Professor Laura Machesky
- Affiliate (School of Cancer Sciences)
email:
Laura.Machesky@glasgow.ac.uk
Department of Biochemistryry, 80 Tennis Court Road, Sanger Building, Cambridge, CB2 1GA
https://orcid.org/0000-0002-7592-9856Research interests
Link to Migration, Invasion and Metastasis website
Publications
2024
Nikolaou, S., Juin, A., Whitelaw, J. A., Paul, N. R., Fort, L., Nixon, C., Spence, H. J., Bryson, S. and Machesky, L. M. (2024) CYRI-B-mediated macropinocytosis drives metastasis via lysophosphatidic acid receptor uptake. eLife, 13, e83712. (doi: 10.7554/elife.83712) (PMID:38712822) (PMCID:PMC11219039)
Kapitany, V., Fatima, A., Zickus, V., Whitelaw, J., McGhee, E., Insall, R. , Machesky, L. and Faccio, D. (2024) Single-sample image-fusion upsampling of fluorescence lifetime images. Science Advances, 10(21), eadn0139. (doi: 10.1126/sciadv.adn0139)
Juin, A., Spence, H. J. and Machesky, L. M. (2024) Dichotomous role of the serine/threonine kinase MAP4K4 in pancreatic ductal adenocarcinoma onset and metastasis through control of AKT and ERK pathways. Journal of Pathology, 262(4), pp. 454-466. (doi: 10.1002/path.6248) (PMID:38229581)
2023
Morris, H. T. , Bamlet, W. R., Razidlo, G. L. and Machesky, L. M. (2023) FSCN1 and epithelial mesenchymal transformation transcription factor expression in human pancreatic intraepithelial neoplasia and ductal adenocarcinoma. Pathology: Research and Practice, 251, 154836. (doi: 10.1016/j.prp.2023.154836) (PMID:37832352) (PMCID:37832352)
Machesky, L. M. (2023) CYRI proteins: controllers of actin dynamics in the cellular ‘eat vs walk’ decision. Biochemical Society Transactions, 51(2), pp. 579-585. (doi: 10.1042/bst20221354) (PMID:36892409)
Wilkinson, E. L. et al. (2023) Genetically engineered multicistronic allele of Pmel yielding highly specific CreERT2-mediated recombination in the melanocyte lineage. Pigment Cell and Melanoma Research, 36(1), pp. 71-77. (doi: 10.1111/pcmr.13076) (PMID:36412082)
2022
Papalazarou, V., Drew, J., Juin, A., Spence, H. J., Whitelaw, J., Nixon, C., Salmeron-Sanchez, M. and Machesky, L. M. (2022) Collagen VI expression is negatively mechanosensitive in pancreatic cancer cells and supports the metastatic niche. Journal of Cell Science, 135(24), jcs259978. (doi: 10.1242/jcs.259978) (PMID:36546396) (PMCID:PMC9845737)
2021
Le, A. H., Yelland, T., Paul, N. R., Fort, L., Nikolaou, S., Ismail, S. and Machesky, L. M. (2021) CYRI-A limits invasive migration through macropinosome formation and integrin uptake regulation. Journal of Cell Biology, 220(9), e202012114. (doi: 10.1083/jcb.202012114) (PMID:34165494) (PMCID:PMC8236918)
Olson, M. F. and Machesky, L. M. (2021) MICAL2 fine-tunes Arp2/3 for actin branching. Journal of Cell Biology, 220(8), e202106176. (doi: 10.1083/jcb.202106176) (PMID:34264264)
Drew, J. and Machesky, L. M. (2021) The liver metastatic niche: modelling the extracellular matrix in metastasis. Disease Models and Mechanisms, 14(4), dmm048801. (doi: 10.1242/dmm.048801) (PMID:33973625) (PMCID:PMC8077555)
Yelland, T., Le, A. H., Nikolaou, S., Insall, R. , Machesky, L. and Ismail, S. (2021) Structural basis of CYRI-B direct competition with Scar/WAVE complex for Rac1. Structure, 29(3), 226-237.e4. (doi: 10.1016/j.str.2020.11.003) (PMID:33217330) (PMCID:PMC7955166)
Swaminathan, K., Campbell, A., Papalazarou, V., Jaber-Hijazi, F. , Nixon, C., McGhee, E., Strathdee, D., Sansom, O. J. and Machesky, L. M. (2021) The RAC1 target NCKAP1 plays a crucial role in the progression of Braf;Pten-driven melanoma in mice. Journal of Investigative Dermatology, 141(3), 628-637.e15. (doi: 10.1016/j.jid.2020.06.029) (PMID:32777214)
Callenberg, C., Lyons, A. , den Brok, D., Fatima, A., Turpin, A. , Zickus, V., Machesky, L. , Whitelaw, J., Faccio, D. and Hullin, M.B. (2021) Super-resolution time-resolved imaging using computational sensor fusion. Scientific Reports, 11, 1689. (doi: 10.1038/s41598-021-81159-x) (PMID:33462284) (PMCID:PMC7813875)
Pickering, K.A. et al. (2021) A RAC-GEF network critical for early intestinal tumourigenesis. Nature Communications, 12, 56. (doi: 10.1038/s41467-020-20255-4) (PMID:33397922) (PMCID:PMC7782582)
2020
Zickus, V. et al. (2020) Fluorescence lifetime imaging with a megapixel SPAD camera and neural network lifetime estimation. Scientific Reports, 10, 20986. (doi: 10.1038/s41598-020-77737-0) (PMID:33268900) (PMCID:PMC7710711)
Papalazarou, V., Swaminathan, K., Jaber-Hijazi, F. , Spence, H., Lahmann, I., Nixon, C., Salmeron-Sanchez, M. , Arnold, H.-H., Rottner, K. and Machesky, L. M. (2020) The Arp2/3 complex is crucial for colonisation of the mouse skin by melanoblasts. Development, 147(22), dev194555. (doi: 10.1242/dev.194555) (PMID:33028610) (PMCID:PMC7687863)
Tweedy, L., Thomason, P. A., Paschke, P. I., Martin, K., Machesky, L. M. , Zagnoni, M. and Insall, R. H. (2020) Seeing around corners: cells solve mazes and respond at a distance using attractant breakdown. Science, 369(6507), eaay9792. (doi: 10.1126/science.aay9792) (PMID:32855311)
Singh, S. P. , Thomason, P. A., Lilla, S., Schaks, M., Tang, Q., Goode, B. L., Machesky, L. M. , Rottner, K. and Insall, R. H. (2020) Cell–substrate adhesion drives Scar/WAVE activation and phosphorylation by a Ste20-family kinase, which controls pseudopod lifetime. PLoS Biology, 18(8), e3000774. (doi: 10.1371/journal.pbio.3000774) (PMID:32745097) (PMCID:PMC7425996)
McMenamin, P. G., Shields, G. T., Seyed-Razavi, Y., Kalirai, H., Insall, R. H. , Machesky, L. M. and Coupland, S. E. (2020) Melanoblasts populate the mouse choroid earlier in development than previously described. Investigative Ophthalmology and Visual Science, 61(10), 33. (doi: 10.1167/iovs.61.10.33) (PMID:32797202) (PMCID:PMC7441366)
Whitelaw, J. A., Swaminathan, K., Kage, F. and Machesky, L. M. (2020) The WAVE regulatory complex is required to balance protrusion and adhesion in migration. Cells, 9(7), 1635. (doi: 10.3390/cells9071635) (PMID:32646006) (PMCID:PMC7407199)
Nikolaou, S. and Machesky, L. (2020) The stressful tumour environment drives plasticity of cell migration programmes, contributing to metastasis. Journal of Pathology, 250(5), pp. 612-623. (doi: 10.1002/path.5395) (PMID:32057095) (PMCID:PMC7216910)
Papalazarou, V., Zhang, T., Paul, N. R., Juin, A., Cantini, M. , Maddocks, O. D.K. , Salmeron-Sanchez, M. and Machesky, L. (2020) The creatine-phosphagen system is mechanoresponsive in pancreatic adenocarcinoma and fuels invasion and metastasis. Nature Metabolism, 2(1), pp. 62-80. (doi: 10.1038/s42255-019-0159-z) (PMID:32694686)
2019
Schurr, Y. et al. (2019) Platelet lamellipodium formation is not required for thrombus formation and stability. Blood, 134(25), pp. 2318-2329. (doi: 10.1182/blood.2019002105) (PMID:31697813)
Amato, C., Thomason, P. A., Davidson, A. J., Swaminathan, K., Ismail, S. , Machesky, L. M. and Insall, R. H. (2019) WASP restricts active Rac to maintain cells’ front-rear polarization. Current Biology, 29(24), pp. 4169-4182. (doi: 10.1016/j.cub.2019.10.036) (PMID:31786060) (PMCID:PMC6926487)
Juin, A. et al. (2019) N-WASP control of LPAR1 trafficking establishes response to self-generated LPA gradients to promote pancreatic cancer cell metastasis. Developmental Cell, 51(4), 431-445.e7. (doi: 10.1016/j.devcel.2019.09.018) (PMID:31668663) (PMCID:PMC6863394)
Francis, S. et al. (2019) Structure-based design, synthesis and biological evaluation of a novel series of isoquinolone and pyrazolo[4,3-c]pyridine inhibitors of fascin 1 as potential anti-metastatic agents. Bioorganic and Medicinal Chemistry Letters, 29(8), pp. 1023-1029. (doi: 10.1016/j.bmcl.2019.01.035) (PMID:30773430) (PMCID:PMC6419574)
Nikolaou, S. and Machesky, L. M. (2019) Invasion by force: The C. elegans anchor cell leads the way. Developmental Cell, 48(3), pp. 287-288. (doi: 10.1016/j.devcel.2019.01.024)
Kugeratski, F. G. et al. (2019) Hypoxic cancer–associated fibroblasts increase NCBP2-AS2/HIAR to promote endothelial sprouting through enhanced VEGF signaling. Science Signaling, 12(567), eaan8247. (doi: 10.1126/scisignal.aan8247) (PMID:30723174) (PMCID:PMC6794160)
Machesky, L. M. (2019) Rab11FIP proteins link endocytic recycling vesicles for cytoskeletal transport and tethering. Bioscience Reports, 39(1), BSR2018221. (doi: 10.1042/bsr20182219) (PMID:30622149) (PMCID:PMC6356010)
Bachg, A. C. et al. (2019) Phenotypic analysis of Myo10 knockout (Myo10tm2/tm2) mice lacking full-length (motorized) but not brain-specific headless myosin X. Scientific Reports, 9, 597. (doi: 10.1038/s41598-018-37160-y) (PMID:30679680) (PMCID:PMC6345916)
Siebert, S. , Insall, R. and Machesky, L. M. (2019) More means less: managing overflow in science publishing. In: Czarniawska, Barbara and Löfgren, Orvar (eds.) Overwhelmed by Overflows? How People and Organizations Create and Manage Excess. Lund University Press: Lund, pp. 130-150. ISBN 9789198469813 (doi: 10.7765/9789198469813.00013)
2018
Papalazarou, V., Salmeron-Sanchez, M. and Machesky, L. M. (2018) Tissue engineering the cancer microenvironment—challenges and opportunities. Biophysical Reviews, 10(6), pp. 1695-1711. (doi: 10.1007/s12551-018-0466-8) (PMID:30406572) (PMCID:PMC6297082)
Fort, L. et al. (2018) Fam49/CYRI interacts with Rac1 and locally suppresses protrusions. Nature Cell Biology, 20(10), pp. 1159-1171. (doi: 10.1038/s41556-018-0198-9) (PMID:30250061) (PMCID:PMC6863750)
Morris, H. T. , Fort, L., Spence, H. J., Patel, R., Vincent, D. F., Park, J. H. , Snapper, S. B., Carey, F. A., Sansom, O. J. and Machesky, L. M. (2018) Loss of N-WASP drives early progression in an Apc model of intestinal tumourigenesis. Journal of Pathology, 245(3), pp. 337-348. (doi: 10.1002/path.5086) (PMID:29672847) (PMCID:PMC6033012)
Laurent, P., Petit, V., Aktary, Z., Gallagher, S., Tweedy, L., Machesky, L. and Larue, L. (2018) Simulation of melanoblast displacements reveals new features of developmental migration. Development, 145(12), dev160200. (doi: 10.1242/dev.160200) (PMID:29769218) (PMCID:PMC6031402)
2017
Machesky, L. and Braga, V. M.M. (2017) So far, yet so close: α-Catenin dimers help migrating cells get together. Journal of Cell Biology, 216(11), pp. 3437-3439. (doi: 10.1083/jcb.201709056) (PMID:29051263)
Giampazolias, E. et al. (2017) Mitochondrial permeabilization engages NF-κB-dependent anti-tumour activity under caspase deficiency. Nature Cell Biology, 19(9), pp. 1116-1129. (doi: 10.1038/ncb3596) (PMID:28846096) (PMCID:PMC5624512)
Reid, S. E. et al. (2017) Tumor matrix stiffness promotes metastatic cancer cell interaction with the endothelium. EMBO Journal, 36(16), pp. 2373-2389. (doi: 10.15252/embj.201694912) (PMID:28694244) (PMCID:PMC5556271)
Woodham, E. F. et al. (2017) Coordination by Cdc42 of actin, contractility, and adhesion for melanoblast movement in mouse skin. Current Biology, 27(5), pp. 624-637. (doi: 10.1016/j.cub.2017.01.033) (PMID:28238662) (PMCID:PMC5344686)
Thomas, S. G., Poulter, N. S., Bem, D., Finney, B., Machesky, L. M. and Watson, S. P. (2017) The actin binding proteins cortactin and HS1 are dispensable for platelet actin nodule and megakaryocyte podosome formation. Platelets, 28(4), pp. 372-379. (doi: 10.1080/09537104.2016.1235688) (PMID:27778524) (PMCID:PMC5274539)
2016
Tyrrell, B. J., Woodham, E. F., Spence, H. J., Strathdee, D., Insall, R. H. and Machesky, L. M. (2016) Loss of strumpellin in the melanocytic lineage impairs the WASH Complex but does not affect coat colour. Pigment Cell and Melanoma Research, 29(5), pp. 559-571. (doi: 10.1111/pcmr.12506) (PMID:27390154) (PMCID:PMC5082549)
Sinclair, A. et al. (2016) CXCR2 and CXCL4 regulate survival and self-renewal of hematopoietic stem/progenitor cells. Blood, 128(3), pp. 371-383. (doi: 10.1182/blood-2015-08-661785) (PMID:27222476) (PMCID:PMC4991087)
García, E., Ragazzini, C., Yu, X., Cuesta-García, E., Bernardino de la Serna, J., Zech, T., Sarrió, D., Machesky, L. M. and Antón, I. M. (2016) WIP and WICH/WIRE co-ordinately control invadopodium formation and maturation in human breast cancer cell invasion. Scientific Reports, 6, 23590. (doi: 10.1038/srep23590) (PMID:27009365) (PMCID:PMC4806363)
2015
Ma, Y. and Machesky, L. (2015) Fascin1 in carcinomas: Its regulation and prognostic value. International Journal of Cancer, 137(11), pp. 2534-2544. (doi: 10.1002/ijc.29260) (PMID:25302416)
Siebert, S. , Machesky, L. M. and Insall, R. H. (2015) Overflow in science and its implications for trust. eLife, 4, e10825. (doi: 10.7554/eLife.10825)
Giancotti, F. G., Haynes, C. M., Machesky, L. M. , Olson, M. F. and Overholtzer, M. (2015) Alan Hall 1952–2015. Nature Cell Biology, 17(7), pp. 839-840. (doi: 10.1038/ncb3190) (PMID:26005836)
Rainero, E., Howe, J. D., Caswell, P. T., Jamieson, N. B. , Anderson, K., Critchley, D. R., Machesky, L. and Norman, J. C. (2015) Ligand-occupied integrin internalization links nutrient signaling to invasive migration. Cell Reports, 10(3), pp. 398-413. (doi: 10.1016/j.celrep.2014.12.037)
Vijayakumar, V., Monypenny, J., Chen, X. J., Machesky, L. M. , Lilla, S., Thrasher, A. J., Anton, I. M., Calle, Y. and Jones, G. E. (2015) Tyrosine phosphorylation of WIP releases bound WASP and impairs podosome assembly in macrophages. Journal of Cell Science, 128(2), pp. 251-265. (doi: 10.1242/jcs.154880)
Lindsay, C. R., Li, A., Faller, W., Ozanne, B., Welch, H., Machesky, L. M. and Sansom, O. (2015) A Rac1-independent role for P-Rex1 in melanoblasts. Journal of Investigative Dermatology, 135(1), pp. 314-318. (doi: 10.1038/jid.2014.323)
Ma, Y., Faller, W., Sansom, O. , Brown, E. R., Doig, T. N., Melton, D. W. and Machesky, L. (2015) Fascin expression is increased in metastatic lesions but does not correlate with progression nor outcome in melanoma. Melanoma Research, 25(2), pp. 169-172. (doi: 10.1097/CMR.0000000000000135) (PMID:25535872)
Morris, H.T. and Machesky, L.M. (2015) Actin cytoskeletal control during epithelial to mesenchymal transition: focus on the pancreas and intestinal tract. British Journal of Cancer, 112(4), pp. 613-620. (doi: 10.1038/bjc.2014.658) (PMID:25611303) (PMCID:PMC4333498)
2014
Muinonen-Martin, A. J. et al. (2014) Melanoma cells break down LPA to establish local gradients that drive chemotactic dispersal. PLoS Biology, 12(10), e1001966. (doi: 10.1371/journal.pbio.1001966) (PMID:25313567) (PMCID:PMC4196730)
García, E., Machesky, L. M. , Jones, G. E. and Antón, I. M. (2014) WIP is necessary for matrix invasion by breast cancer cells. European Journal of Cell Biology, 93(10-12), pp. 413-423. (doi: 10.1016/j.ejcb.2014.07.008)
Woodham, E. F. and Machesky, L. M. (2014) Polarised cell migration: intrinsic and extrinsic drivers. Current Opinion in Cell Biology, 30, pp. 25-32. (doi: 10.1016/j.ceb.2014.05.006)
Machesky, L. and Sedwick, C. (2014) Laura Machesky: actin opens the way. Journal of Cell Biology, 206(7), pp. 816-817. (doi: 10.1083/jcb.2067pi) (PMID:25267291) (PMCID:PMC4178962)
Li, A. et al. (2014) Fascin is regulated by slug, promotes progression of pancreatic cancer in mice, and is associated with patient outcomes. Gastroenterology, 146(5), 1386-1396.e17. (doi: 10.1053/j.gastro.2014.01.046) (PMID:24462734) (PMCID:PMC4000441)
Tyrrell, B. J., Neilson, M., Insall, R. H. and Machesky, L. M. (2014) Predicting cell shapes in melanomas. Pigment Cell and Melanoma Research, 27(1), pp. 5-6. (doi: 10.1111/pcmr.12176) (PMID:24118871)
2013
Schachtner, H., Li, A., Stevenson, D., Calaminus, S. D.J., Thomas, S. G., Watson, S. P., Sixt, M., Wedlich-Soldner, R., Strathdee, D. and Machesky, L. M. (2013) Tissue inducible Lifeact expression allows visualization of actin dynamics in vivo and ex vivo. European Journal of Cell Biology, 91(11-12), pp. 923-929. (doi: 10.1016/j.ejcb.2012.04.002)
