The course aims to provide students with a critical understanding of the various ways in which large scale protein expression can be carried out in recombinant systems for Biotechnological applications.

Lectures, tutorials and a lab class.

None

None

Lab Assessment (75%)

Group Presentation (25%) - video presentation

Reassessments are normally available for all courses, except those which contribute to the Honours classification. For non-Honours courses, students are offered reassessment in all or any of the components of assessment if the satisfactory (threshold) grade for the overall course is not achieved at the first attempt. This is normally grade D3 for undergraduate students and grade C3 for postgraduate students. Exceptionally it may not be possible to offer reassessment of some coursework items, in which case the mark achieved at the first attempt will be counted towards the final course grade. Any such exceptions for this course are described below. 

Group presentation cannot be reassessed.

The course aims to provide students with a critical understanding of the various ways in which large scale protein expression can be carried out for Biotechnological applications. The course will explore various recombinant systems, the use of coupled proteins and tags, expression of difficult proteins and different methods of affinity purification.

By the end of this course students will be able to:

■ Critically analyse the current methodologies used to express and purify proteins of biotechnological significance in recombinant systems.

■ Critically evaluate the use of different recombinant systems and purification mechanisms.

■ Describe, critique and perform techniques that are commonly used to express proteins in E.coli.

■ Critically analyse results from experimental data and write an accurate laboratory report using these results.

Students must submit at least 75% by weight of the components (including examinations) of the course's summative assessment.