22405071) (PMCID:PMC3314920)

Wech, T., Lygate, C. A. , Neubauer, S., Köstler, H. and Schneider, J. E. (2012) Highly accelerated cardiac functional MRI in rodent hearts using compressed sensing and parallel imaging at 9.4T. Journal of Cardiovascular Magnetic Resonance, 14(S1), P65. (doi: 10.1186/1532-429X-14-S1-P65) (PMCID:PMC3305770)

Wech, T., Medway, D., Lygate, C. A. , Neubauer, S., Köstler, H. and Schneider, J. E. (2012) Accurate infarct-size measurements from accelerated, compressed sensing reconstructed cine-MRI images in mouse hearts. Journal of Cardiovascular Magnetic Resonance, 14(S1), P57. (doi: 10.1186/1532-429X-14-S1-P57) (PMCID:PMC3305705)

Dall'Armellina, E., Jung, B. A., Lygate, C. A. , Neubauer, S., Markl, M. and Schneider, J. E. (2012) Improved method for quantification of regional cardiac function in mice using phase‐contrast MRI. Magnetic Resonance in Medicine, 67(2), pp. 541-551. (doi: 10.1002/mrm.23022) (PMID:21674616) (PMCID:PMC3378699)

2011

Wech, T., Lemke, A., Medway, D., Stork, L.‐A., Lygate, C. A. , Neubauer, S., Köstler, H. and Schneider, J. E. (2011) Accelerating cine‐MR imaging in mouse hearts using compressed sensing. Journal of Magnetic Resonance Imaging, 34(5), pp. 1072-1079. (doi: 10.1002/jmri.22718) (PMID:21932360) (PMCID:PMC3261377)

Young, A. A., Medway, D. J., Lygate, C. A. , Neubauer, S. and Schneider, J. E. (2011) Accelerating global left-ventricular function assessment in mice using reduced slice acquisition and three-dimensional guide-point modelling. Journal of Cardiovascular Magnetic Resonance, 13, 49. (doi: 10.1186/1532-429X-13-49) (PMID:21917165) (PMCID:PMC3182947)

Schneider, J. E., Lanz, T., Barnes, H., Stork, L.‐A., Bohl, S., Lygate, C. A. , Ordidge, R. J. and Neubauer, S. (2011) Accelerated cardiac magnetic resonance imaging in the mouse using an eight‐channel array at 9.4 Tesla. Magnetic Resonance in Medicine, 65(1), pp. 60-70. (doi: 10.1002/mrm.22605) (PMID:20740650) (PMCID:PMC3021721)

2010

Aksentijević, D., Lygate, C. A. , Makinen, K., Zervou, S., Sebag‐Montefiore, L., Medway, D., Barnes, H., Schneider, J. E. and Neubauer, S. (2010) High‐energy phosphotransfer in the failing mouse heart: role of adenylate kinase and glycolytic enzymes. European Journal of Heart Failure, 12(12), pp. 1282-1289. (doi: 10.1093/eurjhf/hfq174) (PMID:20940173) (PMCID:PMC2990411)

Horwitz, M. S. et al. (2010) A mutation in the mitochondrial fission gene Dnm1l leads to cardiomyopathy. PLoS Genetics, 6(6), e1001000. (doi: 10.1371/journal.pgen.1001000) (PMID:20585624) (PMCID:PMC2891719)

Fischer, A., ten Hove, M., Sebag-Montefiore, L., Wagner, H., Clarke, K., Watkins, H., Lygate, C. A. and Neubauer, S. (2010) Changes in creatine transporter function during cardiac maturation in the rat. BMC Developmental Biology, 10, 70. (doi: 10.1186/1471-213X-10-70) (PMID:20569423) (PMCID:PMC2909979)

Bohl, S., Lygate, C. A. , Schulz-Menger, J., Neubauer, S. and Schneider, J. E. (2010) T2-mapping of ischaemia/reperfusion-injury in the in vivo mouse heart. Journal of Cardiovascular Magnetic Resonance, 12(S1), O61. (doi: 10.1186/1532-429X-12-S1-O61)

2009

Bohl, S., Medway, D. J., Schulz-Menger, J., Schneider, J. E., Neubauer, S. and Lygate, C. A. (2009) Refined approach for quantification of in vivo ischemia-reperfusion injury in the mouse heart. American Journal of Physiology: Heart and Circulatory Physiology, 297(6), H2054-H2058. (doi: 10.1152/ajpheart.00836.2009) (PMID:19820193) (PMCID:PMC2793132)