Futó, K., Bódis, E., Machesky, L.M. , Nyitrai, M. and Visegrády, B. (2013) Membrane binding properties of IRSp53-missing in metastasis domain (IMD) protein. Biochimica et Biophysica Acta: Molecular and Cell Biology of Lipids, 1831(11), pp. 1651-1655. (doi: 10.1016/j.bbalip.2013.07.006)
Ma, Y., Reynolds, L. E., Li, A., Stevenson, R. P., Hodivala-Dilke, K. M., Yamashiro, S. and Machesky, L. M. (2013) Fascin 1 is dispensable for developmental and tumour angiogenesis. Biology Open, 2(11), pp. 1187-1191. (doi: 10.1242/bio.20136031) (PMID:24244855) (PMCID:PMC3828765)
Schachtner, H., Calaminus, S. D. J., Thomas, S. G. and Machesky, L. M. (2013) Podosomes in adhesion, migration, mechanosensing and matrix remodeling. Cytoskeleton, 70(10), pp. 572-589. (doi: 10.1002/cm.21119)
Ampe, C., Machesky, L.M. and Nyitrai, M. (2013) Biophysical approaches for investigation of the cytoskeleton. A special issue stemming from research presented at the 2012 ECF meeting: A FEBS/EMBO workshop lecture course in Pécs, Hungary. Cytoskeleton, 70(10), p. 539. (doi: 10.1002/cm.21145)
Sonego, M., Gajendra, S., Parsons, M., Ma, Y., Hobbs, C., Zentar, M.P., Williams, G., Machesky, L.M. , Doherty, P. and Lalli, G. (2013) Fascin regulates the migration of subventricular zone-derived neuroblasts in the postnatal brain. Journal of Neuroscience, 33(30), pp. 12171-12185. (doi: 10.1523/JNEUROSCI.0653-13.2013)
Ma, Y., Li, A., Faller, W.J., Libertini, S., Fiorito, F., Gillespie, D.A., Sansom, O.J. , Yamashiro, S. and Machesky, L.M. (2013) Fascin 1 is transiently expressed in mouse melanoblasts during development and promotes migration and proliferation. Development, 140(10), pp. 2203-2211. (doi: 10.1242/dev.089789)
Li, A. and Machesky, L. M. (2013) Rac1 cycling fast in melanoma with P29S. Pigment Cell and Melanoma Research, 26(3), pp. 289-290. (doi: 10.1111/pcmr.12074) (PMID:23530970)
Schachtner, H. et al. (2013) Megakaryocytes assemble podosomes that degrade matrix and protrude through basement membrane. Blood, 121(13), pp. 2542-2552. (doi: 10.1182/blood-2012-07-443457) (PMID:23305739)
Zanivan, S. et al. (2013) In Vivo SILAC-based proteomics reveals phosphoproteome changes during mouse skin carcinogenesis. Cell Reports, 3(2), pp. 552-566. (doi: 10.1016/j.celrep.2013.01.003)
Park, L., Thomason, P.A., Zech, T., King, J.S., Veltman, D.M., Carnell, M., Ura, S., Machesky, L.M. and Insall, R.H. (2013) Cyclical Action of the WASH complex: FAM21 and capping protein drive WASH recycling, not initial recruitment. Developmental Cell, 24(2), pp. 169-181. (doi: 10.1016/j.devcel.2012.12.014)
Tang, H. et al. (2013) Loss of scar/WAVE complex promotes N-WASP- and FAK-dependent invasion. Current Biology, 23(2), pp. 107-117. (doi: 10.1016/j.cub.2012.11.059)
2012
Calaminus, S. D.J., Guitart, A. V., Sinclair, A., Schachtner, H., Watson, S. P., Holyoake, T. L., Kranc, K. R. and Machesky, L. M. (2012) Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny. PLoS ONE, 7(12), e51361. (doi: 10.1371/journal.pone.0051361) (PMID:23300543) (PMCID:PMC3531453)
Davidson, A.J., Veltman, D.M., Machesky, L.M. and Insall, R.H. (2012) The Interplay Between SCAR (WAVE) and WASP in Actin-Based Motility. 2012 American Society for Cell Biology Annual Meeting, San Francisco, CA, USA, 15-19 Dec 2012. p. 1823. (doi: 10.1091/mbc.e12-10-0757)
Monteiro, P., Hertzog, M., Rossé, C., Desnos, C., Zech, T., Formstecher, E., Darchen, F., Machesky, L. , Gautreau, A. and Chavrier, P. (2012) Co-ordinated Functions of WASH and Exocyst Complex Underlie the Biogenesis of Invadopodia in Metastatic Breast Tumor Cells. 2012 American Society for Cell Biology Annual Meeting, San Francisco, CA, USA, 15-19 Dec 2012. p. 2077. (doi: 10.1091/mbc.e12-10-0757)
Zech, T. et al. (2012) N-WASP Coordinates the Delivery and F-actin Mediated Reversible Capture of MT1-MMP at Invasive Pseudopods to Drive Breast Cancer Cell Invasion. 2012 American Society for Cell Biology Annual Meeting, San Francisco, CA, USA, 15-19 Dec 2012. p. 2076. (doi: 10.1091/mbc.e12-10-0757)
Machesky, L.M. and Sansom, O.J. (2012) Rac1 in the driver’s seat for melanoma. Pigment Cell and Melanoma Research, 25(6), pp. 762-764. (doi: 10.1111/pcmr.12004)
Yu, X. et al. (2012) N-WASP coordinates the delivery and F-actin-mediated capture of MT1-MMP at invasive pseudopods. Journal of Cell Biology, 199(3), pp. 527-544. (doi: 10.1083/jcb.201203025)
Veltman, D. M., King, J. S., Machesky, L. M. and Insall, R. H. (2012) SCAR knockouts in Dictyostelium: WASP assumes SCAR’s position and upstream regulators in pseudopods. Journal of Cell Biology, 198(4), pp. 501-508. (doi: 10.1083/jcb.201205058) (PMID:22891261) (PMCID:PMC3514037)
Maritzen, T., Zech, T., Schmidt, M.R., Krause, E., Machesky, L.M. and Haucke, V. (2012) Gadkin negatively regulates cell spreading and motility via sequestration of the actin-nucleating ARP2/3 complex. Proceedings of the National Academy of Sciences of the United States of America, 109(26), pp. 10382-10387. (doi: 10.1073/pnas.1206468109)
Stevenson, R. P., Myant, K., Jamieson, T., Ma, Y., Li, A., Tan, E. H., Yamashiro, S., Pignatelli, M., Sansom, O. and Machesky, L. M. (2012) Targeting the Actin Cytoskeletal Protein Fascin as a Novel Modulator of Intestinal Epithelial Cell Proliferation and Colon Cancer. Gastroenterology, 142(5 Sup1), S-44. (doi: 10.1016/S0016-5085(12)60167-X)
Ura, S., Pollitt, A.Y., Veltman, D.M., Morrice, N.A., Machesky, L.M. and Insall, R.H. (2012) Pseudopod growth and evolution during cell movement is controlled through SCAR/WAVE dephosphorylation. Current Biology, 22(7), pp. 553-561. (doi: 10.1016/j.cub.2012.02.020)
Doyle, B., Tan, E.H., Timpson, P., Machesky, L.M. , Ridgway, R.R., Jeffery, R.R., Poulsom, R., Morton, J.P. and Sansom, O.J. (2012) The Tumor Suppressor ARF Can Promote Invasion in the Absence of p53 Activity. United States and Canadian Academy of Pathology 101st Annual Meeting, Vancouver, BC, Canada, 17-23 Mar 2012. 461A. (doi: 10.1038/modpathol.2012.27)
Dawson, J.C., Bruche, S., Spence, H.J., Braga, V.M.M. and Machesky, L.M. (2012) Mtss1 promotes cell-cell junction assembly and stability through the small GTPase Rac1. PLoS ONE, 7(3), e31141. (doi: 10.1371/journal.pone.0031141) (PMID:22479308) (PMCID:PMC3313965)
Dawson, J.C., Timpson, P., Kalna, G. and Machesky, L.M. (2012) Mtss1 regulates epidermal growth factor signaling in head and neck squamous carcinoma cells. Oncogene, 31(14), pp. 1781-1793. (doi: 10.1038/onc.2011.376)
Futo, K., Bodis, E., Machesky, L.M. , Nyitrai, M. and Visegrady, B. (2012) Actin controls the I-BAR-membrane interaction. Molecular Biology of the Cell, 23,
García, E., Jones, G.E., Machesky, L.M. and Antón, I.M. (2012) WIP: WASP-interacting proteins at invadopodia and podosomes. European Journal of Cell Biology, 91(11-12), pp. 869-877. (doi: 10.1016/j.ejcb.2012.06.002)
Li, A., Ma, Y., Jin, M., Mason, S., Mort, R.L., Blyth, K. , Larue, L., Sansom, O.J. and Machesky, L.M. (2012) Activated mutant NRasQ61K drives aberrant melanocyte signaling, survival, and invasiveness via a rac1-Dependent mechanism. Journal of Investigative Dermatology, 132(11), pp. 2610-2621. (doi: 10.1038/jid.2012.186)
Li, A. and Machesky, L. M. (2012) Melanoblasts on the move: Rac1 sets the pace. Small GTPases, 3(12), pp. 115-119. (doi: 10.4161/sgtp.19494) (PMID:22790200)
Spence, H.J., Timpson, P., Tang, H.R., Insall, R.H. and Machesky, L.M. (2012) Scar/WAVE3 contributes to motility and plasticity of lamellipodial dynamics but not invasion in three dimensions. Biochemical Journal, 448(1), pp. 35-42. (doi: 10.1042/BJ20112206)
Stevenson, R.P., Veltman, D. and Machesky, L.M. (2012) Actin-bundling proteins in cancer progression at a glance. Journal of Cell Science, 125(5), pp. 1073-1079. (doi: 10.1242/jcs.093799)
Yu, X. and Machesky, L.M. (2012) Cells assemble invadopodia-like structures and invade into matrigel in a matrix metalloprotease dependent manner in the circular invasion assay. PLoS ONE, 7(2), e30605. (doi: 10.1371/journal.pone.0030605) (PMID:22347388) (PMCID:PMC3275555)
Zech, T., Calaminus, S. and Machesky, L. (2012) Actin on trafficking: could actin guide directed receptor transport? Cell Adhesion and Migration, 6(6), pp. 476-481. (doi: 10.4161/cam.21373) (PMID:23076144) (PMCID:PMC3547890)
2011
Li, A. et al. (2011) Rac1 drives melanoblast organization during mouse development by orchestrating pseudopod- driven motility and cell-cycle progression. Developmental Cell, 21(4), pp. 722-734. (doi: 10.1016/j.devcel.2011.07.008)
Futó, K., Machesky, L. M. and Visegrády, B. (2011) The Role of the I-BAR Domain in the Filopodia Formation. 8th EBSA European Biophysics Congress, Budapest, Hungary, 23-27 Aug 2011. S197. (doi: 10.1007/s00249-011-0734-z)
Thomas, S.G., Calaminus, S.D.J., Machesky, L.M. , Alberts, A.S. and Watson, S.P. (2011) G-protein coupled and ITAM receptor regulation of the formin FHOD1 through Rho Kinase in platelets. Journal of Thrombosis and Haemostasis, 9(8), pp. 1648-1651. (doi: 10.1111/j.1538-7836.2011.04357.x)
Machesky, L. (2011) Going on a (membrane) bender to the F-BAR. Developmental Cell, 20(1), e3. (doi: 10.1016/j.devcel.2010.11.023)
Carnell, M., Zech, T., Calaminus, S.D., Ura, S., Hagedorn, M., Johnston, S.A., May, R.C., Soldati, T., Machesky, L.M. and Insall, R.H. (2011) Actin polymerization driven by WASH causes V-ATPase retrieval and vesicle neutralization before exocytosis. Journal of Cell Biology, 193(5), pp. 831-839. (doi: 10.1083/jcb.201009119)
Lindsay, C.C.R. et al. (2011) P-Rex1 is required for efficient melanoblast migration and melanoma metastasis. Nature Communications, 2, pp. 1-9. (doi: 10.1038/ncomms1560)
Ramsay, A.K. et al. (2011) ERK5 signalling in prostate cancer promotes an invasive phenotype. British Journal of Cancer, 104(4), pp. 664-672. (doi: 10.1038/sj.bjc.6606062)
Vartiainen, M., Heath, R.J.W., Leong, J.M., Visegrády, B., Machesky, L.M. and Xavier, R.J. (2011) Bacterial and host determinants of MAL activation upon EPEC infection: the roles of Tir, ABRA, and FLRT3. PLoS Pathogens, 7(4), e1001332. (doi: 10.1371/journal.ppat.1001332)
Veltman, D.W., Auciello, G., Spence, H.J., Machesky, L.M. , Rappoport, J.Z. and Insall, R.H. (2011) Functional analysis of Dictyostelium IBARa reveals a conserved role of the I-BAR domain in endocytosis. Biochemical Journal, 436(1), pp. 45-52. (doi: 10.1042/BJ20101684)
Zech, T., Calaminus, S.D.J., Caswell, P., Spence, H.J., Carnell, M., Insall, R.H. , Norman, J. and Machesky, L.M. (2011) The Arp2/3 activator WASH regulates 5 1-integrin-mediated invasive migration. Journal of Cell Science, 124(22), pp. 3753-3759. (doi: 10.1242/jcs.080986)
2010
Calaminus, S. D., Inman, G. , Ghaevert, C., Sansom, O. , Watson, S. P., Holyoake, T. L. and Machesky, L. (2010) Alterations In Wnt Signalling In the Megakaryocytic Lineage Leads to Bone Marrow Failure and Myelofibrosis. Blood, 116(21), 628. (doi: 10.1182/blood.V116.21.628.628)
Serrels, B., Sandilands, E., Serrels, A., Baillie, G. , Houslay, M. D., Brunton, V. G., Canel, M., Machesky, L. M. , Anderson, K. I. and Frame, M. C. (2010) A complex between FAK, RACK1, and PDE4D5 controls spreading initiation and cancer cell polarity. Current Biology, 20(12), pp. 1086-1092. (doi: 10.1016/j.cub.2010.04.042)
Li, A., Dawson, J. C., Forero-Vargas, M., Spence, H. J., Yu, X., König, I., Anderson, K. and Machesky, L. M. (2010) The Actin-Bundling Protein Fascin Stabilizes Actin in Invadopodia and Potentiates Protrusive Invasion. Current Biology, 20(4), pp. 339-345. (doi: 10.1016/j.cub.2009.12.035) (PMID:20137952) (PMCID:PMC3163294)
Brooks, S. P., Coccia, M., Tang, H. R., Kanuga, N., Machesky, L. M. , Bailly, M., Cheetham, M. E. and Hardcastle, A. J. (2010) The Nance-Horan syndrome protein encodes a functional WAVE homology domain (WHD) and is important for co-ordinating actin remodelling and maintaining cell morphology. Human Molecular Genetics, 19(12), pp. 2421-2432. (doi: 10.1093/hmg/ddq125)
Machesky, L. M. and Li, A. (2010) Fascin: Invasive filopodia promoting metastasis. Communicative and Integrative Biology, 3(3), pp. 263-270. (doi: 10.4161/cib.3.3.11556) (PMID:20714410) (PMCID:PMC2918773)
Scott, R.W. et al. (2010) LIM kinases are required for invasive path generation by tumor and tumor-associated stromal cells. Journal of Cell Biology, 191(1), pp. 169-185. (doi: 10.1083/jcb.201002041)
Stein, T. et al. (2010) Loss of Reelin Expression in Breast Cancer Is Epigenetically Controlled and Associated with Poor Prognosis. American Journal of Pathology, 177(5), pp. 2323-2333. (doi: 10.2353/ajpath.2010.100209)
Visegrády, B. and Machesky, L.M. (2010) Myopathy-causing actin mutations promote defects in serum-response factor signalling. Biochemical Journal, 427(1), pp. 41-48. (doi: 10.1042/BJ20091641)
2009
Mardakheh, F. K., Yekezare, M., Machesky, L. M. and Heath, J. K. (2009) Spred2 interaction with the late endosomal protein NBR1 down-regulates fibroblast growth factor receptor signaling. Journal of Cell Biology, 187(2), pp. 265-277. (doi: 10.1083/jcb.200905118) (PMID:19822672) (PMCID:PMC2768835)
Vandamme, D., Lambert, E., Waterschoot, D., Tondeleir, D., Vandekerckhove, J., Machesky, L. M. , Constantin, B., Rommelaere, H. and Ampe, C. (2009) Phenotypes induced by NM causing α-skeletal muscle actin mutants in fibroblasts, Sol 8 myoblasts and myotubes. BMC Research Notes, 2, 40. (doi: 10.1186/1756-0500-2-40) (PMID:19284548) (PMCID:PMC2657152)
Insall, R. and Machesky, L.M. (2009) Actin Dynamics at the Leading Edge: From Simple Machinery to Complex Networks. Developmental Cell, 17(3), pp. 310-322. (doi: 10.1016/j.devcel.2009.08.012)
Machesky, L.M. and Tang, H.R. (2009) Actin-Based Protrusions: Promoters or Inhibitors of Cancer Invasion? Cancer Cell, 16(1), pp. 5-7. (doi: 10.1016/j.ccr.2009.06.009)
2008
Mardakheh, F.K., Yekezare, M., Machesky, L.M. and Heath, J.K. (2008) SPRED redirects activated receptors to lysosomes via scaffolding protein NBR1. European Journal of Cancer Supplements, 6(9), pp. 38-39. (doi: 10.1016/S1359-6349(08)71324-7)
Calaminus, S.D.J., Thomas, S., McCarty, O. J. T., Machesky, L.M. and Watson, S. P. (2008) Identification of a novel, actin-rich structure, the actin nodule, in the early stages of platelet spreading. Journal of Thrombosis and Haemostasis, 6(11), pp. 1944-1952. (doi: 10.1111/j.1538-7836.2008.03141.x)
Heung, M., Visegrady, B., Futterer, K. and Machesky, L. (2008) Identification of the insulin-responsive tyrosine phosphorylation sites on IRSp53. European Journal of Cell Biology, 87(8-9), pp. 699-708. (doi: 10.1016/j.ejcb.2008.02.010)
Johnston, S.A., Bramble, J.P., Yeung, C.L., Mendes, P.M. and Machesky, L.M. (2008) Arp2/3 complex activity in filopodia of spreading cells. BMC Cell Biology, 9(1), p. 65. (doi: 10.1186/1471-2121-9-65)
Machesky, L. (2008) Lamellipodia and filopodia in metastasis and invasion. FEBS Letters, 582(14), pp. 2102-2111. (doi: 10.1016/j.febslet.2008.03.039)
Machesky, L. , Jurdic, P. and Hinz, B. (2008) Grab, stick, pull and digest: the functional diversity of actin-associated matrix-adhesion structures. Workshop on Invadopodia, Podosomes and Focal Adhesions in Tissue Invasion. EMBO Reports, 9(2), pp. 139-143. (doi: 10.1038/sj.embor.7401162)
Zech, T. and Machesky, L. (2008) Rab5 and Rac Team Up in Cell Motility. Cell, 134(1), pp. 18-20. (doi: 10.1016/j.cell.2008.06.039)
2007
Thomas, S.G., Calaminus, S.D., Auger, J.M., Watson, S.P. and Machesky, L. M. (2007) Studies on the actin-binding protein HS1 in platelets. BMC Cell Biology, 8, 46. (doi: 10.1186/1471-2121-8-46) (PMID:17996076) (PMCID:PMC2203996)
Calaminus, S. D. J., Auger, J. M., McCarty, O. J. T., Wakelam, M. J. O., Machesky, L. M. and Watson, S. P. (2007) MyosinIIa contractility is required for maintenance of platelet structure during spreading on collagen and contributes to thrombus stability. Journal of Thrombosis and Haemostasis, 5(10), pp. 2136-2145. (doi: 10.1111/j.1538-7836.2007.02696.x) (PMID:17645784)
