Multiscale Imaging of Host-Pathogen Interactions to Characterise the Effects of Novel Anti-Infective Therapies

Supervisors: 

Gail McConnell, University of Strathclyde 

Andrew Roe, School of Infection and Immunity, University of Glasgow

Rebecca McHugh, School of Infection and Immunity, University of Glasgow

 

Summary: 

This project focuses on developing and applying advanced imaging and data processing techniques to better understand intracellular infections and the impact of innovative treatments.

We will accurately quantify the burden of intracellular pathogens, specifically Salmonella Typhimurium, using novel imaging methods in conjunction with data processing techniques. This will enable researchers to assess the effectiveness of anti-virulence therapies in targeting these pathogens.

We will also use "organ on a chip" technology to study the effects of Shiga Toxin, which is known for causing severe kidney damage. This aim will help to characterize both the macro and microstructural changes in renal tissues when exposed to the toxin, providing insights into its pathological impact on kidney health.

Finally, we will optimize tissue processing and optical clearing techniques for organs harvested from animal models of enteric infections. By improving these protocols, we aim to enhance the clarity and detail of imaging, which will facilitate the study of tissue-level changes during infection.

Training outcomes include mastering cutting-edge imaging technologies, data processing methods, and tissue processing techniques, equipping researchers with the skills to investigate pathogenic processes and evaluate therapeutic interventions.