Phillips, D. et al. (2009) Mice over-expressing the myocardial creatine transporter develop progressive heart failure and show decreased glycolytic capacity. Journal of Molecular and Cellular Cardiology, 48(4), pp. 582-590. (doi: 10.1016/j.yjmcc.2009.10.033) (PMID:19913546) (PMCID:PMC2837781)

Bohl, S., Lygate, C. A. , Barnes, H., Medway, D., Stork, L.-A., Schulz-Menger, J., Neubauer, S. and Schneider, J. E. (2009) Advanced methods for quantification of infarct size in mice using three-dimensional high-field late gadolinium enhancement MRI. American Journal of Physiology: Heart and Circulatory Physiology, 296(4), H1200-H1208. (doi: 10.1152/ajpheart.01294.2008) (PMID:19218501) (PMCID:PMC2670705)

2008

Lygate, C. A. , Hunyor, I., Medway, D., de Bono, J. P., Dawson, D., Wallis, J., Sebag-Montefiore, L. and Neubauer, S. (2008) Cardiac phenotype of mitochondrial creatine kinase knockout mice is modified on a pure C57BL/6 genetic background. Journal of Molecular and Cellular Cardiology, 46(1), pp. 93-99. (doi: 10.1016/j.yjmcc.2008.09.710) (PMID:18948110)

Hove, M. t., Makinen, K., Sebag-Montefiore, L., Hunyor, I., Fischer, A., Wallis, J., Isbrandt, D., Lygate, C. and Neubauer, S. (2008) Creatine uptake in mouse hearts with genetically altered creatine levels. Journal of Molecular and Cellular Cardiology, 45(3), pp. 453-459. (doi: 10.1016/j.yjmcc.2008.05.023) (PMID:18602925) (PMCID:PMC2568826)

Murray, A. J., Cole, M. A., Lygate, C. A. , Carr, C. A., Stuckey, D. J., Little, S. E., Neubauer, S. and Clarke, K. (2008) Increased mitochondrial uncoupling proteins, respiratory uncoupling and decreased efficiency in the chronically infarcted rat heart. Journal of Molecular and Cellular Cardiology, 44(4), pp. 694-700. (doi: 10.1016/j.yjmcc.2008.01.008) (PMID:18328500)

Schneider, J. E., Lanz, T., Barnes, H., Medway, D., Stork, L.‐A., Lygate, C. A. , Smart, S., Griswold, M. A. and Neubauer, S. (2008) Ultra‐fast and accurate assessment of cardiac function in rats using accelerated MRI at 9.4 Tesla. Magnetic Resonance in Medicine, 59(3), pp. 636-641. (doi: 10.1002/mrm.21491) (PMID:18306411)

Bishop, T. et al. (2008) Abnormal sympathoadrenal development and systemic hypotension in PHD3−/− mice. Molecular and Cellular Biology, 28(10), pp. 3386-3400. (doi: 10.1128/MCB.02041-07) (PMID:18332118) (PMCID:PMC2423159)

Schneider, J. E., Stork, L.-A., Bell, J. T., Hove, M. t., Isbrandt, D., Clarke, K., Watkins, H., Lygate, C. A. and Neubauer, S. (2008) Cardiac structure and function during ageing in energetically compromised Guanidinoacetate N-methyltransferase (GAMT)-knockout mice – a one year longitudinal MRI study. Journal of Cardiovascular Magnetic Resonance, 10(1), p. 9. (doi: 10.1186/1532-429x-10-9) (PMID:18275592) (PMCID:PMC2254407)

2007

Lygate, C. A. , Fischer, A., Sebag-Montefiore, L., Wallis, J., ten Hove, M. and Neubauer, S. (2007) The creatine kinase energy transport system in the failing mouse heart. Journal of Molecular and Cellular Cardiology, 42(6), pp. 1129-1136. (doi: 10.1016/j.yjmcc.2007.03.899) (PMID:17481652)

Lygate, C. (2007) Letter to the editor: Infarct size measurements are critically important when comparing interventions affecting ventricular remodeling. American Journal of Physiology: Heart and Circulatory Physiology, 293(5), H3221-H3221. (doi: 10.1152/ajpheart.00908.2007)