Bacon, C., Lakics, V., Machesky, L. and Rumsby, M. (2007) N-WASP regulates extension of filopodia and processes by oligodendrocyte progenitors, oligodendrocytes, and Schwann cells—implications for axon ensheathment at myelination. Glia, 55(8), pp. 844-858. (doi: 10.1002/glia.20505)
Fütterer, K. and Machesky, L. M. (2007) “Wunder” F-BAR domains: going from pits to vesicles. Cell, 129(4), pp. 655-657. (doi: 10.1016/j.cell.2007.05.006) (PMID:17512400)
Millard, T. H., Dawson, J. and Machesky, L. M. (2007) Characterisation of IRTKS, a novel IRSp53/MIM family actin regulator with distinct filament bundling properties. Journal of Cell Science, 120(9), pp. 1663-1672. (doi: 10.1242/jcs.001776) (PMID:17430976)
Calaminus, S.D.J., McCarty, O.J.T., Auger, J.M., Pearce, A.C., Insall, R.H., Watson, S.P. and Machesky, L.M. (2007) A major role for Scar/WAVE-1 downstream of GPVI in platelets. Journal of Thrombosis and Haemostasis, 5(3), pp. 535-541. (doi: 10.1111/j.1538-7836.2007.02377.x) (PMID:17319906) (PMCID:PMC1880834)
Legg, J. A., Bompard, G., Dawson, J., Morris, H. L., Andrew, N., Cooper, L., Johnston, S. A., Tramountanis, G. and Machesky, L. (2007) N-WASP involvement in dorsal ruffle formation in mouse embryonic fibroblasts. Molecular Biology of the Cell, 18(2), pp. 678-687. (doi: 10.1091/mbc.e06-06-0569) (PMID:17182853) (PMCID:PMC1783773)
Bathe, F. S., Rommelaere, H. and Machesky, L. M. (2007) Phenotypes of myopathy-related actin mutants in differentiated C2C12 myotubes. BMC Cell Biology, 8, 2. (doi: 10.1186/1471-2121-8-2) (PMID:17227580) (PMCID:PMC1779783)
Uhrig, J. F., Mutondo, M., Zimmermann, L., Deeks, M. J., Machesky, L. M. , Thomas, P., Uhrig, S., Rambke, C., Hussey, P. J. and Hulskamp, M. (2007) The role of Arabidopsis SCAR genes in ARP2-ARP3-dependent cell morphogenesis. Development, 134(5), pp. 967-977. (doi: 10.1242/dev.02792) (PMID:17267444)
Machesky, L. M. and Johnston, S. A. (2007) MIM: a multifunctional scaffold protein. Journal of Molecular Medicine, 85, pp. 569-576. (doi: 10.1007/s00109-007-0207-0) (PMID:17497115)
2006
Baldassarre, M., Ayala, I., Beznoussenko, G., Giacchetti, G., Machesky, L. M. , Luini, A. and Buccione, R. (2006) Actin dynamics at sites of extracellular matrix degradation. European journal of cell biology, 85(12), pp. 1217-1231. (doi: 10.1016/j.ejcb.2006.08.003) (PMID:17010475)
Dawson, J. C., Legg, J. A. and Machesky, L. M. (2006) Bar domain proteins: a role in tubulation, scission and actin assembly in clathrin-mediated endocytosis. Trends in cell biology, 16(10), pp. 493-498. (doi: 10.1016/j.tcb.2006.08.004) (PMID:16949824)
Machesky, L. M. , Legg, J. A., McCarty, O. J., Calaminus, S. D.J. and Watson, S. P. (2006) Scar/WAVE Proteins in Cell Spreading and Membrane Dynamics. 67th Annual Meeting of the Society of General Physiologists, Woods Hole, MA, USA, 6-10 September 2006. (doi: 10.1085/jgp.128.1.1a)
ten Klooster, J. P., Evers, E. E., Janssen, L., Machesky, L. M. , Michiels, F., Hordijk, P. and Collard, J. G. (2006) Interaction between Tiam1 and the Arp2/3 complex links activation of Rac to actin polymerization. Biochemical Journal, 397(1), pp. 39-45. (doi: 10.1042/BJ20051957) (PMID:16599904) (PMCID:PMC1479755)
McCarty, O. J. T., Calaminus, S. D. J., Berndt, M. C., Machesky, L. M. and Watson, S. P. (2006) von Willebrand factor mediates platelet spreading through glycoprotein Ib and αIIbβ3 in the presence of botrocetin and ristocetin, respectively. Journal of Thrombosis and Haemostasis, 4(6), pp. 1367-1378. (doi: 10.1111/j.1538-7836.2006.01966.x) (PMID:16706984)
Ardern, H., Sandilands, E., Machesky, L.M. , Timpson, P., Frame, M.C. and Brunton, V.G. (2006) Src-dependent phosphorylation of Scar1 promotes its association with the Arp2/3 complex. Cell Motility and the Cytoskeleton, 63(1), pp. 6-13. (doi: 10.1002/cm.20101)
2005
Deeks, M. J., Cvrcková, F., Machesky, L. M. , Mikitová, V., Ketelaar, T., Zársky, V., Davies, B. and Hussey, P. J. (2005) Arabidopsis group le formins localize to specific cell membrane domains, interact with actin-binding proteins and cause defects in cell expansion upon aberrant expression. New Phytologist, 168(3), pp. 529-540. (doi: 10.1111/j.1469-8137.2005.01582.x) (PMID:16313636)
Bompard, G., Sharp, S. J., Freiss, G. and Machesky, L. M. (2005) Involvement of Rac in actin cytoskeleton rearrangements induced by MIM-B. Journal of Cell Science, 118(22), pp. 5393-5403. (doi: 10.1242/jcs.02640) (PMID:16280553)
McCarty, O. J. T. et al. (2005) Rac1 is essential for platelet lamellipodia formation and aggregate stability under flow. Journal of Biological Chemistry, 280(47), pp. 39474-39484. (doi: 10.1074/jbc.M504672200) (PMID:16195235) (PMCID:PMC1395485)
Deeks, M.J., Kaloriti, D., Machesky, L.M. , Malho, R., Davies, B. and Hussey, P.J. (2005) Actin Nucleators in Plants. Society for Experimental Biology Annual Main Meeting, Barcelona, Spain, 11-15 July 2005. (doi: 10.1016/j.cbpb.2005.05.026)
Machesky, L.M. , Millard, T.H., Bompard, G., Sharp, S.J., Woodings, J.A. and Futterer, K. (2005) Regulation of the Actin Cytoskeleton by IRSp53 and MIM Proteins. IUBMB 50th Anniversary Symposium: Protein Structureand Function, Budapest, Hungary, 2–7 July 2005.
Owen, J. et al. (2005) Rac1 is Essential for Platelet Lamellipodia and Aggregate Stability Under Flow. BSCR Spring Meeting 2005
Oda, A. et al. (2005) WAVE/Scars in platelets. Blood, 105(8), pp. 3141-3148. (doi: 10.1182/blood-2003-04-1319) (PMID:15280206)
Stovold, C. F., Sharp, S. J. and Machesky, L. (2005) Actin filament assembly: the search for a barbed end. In: Ridley, Anne, Peckham, Michelle and Clark, Peter (eds.) Cell Motility: From Molecules to Organisms. Wiley, pp. 135-147. ISBN 9780470094099
Parsons, M. et al. (2005) Spatially distinct binding of Cdc42 to PAK1 and N-WASP in breast carcinoma cells. Molecular and Cellular Biology, 25(5), pp. 1680-1695. (doi: 10.1128/MCB.25.5.1680-1695.2005) (PMID:15713627) (PMCID:PMC549353)
Millard, T. H., Bompard, G., Heung, M. Y., Dafforn, T. R., Scott, D. J., Machesky, L. M. and Fütterer, K. (2005) Structural basis of filopodia formation induced by the IRSp53/MIM homology domain of human IRSp53. EMBO Journal, 24(2), pp. 240-250. (doi: 10.1038/sj.emboj.7600535) (PMID:15635447) (PMCID:PMC545821)
Stovold, C. F., Millard, T. H. and Machesky, L. M. (2005) Inclusion of Scar/WAVE3 in a similar complex to Scar/WAVE1 and 2. BMC Cell Biology, 6, 11. (doi: 10.1186/1471-2121-6-11) (PMID:15752430) (PMCID:PMC555569)
2004
Legg, J. A. and Machesky, L. M. (2004) MRL proteins: leading Ena/VASP to Ras GTPases. Nature Cell Biology, 6(11), pp. 1015-1017. (doi: 10.1038/ncb1104-1015) (PMID:15516992)
Clarkson, E., Costa, C. F. and Machesky, L. M. (2004) Congenital myopathies: diseases of the actin cytoskeleton. Journal of Pathology, 204(4), pp. 407-417. (doi: 10.1002/path.1648) (PMID:15495263)
Unsworth, K. E., Way, M., McNiven, M., Machesky, L. and Holden, D. W. (2004) Analysis of the mechanisms of Salmonella-induced actin assembly during invasion of host cells and intracellular replication. Cellular microbiology, 6(11), pp. 1041-1055. (doi: 10.1111/j.1462-5822.2004.00417.x) (PMID:15469433)
McCarty, O. J. T., Zhao, Y., Andrew, N., Machesky, L. M. , Staunton, D., Frampton, J. and Watson, S. P. (2004) Evaluation of the role of platelet integrins in fibronectin-dependent spreading and adhesion. Journal of Thrombosis and Haemostasis, 2(10), pp. 1823-1833. (doi: 10.1111/j.1538-7836.2004.00925.x) (PMID:15456495)
Costa, C. F., Rommelaere, H., Waterschoot, D., Sethi, K. K., Nowak, K. J., Laing, N. G., Ampe, C. and Machesky, L. M. (2004) Myopathy mutations in α-skeletal-muscle actin cause a range of molecular defects. Journal of Cell Science, 117(15), pp. 3367-3377. (doi: 10.1242/jcs.01172) (PMID:15226407)
Insall, R. H. and Machesky, L. M. (2004) Regulation of WASP: PIP2 pipped By Toca-1? Cell, 118(2), pp. 140-141. (doi: 10.1016/j.cell.2004.07.005) (PMID:15260983)
Millard, T. H., Sharp, S. J. and Machesky, L. M. (2004) Signalling to actin assembly via the WASP (Wiskott-Aldrich syndrome protein)-family proteins and the Arp2/3 complex. Biochemical Journal, 380(1), pp. 1-17. (doi: 10.1042/bj20040176) (PMID:15040784) (PMCID:PMC1224166)
Vartiainen, M. K. and Machesky, L. M. (2004) The WASP–Arp2/3 pathway: genetic insights. Current Opinion in Cell Biology, 16(2), pp. 174-181. (doi: 10.1016/j.ceb.2004.02.004) (PMID:15196561)
Millard, T.H., Bompard, G., Sharp, S.J. and Machesky, L.M. (2004) Regulation of actin dynamics by the IRSp53 and MIM family of proteins. Molecular Biology of the Cell, 15, 387A-387A.
2003
Singh, S., Powell, D. W., Rane, M. J., Millard, T. H., Trent, J. O., Pierce, W. M., Klein, J. B., Machesky, L. M. and McLeish, K. R. (2003) Identification of the p16-Arc subunit of the Arp 2/3 complex as a substrate of MAPK-activated protein kinase 2 by proteomic analysis. Journal of Biological Chemistry, 278(38), pp. 36410-36417. (doi: 10.1074/jbc.M306428200) (PMID:12829704)
Launay, S., Giannı̀, M., Diomede, L., Machesky, L. M. , Enoug, J. and Papp, B. (2003) Enhancement of ATRA-induced cell differentiation by inhibition of calcium accumulation into the endoplasmic reticulum: cross-talk between RARα and calcium-dependent signaling. Blood, 101(8), pp. 3220-3228. (doi: 10.1182/blood-2002-09-2730) (PMID:12515718)
Launay, S., Brown, G. and Machesky, L. M. (2003) Expression of WASP and Scar1/WAVE1 actin-associated proteins is differentially modulated during differentiation of HL-60 cells. Cell Motility and the Cytoskeleton, 54(4), pp. 274-285. (doi: 10.1002/cm.10101) (PMID:12601690)
Woodings, J. A., Sharp, S. J. and Machesky, L. M. (2003) MIM-B, a putative metastasis suppressor protein, binds to actin and to protein tyrosine phosphatase delta. Biochemical Journal, 371(2), pp. 463-471. (doi: 10.1042/bj20021962) (PMID:12570871) (PMCID:PMC1223315)
Oda, A. et al. (2003) CrkL directs ASAP1 to peripheral focal adhesions. Journal of Biological Chemistry, 278(8), pp. 6456-6460. (doi: 10.1074/jbc.M210817200) (PMID:12522101)
Anderson, S. I., Behrendt, B., Machesky, L. M. , Insall, R. H. and Nash, G. B. (2003) Linked regulation of motility and integrin function in activated migrating neutrophils revealed by interference in remodelling of the cytoskeleton. Cell Motility and the Cytoskeleton, 54(2), pp. 135-146. (doi: 10.1002/cm.10091) (PMID:12529859)
Machesky, L. M. and Bornens, M. (2003) Cell structure and dynamics. Current Opinion in Cell Biology, 15(1), pp. 2-5. (doi: 10.1016/S0955-0674(02)00016-9) (PMID:12517697)
Magdalena, J., Millard, T. H., Etienne-Manneville, S., Launay, S., Warwick, H. K. and Machesky, L. M. (2003) Involvement of the Arp2/3 complex and Scar2 in Golgi polarity in scratch wound models. Molecular Biology of the Cell, 14(2), pp. 670-684. (doi: 10.1091/mbc.e02-06-0345) (PMID:12589062) (PMCID:PMC150000)
Magdalena, J., Millard, T. H. and Machesky, L. M. (2003) Microtubule involvement in NIH 3T3 Golgi and MTOC polarity establishment. Journal of Cell Science, 116(4), pp. 743-756. (doi: 10.1242/jcs.00288) (PMID:12538774)
Millard, T. H., Behrendt, B., Launay, S., Fütterer, K. and Machesky, L. M. (2003) Identification and characterisation of a novel human isoform of Arp2/3 complex subunit p16-ARC/ARPC5. Cell Motility and the Cytoskeleton, 54(1), pp. 81-90. (doi: 10.1002/cm.10087) (PMID:12451597)
2002
Rombouts, K., Knittel, T., Machesky, L. , Braet, F., Wielant, A., Hellemans, K., De Bleser, P., Gelman, I., Ramadori, G. and Geerts, A. (2002) Actin filament formation, reorganization and migration are impaired in hepatic stellate cells under influence of trichostatin A, a histone deacetylase inhibitor. Journal of Hepatology, 37(6), pp. 788-796. (doi: 10.1016/S0168-8278(02)00275-1) (PMID:12445420)
Olazabal, I. M., Caron, E., May, R. C., Schilling, K., Knecht, D. A. and Machesky, L. M. (2002) Rho-kinase and myosin-II control phagocytic cup formation during CR, but not FcγR, phagocytosis. Current Biology, 12(16), pp. 1413-1418. (doi: 10.1016/S0960-9822(02)01069-2) (PMID:12194823)
Machesky, L. M. (2002) Sharks' teeth and dunes. Nature, 417, pp. 495-497. 6888. (doi: 10.1038/417494a) (PMID:12037547)
Machesky, L. and Burd, G. (2002) Cytoskeleton signalling: 7 November 2001. Biochemist, 24(1), p. 32. (doi: 10.1042/BIO02401032)
2001
Insall, R. , Müller-Taubenberger, A., Machesky, L. , Köhler, J., Simmeth, E., Atkinson, S. J., Weber, I. and Gerisch, G. (2001) Dynamics of the Dictyostelium Arp2/3 complex in endocytosis, cytokinesis, and chemotaxis. Cell Motility and the Cytoskeleton, 50(3), pp. 115-128. (doi: 10.1002/cm.10005) (PMID:11807934)
Elliott, D. A., Coleman, D. J., Lane, M. A., May, R. C., Machesky, L. M. and Clark, D. P. (2001) Cryptosporidium parvum infection requires host cell actin polymerization. Infection and Immunity, 69(9), pp. 5940-5942. (doi: 10.1128/iai.69.9.5940-5942.2001) (PMID:11500478) (PMCID:PMC98718)
May, R. C. and Machesky, L. M. (2001) Plagiarism and pathogenesis: common themes in actin remodeling. Developmental Cell, 1(3), pp. 317-318. (doi: 10.1016/S1534-5807(01)00051-X) (PMID:11702942)
Olazabal, I. M. and Machesky, L. M. (2001) Abp1p and cortactin, new “hand-holds” for actin. Journal of Cell Biology, 154(4), pp. 679-682. (doi: 10.1083/jcb.200105061) (PMID:11514584) (PMCID:PMC2196464)
Burns, S., Thrasher, A. J., Blundell, M. P., Machesky, L. and Jones, G. E. (2001) Configuration of human dendritic cell cytoskeleton by Rho GTPases, the WAS protein, and differentiation. Blood, 98(4), pp. 1142-1149. (doi: 10.1182/blood.v98.4.1142) (PMID:11493463)
Machesky, L. M. , Insall, R. H. and Volkman, L. E. (2001) WASP homology sequences in baculoviruses. Trends in Cell Biology, 11(7), pp. 286-287. (doi: 10.1016/S0962-8924(01)02009-8) (PMID:11434350)
Bailly, M., Ichetovkin, I., Grant, W., Zebda, N., Machesky, L. M. , Segall, J. E. and Condeelis, J. (2001) The F-actin side binding activity of the Arp2/3 complex is essential for actin nucleation and lamellipod extension. Current Biology, 11(8), pp. 620-625. (doi: 10.1016/S0960-9822(01)00152-X) (PMID:11369208)
Millard, T. H. and Machesky, L. M. (2001) The Wiskott-Aldrich syndrome protein (WASP) family. Trends in Biochemical Sciences, 26(3), pp. 189-199. (doi: 10.1016/S0968-0004(01)01788-1) (PMID:11246027)
May, R.C. and Machesky, L.M. (2001) Phagocytosis and the actin cytoskeleton. Journal of Cell Science, 114(6), pp. 1061-1077. (PMID:11228151)
Stinchcombe, J. C., Barral, D. C., Mules, E. H., Booth, S., Hume, A. N., Machesky, L. M. , Seabra, M. C. and Griffiths, G. M. (2001) Rab27a is required for regulated secretion in cytotoxic T lymphocytes. Journal of Cell Biology, 152(4), pp. 825-834. (doi: 10.1083/jcb.152.4.825) (PMID:11266472) (PMCID:PMC2195783)
Timpson, P., Carragher, N. O., Machesky, L. and Frame, M. C. (2001) Association of scar with the SRC tyrosine kinase: consequences for scar regulation. British Journal of Cancer, 85, p. 22. (doi: 10.1054/bjoc.2001.1917) (PMCID:PMC2557556)
Insall, R. and Machesky, L. (2001) Cytoskeleton. Encyclopedia of Life Sciences, (doi: 10.1038/npg.els.0001252)
Machesky, L. M. and May, R. C. (2001) Arps: actin-related proteins. In: dos Remedios, G. and Thomas, D.D. (eds.) Molecular Interactions of Actin. Springer. ISBN 9783540671107 (doi: 10.1007/978-3-540-46560-7_15)
Rombouts, K., Knittel, T., Wielant, A., Piscaglia, F., Machesky, L. , Hellemans, K., Gelman, I., Ramadori, G. and Feerts, A. (2001) Effect of trichostatin A, a histone deacetylase inhibitor, on proteins of the actin cytoskeleton. Cells of the Hepatic Sinusoid, 8, pp. 271-273.
