Cell-culture microscopic stage temperature controller
The combination of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) with the optical platform CellOPTIQ (Clyde Biosciences) has proved to be a useful tool for the early stages of drug-screening and basic electrophysiological research. One inconvenient aspect of using hiPSC-CMs is their spontaneous activity which can vary during assay and affect the underlying action potential duration. The alternative is to correct the action potential for the known effects of rate on action potential characteristics. To address this issue, The Cell-culture microscopic stage temperature controller device will allow culture clusters of hiPSC-CMs on petri dishes and placed in a thermostated atmosphere and stimulate them at difference frequency with graphite electrodes. The system stage has been configured to be fixed on the stage of an inverted microscope and using these approach hiPSC-CMs can be stimulated and membrane potential recorded.
One of the key advantages of the system is the ability of controlling temperature in order to change the final temperature on the cellular media. The temperature settings available range from 19°C to 37°C. It allows performing hypothermia-rewarming studies on the adult cells while they have been stimulated at a fixed frequency. Accurate knowledge of this effect will allow a more accurate interpretation of the temperature effect in the heart and the side effect to expose the heart at cold environment and rewarm up to physiological temperature.
For more detail contact the Biolectronics Unit - Mr Nosrat Mirzai