2006

Heather, L., Cole, M., Lygate, C. , Evans, R., Stuckey, D., Murray, A., Neubauer, S. and Clarke, K. (2006) Fatty acid transporter levels and palmitate oxidation rate correlate with ejection fraction in the infarcted rat heart. Cardiovascular Research, 72(3), pp. 430-437. (doi: 10.1016/j.cardiores.2006.08.020) (PMID:17034771)

Murray, A., Lygate, C. , Cole, M., Carr, C., Radda, G., Neubauer, S. and Clarke, K. (2006) Insulin resistance, abnormal energy metabolism and increased ischemic damage in the chronically infarcted rat heart. Cardiovascular Research, 71(1), pp. 149-157. (doi: 10.1016/j.cardiores.2006.02.031) (PMID:16616054)

Willam, C., Maxwell, P. H., Nichols, L., Lygate, C. , Tian, Y. M., Bernhardt, W., Wiesener, M., Ratcliffe, P. J., Eckardt, K.-U. and Pugh, C. W. (2006) HIF prolyl hydroxylases in the rat; organ distribution and changes in expression following hypoxia and coronary artery ligation. Journal of Molecular and Cellular Cardiology, 41(1), pp. 68-77. (doi: 10.1016/j.yjmcc.2006.04.009) (PMID:16765982)

Wilding, J. R., Lygate, C. A. , Davies, K. E., Neubauer, S. and Clarke, K. (2006) MLP accumulation and remodelling in the infarcted rat heart. European Journal of Heart Failure, 8(4), pp. 343-346. (doi: 10.1016/j.ejheart.2005.10.006) (PMID:16330255)

Lygate, C. (2006) Pharmacists should be more vocal on the benefits of animal research. Pharmaceutical Journal, 276,

Davies, J. K. et al. (2006) Characterization of the role of γ2 R531G mutation in AMP-activated protein kinase in cardiac hypertrophy and Wolff-Parkinson-White syndrome. American Journal of Physiology: Heart and Circulatory Physiology, 290(5), H1942-H1951. (doi: 10.1152/ajpheart.01020.2005)

Lygate, C. A. , Schneider, J. E., Hulbert, K., Hove, M., Sebag-Montefiore, L. M., Cassidy, P. J., Clarke, K. and Neubauer, S. (2006) Serial high resolution 3D–MRI after aortic banding in mice: band internalization is a source of variability in the hypertrophic response. Basic Research in Cardiology, 101(1), pp. 8-16. (doi: 10.1007/s00395-005-0546-3) (PMID:16132171)

Lygate, C. (2006) Surgical models of hypertrophy and heart failure: Myocardial infarction and transverse aortic constriction. Drug Discovery Today: Disease Models, 3(3), pp. 283-290. (doi: 10.1016/j.ddmod.2006.10.002)

Schneider, J., Wiesmann, F., Lygate, C. and Neubauer, S. (2006) How to perform an accurate assessment of cardiac function in mice using high-resolution magnetic resonance imaging. Journal of Cardiovascular Magnetic Resonance, 8(5), pp. 693-701. (doi: 10.1080/10976640600723664) (PMID:16891228)

Tyler, D., Lygate, C. , Schneider, J., Cassidy, P., Neubauer, S. and Clarke, K. (2006) CINE-MR imaging of the normal and infarcted rat heart using an 11.7 T vertical bore MR system. Journal of Cardiovascular Magnetic Resonance, 8(2), pp. 327-333. (doi: 10.1080/10976640500451903) (PMID:16669175) (PMCID:16669175)

2005

Tenhove, M., Chan, S., Lygate, C. , Monfared, M., Boehm, E., Hulbert, K., Watkins, H., Clarke, K. and Neubauer, S. (2005) Mechanisms of creatine depletion in chronically failing rat heart. Journal of Molecular and Cellular Cardiology, 38(2), pp. 309-313. (doi: 10.1016/j.yjmcc.2004.11.016) (PMID:15698837)