2000
Pistor, S., Grobe, L., Sechi, A.S., Domann, E., Gerstel, B., Machesky, L.M. , Chakraborty, T. and Wehland, J. (2000) Mutations of arginine residues within the 146-KKRRK-150 motif of the ActA protein of Listeria monocytogenes abolish intracellular motility by interfering with the recruitment of the Arp2/3 complex. Journal of Cell Science, 113(18), pp. 3277-3287. (PMID:10954425)
Machesky, L. M. (2000) Putting on the brakes: a negative regulatory function for Ena/VASP proteins in cell migration. Cell, 101(7), pp. 685-688. (doi: 10.1016/S0092-8674(00)80879-X) (PMID:10892738)
May, R. C., Caron, E., Hall, A. and Machesky, L. M. (2000) Involvement of the Arp2/3 complex in phagocytosis mediated by FcγR or CR3. Nature Cell Biology, 2(4), pp. 246-248. (doi: 10.1038/35008673) (PMID:10783245)
Rozelle, A.L., Machesky, L.M. , Yamamoto, M., Driessens, M.H.E., Insall, R.H. , Roth, M.G., Luby-Phelps, K., Marriott, G., Hall, A. and Yin, H.L. (2000) Phosphatidylinositol 4,5-bisphosphate induces actin-based movement of raft-enriched vesicles through WASP-Arp2/3. Current Biology, 10(6), pp. 311-320. (doi: 10.1016/S0960-9822(00)00384-5) (PMID:10744973)
Machesky, L. M. (2000) The tails of two myosins. Journal of Cell Biology, 148(2), pp. 219-221. (doi: 10.1083/jcb.148.2.219) (PMID:10648552) (PMCID:PMC2174283)
Machesky, L. M. and Schliwa, M. (2000) Cell dynamics: a new look at the cytoskeleton. Nature Cell Biology, 2(1), E17-E18. (doi: 10.1038/71403) (PMID:10620818)
Machesky, L. M. , May, R., Olarreaga, I. M. O., Launay, S., Hall, M. E. and Behrendt, B. (2000) Signalling to the actin cytoskeleton via the WASP-family proteins and the Arp2/3 complex. Molecular Biology of the Cell, 11, 3A.
Mullins, R. D. and Machesky, L. M. (2000) Actin assembly mediated by Arp2/3 complex and WASP family proteins. Methods in Enzymology, 325, pp. 214-237. (doi: 10.1016/s0076-6879(00)25445-1) (PMID:11036606)
1999
Machesky, L. M. and Cooper, J. A. (1999) Cell motility: Bare bones of the cytoskeleton. Nature, 401(6753), pp. 542-543. (doi: 10.1038/44044) (PMID:10524617)
Machesky, L. (1999) Short and not so sweet. Current Biology, 9(17), R623. (doi: 10.1016/s0960-9822(99)80403-5) (PMID:10508596)
Machesky, L. M. and Insall, R. H. (1999) Signaling to actin dynamics. Journal of Cell Biology, 146(2), pp. 267-272. (doi: 10.1083/jcb.146.2.267) (PMID:10427083) (PMCID:PMC2156183)
May, R. C., Hall, M. E., Higgs, H. N., Pollard, T. D., Chakraborty, T., Wehland, J., Machesky, L. M. and Sechi, A. S. (1999) The Arp2/3 complex is essential for the actin-based motility of Listeria monocytogenes. Current Biology, 9(14), pp. 759-762. (doi: 10.1016/S0960-9822(99)80337-6) (PMID:10421578)
Insall, R. and Machesky, L. (1999) PH domains in WASP – a bug in the system? Trends in Cell Biology, 9(6), p. 211. (doi: 10.1016/S0962-8924(99)01569-X) (PMID:10354565)
Machesky, L. M. (1999) Rocket-based motility: a universal mechanism? Nature Cell Biology, 1(2), E29-E31. (doi: 10.1038/10020) (PMID:10559888)
Castellano, F., Montcourrier, P., Guillemot, J.-C., Gouin, E., Machesky, L. , Cossart, P. and Chavrier, P. (1999) Inducible recruitment of Cdc42 or WASP to a cell-surface receptor triggers actin polymerization and filopodium formation. Current Biology, 9(7), pp. 351-361. (doi: 10.1016/S0960-9822(99)80161-4) (PMID:10209117)
Tsujioka, M., Machesky, L.M. , Cole, S.L., Yahata, K. and Inouye, K. (1999) A unique talin homologue with a villin headpiece-like domain is required for multicellular morphogenesis in Dictyostelium. Current Biology, 9(7), pp. 389-394. (doi: 10.1016/S0960-9822(99)80169-9) (PMID:10209124)
Machesky, L. M. , Mullins, R. D., Higgs, H. N., Kaiser, D. A., Blanchoin, L., May, R. C., Hall, M. E. and Pollard, T. D. (1999) Scar, a WASp-related protein, activates nucleation of actin filaments by the Arp2/3 complex. Proceedings of the National Academy of Sciences of the United States of America, 96(7), pp. 3739-3744. (doi: 10.1073/pnas.96.7.3739) (PMID:10097107) (PMCID:PMC22364)
Machesky, L. M. and Gould, K. L. (1999) The Arp2/3 complex: a multifunctional actin organizer. Current Opinion in Cell Biology, 11(1), pp. 117-121. (doi: 10.1016/S0955-0674(99)80014-3) (PMID:10047519)
Braga, V. M.M., Del Maschio, A., Machesky, L. and Dejana, E. (1999) Regulation of cadherin function by Rho and Rac: modulation by junction maturation and cellular context. Molecular Biology of the Cell, 10(1), pp. 9-22. (doi: 10.1091/mbc.10.1.9) (PMID:9880323) (PMCID:PMC25150)
Rombouts, K., Knittel, T., Wielant, A., Hellemans, K., Gelman, I., Machesky, L. , Ramadori, G. and Gerts, A. (1999) Trichostatin A suppresses transdifferentiation of rat stellate cells by its effect on the assembly pathway of the actin cytoskeleton. Hepatology, 30(4), 492A.
1998
Machesky, L. M. and Insall, R. H. (1998) Scar1 and the related Wiskott–Aldrich syndrome protein, WASP, regulate the actin cytoskeleton through the Arp2/3 complex. Current Biology, 8(25), pp. 1347-1356. (doi: 10.1016/S0960-9822(98)00015-3) (PMID:9889097)
Schafer, D. A., Welch, M. D., Machesky, L. M. , Bridgman, P. C., Meyer, S. M. and Cooper, J. A. (1998) Visualization and molecular analysis of actin assembly in living cells. Journal of Cell Biology, 143(7), pp. 1919-1930. (doi: 10.1083/jcb.143.7.1919) (PMID:9864364) (PMCID:PMC2175235)
Machesky, L. M. and Way, M. (1998) Actin branches out. Nature, 394(6689), pp. 125-126. (doi: 10.1038/28039) (PMID:9671292)
Machesky, L. M. , Insall, R. H. and Kay, R. R. (1998) The helC gene encodes a putative DEAD-box RNA helicase required for development in Dictyostelium discoideum. Current Biology, 8(10), 607-S4. (doi: 10.1016/S0960-9822(98)70233-7) (PMID:9601648)
Machesky, L. M. (1998) Cytokinesis: IQGAPs find a function. Current Biology, 8(6), R202-R205. (doi: 10.1016/s0960-9822(98)70125-3) (PMID:9512410)
1997
Machesky, L. M. , Reeves, E., Wietnjes, F., Mattheyse, F. J., Grogan, A., Totty, N. F., Burlingame, A. L., Hsuan, J. J. and Segal, A. W. (1997) Mammalian actin-related protein 2/3 complex localizes to regions of lamellipodial protrusion and is composed of evolutionarily conserved proteins. Biochemical Journal, 328(1), pp. 105-112. (doi: 10.1042/bj3280105) (PMID:9359840) (PMCID:PMC1218893)
Machesky, L. M. and Hall, A. (1997) Role of actin polymerization and adhesion to extracellular matrix in Rac-and Rho-induced cytoskeletal reorganization. Journal of Cell Biology, 138(4), pp. 913-926. (doi: 10.1083/jcb.138.4.913) (PMID:9265656) (PMCID:PMC2138040)
Tuxworth, R. I., Cheetham, J. L., Machesky, L. M. , Spiegelmann, G. B., Weeks, G. and Insall, R. H. (1997) Dictyostelium RasG is required for normal motility and cytokinesis, but not growth. Journal of Cell Biology, 138(3), pp. 605-614. (doi: 10.1083/jcb.138.3.605) (PMID:9245789) (PMCID:PMC2141629)
Braga, V. M.M., Machesky, L. M. , Hall, A. and Hotchin, N. A. (1997) The small GTPases Rho and Rac are required for the establishment of cadherin-dependent cell-cell contacts. Journal of Cell Biology, 137(6), pp. 1421-1431. (doi: 10.1083/jcb.137.6.1421) (PMID:9182672) (PMCID:PMC2132529)
Machesky, L. M. (1997) Cell motility: complex dynamics at the leading edge. Current Biology, 7(3), R164-R167. (doi: 10.1016/S0960-9822(97)70079-4) (PMID:9162480)
1996
Machesky, L. M. and Hall, A. (1996) Rho: a connection between membrane receptor signalling and the cytoskeleton. Trends in Cell Biology, 6(8), pp. 304-310. (doi: 10.1016/0962-8924(96)10026-X) (PMID:15157438)
Petrella, E. C., Machesky, L. M. , Kaiser, D. A. and Pollard, T. D. (1996) Structural requirements and thermodynamics of the interaction of proline peptides with profilin. Biochemistry, 35(51), pp. 16535-16543. (doi: 10.1021/bi961498d) (PMID:8987987)
1995
Doberstein, S.K., Wiegand, G., Machesky, L.M. and Pollard, T.D. (1995) Fluorescent erythrocyte ghosts as standards for quantitative flow cytometry. Cytometry, 20(1), pp. 14-18. (doi: 10.1002/cyto.990200104) (PMID:7600895)
1994
Machesky, L.M. , Atkinson, S.J., Ampe, C., Vandekerckhove, J. and Pollard, T.D. (1994) Purification of a cortical complex containing two unconventional actins from Acanthamoeba by affinity chromatography on profilin-agarose. Journal of Cell Biology, 127(1), pp. 107-115. (doi: 10.1083/jcb.127.1.107) (PMID:7929556) (PMCID:PMC2120189)
Machesky, L. M. , Cole, N. B., Moss, B. and Pollard, T. D. (1994) Vaccinia virus expresses a novel profilin with a higher affinity for polyphosphoinositides than actin. Biochemistry, 33(35), pp. 10815-10824. (doi: 10.1021/bi00201a032) (PMID:8075084)
1993
Machesky, L. M. and Pollard, T. D. (1993) Profilin as a potential mediator of membrane-cytoskeleton communication. Trends in Cell Biology, 3(11), pp. 381-385. (doi: 10.1016/0962-8924(93)90087-h) (PMID:14731655)
1992
Machesky, L.M. (1992) Phosphorylation of phospholipase-γ l, profilin and tyrosine. Biomedicine and Pharmacotherapy, 46(2-3), pp. 128-129. (doi: 10.1016/0753-3322(92)90287-H)
1991
Goldschmidt-Clermont, P.J., Machesky, L.M. , Doberstein, S.K. and Pollard, T.D. (1991) Mechanism of the interaction of human platelet profilin with actin. Journal of Cell Biology, 113(5), pp. 1081-1089. (doi: 10.1083/jcb.113.5.1081) (PMID:1645736) (PMCID:PMC2289016)
Goldschmidt-Clermont, P.J., Kim, J.W., Machesky, L.M. , Rhee, S.G. and Pollard, T.D. (1991) Regulation of phospholipase C-gamma 1 by profilin and tyrosine phosphorylation. Science, 251(4998), pp. 1231-1233. (doi: 10.1126/science.1848725) (PMID:1848725)
Pollard, T. D., Machesky, L. and Goldschmidt-Clermont, P. (1991) Interaction of profilins with membrane lipids. In: Mooseker, Mark S. and Morrow, Jon S. (eds.) Ordering the Membrane-Cytoskeleton Trilayer. Series: Current Topics in Membranes (38). Academic Press: San Diego, California ; London, pp. 217-225. ISBN 9780121533380 (doi: 10.1016/S0070-2161(08)60790-X)
1990
Machesky, L.M. , Goldschmidt-Clermont, P.J. and Pollard, T.D. (1990) The affinities of human platelet and Acanthamoeba profilin isoforms for polyphosphoinositides account for their relative abilities to inhibit phospholipase C. Cell Regulation, 1(12), pp. 937-950. (doi: 10.1091/mbc.1.12.937) (PMID:1966040) (PMCID:PMC362863)
Goldschmidt-Clermont, P.J., Machesky, L.M. , Baldassare, J.J. and Pollard, T.D. (1990) The actin-binding protein profilin binds to PIP2 and inhibits its hydrolysis by phospholipase C. Science, 247(4950), pp. 1575-1578. (doi: 10.1126/science.2157283) (PMID:2157283)
1989
Pollard, T. D., Magnus, K. A., Doberstein, S., Goldschmidt-Clermont, P., Kaiser, D. A., Machesky, L. , Maciver, S., Rimm, D. L. and Wachsstock, D. (1989) Structure-function studies of the actin filament system of Acanthamoeba. In: Aebi, Ueli and Engel, Jürgen (eds.) Cytoskeletal and Extracellular Proteins: Structure, Interactions and Assembly The 2nd International EBSA Symposium. Series: Springer series in biophysics (3). Springer: Berlin, Heidelberg, pp. 271-279. ISBN 9783642739279 (doi: 10.1007/978-3-642-73925-5_50)
1988
Kang, Z.C., Machesky, L. , Eick, H.A. and Eyring, L. (1988) The solvolytic disproportionation of mixed-valence compounds: III. Pb3O4. Journal of Solid State Chemistry, 75(1), pp. 73-89. (doi: 10.1016/0022-4596(88)90304-0)
Articles
Nikolaou, S., Juin, A., Whitelaw, J. A., Paul, N. R., Fort, L., Nixon, C., Spence, H. J., Bryson, S. and Machesky, L. M. (2024) CYRI-B-mediated macropinocytosis drives metastasis via lysophosphatidic acid receptor uptake. eLife, 13, e83712. (doi: 10.7554/elife.83712) (PMID:38712822) (PMCID:PMC11219039)
Kapitany, V., Fatima, A., Zickus, V., Whitelaw, J., McGhee, E., Insall, R. , Machesky, L. and Faccio, D. (2024) Single-sample image-fusion upsampling of fluorescence lifetime images. Science Advances, 10(21), eadn0139. (doi: 10.1126/sciadv.adn0139)
Juin, A., Spence, H. J. and Machesky, L. M. (2024) Dichotomous role of the serine/threonine kinase MAP4K4 in pancreatic ductal adenocarcinoma onset and metastasis through control of AKT and ERK pathways. Journal of Pathology, 262(4), pp. 454-466. (doi: 10.1002/path.6248) (PMID:38229581)
Morris, H. T. , Bamlet, W. R., Razidlo, G. L. and Machesky, L. M. (2023) FSCN1 and epithelial mesenchymal transformation transcription factor expression in human pancreatic intraepithelial neoplasia and ductal adenocarcinoma. Pathology: Research and Practice, 251, 154836. (doi: 10.1016/j.prp.2023.154836) (PMID:37832352) (PMCID:37832352)
Machesky, L. M. (2023) CYRI proteins: controllers of actin dynamics in the cellular ‘eat vs walk’ decision. Biochemical Society Transactions, 51(2), pp. 579-585. (doi: 10.1042/bst20221354) (PMID:36892409)
Wilkinson, E. L. et al. (2023) Genetically engineered multicistronic allele of Pmel yielding highly specific CreERT2-mediated recombination in the melanocyte lineage. Pigment Cell and Melanoma Research, 36(1), pp. 71-77. (doi: 10.1111/pcmr.13076) (PMID:36412082)
Papalazarou, V., Drew, J., Juin, A., Spence, H. J., Whitelaw, J., Nixon, C., Salmeron-Sanchez, M. and Machesky, L. M. (2022) Collagen VI expression is negatively mechanosensitive in pancreatic cancer cells and supports the metastatic niche. Journal of Cell Science, 135(24), jcs259978. (doi: 10.1242/jcs.259978) (PMID:36546396) (PMCID:PMC9845737)
Le, A. H., Yelland, T., Paul, N. R., Fort, L., Nikolaou, S., Ismail, S. and Machesky, L. M. (2021) CYRI-A limits invasive migration through macropinosome formation and integrin uptake regulation. Journal of Cell Biology, 220(9), e202012114. (doi: 10.1083/jcb.202012114) (PMID:34165494) (PMCID:PMC8236918)
Olson, M. F. and Machesky, L. M. (2021) MICAL2 fine-tunes Arp2/3 for actin branching. Journal of Cell Biology, 220(8), e202106176. (doi: 10.1083/jcb.202106176) (PMID:34264264)
