Developing a Lateral Flow Device (LFD) for Enhanced Detection and Control of Johne’s Disease

Supervisors: 

Stephanie Menzies, Biomedical and Life Sciences, Lancaster University

Irene R Grant, School of Biological Sciences, Queen’s University Belfast

 

Summary: 

This PhD project offers a unique opportunity to develop a rapid diagnostic test for the causative agent of Johne's disease (Mycobacterium avium subspecies paratuberculosis [MAP]), a major concern in the livestock industry for which current detection methods are sub-optimal. The successful candidate will gain hands-on experience in cutting-edge techniques, equipping them with a diverse skill set that is well-suited for careers in translational research, both academia and the biotechnology industry.

The student will gain skills in molecular biology, protein production (of MAP antigens and anti-MAP antibodies), in vitro antibody discovery using yeast-display libraries, antibody characterization and evaluation, and finally lateral flow assay development. By the end of the project, the student will have developed a prototype lateral flow device for Johne's disease, and evaluated the device for sensitivity and specificity to detect MAP in the lab and on field samples. This project provides an exciting opportunity to contribute to the development of a tool with real-world applications to improve animal health, supported by expert supervision and state-of-the-art facilities.