Dawson, D., Lygate, C. A. , Zhang, M.-H., Hulbert, K., Neubauer, S. and Casadei, B. (2005) nNOS gene deletion exacerbates pathological left ventricular remodeling and functional deterioration after myocardial infarction. Circulation, 112(24), pp. 3729-3737. (doi: 10.1161/CIRCULATIONAHA.105.539437) (PMID:16344403)

ten Hove, M. et al. (2005) Reduced inotropic reserve and increased susceptibility to cardiac ischemia/reperfusion injury in phosphocreatine-deficient guanidinoacetate- N -methyltransferase–knockout mice. Circulation, 111(19), pp. 2477-2485. (doi: 10.1161/01.CIR.0000165147.99592.01) (PMID:15883212)

Wallis, J. et al. (2005) Supranormal myocardial creatine and phosphocreatine concentrations lead to cardiac hypertrophy and heart failure. Circulation, 112(20), pp. 3131-3139. (doi: 10.1161/CIRCULATIONAHA.105.572990) (PMID:16286605)

2004

Cassidy, P. J., Schneider, J. E., Grieve, S. M., Lygate, C. , Neubauer, S. and Clarke, K. (2004) Assessment of motion gating strategies for mouse magnetic resonance at high magnetic fields. Journal of Magnetic Resonance Imaging, 19(2), pp. 229-237. (doi: 10.1002/jmri.10454) (PMID:14745758)

Dawson, D., Lygate, C. A. , Saunders, J., Schneider, J. E., Ye, X., Hulbert, K., Noble, J. A. and Neubauer, S. (2004) Quantitative 3-dimensional echocardiography for accurate and rapid cardiac phenotype characterization in mice. Circulation, 110(12), pp. 1632-1637. (doi: 10.1161/01.CIR.0000142049.14227.AD) (PMID:15364813)

Schäfer, A., Alp, N. J., Cai, S., Lygate, C. A. , Neubauer, S., Eigenthaler, M., Bauersachs, J. and Channon, K. M. (2004) Reduced vascular NO bioavailability in diabetes increases platelet activation in vivo. Arteriosclerosis, Thrombosis, and Vascular Biology, 24(9), pp. 1720-1726. (doi: 10.1161/01.ATV.0000138072.76902.dd) (PMID:15242858)

Schneider, J. E., Hulbert, K. J., Lygate, C. A. , Hove, M. T., Cassidy, P. J., Clarke, K. and Neubauer, S. (2004) Long-term stability of cardiac function in normal and chronically failing mouse hearts in a vertical-bore MR system. Magnetic Resonance Materials in Physics, Biology and Medicine, 17(3-6), pp. 162-169. (doi: 10.1007/s10334-004-0056-6) (PMID:15538660)

2003

Lygate, C. A. , Hulbert, K., Monfared, M., Cole, M. A., Clarke, K. and Neubauer, S. (2003) The PPARγ-activator rosiglitazone does not alter remodeling but increases mortality in rats post-myocardial infarction. Cardiovascular Research, 58(3), pp. 632-637. (doi: 10.1016/S0008-6363(03)00289-X) (PMID:12798436)

Schneider, J. E., Cassidy, P. J., Lygate, C. , Tyler, D. J., Wiesmann, F., Grieve, S. M., Hulbert, K., Clarke, K. and Neubauer, S. (2003) Fast, high‐resolution in vivo cine magnetic resonance imaging in normal and failing mouse hearts on a vertical 11.7 T system. Journal of Magnetic Resonance Imaging, 18(6), pp. 691-701. (doi: 10.1002/jmri.10411) (PMID:14635154)

Sears, C. E., Bryant, S. M., Ashley, E. A., Lygate, C. A. , Rakovic, S., Wallis, H. L., Neubauer, S., Terrar, D. A. and Casadei, B. (2003) Cardiac neuronal nitric oxide synthase isoform regulates myocardial contraction and calcium handling. Circulation Research, 92(5), e52-9. (doi: 10.1161/01.RES.0000064585.95749.6D) (PMID:12623875)

2002

Brosnan, M. J., Hamilton, C. A., Graham, D., Lygate, C. , Jardine, E. and Dominiczak, A. F. (2002) Irbesartan lowers superoxide levels and increases nitric oxide bioavailability in blood vessels from spontaneously hypertensive stroke-prone rats. Journal of Hypertension, 20(2), pp. 281-286. (doi: 10.1097/00004872-200202000-00018) (PMID:11821713)

This list was generated on Wed Nov 20 20:28:48 2024 GMT.

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