Drew, J. and Machesky, L. M. (2021) The liver metastatic niche: modelling the extracellular matrix in metastasis. Disease Models and Mechanisms, 14(4), dmm048801. (doi: 10.1242/dmm.048801) (PMID:33973625) (PMCID:PMC8077555)
Yelland, T., Le, A. H., Nikolaou, S., Insall, R. , Machesky, L. and Ismail, S. (2021) Structural basis of CYRI-B direct competition with Scar/WAVE complex for Rac1. Structure, 29(3), 226-237.e4. (doi: 10.1016/j.str.2020.11.003) (PMID:33217330) (PMCID:PMC7955166)
Swaminathan, K., Campbell, A., Papalazarou, V., Jaber-Hijazi, F. , Nixon, C., McGhee, E., Strathdee, D., Sansom, O. J. and Machesky, L. M. (2021) The RAC1 target NCKAP1 plays a crucial role in the progression of Braf;Pten-driven melanoma in mice. Journal of Investigative Dermatology, 141(3), 628-637.e15. (doi: 10.1016/j.jid.2020.06.029) (PMID:32777214)
Callenberg, C., Lyons, A. , den Brok, D., Fatima, A., Turpin, A. , Zickus, V., Machesky, L. , Whitelaw, J., Faccio, D. and Hullin, M.B. (2021) Super-resolution time-resolved imaging using computational sensor fusion. Scientific Reports, 11, 1689. (doi: 10.1038/s41598-021-81159-x) (PMID:33462284) (PMCID:PMC7813875)
Pickering, K.A. et al. (2021) A RAC-GEF network critical for early intestinal tumourigenesis. Nature Communications, 12, 56. (doi: 10.1038/s41467-020-20255-4) (PMID:33397922) (PMCID:PMC7782582)
Zickus, V. et al. (2020) Fluorescence lifetime imaging with a megapixel SPAD camera and neural network lifetime estimation. Scientific Reports, 10, 20986. (doi: 10.1038/s41598-020-77737-0) (PMID:33268900) (PMCID:PMC7710711)
Papalazarou, V., Swaminathan, K., Jaber-Hijazi, F. , Spence, H., Lahmann, I., Nixon, C., Salmeron-Sanchez, M. , Arnold, H.-H., Rottner, K. and Machesky, L. M. (2020) The Arp2/3 complex is crucial for colonisation of the mouse skin by melanoblasts. Development, 147(22), dev194555. (doi: 10.1242/dev.194555) (PMID:33028610) (PMCID:PMC7687863)
Tweedy, L., Thomason, P. A., Paschke, P. I., Martin, K., Machesky, L. M. , Zagnoni, M. and Insall, R. H. (2020) Seeing around corners: cells solve mazes and respond at a distance using attractant breakdown. Science, 369(6507), eaay9792. (doi: 10.1126/science.aay9792) (PMID:32855311)
Singh, S. P. , Thomason, P. A., Lilla, S., Schaks, M., Tang, Q., Goode, B. L., Machesky, L. M. , Rottner, K. and Insall, R. H. (2020) Cell–substrate adhesion drives Scar/WAVE activation and phosphorylation by a Ste20-family kinase, which controls pseudopod lifetime. PLoS Biology, 18(8), e3000774. (doi: 10.1371/journal.pbio.3000774) (PMID:32745097) (PMCID:PMC7425996)
McMenamin, P. G., Shields, G. T., Seyed-Razavi, Y., Kalirai, H., Insall, R. H. , Machesky, L. M. and Coupland, S. E. (2020) Melanoblasts populate the mouse choroid earlier in development than previously described. Investigative Ophthalmology and Visual Science, 61(10), 33. (doi: 10.1167/iovs.61.10.33) (PMID:32797202) (PMCID:PMC7441366)
Whitelaw, J. A., Swaminathan, K., Kage, F. and Machesky, L. M. (2020) The WAVE regulatory complex is required to balance protrusion and adhesion in migration. Cells, 9(7), 1635. (doi: 10.3390/cells9071635) (PMID:32646006) (PMCID:PMC7407199)
Nikolaou, S. and Machesky, L. (2020) The stressful tumour environment drives plasticity of cell migration programmes, contributing to metastasis. Journal of Pathology, 250(5), pp. 612-623. (doi: 10.1002/path.5395) (PMID:32057095) (PMCID:PMC7216910)
Papalazarou, V., Zhang, T., Paul, N. R., Juin, A., Cantini, M. , Maddocks, O. D.K. , Salmeron-Sanchez, M. and Machesky, L. (2020) The creatine-phosphagen system is mechanoresponsive in pancreatic adenocarcinoma and fuels invasion and metastasis. Nature Metabolism, 2(1), pp. 62-80. (doi: 10.1038/s42255-019-0159-z) (PMID:32694686)
Schurr, Y. et al. (2019) Platelet lamellipodium formation is not required for thrombus formation and stability. Blood, 134(25), pp. 2318-2329. (doi: 10.1182/blood.2019002105) (PMID:31697813)
Amato, C., Thomason, P. A., Davidson, A. J., Swaminathan, K., Ismail, S. , Machesky, L. M. and Insall, R. H. (2019) WASP restricts active Rac to maintain cells’ front-rear polarization. Current Biology, 29(24), pp. 4169-4182. (doi: 10.1016/j.cub.2019.10.036) (PMID:31786060) (PMCID:PMC6926487)
Juin, A. et al. (2019) N-WASP control of LPAR1 trafficking establishes response to self-generated LPA gradients to promote pancreatic cancer cell metastasis. Developmental Cell, 51(4), 431-445.e7. (doi: 10.1016/j.devcel.2019.09.018) (PMID:31668663) (PMCID:PMC6863394)
Francis, S. et al. (2019) Structure-based design, synthesis and biological evaluation of a novel series of isoquinolone and pyrazolo[4,3-c]pyridine inhibitors of fascin 1 as potential anti-metastatic agents. Bioorganic and Medicinal Chemistry Letters, 29(8), pp. 1023-1029. (doi: 10.1016/j.bmcl.2019.01.035) (PMID:30773430) (PMCID:PMC6419574)
Nikolaou, S. and Machesky, L. M. (2019) Invasion by force: The C. elegans anchor cell leads the way. Developmental Cell, 48(3), pp. 287-288. (doi: 10.1016/j.devcel.2019.01.024)
Kugeratski, F. G. et al. (2019) Hypoxic cancer–associated fibroblasts increase NCBP2-AS2/HIAR to promote endothelial sprouting through enhanced VEGF signaling. Science Signaling, 12(567), eaan8247. (doi: 10.1126/scisignal.aan8247) (PMID:30723174) (PMCID:PMC6794160)
Machesky, L. M. (2019) Rab11FIP proteins link endocytic recycling vesicles for cytoskeletal transport and tethering. Bioscience Reports, 39(1), BSR2018221. (doi: 10.1042/bsr20182219) (PMID:30622149) (PMCID:PMC6356010)
Bachg, A. C. et al. (2019) Phenotypic analysis of Myo10 knockout (Myo10tm2/tm2) mice lacking full-length (motorized) but not brain-specific headless myosin X. Scientific Reports, 9, 597. (doi: 10.1038/s41598-018-37160-y) (PMID:30679680) (PMCID:PMC6345916)
Papalazarou, V., Salmeron-Sanchez, M. and Machesky, L. M. (2018) Tissue engineering the cancer microenvironment—challenges and opportunities. Biophysical Reviews, 10(6), pp. 1695-1711. (doi: 10.1007/s12551-018-0466-8) (PMID:30406572) (PMCID:PMC6297082)
Fort, L. et al. (2018) Fam49/CYRI interacts with Rac1 and locally suppresses protrusions. Nature Cell Biology, 20(10), pp. 1159-1171. (doi: 10.1038/s41556-018-0198-9) (PMID:30250061) (PMCID:PMC6863750)
Morris, H. T. , Fort, L., Spence, H. J., Patel, R., Vincent, D. F., Park, J. H. , Snapper, S. B., Carey, F. A., Sansom, O. J. and Machesky, L. M. (2018) Loss of N-WASP drives early progression in an Apc model of intestinal tumourigenesis. Journal of Pathology, 245(3), pp. 337-348. (doi: 10.1002/path.5086) (PMID:29672847) (PMCID:PMC6033012)
Laurent, P., Petit, V., Aktary, Z., Gallagher, S., Tweedy, L., Machesky, L. and Larue, L. (2018) Simulation of melanoblast displacements reveals new features of developmental migration. Development, 145(12), dev160200. (doi: 10.1242/dev.160200) (PMID:29769218) (PMCID:PMC6031402)
Machesky, L. and Braga, V. M.M. (2017) So far, yet so close: α-Catenin dimers help migrating cells get together. Journal of Cell Biology, 216(11), pp. 3437-3439. (doi: 10.1083/jcb.201709056) (PMID:29051263)
Giampazolias, E. et al. (2017) Mitochondrial permeabilization engages NF-κB-dependent anti-tumour activity under caspase deficiency. Nature Cell Biology, 19(9), pp. 1116-1129. (doi: 10.1038/ncb3596) (PMID:28846096) (PMCID:PMC5624512)
Reid, S. E. et al. (2017) Tumor matrix stiffness promotes metastatic cancer cell interaction with the endothelium. EMBO Journal, 36(16), pp. 2373-2389. (doi: 10.15252/embj.201694912) (PMID:28694244) (PMCID:PMC5556271)
Woodham, E. F. et al. (2017) Coordination by Cdc42 of actin, contractility, and adhesion for melanoblast movement in mouse skin. Current Biology, 27(5), pp. 624-637. (doi: 10.1016/j.cub.2017.01.033) (PMID:28238662) (PMCID:PMC5344686)
Thomas, S. G., Poulter, N. S., Bem, D., Finney, B., Machesky, L. M. and Watson, S. P. (2017) The actin binding proteins cortactin and HS1 are dispensable for platelet actin nodule and megakaryocyte podosome formation. Platelets, 28(4), pp. 372-379. (doi: 10.1080/09537104.2016.1235688) (PMID:27778524) (PMCID:PMC5274539)
Tyrrell, B. J., Woodham, E. F., Spence, H. J., Strathdee, D., Insall, R. H. and Machesky, L. M. (2016) Loss of strumpellin in the melanocytic lineage impairs the WASH Complex but does not affect coat colour. Pigment Cell and Melanoma Research, 29(5), pp. 559-571. (doi: 10.1111/pcmr.12506) (PMID:27390154) (PMCID:PMC5082549)
Sinclair, A. et al. (2016) CXCR2 and CXCL4 regulate survival and self-renewal of hematopoietic stem/progenitor cells. Blood, 128(3), pp. 371-383. (doi: 10.1182/blood-2015-08-661785) (PMID:27222476) (PMCID:PMC4991087)
García, E., Ragazzini, C., Yu, X., Cuesta-García, E., Bernardino de la Serna, J., Zech, T., Sarrió, D., Machesky, L. M. and Antón, I. M. (2016) WIP and WICH/WIRE co-ordinately control invadopodium formation and maturation in human breast cancer cell invasion. Scientific Reports, 6, 23590. (doi: 10.1038/srep23590) (PMID:27009365) (PMCID:PMC4806363)
Ma, Y. and Machesky, L. (2015) Fascin1 in carcinomas: Its regulation and prognostic value. International Journal of Cancer, 137(11), pp. 2534-2544. (doi: 10.1002/ijc.29260) (PMID:25302416)
Siebert, S. , Machesky, L. M. and Insall, R. H. (2015) Overflow in science and its implications for trust. eLife, 4, e10825. (doi: 10.7554/eLife.10825)
Giancotti, F. G., Haynes, C. M., Machesky, L. M. , Olson, M. F. and Overholtzer, M. (2015) Alan Hall 1952–2015. Nature Cell Biology, 17(7), pp. 839-840. (doi: 10.1038/ncb3190) (PMID:26005836)
Rainero, E., Howe, J. D., Caswell, P. T., Jamieson, N. B. , Anderson, K., Critchley, D. R., Machesky, L. and Norman, J. C. (2015) Ligand-occupied integrin internalization links nutrient signaling to invasive migration. Cell Reports, 10(3), pp. 398-413. (doi: 10.1016/j.celrep.2014.12.037)
Vijayakumar, V., Monypenny, J., Chen, X. J., Machesky, L. M. , Lilla, S., Thrasher, A. J., Anton, I. M., Calle, Y. and Jones, G. E. (2015) Tyrosine phosphorylation of WIP releases bound WASP and impairs podosome assembly in macrophages. Journal of Cell Science, 128(2), pp. 251-265. (doi: 10.1242/jcs.154880)
Lindsay, C. R., Li, A., Faller, W., Ozanne, B., Welch, H., Machesky, L. M. and Sansom, O. (2015) A Rac1-independent role for P-Rex1 in melanoblasts. Journal of Investigative Dermatology, 135(1), pp. 314-318. (doi: 10.1038/jid.2014.323)
Ma, Y., Faller, W., Sansom, O. , Brown, E. R., Doig, T. N., Melton, D. W. and Machesky, L. (2015) Fascin expression is increased in metastatic lesions but does not correlate with progression nor outcome in melanoma. Melanoma Research, 25(2), pp. 169-172. (doi: 10.1097/CMR.0000000000000135) (PMID:25535872)
Morris, H.T. and Machesky, L.M. (2015) Actin cytoskeletal control during epithelial to mesenchymal transition: focus on the pancreas and intestinal tract. British Journal of Cancer, 112(4), pp. 613-620. (doi: 10.1038/bjc.2014.658) (PMID:25611303) (PMCID:PMC4333498)
Muinonen-Martin, A. J. et al. (2014) Melanoma cells break down LPA to establish local gradients that drive chemotactic dispersal. PLoS Biology, 12(10), e1001966. (doi: 10.1371/journal.pbio.1001966) (PMID:25313567) (PMCID:PMC4196730)
García, E., Machesky, L. M. , Jones, G. E. and Antón, I. M. (2014) WIP is necessary for matrix invasion by breast cancer cells. European Journal of Cell Biology, 93(10-12), pp. 413-423. (doi: 10.1016/j.ejcb.2014.07.008)
Woodham, E. F. and Machesky, L. M. (2014) Polarised cell migration: intrinsic and extrinsic drivers. Current Opinion in Cell Biology, 30, pp. 25-32. (doi: 10.1016/j.ceb.2014.05.006)
Machesky, L. and Sedwick, C. (2014) Laura Machesky: actin opens the way. Journal of Cell Biology, 206(7), pp. 816-817. (doi: 10.1083/jcb.2067pi) (PMID:25267291) (PMCID:PMC4178962)
Li, A. et al. (2014) Fascin is regulated by slug, promotes progression of pancreatic cancer in mice, and is associated with patient outcomes. Gastroenterology, 146(5), 1386-1396.e17. (doi: 10.1053/j.gastro.2014.01.046) (PMID:24462734) (PMCID:PMC4000441)
Tyrrell, B. J., Neilson, M., Insall, R. H. and Machesky, L. M. (2014) Predicting cell shapes in melanomas. Pigment Cell and Melanoma Research, 27(1), pp. 5-6. (doi: 10.1111/pcmr.12176) (PMID:24118871)
Schachtner, H., Li, A., Stevenson, D., Calaminus, S. D.J., Thomas, S. G., Watson, S. P., Sixt, M., Wedlich-Soldner, R., Strathdee, D. and Machesky, L. M. (2013) Tissue inducible Lifeact expression allows visualization of actin dynamics in vivo and ex vivo. European Journal of Cell Biology, 91(11-12), pp. 923-929. (doi: 10.1016/j.ejcb.2012.04.002)
Futó, K., Bódis, E., Machesky, L.M. , Nyitrai, M. and Visegrády, B. (2013) Membrane binding properties of IRSp53-missing in metastasis domain (IMD) protein. Biochimica et Biophysica Acta: Molecular and Cell Biology of Lipids, 1831(11), pp. 1651-1655. (doi: 10.1016/j.bbalip.2013.07.006)
Ma, Y., Reynolds, L. E., Li, A., Stevenson, R. P., Hodivala-Dilke, K. M., Yamashiro, S. and Machesky, L. M. (2013) Fascin 1 is dispensable for developmental and tumour angiogenesis. Biology Open, 2(11), pp. 1187-1191. (doi: 10.1242/bio.20136031) (PMID:24244855) (PMCID:PMC3828765)
Schachtner, H., Calaminus, S. D. J., Thomas, S. G. and Machesky, L. M. (2013) Podosomes in adhesion, migration, mechanosensing and matrix remodeling. Cytoskeleton, 70(10), pp. 572-589. (doi: 10.1002/cm.21119)
Ampe, C., Machesky, L.M. and Nyitrai, M. (2013) Biophysical approaches for investigation of the cytoskeleton. A special issue stemming from research presented at the 2012 ECF meeting: A FEBS/EMBO workshop lecture course in Pécs, Hungary. Cytoskeleton, 70(10), p. 539. (doi: 10.1002/cm.21145)
Sonego, M., Gajendra, S., Parsons, M., Ma, Y., Hobbs, C., Zentar, M.P., Williams, G., Machesky, L.M. , Doherty, P. and Lalli, G. (2013) Fascin regulates the migration of subventricular zone-derived neuroblasts in the postnatal brain. Journal of Neuroscience, 33(30), pp. 12171-12185. (doi: 10.1523/JNEUROSCI.0653-13.2013)
Ma, Y., Li, A., Faller, W.J., Libertini, S., Fiorito, F., Gillespie, D.A., Sansom, O.J. , Yamashiro, S. and Machesky, L.M. (2013) Fascin 1 is transiently expressed in mouse melanoblasts during development and promotes migration and proliferation. Development, 140(10), pp. 2203-2211. (doi: 10.1242/dev.089789)
Li, A. and Machesky, L. M. (2013) Rac1 cycling fast in melanoma with P29S. Pigment Cell and Melanoma Research, 26(3), pp. 289-290. (doi: 10.1111/pcmr.12074) (PMID:23530970)
Schachtner, H. et al. (2013) Megakaryocytes assemble podosomes that degrade matrix and protrude through basement membrane. Blood, 121(13), pp. 2542-2552. (doi: 10.1182/blood-2012-07-443457) (PMID:23305739)
Zanivan, S. et al. (2013) In Vivo SILAC-based proteomics reveals phosphoproteome changes during mouse skin carcinogenesis. Cell Reports, 3(2), pp. 552-566. (doi: 10.1016/j.celrep.2013.01.003)
Park, L., Thomason, P.A., Zech, T., King, J.S., Veltman, D.M., Carnell, M., Ura, S., Machesky, L.M. and Insall, R.H. (2013) Cyclical Action of the WASH complex: FAM21 and capping protein drive WASH recycling, not initial recruitment. Developmental Cell, 24(2), pp. 169-181. (doi: 10.1016/j.devcel.2012.12.014)
Tang, H. et al. (2013) Loss of scar/WAVE complex promotes N-WASP- and FAK-dependent invasion. Current Biology, 23(2), pp. 107-117. (doi: 10.1016/j.cub.2012.11.059)
Calaminus, S. D.J., Guitart, A. V., Sinclair, A., Schachtner, H., Watson, S. P., Holyoake, T. L., Kranc, K. R. and Machesky, L. M. (2012) Lineage tracing of Pf4-Cre marks hematopoietic stem cells and their progeny. PLoS ONE, 7(12), e51361. (doi: 10.1371/journal.pone.0051361) (PMID:23300543) (PMCID:PMC3531453)
Machesky, L.M. and Sansom, O.J. (2012) Rac1 in the driver’s seat for melanoma. Pigment Cell and Melanoma Research, 25(6), pp. 762-764. (doi: 10.1111/pcmr.12004)
Yu, X. et al. (2012) N-WASP coordinates the delivery and F-actin-mediated capture of MT1-MMP at invasive pseudopods. Journal of Cell Biology, 199(3), pp. 527-544. (doi: 10.1083/jcb.201203025)
Veltman, D. M., King, J. S., Machesky, L. M. and Insall, R. H. (2012) SCAR knockouts in Dictyostelium: WASP assumes SCAR’s position and upstream regulators in pseudopods. Journal of Cell Biology, 198(4), pp. 501-508. (doi: 10.1083/jcb.201205058) (PMID:22891261) (PMCID:PMC3514037)
Maritzen, T., Zech, T., Schmidt, M.R., Krause, E., Machesky, L.M. and Haucke, V. (2012) Gadkin negatively regulates cell spreading and motility via sequestration of the actin-nucleating ARP2/3 complex. Proceedings of the National Academy of Sciences of the United States of America, 109(26), pp. 10382-10387. (doi: 10.1073/pnas.1206468109)
Stevenson, R. P., Myant, K., Jamieson, T., Ma, Y., Li, A., Tan, E. H., Yamashiro, S., Pignatelli, M., Sansom, O. and Machesky, L. M. (2012) Targeting the Actin Cytoskeletal Protein Fascin as a Novel Modulator of Intestinal Epithelial Cell Proliferation and Colon Cancer. Gastroenterology, 142(5 Sup1), S-44. (doi: 10.1016/S0016-5085(12)60167-X)
Ura, S., Pollitt, A.Y., Veltman, D.M., Morrice, N.A., Machesky, L.M. and Insall, R.H. (2012) Pseudopod growth and evolution during cell movement is controlled through SCAR/WAVE dephosphorylation. Current Biology, 22(7), pp. 553-561. (doi: 10.1016/j.cub.2012.02.020)
Dawson, J.C., Bruche, S., Spence, H.J., Braga, V.M.M. and Machesky, L.M. (2012) Mtss1 promotes cell-cell junction assembly and stability through the small GTPase Rac1. PLoS ONE, 7(3), e31141. (doi: 10.1371/journal.pone.0031141) (PMID:22479308) (PMCID:PMC3313965)
Dawson, J.C., Timpson, P., Kalna, G. and Machesky, L.M. (2012) Mtss1 regulates epidermal growth factor signaling in head and neck squamous carcinoma cells. Oncogene, 31(14), pp. 1781-1793. (doi: 10.1038/onc.2011.376)
Futo, K., Bodis, E., Machesky, L.M. , Nyitrai, M. and Visegrady, B. (2012) Actin controls the I-BAR-membrane interaction. Molecular Biology of the Cell, 23,
García, E., Jones, G.E., Machesky, L.M. and Antón, I.M. (2012) WIP: WASP-interacting proteins at invadopodia and podosomes. European Journal of Cell Biology, 91(11-12), pp. 869-877. (doi: 10.1016/j.ejcb.2012.06.002)
Li, A., Ma, Y., Jin, M., Mason, S., Mort, R.L., Blyth, K. , Larue, L., Sansom, O.J. and Machesky, L.M. (2012) Activated mutant NRasQ61K drives aberrant melanocyte signaling, survival, and invasiveness via a rac1-Dependent mechanism. Journal of Investigative Dermatology, 132(11), pp. 2610-2621. (doi: 10.1038/jid.2012.186)
Li, A. and Machesky, L. M. (2012) Melanoblasts on the move: Rac1 sets the pace. Small GTPases, 3(12), pp. 115-119. (doi: 10.4161/sgtp.19494) (PMID:22790200)
Spence, H.J., Timpson, P., Tang, H.R., Insall, R.H. and Machesky, L.M. (2012) Scar/WAVE3 contributes to motility and plasticity of lamellipodial dynamics but not invasion in three dimensions. Biochemical Journal, 448(1), pp. 35-42. (doi: 10.1042/BJ20112206)
Stevenson, R.P., Veltman, D. and Machesky, L.M. (2012) Actin-bundling proteins in cancer progression at a glance. Journal of Cell Science, 125(5), pp. 1073-1079. (doi: 10.1242/jcs.093799)
Yu, X. and Machesky, L.M. (2012) Cells assemble invadopodia-like structures and invade into matrigel in a matrix metalloprotease dependent manner in the circular invasion assay. PLoS ONE, 7(2), e30605. (doi: 10.1371/journal.pone.0030605) (PMID:22347388) (PMCID:PMC3275555)
Zech, T., Calaminus, S. and Machesky, L. (2012) Actin on trafficking: could actin guide directed receptor transport? Cell Adhesion and Migration, 6(6), pp. 476-481. (doi: 10.4161/cam.21373) (PMID:23076144) (PMCID:PMC3547890)
Li, A. et al. (2011) Rac1 drives melanoblast organization during mouse development by orchestrating pseudopod- driven motility and cell-cycle progression. Developmental Cell, 21(4), pp. 722-734. (doi: 10.1016/j.devcel.2011.07.008)
Thomas, S.G., Calaminus, S.D.J., Machesky, L.M. , Alberts, A.S. and Watson, S.P. (2011) G-protein coupled and ITAM receptor regulation of the formin FHOD1 through Rho Kinase in platelets. Journal of Thrombosis and Haemostasis, 9(8), pp. 1648-1651. (doi: 10.1111/j.1538-7836.2011.04357.x)
Machesky, L. (2011) Going on a (membrane) bender to the F-BAR. Developmental Cell, 20(1), e3. (doi: 10.1016/j.devcel.2010.11.023)
Carnell, M., Zech, T., Calaminus, S.D., Ura, S., Hagedorn, M., Johnston, S.A., May, R.C., Soldati, T., Machesky, L.M. and Insall, R.H. (2011) Actin polymerization driven by WASH causes V-ATPase retrieval and vesicle neutralization before exocytosis. Journal of Cell Biology, 193(5), pp. 831-839. (doi: 10.1083/jcb.201009119)
Lindsay, C.C.R. et al. (2011) P-Rex1 is required for efficient melanoblast migration and melanoma metastasis. Nature Communications, 2, pp. 1-9. (doi: 10.1038/ncomms1560)
Ramsay, A.K. et al. (2011) ERK5 signalling in prostate cancer promotes an invasive phenotype. British Journal of Cancer, 104(4), pp. 664-672. (doi: 10.1038/sj.bjc.6606062)
Vartiainen, M., Heath, R.J.W., Leong, J.M., Visegrády, B., Machesky, L.M. and Xavier, R.J. (2011) Bacterial and host determinants of MAL activation upon EPEC infection: the roles of Tir, ABRA, and FLRT3. PLoS Pathogens, 7(4), e1001332. (doi: 10.1371/journal.ppat.1001332)
Veltman, D.W., Auciello, G., Spence, H.J., Machesky, L.M. , Rappoport, J.Z. and Insall, R.H. (2011) Functional analysis of Dictyostelium IBARa reveals a conserved role of the I-BAR domain in endocytosis. Biochemical Journal, 436(1), pp. 45-52. (doi: 10.1042/BJ20101684)
Zech, T., Calaminus, S.D.J., Caswell, P., Spence, H.J., Carnell, M., Insall, R.H. , Norman, J. and Machesky, L.M. (2011) The Arp2/3 activator WASH regulates 5 1-integrin-mediated invasive migration. Journal of Cell Science, 124(22), pp. 3753-3759. (doi: 10.1242/jcs.080986)
Calaminus, S. D., Inman, G. , Ghaevert, C., Sansom, O. , Watson, S. P., Holyoake, T. L. and Machesky, L. (2010) Alterations In Wnt Signalling In the Megakaryocytic Lineage Leads to Bone Marrow Failure and Myelofibrosis. Blood, 116(21), 628. (doi: 10.1182/blood.V116.21.628.628)
Serrels, B., Sandilands, E., Serrels, A., Baillie, G. , Houslay, M. D., Brunton, V. G., Canel, M., Machesky, L. M. , Anderson, K. I. and Frame, M. C. (2010) A complex between FAK, RACK1, and PDE4D5 controls spreading initiation and cancer cell polarity. Current Biology, 20(12), pp. 1086-1092. (doi: 10.1016/j.cub.2010.04.042)
Li, A., Dawson, J. C., Forero-Vargas, M., Spence, H. J., Yu, X., König, I., Anderson, K. and Machesky, L. M. (2010) The Actin-Bundling Protein Fascin Stabilizes Actin in Invadopodia and Potentiates Protrusive Invasion. Current Biology, 20(4), pp. 339-345. (doi: 10.1016/j.cub.2009.12.035) (PMID:20137952) (PMCID:PMC3163294)
Brooks, S. P., Coccia, M., Tang, H. R., Kanuga, N., Machesky, L. M. , Bailly, M., Cheetham, M. E. and Hardcastle, A. J. (2010) The Nance-Horan syndrome protein encodes a functional WAVE homology domain (WHD) and is important for co-ordinating actin remodelling and maintaining cell morphology. Human Molecular Genetics, 19(12), pp. 2421-2432. (doi: 10.1093/hmg/ddq125)
Machesky, L. M. and Li, A. (2010) Fascin: Invasive filopodia promoting metastasis. Communicative and Integrative Biology, 3(3), pp. 263-270. (doi: 10.4161/cib.3.3.11556) (PMID:20714410) (PMCID:PMC2918773)
Scott, R.W. et al. (2010) LIM kinases are required for invasive path generation by tumor and tumor-associated stromal cells. Journal of Cell Biology, 191(1), pp. 169-185. (doi: 10.1083/jcb.201002041)
Stein, T. et al. (2010) Loss of Reelin Expression in Breast Cancer Is Epigenetically Controlled and Associated with Poor Prognosis. American Journal of Pathology, 177(5), pp. 2323-2333. (doi: 10.2353/ajpath.2010.100209)
Visegrády, B. and Machesky, L.M. (2010) Myopathy-causing actin mutations promote defects in serum-response factor signalling. Biochemical Journal, 427(1), pp. 41-48. (doi: 10.1042/BJ20091641)
Mardakheh, F. K., Yekezare, M., Machesky, L. M. and Heath, J. K. (2009) Spred2 interaction with the late endosomal protein NBR1 down-regulates fibroblast growth factor receptor signaling. Journal of Cell Biology, 187(2), pp. 265-277. (doi: 10.1083/jcb.200905118) (PMID:19822672) (PMCID:PMC2768835)
Vandamme, D., Lambert, E., Waterschoot, D., Tondeleir, D., Vandekerckhove, J., Machesky, L. M. , Constantin, B., Rommelaere, H. and Ampe, C. (2009) Phenotypes induced by NM causing α-skeletal muscle actin mutants in fibroblasts, Sol 8 myoblasts and myotubes. BMC Research Notes, 2, 40. (doi: 10.1186/1756-0500-2-40) (PMID:19284548) (PMCID:PMC2657152)
Insall, R. and Machesky, L.M. (2009) Actin Dynamics at the Leading Edge: From Simple Machinery to Complex Networks. Developmental Cell, 17(3), pp. 310-322. (doi: 10.1016/j.devcel.2009.08.012)
Machesky, L.M. and Tang, H.R. (2009) Actin-Based Protrusions: Promoters or Inhibitors of Cancer Invasion? Cancer Cell, 16(1), pp. 5-7. (doi: 10.1016/j.ccr.2009.06.009)
Mardakheh, F.K., Yekezare, M., Machesky, L.M. and Heath, J.K. (2008) SPRED redirects activated receptors to lysosomes via scaffolding protein NBR1. European Journal of Cancer Supplements, 6(9), pp. 38-39. (doi: 10.1016/S1359-6349(08)71324-7)
Calaminus, S.D.J., Thomas, S., McCarty, O. J. T., Machesky, L.M. and Watson, S. P. (2008) Identification of a novel, actin-rich structure, the actin nodule, in the early stages of platelet spreading. Journal of Thrombosis and Haemostasis, 6(11), pp. 1944-1952. (doi: 10.1111/j.1538-7836.2008.03141.x)
Heung, M., Visegrady, B., Futterer, K. and Machesky, L. (2008) Identification of the insulin-responsive tyrosine phosphorylation sites on IRSp53. European Journal of Cell Biology, 87(8-9), pp. 699-708. (doi: 10.1016/j.ejcb.2008.02.010)
Johnston, S.A., Bramble, J.P., Yeung, C.L., Mendes, P.M. and Machesky, L.M. (2008) Arp2/3 complex activity in filopodia of spreading cells. BMC Cell Biology, 9(1), p. 65. (doi: 10.1186/1471-2121-9-65)
Machesky, L. (2008) Lamellipodia and filopodia in metastasis and invasion. FEBS Letters, 582(14), pp. 2102-2111. (doi: 10.1016/j.febslet.2008.03.039)
Machesky, L. , Jurdic, P. and Hinz, B. (2008) Grab, stick, pull and digest: the functional diversity of actin-associated matrix-adhesion structures. Workshop on Invadopodia, Podosomes and Focal Adhesions in Tissue Invasion. EMBO Reports, 9(2), pp. 139-143. (doi: 10.1038/sj.embor.7401162)
Zech, T. and Machesky, L. (2008) Rab5 and Rac Team Up in Cell Motility. Cell, 134(1), pp. 18-20. (doi: 10.1016/j.cell.2008.06.039)
Thomas, S.G., Calaminus, S.D., Auger, J.M., Watson, S.P. and Machesky, L. M. (2007) Studies on the actin-binding protein HS1 in platelets. BMC Cell Biology, 8, 46. (doi: 10.1186/1471-2121-8-46) (PMID:17996076) (PMCID:PMC2203996)
Calaminus, S. D. J., Auger, J. M., McCarty, O. J. T., Wakelam, M. J. O., Machesky, L. M. and Watson, S. P. (2007) MyosinIIa contractility is required for maintenance of platelet structure during spreading on collagen and contributes to thrombus stability. Journal of Thrombosis and Haemostasis, 5(10), pp. 2136-2145. (doi: 10.1111/j.1538-7836.2007.02696.x) (PMID:17645784)
Bacon, C., Lakics, V., Machesky, L. and Rumsby, M. (2007) N-WASP regulates extension of filopodia and processes by oligodendrocyte progenitors, oligodendrocytes, and Schwann cells—implications for axon ensheathment at myelination. Glia, 55(8), pp. 844-858. (doi: 10.1002/glia.20505)
Fütterer, K. and Machesky, L. M. (2007) “Wunder” F-BAR domains: going from pits to vesicles. Cell, 129(4), pp. 655-657. (doi: 10.1016/j.cell.2007.05.006) (PMID:17512400)
Millard, T. H., Dawson, J. and Machesky, L. M. (2007) Characterisation of IRTKS, a novel IRSp53/MIM family actin regulator with distinct filament bundling properties. Journal of Cell Science, 120(9), pp. 1663-1672. (doi: 10.1242/jcs.001776) (PMID:17430976)
Calaminus, S.D.J., McCarty, O.J.T., Auger, J.M., Pearce, A.C., Insall, R.H., Watson, S.P. and Machesky, L.M. (2007) A major role for Scar/WAVE-1 downstream of GPVI in platelets. Journal of Thrombosis and Haemostasis, 5(3), pp. 535-541. (doi: 10.1111/j.1538-7836.2007.02377.x) (PMID:17319906) (PMCID:PMC1880834)
Legg, J. A., Bompard, G., Dawson, J., Morris, H. L., Andrew, N., Cooper, L., Johnston, S. A., Tramountanis, G. and Machesky, L. (2007) N-WASP involvement in dorsal ruffle formation in mouse embryonic fibroblasts. Molecular Biology of the Cell, 18(2), pp. 678-687. (doi: 10.1091/mbc.e06-06-0569) (PMID:17182853) (PMCID:PMC1783773)
Bathe, F. S., Rommelaere, H. and Machesky, L. M. (2007) Phenotypes of myopathy-related actin mutants in differentiated C2C12 myotubes. BMC Cell Biology, 8, 2. (doi: 10.1186/1471-2121-8-2) (PMID:17227580) (PMCID:PMC1779783)
Uhrig, J. F., Mutondo, M., Zimmermann, L., Deeks, M. J., Machesky, L. M. , Thomas, P., Uhrig, S., Rambke, C., Hussey, P. J. and Hulskamp, M. (2007) The role of Arabidopsis SCAR genes in ARP2-ARP3-dependent cell morphogenesis. Development, 134(5), pp. 967-977. (doi: 10.1242/dev.02792) (PMID:17267444)
Machesky, L. M. and Johnston, S. A. (2007) MIM: a multifunctional scaffold protein. Journal of Molecular Medicine, 85, pp. 569-576. (doi: 10.1007/s00109-007-0207-0) (PMID:17497115)
Baldassarre, M., Ayala, I., Beznoussenko, G., Giacchetti, G., Machesky, L. M. , Luini, A. and Buccione, R. (2006) Actin dynamics at sites of extracellular matrix degradation. European journal of cell biology, 85(12), pp. 1217-1231. (doi: 10.1016/j.ejcb.2006.08.003) (PMID:17010475)
Dawson, J. C., Legg, J. A. and Machesky, L. M. (2006) Bar domain proteins: a role in tubulation, scission and actin assembly in clathrin-mediated endocytosis. Trends in cell biology, 16(10), pp. 493-498. (doi: 10.1016/j.tcb.2006.08.004) (PMID:16949824)
ten Klooster, J. P., Evers, E. E., Janssen, L., Machesky, L. M. , Michiels, F., Hordijk, P. and Collard, J. G. (2006) Interaction between Tiam1 and the Arp2/3 complex links activation of Rac to actin polymerization. Biochemical Journal, 397(1), pp. 39-45. (doi: 10.1042/BJ20051957) (PMID:16599904) (PMCID:PMC1479755)
McCarty, O. J. T., Calaminus, S. D. J., Berndt, M. C., Machesky, L. M. and Watson, S. P. (2006) von Willebrand factor mediates platelet spreading through glycoprotein Ib and αIIbβ3 in the presence of botrocetin and ristocetin, respectively. Journal of Thrombosis and Haemostasis, 4(6), pp. 1367-1378. (doi: 10.1111/j.1538-7836.2006.01966.x) (PMID:16706984)
Ardern, H., Sandilands, E., Machesky, L.M. , Timpson, P., Frame, M.C. and Brunton, V.G. (2006) Src-dependent phosphorylation of Scar1 promotes its association with the Arp2/3 complex. Cell Motility and the Cytoskeleton, 63(1), pp. 6-13. (doi: 10.1002/cm.20101)
Deeks, M. J., Cvrcková, F., Machesky, L. M. , Mikitová, V., Ketelaar, T., Zársky, V., Davies, B. and Hussey, P. J. (2005) Arabidopsis group le formins localize to specific cell membrane domains, interact with actin-binding proteins and cause defects in cell expansion upon aberrant expression. New Phytologist, 168(3), pp. 529-540. (doi: 10.1111/j.1469-8137.2005.01582.x) (PMID:16313636)
Bompard, G., Sharp, S. J., Freiss, G. and Machesky, L. M. (2005) Involvement of Rac in actin cytoskeleton rearrangements induced by MIM-B. Journal of Cell Science, 118(22), pp. 5393-5403. (doi: 10.1242/jcs.02640) (PMID:16280553)
McCarty, O. J. T. et al. (2005) Rac1 is essential for platelet lamellipodia formation and aggregate stability under flow. Journal of Biological Chemistry, 280(47), pp. 39474-39484. (doi: 10.1074/jbc.M504672200) (PMID:16195235) (PMCID:PMC1395485)
Oda, A. et al. (2005) WAVE/Scars in platelets. Blood, 105(8), pp. 3141-3148. (doi: 10.1182/blood-2003-04-1319) (PMID:15280206)
Parsons, M. et al. (2005) Spatially distinct binding of Cdc42 to PAK1 and N-WASP in breast carcinoma cells. Molecular and Cellular Biology, 25(5), pp. 1680-1695. (doi: 10.1128/MCB.25.5.1680-1695.2005) (PMID:15713627) (PMCID:PMC549353)
Millard, T. H., Bompard, G., Heung, M. Y., Dafforn, T. R., Scott, D. J., Machesky, L. M. and Fütterer, K. (2005) Structural basis of filopodia formation induced by the IRSp53/MIM homology domain of human IRSp53. EMBO Journal, 24(2), pp. 240-250. (doi: 10.1038/sj.emboj.7600535) (PMID:15635447) (PMCID:PMC545821)
Stovold, C. F., Millard, T. H. and Machesky, L. M. (2005) Inclusion of Scar/WAVE3 in a similar complex to Scar/WAVE1 and 2. BMC Cell Biology, 6, 11. (doi: 10.1186/1471-2121-6-11) (PMID:15752430) (PMCID:PMC555569)
Legg, J. A. and Machesky, L. M. (2004) MRL proteins: leading Ena/VASP to Ras GTPases. Nature Cell Biology, 6(11), pp. 1015-1017. (doi: 10.1038/ncb1104-1015) (PMID:15516992)
Clarkson, E., Costa, C. F. and Machesky, L. M. (2004) Congenital myopathies: diseases of the actin cytoskeleton. Journal of Pathology, 204(4), pp. 407-417. (doi: 10.1002/path.1648) (PMID:15495263)
Unsworth, K. E., Way, M., McNiven, M., Machesky, L. and Holden, D. W. (2004) Analysis of the mechanisms of Salmonella-induced actin assembly during invasion of host cells and intracellular replication. Cellular microbiology, 6(11), pp. 1041-1055. (doi: 10.1111/j.1462-5822.2004.00417.x) (PMID:15469433)
McCarty, O. J. T., Zhao, Y., Andrew, N., Machesky, L. M. , Staunton, D., Frampton, J. and Watson, S. P. (2004) Evaluation of the role of platelet integrins in fibronectin-dependent spreading and adhesion. Journal of Thrombosis and Haemostasis, 2(10), pp. 1823-1833. (doi: 10.1111/j.1538-7836.2004.00925.x) (PMID:15456495)
Costa, C. F., Rommelaere, H., Waterschoot, D., Sethi, K. K., Nowak, K. J., Laing, N. G., Ampe, C. and Machesky, L. M. (2004) Myopathy mutations in α-skeletal-muscle actin cause a range of molecular defects. Journal of Cell Science, 117(15), pp. 3367-3377. (doi: 10.1242/jcs.01172) (PMID:15226407)
Insall, R. H. and Machesky, L. M. (2004) Regulation of WASP: PIP2 pipped By Toca-1? Cell, 118(2), pp. 140-141. (doi: 10.1016/j.cell.2004.07.005) (PMID:15260983)
Millard, T. H., Sharp, S. J. and Machesky, L. M. (2004) Signalling to actin assembly via the WASP (Wiskott-Aldrich syndrome protein)-family proteins and the Arp2/3 complex. Biochemical Journal, 380(1), pp. 1-17. (doi: 10.1042/bj20040176) (PMID:15040784) (PMCID:PMC1224166)
Vartiainen, M. K. and Machesky, L. M. (2004) The WASP–Arp2/3 pathway: genetic insights. Current Opinion in Cell Biology, 16(2), pp. 174-181. (doi: 10.1016/j.ceb.2004.02.004) (PMID:15196561)
Millard, T.H., Bompard, G., Sharp, S.J. and Machesky, L.M. (2004) Regulation of actin dynamics by the IRSp53 and MIM family of proteins. Molecular Biology of the Cell, 15, 387A-387A.
Singh, S., Powell, D. W., Rane, M. J., Millard, T. H., Trent, J. O., Pierce, W. M., Klein, J. B., Machesky, L. M. and McLeish, K. R. (2003) Identification of the p16-Arc subunit of the Arp 2/3 complex as a substrate of MAPK-activated protein kinase 2 by proteomic analysis. Journal of Biological Chemistry, 278(38), pp. 36410-36417. (doi: 10.1074/jbc.M306428200) (PMID:12829704)
Launay, S., Giannı̀, M., Diomede, L., Machesky, L. M. , Enoug, J. and Papp, B. (2003) Enhancement of ATRA-induced cell differentiation by inhibition of calcium accumulation into the endoplasmic reticulum: cross-talk between RARα and calcium-dependent signaling. Blood, 101(8), pp. 3220-3228. (doi: 10.1182/blood-2002-09-2730) (PMID:12515718)
Launay, S., Brown, G. and Machesky, L. M. (2003) Expression of WASP and Scar1/WAVE1 actin-associated proteins is differentially modulated during differentiation of HL-60 cells. Cell Motility and the Cytoskeleton, 54(4), pp. 274-285. (doi: 10.1002/cm.10101) (PMID:12601690)
Woodings, J. A., Sharp, S. J. and Machesky, L. M. (2003) MIM-B, a putative metastasis suppressor protein, binds to actin and to protein tyrosine phosphatase delta. Biochemical Journal, 371(2), pp. 463-471. (doi: 10.1042/bj20021962) (PMID:12570871) (PMCID:PMC1223315)
Oda, A. et al. (2003) CrkL directs ASAP1 to peripheral focal adhesions. Journal of Biological Chemistry, 278(8), pp. 6456-6460. (doi: 10.1074/jbc.M210817200) (PMID:12522101)
Anderson, S. I., Behrendt, B., Machesky, L. M. , Insall, R. H. and Nash, G. B. (2003) Linked regulation of motility and integrin function in activated migrating neutrophils revealed by interference in remodelling of the cytoskeleton. Cell Motility and the Cytoskeleton, 54(2), pp. 135-146. (doi: 10.1002/cm.10091) (PMID:12529859)
Machesky, L. M. and Bornens, M. (2003) Cell structure and dynamics. Current Opinion in Cell Biology, 15(1), pp. 2-5. (doi: 10.1016/S0955-0674(02)00016-9) (PMID:12517697)
Magdalena, J., Millard, T. H., Etienne-Manneville, S., Launay, S., Warwick, H. K. and Machesky, L. M. (2003) Involvement of the Arp2/3 complex and Scar2 in Golgi polarity in scratch wound models. Molecular Biology of the Cell, 14(2), pp. 670-684. (doi: 10.1091/mbc.e02-06-0345) (PMID:12589062) (PMCID:PMC150000)
Magdalena, J., Millard, T. H. and Machesky, L. M. (2003) Microtubule involvement in NIH 3T3 Golgi and MTOC polarity establishment. Journal of Cell Science, 116(4), pp. 743-756. (doi: 10.1242/jcs.00288) (PMID:12538774)
Millard, T. H., Behrendt, B., Launay, S., Fütterer, K. and Machesky, L. M. (2003) Identification and characterisation of a novel human isoform of Arp2/3 complex subunit p16-ARC/ARPC5. Cell Motility and the Cytoskeleton, 54(1), pp. 81-90. (doi: 10.1002/cm.10087) (PMID:12451597)
Rombouts, K., Knittel, T., Machesky, L. , Braet, F., Wielant, A., Hellemans, K., De Bleser, P., Gelman, I., Ramadori, G. and Geerts, A. (2002) Actin filament formation, reorganization and migration are impaired in hepatic stellate cells under influence of trichostatin A, a histone deacetylase inhibitor. Journal of Hepatology, 37(6), pp. 788-796. (doi: 10.1016/S0168-8278(02)00275-1) (PMID:12445420)
Olazabal, I. M., Caron, E., May, R. C., Schilling, K., Knecht, D. A. and Machesky, L. M. (2002) Rho-kinase and myosin-II control phagocytic cup formation during CR, but not FcγR, phagocytosis. Current Biology, 12(16), pp. 1413-1418. (doi: 10.1016/S0960-9822(02)01069-2) (PMID:12194823)
Machesky, L. M. (2002) Sharks' teeth and dunes. Nature, 417, pp. 495-497. 6888. (doi: 10.1038/417494a) (PMID:12037547)
Machesky, L. and Burd, G. (2002) Cytoskeleton signalling: 7 November 2001. Biochemist, 24(1), p. 32. (doi: 10.1042/BIO02401032)
Insall, R. , Müller-Taubenberger, A., Machesky, L. , Köhler, J., Simmeth, E., Atkinson, S. J., Weber, I. and Gerisch, G. (2001) Dynamics of the Dictyostelium Arp2/3 complex in endocytosis, cytokinesis, and chemotaxis. Cell Motility and the Cytoskeleton, 50(3), pp. 115-128. (doi: 10.1002/cm.10005) (PMID:11807934)
Elliott, D. A., Coleman, D. J., Lane, M. A., May, R. C., Machesky, L. M. and Clark, D. P. (2001) Cryptosporidium parvum infection requires host cell actin polymerization. Infection and Immunity, 69(9), pp. 5940-5942. (doi: 10.1128/iai.69.9.5940-5942.2001) (PMID:11500478) (PMCID:PMC98718)
May, R. C. and Machesky, L. M. (2001) Plagiarism and pathogenesis: common themes in actin remodeling. Developmental Cell, 1(3), pp. 317-318. (doi: 10.1016/S1534-5807(01)00051-X) (PMID:11702942)
Olazabal, I. M. and Machesky, L. M. (2001) Abp1p and cortactin, new “hand-holds” for actin. Journal of Cell Biology, 154(4), pp. 679-682. (doi: 10.1083/jcb.200105061) (PMID:11514584) (PMCID:PMC2196464)
Burns, S., Thrasher, A. J., Blundell, M. P., Machesky, L. and Jones, G. E. (2001) Configuration of human dendritic cell cytoskeleton by Rho GTPases, the WAS protein, and differentiation. Blood, 98(4), pp. 1142-1149. (doi: 10.1182/blood.v98.4.1142) (PMID:11493463)
Machesky, L. M. , Insall, R. H. and Volkman, L. E. (2001) WASP homology sequences in baculoviruses. Trends in Cell Biology, 11(7), pp. 286-287. (doi: 10.1016/S0962-8924(01)02009-8) (PMID:11434350)
Bailly, M., Ichetovkin, I., Grant, W., Zebda, N., Machesky, L. M. , Segall, J. E. and Condeelis, J. (2001) The F-actin side binding activity of the Arp2/3 complex is essential for actin nucleation and lamellipod extension. Current Biology, 11(8), pp. 620-625. (doi: 10.1016/S0960-9822(01)00152-X) (PMID:11369208)
Millard, T. H. and Machesky, L. M. (2001) The Wiskott-Aldrich syndrome protein (WASP) family. Trends in Biochemical Sciences, 26(3), pp. 189-199. (doi: 10.1016/S0968-0004(01)01788-1) (PMID:11246027)
May, R.C. and Machesky, L.M. (2001) Phagocytosis and the actin cytoskeleton. Journal of Cell Science, 114(6), pp. 1061-1077. (PMID:11228151)
Stinchcombe, J. C., Barral, D. C., Mules, E. H., Booth, S., Hume, A. N., Machesky, L. M. , Seabra, M. C. and Griffiths, G. M. (2001) Rab27a is required for regulated secretion in cytotoxic T lymphocytes. Journal of Cell Biology, 152(4), pp. 825-834. (doi: 10.1083/jcb.152.4.825) (PMID:11266472) (PMCID:PMC2195783)
Timpson, P., Carragher, N. O., Machesky, L. and Frame, M. C. (2001) Association of scar with the SRC tyrosine kinase: consequences for scar regulation. British Journal of Cancer, 85, p. 22. (doi: 10.1054/bjoc.2001.1917) (PMCID:PMC2557556)
Insall, R. and Machesky, L. (2001) Cytoskeleton. Encyclopedia of Life Sciences, (doi: 10.1038/npg.els.0001252)
Rombouts, K., Knittel, T., Wielant, A., Piscaglia, F., Machesky, L. , Hellemans, K., Gelman, I., Ramadori, G. and Feerts, A. (2001) Effect of trichostatin A, a histone deacetylase inhibitor, on proteins of the actin cytoskeleton. Cells of the Hepatic Sinusoid, 8, pp. 271-273.
Pistor, S., Grobe, L., Sechi, A.S., Domann, E., Gerstel, B., Machesky, L.M. , Chakraborty, T. and Wehland, J. (2000) Mutations of arginine residues within the 146-KKRRK-150 motif of the ActA protein of Listeria monocytogenes abolish intracellular motility by interfering with the recruitment of the Arp2/3 complex. Journal of Cell Science, 113(18), pp. 3277-3287. (PMID:10954425)
Machesky, L. M. (2000) Putting on the brakes: a negative regulatory function for Ena/VASP proteins in cell migration. Cell, 101(7), pp. 685-688. (doi: 10.1016/S0092-8674(00)80879-X) (PMID:10892738)
May, R. C., Caron, E., Hall, A. and Machesky, L. M. (2000) Involvement of the Arp2/3 complex in phagocytosis mediated by FcγR or CR3. Nature Cell Biology, 2(4), pp. 246-248. (doi: 10.1038/35008673) (PMID:10783245)
Rozelle, A.L., Machesky, L.M. , Yamamoto, M., Driessens, M.H.E., Insall, R.H. , Roth, M.G., Luby-Phelps, K., Marriott, G., Hall, A. and Yin, H.L. (2000) Phosphatidylinositol 4,5-bisphosphate induces actin-based movement of raft-enriched vesicles through WASP-Arp2/3. Current Biology, 10(6), pp. 311-320. (doi: 10.1016/S0960-9822(00)00384-5) (PMID:10744973)
Machesky, L. M. (2000) The tails of two myosins. Journal of Cell Biology, 148(2), pp. 219-221. (doi: 10.1083/jcb.148.2.219) (PMID:10648552) (PMCID:PMC2174283)
Machesky, L. M. and Schliwa, M. (2000) Cell dynamics: a new look at the cytoskeleton. Nature Cell Biology, 2(1), E17-E18. (doi: 10.1038/71403) (PMID:10620818)
Machesky, L. M. , May, R., Olarreaga, I. M. O., Launay, S., Hall, M. E. and Behrendt, B. (2000) Signalling to the actin cytoskeleton via the WASP-family proteins and the Arp2/3 complex. Molecular Biology of the Cell, 11, 3A.
Mullins, R. D. and Machesky, L. M. (2000) Actin assembly mediated by Arp2/3 complex and WASP family proteins. Methods in Enzymology, 325, pp. 214-237. (doi: 10.1016/s0076-6879(00)25445-1) (PMID:11036606)
Machesky, L. M. and Cooper, J. A. (1999) Cell motility: Bare bones of the cytoskeleton. Nature, 401(6753), pp. 542-543. (doi: 10.1038/44044) (PMID:10524617)
Machesky, L. (1999) Short and not so sweet. Current Biology, 9(17), R623. (doi: 10.1016/s0960-9822(99)80403-5) (PMID:10508596)
Machesky, L. M. and Insall, R. H. (1999) Signaling to actin dynamics. Journal of Cell Biology, 146(2), pp. 267-272. (doi: 10.1083/jcb.146.2.267) (PMID:10427083) (PMCID:PMC2156183)
May, R. C., Hall, M. E., Higgs, H. N., Pollard, T. D., Chakraborty, T., Wehland, J., Machesky, L. M. and Sechi, A. S. (1999) The Arp2/3 complex is essential for the actin-based motility of Listeria monocytogenes. Current Biology, 9(14), pp. 759-762. (doi: 10.1016/S0960-9822(99)80337-6) (PMID:10421578)
Insall, R. and Machesky, L. (1999) PH domains in WASP – a bug in the system? Trends in Cell Biology, 9(6), p. 211. (doi: 10.1016/S0962-8924(99)01569-X) (PMID:10354565)
Machesky, L. M. (1999) Rocket-based motility: a universal mechanism? Nature Cell Biology, 1(2), E29-E31. (doi: 10.1038/10020) (PMID:10559888)
Castellano, F., Montcourrier, P., Guillemot, J.-C., Gouin, E., Machesky, L. , Cossart, P. and Chavrier, P. (1999) Inducible recruitment of Cdc42 or WASP to a cell-surface receptor triggers actin polymerization and filopodium formation. Current Biology, 9(7), pp. 351-361. (doi: 10.1016/S0960-9822(99)80161-4) (PMID:10209117)
Tsujioka, M., Machesky, L.M. , Cole, S.L., Yahata, K. and Inouye, K. (1999) A unique talin homologue with a villin headpiece-like domain is required for multicellular morphogenesis in Dictyostelium. Current Biology, 9(7), pp. 389-394. (doi: 10.1016/S0960-9822(99)80169-9) (PMID:10209124)
Machesky, L. M. , Mullins, R. D., Higgs, H. N., Kaiser, D. A., Blanchoin, L., May, R. C., Hall, M. E. and Pollard, T. D. (1999) Scar, a WASp-related protein, activates nucleation of actin filaments by the Arp2/3 complex. Proceedings of the National Academy of Sciences of the United States of America, 96(7), pp. 3739-3744. (doi: 10.1073/pnas.96.7.3739) (PMID:10097107) (PMCID:PMC22364)
Machesky, L. M. and Gould, K. L. (1999) The Arp2/3 complex: a multifunctional actin organizer. Current Opinion in Cell Biology, 11(1), pp. 117-121. (doi: 10.1016/S0955-0674(99)80014-3) (PMID:10047519)
Braga, V. M.M., Del Maschio, A., Machesky, L. and Dejana, E. (1999) Regulation of cadherin function by Rho and Rac: modulation by junction maturation and cellular context. Molecular Biology of the Cell, 10(1), pp. 9-22. (doi: 10.1091/mbc.10.1.9) (PMID:9880323) (PMCID:PMC25150)
Rombouts, K., Knittel, T., Wielant, A., Hellemans, K., Gelman, I., Machesky, L. , Ramadori, G. and Gerts, A. (1999) Trichostatin A suppresses transdifferentiation of rat stellate cells by its effect on the assembly pathway of the actin cytoskeleton. Hepatology, 30(4), 492A.
Machesky, L. M. and Insall, R. H. (1998) Scar1 and the related Wiskott–Aldrich syndrome protein, WASP, regulate the actin cytoskeleton through the Arp2/3 complex. Current Biology, 8(25), pp. 1347-1356. (doi: 10.1016/S0960-9822(98)00015-3) (PMID:9889097)
Schafer, D. A., Welch, M. D., Machesky, L. M. , Bridgman, P. C., Meyer, S. M. and Cooper, J. A. (1998) Visualization and molecular analysis of actin assembly in living cells. Journal of Cell Biology, 143(7), pp. 1919-1930. (doi: 10.1083/jcb.143.7.1919) (PMID:9864364) (PMCID:PMC2175235)
Machesky, L. M. and Way, M. (1998) Actin branches out. Nature, 394(6689), pp. 125-126. (doi: 10.1038/28039) (PMID:9671292)
Machesky, L. M. , Insall, R. H. and Kay, R. R. (1998) The helC gene encodes a putative DEAD-box RNA helicase required for development in Dictyostelium discoideum. Current Biology, 8(10), 607-S4. (doi: 10.1016/S0960-9822(98)70233-7) (PMID:9601648)
Machesky, L. M. (1998) Cytokinesis: IQGAPs find a function. Current Biology, 8(6), R202-R205. (doi: 10.1016/s0960-9822(98)70125-3) (PMID:9512410)
Machesky, L. M. , Reeves, E., Wietnjes, F., Mattheyse, F. J., Grogan, A., Totty, N. F., Burlingame, A. L., Hsuan, J. J. and Segal, A. W. (1997) Mammalian actin-related protein 2/3 complex localizes to regions of lamellipodial protrusion and is composed of evolutionarily conserved proteins. Biochemical Journal, 328(1), pp. 105-112. (doi: 10.1042/bj3280105) (PMID:9359840) (PMCID:PMC1218893)
Machesky, L. M. and Hall, A. (1997) Role of actin polymerization and adhesion to extracellular matrix in Rac-and Rho-induced cytoskeletal reorganization. Journal of Cell Biology, 138(4), pp. 913-926. (doi: 10.1083/jcb.138.4.913) (PMID:9265656) (PMCID:PMC2138040)
Tuxworth, R. I., Cheetham, J. L., Machesky, L. M. , Spiegelmann, G. B., Weeks, G. and Insall, R. H. (1997) Dictyostelium RasG is required for normal motility and cytokinesis, but not growth. Journal of Cell Biology, 138(3), pp. 605-614. (doi: 10.1083/jcb.138.3.605) (PMID:9245789) (PMCID:PMC2141629)
Braga, V. M.M., Machesky, L. M. , Hall, A. and Hotchin, N. A. (1997) The small GTPases Rho and Rac are required for the establishment of cadherin-dependent cell-cell contacts. Journal of Cell Biology, 137(6), pp. 1421-1431. (doi: 10.1083/jcb.137.6.1421) (PMID:9182672) (PMCID:PMC2132529)
Machesky, L. M. (1997) Cell motility: complex dynamics at the leading edge. Current Biology, 7(3), R164-R167. (doi: 10.1016/S0960-9822(97)70079-4) (PMID:9162480)
Machesky, L. M. and Hall, A. (1996) Rho: a connection between membrane receptor signalling and the cytoskeleton. Trends in Cell Biology, 6(8), pp. 304-310. (doi: 10.1016/0962-8924(96)10026-X) (PMID:15157438)
Petrella, E. C., Machesky, L. M. , Kaiser, D. A. and Pollard, T. D. (1996) Structural requirements and thermodynamics of the interaction of proline peptides with profilin. Biochemistry, 35(51), pp. 16535-16543. (doi: 10.1021/bi961498d) (PMID:8987987)
Doberstein, S.K., Wiegand, G., Machesky, L.M. and Pollard, T.D. (1995) Fluorescent erythrocyte ghosts as standards for quantitative flow cytometry. Cytometry, 20(1), pp. 14-18. (doi: 10.1002/cyto.990200104) (PMID:7600895)
Machesky, L.M. , Atkinson, S.J., Ampe, C., Vandekerckhove, J. and Pollard, T.D. (1994) Purification of a cortical complex containing two unconventional actins from Acanthamoeba by affinity chromatography on profilin-agarose. Journal of Cell Biology, 127(1), pp. 107-115. (doi: 10.1083/jcb.127.1.107) (PMID:7929556) (PMCID:PMC2120189)
Machesky, L. M. , Cole, N. B., Moss, B. and Pollard, T. D. (1994) Vaccinia virus expresses a novel profilin with a higher affinity for polyphosphoinositides than actin. Biochemistry, 33(35), pp. 10815-10824. (doi: 10.1021/bi00201a032) (PMID:8075084)
Machesky, L. M. and Pollard, T. D. (1993) Profilin as a potential mediator of membrane-cytoskeleton communication. Trends in Cell Biology, 3(11), pp. 381-385. (doi: 10.1016/0962-8924(93)90087-h) (PMID:14731655)
Machesky, L.M. (1992) Phosphorylation of phospholipase-γ l, profilin and tyrosine. Biomedicine and Pharmacotherapy, 46(2-3), pp. 128-129. (doi: 10.1016/0753-3322(92)90287-H)
Goldschmidt-Clermont, P.J., Machesky, L.M. , Doberstein, S.K. and Pollard, T.D. (1991) Mechanism of the interaction of human platelet profilin with actin. Journal of Cell Biology, 113(5), pp. 1081-1089. (doi: 10.1083/jcb.113.5.1081) (PMID:1645736) (PMCID:PMC2289016)
Goldschmidt-Clermont, P.J., Kim, J.W., Machesky, L.M. , Rhee, S.G. and Pollard, T.D. (1991) Regulation of phospholipase C-gamma 1 by profilin and tyrosine phosphorylation. Science, 251(4998), pp. 1231-1233. (doi: 10.1126/science.1848725) (PMID:1848725)
Machesky, L.M. , Goldschmidt-Clermont, P.J. and Pollard, T.D. (1990) The affinities of human platelet and Acanthamoeba profilin isoforms for polyphosphoinositides account for their relative abilities to inhibit phospholipase C. Cell Regulation, 1(12), pp. 937-950. (doi: 10.1091/mbc.1.12.937) (PMID:1966040) (PMCID:PMC362863)
Goldschmidt-Clermont, P.J., Machesky, L.M. , Baldassare, J.J. and Pollard, T.D. (1990) The actin-binding protein profilin binds to PIP2 and inhibits its hydrolysis by phospholipase C. Science, 247(4950), pp. 1575-1578. (doi: 10.1126/science.2157283) (PMID:2157283)
Kang, Z.C., Machesky, L. , Eick, H.A. and Eyring, L. (1988) The solvolytic disproportionation of mixed-valence compounds: III. Pb3O4. Journal of Solid State Chemistry, 75(1), pp. 73-89. (doi: 10.1016/0022-4596(88)90304-0)
Book Sections
Siebert, S. , Insall, R. and Machesky, L. M. (2019) More means less: managing overflow in science publishing. In: Czarniawska, Barbara and Löfgren, Orvar (eds.) Overwhelmed by Overflows? How People and Organizations Create and Manage Excess. Lund University Press: Lund, pp. 130-150. ISBN 9789198469813 (doi: 10.7765/9789198469813.00013)
Stovold, C. F., Sharp, S. J. and Machesky, L. (2005) Actin filament assembly: the search for a barbed end. In: Ridley, Anne, Peckham, Michelle and Clark, Peter (eds.) Cell Motility: From Molecules to Organisms. Wiley, pp. 135-147. ISBN 9780470094099
Machesky, L. M. and May, R. C. (2001) Arps: actin-related proteins. In: dos Remedios, G. and Thomas, D.D. (eds.) Molecular Interactions of Actin. Springer. ISBN 9783540671107 (doi: 10.1007/978-3-540-46560-7_15)
Pollard, T. D., Machesky, L. and Goldschmidt-Clermont, P. (1991) Interaction of profilins with membrane lipids. In: Mooseker, Mark S. and Morrow, Jon S. (eds.) Ordering the Membrane-Cytoskeleton Trilayer. Series: Current Topics in Membranes (38). Academic Press: San Diego, California ; London, pp. 217-225. ISBN 9780121533380 (doi: 10.1016/S0070-2161(08)60790-X)
Pollard, T. D., Magnus, K. A., Doberstein, S., Goldschmidt-Clermont, P., Kaiser, D. A., Machesky, L. , Maciver, S., Rimm, D. L. and Wachsstock, D. (1989) Structure-function studies of the actin filament system of Acanthamoeba. In: Aebi, Ueli and Engel, Jürgen (eds.) Cytoskeletal and Extracellular Proteins: Structure, Interactions and Assembly The 2nd International EBSA Symposium. Series: Springer series in biophysics (3). Springer: Berlin, Heidelberg, pp. 271-279. ISBN 9783642739279 (doi: 10.1007/978-3-642-73925-5_50)
Conference or Workshop Item
Davidson, A.J., Veltman, D.M., Machesky, L.M. and Insall, R.H. (2012) The Interplay Between SCAR (WAVE) and WASP in Actin-Based Motility. 2012 American Society for Cell Biology Annual Meeting, San Francisco, CA, USA, 15-19 Dec 2012. p. 1823. (doi: 10.1091/mbc.e12-10-0757)
Monteiro, P., Hertzog, M., Rossé, C., Desnos, C., Zech, T., Formstecher, E., Darchen, F., Machesky, L. , Gautreau, A. and Chavrier, P. (2012) Co-ordinated Functions of WASH and Exocyst Complex Underlie the Biogenesis of Invadopodia in Metastatic Breast Tumor Cells. 2012 American Society for Cell Biology Annual Meeting, San Francisco, CA, USA, 15-19 Dec 2012. p. 2077. (doi: 10.1091/mbc.e12-10-0757)
Zech, T. et al. (2012) N-WASP Coordinates the Delivery and F-actin Mediated Reversible Capture of MT1-MMP at Invasive Pseudopods to Drive Breast Cancer Cell Invasion. 2012 American Society for Cell Biology Annual Meeting, San Francisco, CA, USA, 15-19 Dec 2012. p. 2076. (doi: 10.1091/mbc.e12-10-0757)
Doyle, B., Tan, E.H., Timpson, P., Machesky, L.M. , Ridgway, R.R., Jeffery, R.R., Poulsom, R., Morton, J.P. and Sansom, O.J. (2012) The Tumor Suppressor ARF Can Promote Invasion in the Absence of p53 Activity. United States and Canadian Academy of Pathology 101st Annual Meeting, Vancouver, BC, Canada, 17-23 Mar 2012. 461A. (doi: 10.1038/modpathol.2012.27)
Futó, K., Machesky, L. M. and Visegrády, B. (2011) The Role of the I-BAR Domain in the Filopodia Formation. 8th EBSA European Biophysics Congress, Budapest, Hungary, 23-27 Aug 2011. S197. (doi: 10.1007/s00249-011-0734-z)
Machesky, L. M. , Legg, J. A., McCarty, O. J., Calaminus, S. D.J. and Watson, S. P. (2006) Scar/WAVE Proteins in Cell Spreading and Membrane Dynamics. 67th Annual Meeting of the Society of General Physiologists, Woods Hole, MA, USA, 6-10 September 2006. (doi: 10.1085/jgp.128.1.1a)
Deeks, M.J., Kaloriti, D., Machesky, L.M. , Malho, R., Davies, B. and Hussey, P.J. (2005) Actin Nucleators in Plants. Society for Experimental Biology Annual Main Meeting, Barcelona, Spain, 11-15 July 2005. (doi: 10.1016/j.cbpb.2005.05.026)
Machesky, L.M. , Millard, T.H., Bompard, G., Sharp, S.J., Woodings, J.A. and Futterer, K. (2005) Regulation of the Actin Cytoskeleton by IRSp53 and MIM Proteins. IUBMB 50th Anniversary Symposium: Protein Structureand Function, Budapest, Hungary, 2–7 July 2005.
Owen, J. et al. (2005) Rac1 is Essential for Platelet Lamellipodia and Aggregate Stability Under Flow. BSCR Spring Meeting 2005
Grants
Grants and Awards listed are those received whilst working with the University of Glasgow.
- Harnessing the physics of light to reveal mechanisms of multicellular shape and movement
Engineering and Physical Sciences Research Council
2019 - 2022
- Metabolic drivers of pancreatic cancer cell migration and metastasis
Medical Research Council
2018 - 2020
- Investigating the role of N-WASP in the development and metastasis of colorectal cancer (SCP3 Fellowship 2012)
Medical Research Council
2012 - 2017
Research datasets
2022
Nikolaou, S., Juin, A., Whitelaw, J., Paul, N., Fort, L., Nixon, C., Spence, H., Bryson, S. and Machesky, L. (2022) CYRI-B mediated macropinocytosis drives metastasis via lysophosphatidic acid receptor uptake. [Data Collection]
2020
Zickus, V., Wu, M.-L., Morimoto, K., Kapitany, V., Fatima, A., Turpin, A. , Insall, R. , Whitelaw, J., Machesky, L. , Bruschini, C., Faccio, D. and Charbon, E. (2020) Fluorescence lifetime imaging with a megapixel SPAD camera and neural network lifetime estimation. [Data Collection]
Tweedy, L., Thomason, P., Paschke, P., Martin, K., Machesky, L. , Zagnoni, M. and Insall, R. (2020) Seeing around corners: cells solve mazes and respond at a distance using attractant breakdown. [Data Collection]